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The map label for this gene is cobS

Identifier: 154248180

GI number: 154248180

Start: 4725912

End: 4726709

Strand: Reverse

Name: cobS

Synonym: Xaut_4260

Alternate gene names: 154248180

Gene position: 4726709-4725912 (Counterclockwise)

Preceding gene: 154248183

Following gene: 154248179

Centisome position: 89.03

GC content: 72.18

Gene sequence:

>798_bases
ATGCAGCTCGATCGGATCGCACAAGACCTCGTCGCCGCCCTGCGCTTCTATTCGCGGCTGCCCCTCCCCGCCGGACGCGA
CGACCCCGACGCCTTCGCCGTGCCCAGCCTCAACCGCATCGCCTATGCCATTCCCCTGGCCGGCGCGGTCATCGGGCTCA
TCGGCGCCGTGGTGCTGGTGGGGGCCCTAGCGCTGAAGCTGCCGGCGTTCCTGGCGTCGGTACTGGCGGTGACCGCACTG
GTGCTCACCACCGGCGCATTCCACGAGGATGGGCTGGCCGACACGGCGGATGGCCTCGGCGGCGGGCGGGACAAGGCCCA
GCGCCTCGCCATCATGCGCGACAGCCGCATCGGTACCTATGGCGGCTGCGCCCTCATCCTGGCCCTGCTGCTGCGGGTGG
CAGCGCTTGAGGCCCTCGTCGCCTCGGCCGGGATGTTCCGCGCCGCGCTGGCGCTGGTGGTGGCGGAGGCGGCCTCGCGG
GCGGCAGGGGTGCTGCTGCTCCTCGCCCTGCCCCCGGCCCGCGCCGATGGCGCCGGTGCCTCGTTCGGACGCCCCTCGGA
ATCTGCGGGACTTGCCTGCGCTCTCGTCGCCGCGCTGCTGGTGGTGGTCATCCTGGTGCCGGGCTTCGGCATAAGCACCG
CCTTTGCCGGCCTCATCGCCCCGCTGGTGGCCCTGTTTGCCATGATGCGGCTGTCGGGCCGGCTGATCGGCGGGCAGACC
GGGGACGTGGCCGGCGCCACCCAGCAGGTGGCGGTGATTGTCTTCCTCCTCGGCGTGCTTATCTTTCCCGGCAGGTGA

Upstream 100 bases:

>100_bases
TGGCGCGGGGGGCGCCCCTTGACCCCGGCCGCAAGACAGGGTCCCAAGGGCGGGCGGCGGGTTCGGCCCGGCGCGCCCCG
CCACGGCCAGCCGGAACGCC

Downstream 100 bases:

>100_bases
GACGGGGCACGAGTCGCCACCGGCTTGCGTTGATCGAACCGAACGAGCGCCGCGCCCTTGACGTCGAGACCAACCGGACA
GATGCCCGAAACGGACGCCA

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MQLDRIAQDLVAALRFYSRLPLPAGRDDPDAFAVPSLNRIAYAIPLAGAVIGLIGAVVLVGALALKLPAFLASVLAVTAL
VLTTGAFHEDGLADTADGLGGGRDKAQRLAIMRDSRIGTYGGCALILALLLRVAALEALVASAGMFRAALALVVAEAASR
AAGVLLLLALPPARADGAGASFGRPSESAGLACALVAALLVVVILVPGFGISTAFAGLIAPLVALFAMMRLSGRLIGGQT
GDVAGATQQVAVIVFLLGVLIFPGR

Sequences:

>Translated_265_residues
MQLDRIAQDLVAALRFYSRLPLPAGRDDPDAFAVPSLNRIAYAIPLAGAVIGLIGAVVLVGALALKLPAFLASVLAVTAL
VLTTGAFHEDGLADTADGLGGGRDKAQRLAIMRDSRIGTYGGCALILALLLRVAALEALVASAGMFRAALALVVAEAASR
AAGVLLLLALPPARADGAGASFGRPSESAGLACALVAALLVVVILVPGFGISTAFAGLIAPLVALFAMMRLSGRLIGGQT
GDVAGATQQVAVIVFLLGVLIFPGR
>Mature_265_residues
MQLDRIAQDLVAALRFYSRLPLPAGRDDPDAFAVPSLNRIAYAIPLAGAVIGLIGAVVLVGALALKLPAFLASVLAVTAL
VLTTGAFHEDGLADTADGLGGGRDKAQRLAIMRDSRIGTYGGCALILALLLRVAALEALVASAGMFRAALALVVAEAASR
AAGVLLLLALPPARADGAGASFGRPSESAGLACALVAALLVVVILVPGFGISTAFAGLIAPLVALFAMMRLSGRLIGGQT
GDVAGATQQVAVIVFLLGVLIFPGR

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=255, Percent_Identity=35.6862745098039, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_XANP2 (A7IB66)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419138.1
- STRING:   A7IB66
- GeneID:   5423169
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4260
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   WADVQLA
- ProtClustDB:   CLSK2516675
- BioCyc:   XAUT78245:XAUT_4260-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26639; Mature: 26639

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1461e138)-; HASH(0x151dc940)-; HASH(0x14e8c060)-; HASH(0x14f1fb30)-; HASH(0x1511c2fc)-; HASH(0x14eb484c)-; HASH(0x149b8854)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDRIAQDLVAALRFYSRLPLPAGRDDPDAFAVPSLNRIAYAIPLAGAVIGLIGAVVLV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GALALKLPAFLASVLAVTALVLTTGAFHEDGLADTADGLGGGRDKAQRLAIMRDSRIGTY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCC
GGCALILALLLRVAALEALVASAGMFRAALALVVAEAASRAAGVLLLLALPPARADGAGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
SFGRPSESAGLACALVAALLVVVILVPGFGISTAFAGLIAPLVALFAMMRLSGRLIGGQT
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GDVAGATQQVAVIVFLLGVLIFPGR
CCCCCHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MQLDRIAQDLVAALRFYSRLPLPAGRDDPDAFAVPSLNRIAYAIPLAGAVIGLIGAVVLV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GALALKLPAFLASVLAVTALVLTTGAFHEDGLADTADGLGGGRDKAQRLAIMRDSRIGTY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCC
GGCALILALLLRVAALEALVASAGMFRAALALVVAEAASRAAGVLLLLALPPARADGAGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
SFGRPSESAGLACALVAALLVVVILVPGFGISTAFAGLIAPLVALFAMMRLSGRLIGGQT
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GDVAGATQQVAVIVFLLGVLIFPGR
CCCCCHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA