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The map label for this gene is traG [H]

Identifier: 154247928

GI number: 154247928

Start: 4434900

End: 4436879

Strand: Reverse

Name: traG [H]

Synonym: Xaut_4007

Alternate gene names: 154247928

Gene position: 4436879-4434900 (Counterclockwise)

Preceding gene: 154247929

Following gene: 154247927

Centisome position: 83.57

GC content: 66.21

Gene sequence:

>1980_bases
ATGTCCGCGACCAAGATCCTCTGGGGCCAGATCCTCGCAGTCTTCGCCATCGTGCTCGCCACCACCTGGGGGGCGACGGA
ATATGTCGCCTGGCGGCTGGGCTTCCAGGACCAACTCGGCTCACCCTGGTTCGTGCTGGGGTACTGGCCCGTCTATCACC
CGCCGGCCTTCTTCTGGTGGTGGTATTTCTACGATGCCTATGCGCCGGAGATTTTCACAGAAGGCGCCTTCATCGCGGCC
TCGGGCGGGTTCCTGTCCATTGCGATCGCCATCGGCCTGTCGGTCTGGCGGGCCCGGGAGGCGAAGCGGGTCGAGACCTA
CGGTTCCGCCCGCTGGGCCGAGCCGGACGAGATCCGTTCAGCCGGTCTTCTCGGATCCGACGGGGTAGTGCTCGGCCGCC
ATGGAGGGGACTATCTGCGCCATGACGGGCCCGAGCACGTCCTGTGCTTCGCGCCCACCCGGTCGGGCAAGGGCGTGGGC
CTCGTCGTCCCATCGCTCCTCACCTGGCCAGGCTCTGCCATCGTCCACGACATCAAGGGCGAAAACTGGCAGCTCACCGC
CGGCTTCCGGGCACGGCACGGGCGCGTGTTGCTGTTCGACCCCACCAATGTGAAATCGGCCGCTTACAATCCGCTGCTGG
AGGTCCGCCGCGGGGAGTGGGAGGTCCGCGACGTCCAGAACATCGCCGATATCCTGGTCGACCCTGAGGGCTCACTGGAA
AAGCGCAACCACTGGGAAAAGACCAGCCACGCTCTGCTCGTCGGCGCCATTCTCCATGTGCTCTATGCCGAGCGCGACAA
GACCCTCGCCGGCGTCGCCGTCTTCCTCTCCGACCCCAGGCGCCCCATCGAGGCGACACTCGCCGCCATGATGACGACGC
CGCACCTCGGCGAGACGGGTCCTCATCCGGTGATCGCCAGCGCGGCACGGGAGCTTCTCAATAAGTCGGAGAACGAGCGC
TCCGGCGTTCTTTCCACCGCCATGTCCTTCCTCGGGCTCTATCGCGATCCGGTGGTCGCCAAGGTGACCCGGCGCTGCGA
TTGGCGCATCGCCGACCTTGTAGCGCACCGGCAGCCGACCACCCTCTATCTCGTCGTGCCGCCGTCCGACATCAACCGCA
CCAAGCCTCTGATCCGCCTGATCCTGAACCAGGTCGGGCGTCGCCTCACCGAGGACCTGCAGGCGAAGGCCGGCCGCCAT
CGGCTCCTCCTGATGCTGGACGAGTTTCCGGCGCTGGGGCGGCTCGACTTCTTCGAGAGCGCCCTCGCATTCATGGCGGG
CTACGAGCTCAAGAGCTTCCTGATCGCGCAGAGCCTGAACCAGATCGAGAAGGCCTATGGCCCGAACAATTCGATCCTCG
ACAACTGCCATGTGCGGGTGAGCTTTGCCACCAATGACGAGCGCACCGCCAAGCGTGTCTCCGACGCCCTCGGCACCGCC
ACCGAGATGAAGGCCATGAAGAACTATGCCGGCCACCGGCTCTCGCCCTGGCTCGGCCACCTGATGGTGTCGCGCTCGGA
GACGGCCCGCCCGCTCCTCACGCCGGGTGAGGTGATGCAGCTTCCACCCGCCGACGAGATCGTCATGGTCGCCGGCACGC
CGCCGATCCGGGCGAAGAAGGCGCGGTACTATGAGGATGCGCGCTTCCGGGAGCGAATCCTGCAGCCACCTCTGCTTGCA
GAACCCAACGAGCAGCGCGCCGACGACTGGACCGTGTTGCCGTTGCCCGCACCCTCGCAACTGAGCCTGATGGTGCAGGG
AAGGGATGATGAGGACACGACCGGGTCGGAGCGCCGACACCAGCCGGAGCTGGCTCGGGTGGCTTCGGTCGAGGAGCTTG
CATTACCGCAGAACGAGTTCGAGATCGACCTCGCGGACGAGAGTGAGGACGACGTCGCCCGAAACAGCCGCATGACGCGC
GTCATGCAGGGTGTGGCGCGTCAGGTCTCGCTCGACCCCCGCGACGGCATGGAGCTGTGA

Upstream 100 bases:

>100_bases
GCACGTCTCCGGTGTCACCCGGTCCGATGGTGGCATCGACTGGTCCTTCGGCCGCAAGCGCGGCCTCGGCCTCTGACCTC
AAGCGCAAAAGGACCGCCGC

Downstream 100 bases:

>100_bases
CGGCGATGGCTCCGCGCAAAAAGAAGGCGCAGATCACTGTCTATCTCGAACCGGACACCATGGCCTTGCTCGCTGATTAC
GCTGCGCGCCGGGACCAGCC

Product: conjugal transfer coupling protein TraG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MSATKILWGQILAVFAIVLATTWGATEYVAWRLGFQDQLGSPWFVLGYWPVYHPPAFFWWWYFYDAYAPEIFTEGAFIAA
SGGFLSIAIAIGLSVWRAREAKRVETYGSARWAEPDEIRSAGLLGSDGVVLGRHGGDYLRHDGPEHVLCFAPTRSGKGVG
LVVPSLLTWPGSAIVHDIKGENWQLTAGFRARHGRVLLFDPTNVKSAAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLE
KRNHWEKTSHALLVGAILHVLYAERDKTLAGVAVFLSDPRRPIEATLAAMMTTPHLGETGPHPVIASAARELLNKSENER
SGVLSTAMSFLGLYRDPVVAKVTRRCDWRIADLVAHRQPTTLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRH
RLLLMLDEFPALGRLDFFESALAFMAGYELKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTA
TEMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKKARYYEDARFRERILQPPLLA
EPNEQRADDWTVLPLPAPSQLSLMVQGRDDEDTTGSERRHQPELARVASVEELALPQNEFEIDLADESEDDVARNSRMTR
VMQGVARQVSLDPRDGMEL

Sequences:

>Translated_659_residues
MSATKILWGQILAVFAIVLATTWGATEYVAWRLGFQDQLGSPWFVLGYWPVYHPPAFFWWWYFYDAYAPEIFTEGAFIAA
SGGFLSIAIAIGLSVWRAREAKRVETYGSARWAEPDEIRSAGLLGSDGVVLGRHGGDYLRHDGPEHVLCFAPTRSGKGVG
LVVPSLLTWPGSAIVHDIKGENWQLTAGFRARHGRVLLFDPTNVKSAAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLE
KRNHWEKTSHALLVGAILHVLYAERDKTLAGVAVFLSDPRRPIEATLAAMMTTPHLGETGPHPVIASAARELLNKSENER
SGVLSTAMSFLGLYRDPVVAKVTRRCDWRIADLVAHRQPTTLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRH
RLLLMLDEFPALGRLDFFESALAFMAGYELKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTA
TEMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKKARYYEDARFRERILQPPLLA
EPNEQRADDWTVLPLPAPSQLSLMVQGRDDEDTTGSERRHQPELARVASVEELALPQNEFEIDLADESEDDVARNSRMTR
VMQGVARQVSLDPRDGMEL
>Mature_658_residues
SATKILWGQILAVFAIVLATTWGATEYVAWRLGFQDQLGSPWFVLGYWPVYHPPAFFWWWYFYDAYAPEIFTEGAFIAAS
GGFLSIAIAIGLSVWRAREAKRVETYGSARWAEPDEIRSAGLLGSDGVVLGRHGGDYLRHDGPEHVLCFAPTRSGKGVGL
VVPSLLTWPGSAIVHDIKGENWQLTAGFRARHGRVLLFDPTNVKSAAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLEK
RNHWEKTSHALLVGAILHVLYAERDKTLAGVAVFLSDPRRPIEATLAAMMTTPHLGETGPHPVIASAARELLNKSENERS
GVLSTAMSFLGLYRDPVVAKVTRRCDWRIADLVAHRQPTTLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRHR
LLLMLDEFPALGRLDFFESALAFMAGYELKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTAT
EMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKKARYYEDARFRERILQPPLLAE
PNEQRADDWTVLPLPAPSQLSLMVQGRDDEDTTGSERRHQPELARVASVEELALPQNEFEIDLADESEDDVARNSRMTRV
MQGVARQVSLDPRDGMEL

