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The map label for this gene is nac [H]

Identifier: 154247980

GI number: 154247980

Start: 4492266

End: 4493213

Strand: Reverse

Name: nac [H]

Synonym: Xaut_4059

Alternate gene names: 154247980

Gene position: 4493213-4492266 (Counterclockwise)

Preceding gene: 154247987

Following gene: 154247979

Centisome position: 84.63

GC content: 67.51

Gene sequence:

>948_bases
ATGGATCTTCGTCAGCTCAAATACTTCGTCCAGATTGCGGAAAGCGGGAATTTCTCTCGCGCGGCGGAGGTGTTGCGCAT
TGCCCAGCCCTCGCTTAGCCAGCAGATGAAGAATCTGGAGGAAGAGCTGGGCGTCGAGCTGCTCATGCGCCACGCCCGGG
GCGTCACCCCCTCCCAGCTCGGCCAACAATTCTACGACCATGCCCGCCGCATCCTGGAGGAGGTGGACCGGGTGAAGGAC
CAGGTCCGCTCCAAGTCGCTCAATCCCTCGGGGCGGGTGTCGGTGGGGCTGCCCACCTCGGCCTGCCGGGGCCTCGGCCT
GCCGCTGATCTCGGCCATGGCCGAGCACCAGCCCAACATCGCCCTCCATGTGGTGGAGGCCATGACCGGCTATCTCGATG
ATTTCCTCCAGGCCGGCCGGCTGGACGTGGCCCTGCTCTACGACCACAAGGCATTCGAGCATGTGGCCTGGACCGAGATG
ATGGTCGAGGAGCTGATGCTGTTCGTGGCGCCGGACCACCCGCTGGCGGCGCGGGGCAGCGTCTCCTTCCGCGACATGTT
CGCGTGGCCCATCGTGCTGCCCGGCGCGCCCAACGTGATGCGCACGGTGATCGAGCAGTTCGCCGCCCGCAACGATGTGG
AGCCGGTCGCCATGGCCTGCGACAGCCTGCCCACCATCGCCCGCCTGGTGCGCTCCGGCCGGGCGCTGGGGGTGATGCCC
CACTTCGCCTTCATGGATGAGATGGCGCGCGGCGAGATGGTGGCCGTCTCCATCCTCGACCCGACGCCGTCGTGGCGGCT
GTCCGTCGTCGTCTCCCAGCGCAGCATGAACCCGCGCGGCTCCGAGGCGGTGGCCAAGGTGATGGCCAACGTCATCGCCG
AGATGGTCGAGACCGGAACCTGGCGCGCCAAGCTCAAGGCCGAGAAGGCCCGCGCCACCGTGAAGTGA

Upstream 100 bases:

>100_bases
TTATGATTTTAGCGCTCATAGTTTGGGCATATGACTATATATCATGCAATTTGACAATCACTTTTGAGCAATGCCATTTC
AGCTGGTGCAAAGGTGATCC

Downstream 100 bases:

>100_bases
CCCCCGCCAGCCCGTCGAGATGCCCCATGGACCTCATCGTCAAGAACGCCCGCCTGCCGGACGCGCCGGACCGTATCGTC
GATATCGCCATCGCGGCTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MDLRQLKYFVQIAESGNFSRAAEVLRIAQPSLSQQMKNLEEELGVELLMRHARGVTPSQLGQQFYDHARRILEEVDRVKD
QVRSKSLNPSGRVSVGLPTSACRGLGLPLISAMAEHQPNIALHVVEAMTGYLDDFLQAGRLDVALLYDHKAFEHVAWTEM
MVEELMLFVAPDHPLAARGSVSFRDMFAWPIVLPGAPNVMRTVIEQFAARNDVEPVAMACDSLPTIARLVRSGRALGVMP
HFAFMDEMARGEMVAVSILDPTPSWRLSVVVSQRSMNPRGSEAVAKVMANVIAEMVETGTWRAKLKAEKARATVK

Sequences:

>Translated_315_residues
MDLRQLKYFVQIAESGNFSRAAEVLRIAQPSLSQQMKNLEEELGVELLMRHARGVTPSQLGQQFYDHARRILEEVDRVKD
QVRSKSLNPSGRVSVGLPTSACRGLGLPLISAMAEHQPNIALHVVEAMTGYLDDFLQAGRLDVALLYDHKAFEHVAWTEM
MVEELMLFVAPDHPLAARGSVSFRDMFAWPIVLPGAPNVMRTVIEQFAARNDVEPVAMACDSLPTIARLVRSGRALGVMP
HFAFMDEMARGEMVAVSILDPTPSWRLSVVVSQRSMNPRGSEAVAKVMANVIAEMVETGTWRAKLKAEKARATVK
>Mature_315_residues
MDLRQLKYFVQIAESGNFSRAAEVLRIAQPSLSQQMKNLEEELGVELLMRHARGVTPSQLGQQFYDHARRILEEVDRVKD
QVRSKSLNPSGRVSVGLPTSACRGLGLPLISAMAEHQPNIALHVVEAMTGYLDDFLQAGRLDVALLYDHKAFEHVAWTEM
MVEELMLFVAPDHPLAARGSVSFRDMFAWPIVLPGAPNVMRTVIEQFAARNDVEPVAMACDSLPTIARLVRSGRALGVMP
HFAFMDEMARGEMVAVSILDPTPSWRLSVVVSQRSMNPRGSEAVAKVMANVIAEMVETGTWRAKLKAEKARATVK

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=34.6938775510204, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790399, Length=271, Percent_Identity=29.1512915129151, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787601, Length=274, Percent_Identity=24.8175182481752, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=28.5223367697594, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI157672245, Length=239, Percent_Identity=28.4518828451883, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=30.4, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI145693105, Length=274, Percent_Identity=25.1824817518248, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787530, Length=273, Percent_Identity=27.4725274725275, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081904, Length=194, Percent_Identity=29.8969072164948, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367136, Length=144, Percent_Identity=30.5555555555556, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=265, Percent_Identity=24.9056603773585, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789639, Length=143, Percent_Identity=30.0699300699301, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790262, Length=259, Percent_Identity=20.4633204633205, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790208, Length=296, Percent_Identity=22.2972972972973, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789440, Length=120, Percent_Identity=31.6666666666667, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788481, Length=277, Percent_Identity=24.187725631769, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787589, Length=185, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI145693193, Length=119, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788748, Length=250, Percent_Identity=24, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34918; Mature: 34918

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLKYFVQIAESGNFSRAAEVLRIAQPSLSQQMKNLEEELGVELLMRHARGVTPSQL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
GQQFYDHARRILEEVDRVKDQVRSKSLNPSGRVSVGLPTSACRGLGLPLISAMAEHQPNI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHCCHHHHHHHHHCCCCH
ALHVVEAMTGYLDDFLQAGRLDVALLYDHKAFEHVAWTEMMVEELMLFVAPDHPLAARGS
HHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VSFRDMFAWPIVLPGAPNVMRTVIEQFAARNDVEPVAMACDSLPTIARLVRSGRALGVMP
CCHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCEECCH
HFAFMDEMARGEMVAVSILDPTPSWRLSVVVSQRSMNPRGSEAVAKVMANVIAEMVETGT
HHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
WRAKLKAEKARATVK
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLRQLKYFVQIAESGNFSRAAEVLRIAQPSLSQQMKNLEEELGVELLMRHARGVTPSQL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
GQQFYDHARRILEEVDRVKDQVRSKSLNPSGRVSVGLPTSACRGLGLPLISAMAEHQPNI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHCCHHHHHHHHHCCCCH
ALHVVEAMTGYLDDFLQAGRLDVALLYDHKAFEHVAWTEMMVEELMLFVAPDHPLAARGS
HHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VSFRDMFAWPIVLPGAPNVMRTVIEQFAARNDVEPVAMACDSLPTIARLVRSGRALGVMP
CCHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHCCCCEECCH
HFAFMDEMARGEMVAVSILDPTPSWRLSVVVSQRSMNPRGSEAVAKVMANVIAEMVETGT
HHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
WRAKLKAEKARATVK
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]