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The map label for this gene is ycjG [H]

Identifier: 154248033

GI number: 154248033

Start: 4548549

End: 4549535

Strand: Reverse

Name: ycjG [H]

Synonym: Xaut_4112

Alternate gene names: 154248033

Gene position: 4549535-4548549 (Counterclockwise)

Preceding gene: 154248038

Following gene: 154248027

Centisome position: 85.7

GC content: 73.45

Gene sequence:

>987_bases
ATGGCCCAACTCGCCCTCAGCATCGAGCGATTCCCCATCGCCGGCACCTTCACCATCGCCCGCGGCTCCAAGACGGAAGC
GGTGGTGGTGGAGGCGCGCCTCACCCATAACGGCGCCACCGGGCGCGGCGAATGCGTGCCCTATGCGCGGTACGGCGAGA
CGGTGGAGGGCGTCGCCGCCGCCATCGCGGCCCTCGGCCCCGACATCGCGACGGGCCTCGATCGCGAGGCCCTGCAATAC
CGCCTGCCACCGGGCGCCGCGCGCAATGCCCTCGATTGCGCCTTCTGGGACCTCGAAGCCAAGCTTTCGGGCATGCGGGT
GGAGGAGCGCGCCGGCCTTGCCGCGCTCCACCCCTTGACCACGGCCTTCACCCTGAGCCTCGATACGCCCGAGGCCATGG
CGCAGGCGGCGGCCGCCGCAACGGCGGCCGGGCGGCCGCTACTCAAGCTGAAGCTGGGCGCCCCCGGCGACCGCGCCCGG
CTCCGGGCTATCCGCGCGGCGGCGCCCGAGGCCCGGCTCATCGTGGACGCCAACGAAGGCTGGAGCCCGGCCGACCTGCC
GGTATTGCTGGATACCTGCGCCGCCGTCGGCGTCGAGCTGGTAGAACAGCCCCTGCCCGCCGCCGACGACCGCATGCTGG
CGCGCATCGAGCGCGCGGTGCCGGTCTGCGCCGACGAGAGCGTCCACGACCGCGCTGGCCTTGCCACCCTGCGCGAACGC
TATGACGCGGTGAACATCAAGCTCGACAAGGCCGGCGGCTTCACCGAGGCGCTGGCGCTGGCCGAGGCGGCCGAAGGGCT
CGGCTTCGGCGTGATGGTGGGCTGCATGCTGGCCTCCTCCCTCGCCATGGCCCCTGCCCTGCTGGTGGCGCAGCGGGCGC
AGGTGGTGGACCTCGACGGCCCGCTTCTGCTCGCCCGTGACCGCACGCCGGGGCTGCGATACGAGGGCGCCGTGGTCTAT
CCGCCCGAGCCGGAGCTGTGGGGCTGA

Upstream 100 bases:

>100_bases
ACAAGACGCCTGCGCGTCGAGATCACCAAGGACACACCCCGGCATCATCCGCTCCGGCACCCGCGAAGCGATGGCCGGAA
TCGGATATTAAGCTTAATCC

Downstream 100 bases:

>100_bases
AGCCGGGCGGCGAGGAGCGCGGTGAGCGCCCCCGCCAGCCCGATGGCGCTGGAGACCAGGAAGGCCAGCGGCCCGAAGGC
GGCCCACAGCGGGCCTGACG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAQLALSIERFPIAGTFTIARGSKTEAVVVEARLTHNGATGRGECVPYARYGETVEGVAAAIAALGPDIATGLDREALQY
RLPPGAARNALDCAFWDLEAKLSGMRVEERAGLAALHPLTTAFTLSLDTPEAMAQAAAAATAAGRPLLKLKLGAPGDRAR
LRAIRAAAPEARLIVDANEGWSPADLPVLLDTCAAVGVELVEQPLPAADDRMLARIERAVPVCADESVHDRAGLATLRER
YDAVNIKLDKAGGFTEALALAEAAEGLGFGVMVGCMLASSLAMAPALLVAQRAQVVDLDGPLLLARDRTPGLRYEGAVVY
PPEPELWG

Sequences:

>Translated_328_residues
MAQLALSIERFPIAGTFTIARGSKTEAVVVEARLTHNGATGRGECVPYARYGETVEGVAAAIAALGPDIATGLDREALQY
RLPPGAARNALDCAFWDLEAKLSGMRVEERAGLAALHPLTTAFTLSLDTPEAMAQAAAAATAAGRPLLKLKLGAPGDRAR
LRAIRAAAPEARLIVDANEGWSPADLPVLLDTCAAVGVELVEQPLPAADDRMLARIERAVPVCADESVHDRAGLATLRER
YDAVNIKLDKAGGFTEALALAEAAEGLGFGVMVGCMLASSLAMAPALLVAQRAQVVDLDGPLLLARDRTPGLRYEGAVVY
PPEPELWG
>Mature_327_residues
AQLALSIERFPIAGTFTIARGSKTEAVVVEARLTHNGATGRGECVPYARYGETVEGVAAAIAALGPDIATGLDREALQYR
LPPGAARNALDCAFWDLEAKLSGMRVEERAGLAALHPLTTAFTLSLDTPEAMAQAAAAATAAGRPLLKLKLGAPGDRARL
RAIRAAAPEARLIVDANEGWSPADLPVLLDTCAAVGVELVEQPLPAADDRMLARIERAVPVCADESVHDRAGLATLRERY
DAVNIKLDKAGGFTEALALAEAAEGLGFGVMVGCMLASSLAMAPALLVAQRAQVVDLDGPLLLARDRTPGLRYEGAVVYP
PEPELWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=306, Percent_Identity=47.0588235294118, Blast_Score=255, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34317; Mature: 34186

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLALSIERFPIAGTFTIARGSKTEAVVVEARLTHNGATGRGECVPYARYGETVEGVAA
CCCEEEEHHHCCCEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCHHHCCCHHHHHHH
AIAALGPDIATGLDREALQYRLPPGAARNALDCAFWDLEAKLSGMRVEERAGLAALHPLT
HHHHHCCHHHHCCCHHHHEEECCCCCCCCCCEEEEEEHHHHHCCCEEHHHCCEEHCCCCC
TAFTLSLDTPEAMAQAAAAATAAGRPLLKLKLGAPGDRARLRAIRAAAPEARLIVDANEG
EEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCC
WSPADLPVLLDTCAAVGVELVEQPLPAADDRMLARIERAVPVCADESVHDRAGLATLRER
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YDAVNIKLDKAGGFTEALALAEAAEGLGFGVMVGCMLASSLAMAPALLVAQRAQVVDLDG
CCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
PLLLARDRTPGLRYEGAVVYPPEPELWG
CEEEEECCCCCCEECCEEEECCCCCCCC
>Mature Secondary Structure 
AQLALSIERFPIAGTFTIARGSKTEAVVVEARLTHNGATGRGECVPYARYGETVEGVAA
CCEEEEHHHCCCEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCHHHCCCHHHHHHH
AIAALGPDIATGLDREALQYRLPPGAARNALDCAFWDLEAKLSGMRVEERAGLAALHPLT
HHHHHCCHHHHCCCHHHHEEECCCCCCCCCCEEEEEEHHHHHCCCEEHHHCCEEHCCCCC
TAFTLSLDTPEAMAQAAAAATAAGRPLLKLKLGAPGDRARLRAIRAAAPEARLIVDANEG
EEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCC
WSPADLPVLLDTCAAVGVELVEQPLPAADDRMLARIERAVPVCADESVHDRAGLATLRER
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YDAVNIKLDKAGGFTEALALAEAAEGLGFGVMVGCMLASSLAMAPALLVAQRAQVVDLDG
CCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
PLLLARDRTPGLRYEGAVVYPPEPELWG
CEEEEECCCCCCEECCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]