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The map label for this gene is gatA [H]

Identifier: 154247978

GI number: 154247978

Start: 4489820

End: 4491001

Strand: Reverse

Name: gatA [H]

Synonym: Xaut_4057

Alternate gene names: 154247978

Gene position: 4491001-4489820 (Counterclockwise)

Preceding gene: 154247979

Following gene: 154247973

Centisome position: 84.59

GC content: 74.53

Gene sequence:

>1182_bases
ATGAGCGCCGTCGAGGCGCTGGGCGCCTTCATTCCCGGCCCGCGCCTTGCCCGGCCGGGCGCGCCCGCCGGGCCGCTCGC
CGGCCTCACCTTCGCCGTGAAGGATGTGATGGACGTGGCCGGCACCTCCACCGGCAACGGCCATCCCGACTGGCTCACCA
CCCACCCGCCCGCCGCGCGCTCCGCCGTGGCGGTGGATCGCCTGCTGGATGCGGGGGCAAGCCTTGCCGGCAAGACTATC
GCCGACGAGCTGTGCTACTCGCTCACCGGCGAGAACGTGCATTACGGCACGCCGCTCAACCCGGCCGCGCCGGACCGGGT
GCCCGGCGGCTCCTCCAGCGGCTCGGCCTCGGCCACGGCCGGGAGCGTGGTGGATTTCGCCCTCGGCACCGATTGCGGCG
GCTCCGTGCGGGTGCCGTCGAGCTATTGCGGCCTGTTCGGCATGCGCCCCACCCATGGGCGGATCCCGCTGGAGGGCGTG
GCCCCGTTCGCGCCCAGCTTCGATTGCGTGGGCTGGTTCGCCCGCGACGCCGGGCTTCTGGAACGCGTCGGCCGCGTGCT
TCTGGCCGATGCCGCGCCCGCGCCGCTCCTCACCCGCCTGCTCGCCCCCCGCGAGGCGTTTGCCATGCTCTCGCCGGAGG
CAGCCGCCGCCCTCGCGCCCGCGCTTCGGCTGGCGCAGGAGACAATCGCGCCCGGCGCGGAAATCGCCCTGGCACCCGAT
GGGCTCGATGCCTGGTCCGCCACCTTCCGCACCTTGCAGGCGGCCGAGATCTGGGCCTCGGTGGGGCCGTGGATCGAGGC
GGTGCGCCCCGCCTTCGGACCGGGGGTGAAGGAGCGGTTCGACGCCGCCCGCACCATCTCGCCTTCAGCGGTGGAGGTCG
CTTGCGCCCATCGGGCTGCGATCCGCGCCCGGCTCGACGACCTGCTGCAACCGGGCACGGCTTTCCTGATGCCCACGGTG
CCGCGCCCTGCACCGCTGCGCGGCATGGCCACCGCCCAGGTGGAGGTGGTGACCCGGCATCTGGCCATGAACCTCTTGTG
CATCGCCGGCCTCGGCGGCCTGCCCCAGGTCTCCCTGCCGCTGGCTCGGCTGGAGGGGGTACCGTTCGGCCTCTCGCTGG
TGGGCCCGGCGGGGAGCGACACCGCCCTGCTGAGCGCGGCAGTGGCGCTGGCCGGGGGCTGA

Upstream 100 bases:

>100_bases
TGGCCGAGCTGTCGCCCGTGCTCGCCGCCTTCAAGGGTGGCCGGCGCACCCTGACGCGAGAGCGGGCCAAGCTGCACTTT
CCCGGCTGCGGGTGCGCCCA

Downstream 100 bases:

>100_bases
TCGGACGCCGTGGTTTAAGAGCCCGTTTGAAAATTCGCTCAGATGGGCGCTTGCAGCCCTCCCAAACGCATGATCACCGT
CATGGCCGGGCTTGTCCCGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSAVEALGAFIPGPRLARPGAPAGPLAGLTFAVKDVMDVAGTSTGNGHPDWLTTHPPAARSAVAVDRLLDAGASLAGKTI
ADELCYSLTGENVHYGTPLNPAAPDRVPGGSSSGSASATAGSVVDFALGTDCGGSVRVPSSYCGLFGMRPTHGRIPLEGV
APFAPSFDCVGWFARDAGLLERVGRVLLADAAPAPLLTRLLAPREAFAMLSPEAAAALAPALRLAQETIAPGAEIALAPD
GLDAWSATFRTLQAAEIWASVGPWIEAVRPAFGPGVKERFDAARTISPSAVEVACAHRAAIRARLDDLLQPGTAFLMPTV
PRPAPLRGMATAQVEVVTRHLAMNLLCIAGLGGLPQVSLPLARLEGVPFGLSLVGPAGSDTALLSAAVALAGG