Specific function: Required for conjugative transfer of plasmid RP4. Binds tightly and specifically to the relaxase traI. Can also bind to DNA without sequence specificity. May form a pore-like structure that could serve as a channel for DNA transfer [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virD4/traG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003688 [H]

Pfam domain/function: PF02534 TraG [H]

EC number: NA

Molecular weight: Translated: 73557; Mature: 73426

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATKILWGQILAVFAIVLATTWGATEYVAWRLGFQDQLGSPWFVLGYWPVYHPPAFFWW
CCCCHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCHHHCCCCEEEEEECCCCCCCHHHHH
WYFYDAYAPEIFTEGAFIAASGGFLSIAIAIGLSVWRAREAKRVETYGSARWAEPDEIRS
HHHHHHHCHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AGLLGSDGVVLGRHGGDYLRHDGPEHVLCFAPTRSGKGVGLVVPSLLTWPGSAIVHDIKG
CCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEECCHHHCCCCCCEEEECCC
ENWQLTAGFRARHGRVLLFDPTNVKSAAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLE
CCEEEEECCCCCCCEEEEECCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHEECCCCCCH
KRNHWEKTSHALLVGAILHVLYAERDKTLAGVAVFLSDPRRPIEATLAAMMTTPHLGETG
HHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCC
PHPVIASAARELLNKSENERSGVLSTAMSFLGLYRDPVVAKVTRRCDWRIADLVAHRQPT
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCC
TLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRHRLLLMLDEFPALGRLDFFES
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHH
ALAFMAGYELKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCH
TEMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKK
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHH
ARYYEDARFRERILQPPLLAEPNEQRADDWTVLPLPAPSQLSLMVQGRDDEDTTGSERRH
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCHHCC
QPELARVASVEELALPQNEFEIDLADESEDDVARNSRMTRVMQGVARQVSLDPRDGMEL
CCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SATKILWGQILAVFAIVLATTWGATEYVAWRLGFQDQLGSPWFVLGYWPVYHPPAFFWW
CCCHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCHHHCCCCEEEEEECCCCCCCHHHHH
WYFYDAYAPEIFTEGAFIAASGGFLSIAIAIGLSVWRAREAKRVETYGSARWAEPDEIRS
HHHHHHHCHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AGLLGSDGVVLGRHGGDYLRHDGPEHVLCFAPTRSGKGVGLVVPSLLTWPGSAIVHDIKG
CCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEECCHHHCCCCCCEEEECCC
ENWQLTAGFRARHGRVLLFDPTNVKSAAYNPLLEVRRGEWEVRDVQNIADILVDPEGSLE
CCEEEEECCCCCCCEEEEECCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHEECCCCCCH
KRNHWEKTSHALLVGAILHVLYAERDKTLAGVAVFLSDPRRPIEATLAAMMTTPHLGETG
HHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCC
PHPVIASAARELLNKSENERSGVLSTAMSFLGLYRDPVVAKVTRRCDWRIADLVAHRQPT
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCC
TLYLVVPPSDINRTKPLIRLILNQVGRRLTEDLQAKAGRHRLLLMLDEFPALGRLDFFES
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHH
ALAFMAGYELKSFLIAQSLNQIEKAYGPNNSILDNCHVRVSFATNDERTAKRVSDALGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCH
TEMKAMKNYAGHRLSPWLGHLMVSRSETARPLLTPGEVMQLPPADEIVMVAGTPPIRAKK
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCHHH
ARYYEDARFRERILQPPLLAEPNEQRADDWTVLPLPAPSQLSLMVQGRDDEDTTGSERRH
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCHHCC
QPELARVASVEELALPQNEFEIDLADESEDDVARNSRMTRVMQGVARQVSLDPRDGMEL
CCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1665997; 8021214; 11976307 [H]