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The map label for this gene is echA8 [H]

Identifier: 154248261

GI number: 154248261

Start: 4813361

End: 4814191

Strand: Reverse

Name: echA8 [H]

Synonym: Xaut_4341

Alternate gene names: 154248261

Gene position: 4814191-4813361 (Counterclockwise)

Preceding gene: 154248266

Following gene: 154248260

Centisome position: 90.68

GC content: 71.72

Gene sequence:

>831_bases
ATGTCGGCTGAGCCATCGGCCCACCCCGTCGCCGGCGCGGCGGCCCCCGTCGTGACGACCAGCCGCGACGGTGGGGTCCT
GACCCTCACCCTGGCGCGGCCGGAAGCGCGCAACAGCCTCTCCGAGGCGATGATGGCGGCGCTCACCTCCGCCCTCGCGG
CCATCGCGGCGGACACCGCCGTGCGCGCGGTGGTGCTGGCGGCGGAAGGCCCGGTCTATTCCGCCGGCCACGACCTCAAG
GAGATCCAGGCCCACCGCGGCGATGCCGACCGGGGCCGGGCCTATTTCGAGGATGTGCTGAAGCGCTGCGCCACGCTGAT
GACCGCCATCGTCCGCCTGCCCCAGCCGGTGATCGCCGCCGTGGAAGGCATGGCCACCGCCGCCGGCTGCCAGCTGGTGG
CGAGCTGCGACCTCGCGGTGGCGGGCGAGAAGGCGCGCTTCTGCACGCCGGGCGTAAACATCGGCCTGTTCTGCCACACC
CCCATGGTGGCGCTCTCGCGCAACCTGGCGCGCAAGCACGCCATGGAGATGCTGCTTTTGGGCGAATGGGTGGAGGCGGA
GGAGGCCCGCCGCATGGGCCTCGTCAACCGCGTGGTGCCGGCCGGCGACGCCCTGTCCACTGCGCAGGCCCTCGCCCGCC
GCATCGCCGAGAAGTCCCAGGTGCCGGTGAAGCTCGGCAAGGCCGCCTTCTACACCCAGATGGAGATGGGCCTCGACGAC
GCCTATGCCTATGCCTCCCGCGTCATGGCCGAGAACATGCTGGCCCGCGATGCCGACGAAGGCATCTCCGCAGTACTCGC
CAAGCGTGCACCCAGCTGGGAGGACCGGTGA

Upstream 100 bases:

>100_bases
CCATCTCCTGCCCCTGACTGCGCTTTGGCCCGGCTCGCACCGGTCGCGTCTTATGCATAGGATAGTACCCTGAAGGTTAA
ACAGAAGAGGGCGCAAACCC

Downstream 100 bases:

>100_bases
CGGGCCTGCCGCGCGAACGGCTCTGACAGCCTTGTTTACCAACCATCAACCATAATCCGCTCAACTCGATGCAGGCTGGT
GCGACTCGCGCCGCCAGCGT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSAEPSAHPVAGAAAPVVTTSRDGGVLTLTLARPEARNSLSEAMMAALTSALAAIAADTAVRAVVLAAEGPVYSAGHDLK
EIQAHRGDADRGRAYFEDVLKRCATLMTAIVRLPQPVIAAVEGMATAAGCQLVASCDLAVAGEKARFCTPGVNIGLFCHT
PMVALSRNLARKHAMEMLLLGEWVEAEEARRMGLVNRVVPAGDALSTAQALARRIAEKSQVPVKLGKAAFYTQMEMGLDD
AYAYASRVMAENMLARDADEGISAVLAKRAPSWEDR

Sequences:

>Translated_276_residues
MSAEPSAHPVAGAAAPVVTTSRDGGVLTLTLARPEARNSLSEAMMAALTSALAAIAADTAVRAVVLAAEGPVYSAGHDLK
EIQAHRGDADRGRAYFEDVLKRCATLMTAIVRLPQPVIAAVEGMATAAGCQLVASCDLAVAGEKARFCTPGVNIGLFCHT
PMVALSRNLARKHAMEMLLLGEWVEAEEARRMGLVNRVVPAGDALSTAQALARRIAEKSQVPVKLGKAAFYTQMEMGLDD
AYAYASRVMAENMLARDADEGISAVLAKRAPSWEDR
>Mature_275_residues
SAEPSAHPVAGAAAPVVTTSRDGGVLTLTLARPEARNSLSEAMMAALTSALAAIAADTAVRAVVLAAEGPVYSAGHDLKE
IQAHRGDADRGRAYFEDVLKRCATLMTAIVRLPQPVIAAVEGMATAAGCQLVASCDLAVAGEKARFCTPGVNIGLFCHTP
MVALSRNLARKHAMEMLLLGEWVEAEEARRMGLVNRVVPAGDALSTAQALARRIAEKSQVPVKLGKAAFYTQMEMGLDDA
YAYASRVMAENMLARDADEGISAVLAKRAPSWEDR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=259, Percent_Identity=46.3320463320463, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI194097323, Length=277, Percent_Identity=30.6859205776173, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI70995211, Length=261, Percent_Identity=29.8850574712644, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=27.34375, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4757968, Length=230, Percent_Identity=24.7826086956522, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI49355787, Length=230, Percent_Identity=24.7826086956522, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=230, Percent_Identity=24.3478260869565, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538581, Length=230, Percent_Identity=24.3478260869565, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=230, Percent_Identity=24.3478260869565, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI51538573, Length=230, Percent_Identity=24.3478260869565, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI45643119, Length=201, Percent_Identity=27.8606965174129, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI260275230, Length=201, Percent_Identity=27.8606965174129, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI260274832, Length=201, Percent_Identity=27.8606965174129, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=34.2307692307692, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=30.0380228136882, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=26.4822134387352, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082183, Length=241, Percent_Identity=24.0663900414938, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=25.6544502617801, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=44.3037974683544, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=31.7647058823529, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25145438, Length=265, Percent_Identity=31.3207547169811, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=28.1124497991968, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=257, Percent_Identity=28.4046692607004, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=26.0377358490566, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=27.1889400921659, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=212, Percent_Identity=28.3018867924528, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=252, Percent_Identity=24.6031746031746, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=37.6923076923077, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=31.8725099601594, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=31.8725099601594, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24653139, Length=278, Percent_Identity=29.4964028776978, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920382, Length=281, Percent_Identity=29.1814946619217, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357171, Length=267, Percent_Identity=26.2172284644195, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24650670, Length=207, Percent_Identity=31.4009661835749, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654903, Length=202, Percent_Identity=25.7425742574257, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29058; Mature: 28927

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEPSAHPVAGAAAPVVTTSRDGGVLTLTLARPEARNSLSEAMMAALTSALAAIAADTA
CCCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VRAVVLAAEGPVYSAGHDLKEIQAHRGDADRGRAYFEDVLKRCATLMTAIVRLPQPVIAA
HHEEEEECCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VEGMATAAGCQLVASCDLAVAGEKARFCTPGVNIGLFCHTPMVALSRNLARKHAMEMLLL
HHHHHHHHHHHHHHHCCEEECCCCCCEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
GEWVEAEEARRMGLVNRVVPAGDALSTAQALARRIAEKSQVPVKLGKAAFYTQMEMGLDD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHH
AYAYASRVMAENMLARDADEGISAVLAKRAPSWEDR
HHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAEPSAHPVAGAAAPVVTTSRDGGVLTLTLARPEARNSLSEAMMAALTSALAAIAADTA
CCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VRAVVLAAEGPVYSAGHDLKEIQAHRGDADRGRAYFEDVLKRCATLMTAIVRLPQPVIAA
HHEEEEECCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VEGMATAAGCQLVASCDLAVAGEKARFCTPGVNIGLFCHTPMVALSRNLARKHAMEMLLL
HHHHHHHHHHHHHHHCCEEECCCCCCEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
GEWVEAEEARRMGLVNRVVPAGDALSTAQALARRIAEKSQVPVKLGKAAFYTQMEMGLDD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHH
AYAYASRVMAENMLARDADEGISAVLAKRAPSWEDR
HHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]