Sequences:

>Translated_393_residues
MSAVEALGAFIPGPRLARPGAPAGPLAGLTFAVKDVMDVAGTSTGNGHPDWLTTHPPAARSAVAVDRLLDAGASLAGKTI
ADELCYSLTGENVHYGTPLNPAAPDRVPGGSSSGSASATAGSVVDFALGTDCGGSVRVPSSYCGLFGMRPTHGRIPLEGV
APFAPSFDCVGWFARDAGLLERVGRVLLADAAPAPLLTRLLAPREAFAMLSPEAAAALAPALRLAQETIAPGAEIALAPD
GLDAWSATFRTLQAAEIWASVGPWIEAVRPAFGPGVKERFDAARTISPSAVEVACAHRAAIRARLDDLLQPGTAFLMPTV
PRPAPLRGMATAQVEVVTRHLAMNLLCIAGLGGLPQVSLPLARLEGVPFGLSLVGPAGSDTALLSAAVALAGG
>Mature_392_residues
SAVEALGAFIPGPRLARPGAPAGPLAGLTFAVKDVMDVAGTSTGNGHPDWLTTHPPAARSAVAVDRLLDAGASLAGKTIA
DELCYSLTGENVHYGTPLNPAAPDRVPGGSSSGSASATAGSVVDFALGTDCGGSVRVPSSYCGLFGMRPTHGRIPLEGVA
PFAPSFDCVGWFARDAGLLERVGRVLLADAAPAPLLTRLLAPREAFAMLSPEAAAALAPALRLAQETIAPGAEIALAPDG
LDAWSATFRTLQAAEIWASVGPWIEAVRPAFGPGVKERFDAARTISPSAVEVACAHRAAIRARLDDLLQPGTAFLMPTVP
RPAPLRGMATAQVEVVTRHLAMNLLCIAGLGGLPQVSLPLARLEGVPFGLSLVGPAGSDTALLSAAVALAGG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=418, Percent_Identity=23.444976076555, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=184, Percent_Identity=35.8695652173913, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=138, Percent_Identity=35.5072463768116, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=143, Percent_Identity=35.6643356643357, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=172, Percent_Identity=31.3953488372093, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=382, Percent_Identity=29.0575916230367, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=392, Percent_Identity=25.2551020408163, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356731, Length=397, Percent_Identity=23.1738035264484, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648113, Length=163, Percent_Identity=38.0368098159509, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644968, Length=352, Percent_Identity=24.1477272727273, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 39823; Mature: 39692

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVEALGAFIPGPRLARPGAPAGPLAGLTFAVKDVMDVAGTSTGNGHPDWLTTHPPAAR
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
SAVAVDRLLDAGASLAGKTIADELCYSLTGENVHYGTPLNPAAPDRVPGGSSSGSASATA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
GSVVDFALGTDCGGSVRVPSSYCGLFGMRPTHGRIPLEGVAPFAPSFDCVGWFARDAGLL
CHHHHHHHCCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ERVGRVLLADAAPAPLLTRLLAPREAFAMLSPEAAAALAPALRLAQETIAPGAEIALAPD
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCC
GLDAWSATFRTLQAAEIWASVGPWIEAVRPAFGPGVKERFDAARTISPSAVEVACAHRAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
IRARLDDLLQPGTAFLMPTVPRPAPLRGMATAQVEVVTRHLAMNLLCIAGLGGLPQVSLP
HHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LARLEGVPFGLSLVGPAGSDTALLSAAVALAGG
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAVEALGAFIPGPRLARPGAPAGPLAGLTFAVKDVMDVAGTSTGNGHPDWLTTHPPAAR
CHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
SAVAVDRLLDAGASLAGKTIADELCYSLTGENVHYGTPLNPAAPDRVPGGSSSGSASATA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
GSVVDFALGTDCGGSVRVPSSYCGLFGMRPTHGRIPLEGVAPFAPSFDCVGWFARDAGLL
CHHHHHHHCCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ERVGRVLLADAAPAPLLTRLLAPREAFAMLSPEAAAALAPALRLAQETIAPGAEIALAPD
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCC
GLDAWSATFRTLQAAEIWASVGPWIEAVRPAFGPGVKERFDAARTISPSAVEVACAHRAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
IRARLDDLLQPGTAFLMPTVPRPAPLRGMATAQVEVVTRHLAMNLLCIAGLGGLPQVSLP
HHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LARLEGVPFGLSLVGPAGSDTALLSAAVALAGG
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA