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The map label for this gene is gne [H]

Identifier: 154247488

GI number: 154247488

Start: 3976104

End: 3977063

Strand: Direct

Name: gne [H]

Synonym: Xaut_3561

Alternate gene names: 154247488

Gene position: 3976104-3977063 (Clockwise)

Preceding gene: 154247487

Following gene: 154247489

Centisome position: 74.89

GC content: 60.62

Gene sequence:

>960_bases
ATGAGTGGAAAGATTATCGTTTTCGGCGGTTCTGGATTTGTCGGCACCCATCTTATTCGATCGCTCGTAGCGCAGGGCGA
GCAGATCGTCAGCGTGGACATCCGTCCGCAGCGCGAAACCCTTCCGCAGGTGGACTATCGGATCGGAGACGTCCGGGACC
TGTCGGCCTTCGAGGTCGATGGCCCGGTCGCGACGATCTACAATTTCGCCGCGGTCCACACCACGCCCGGCCATCCCTTC
TGGGAGTATTACGACACCAACATCAATGGCGCGATCGAGATCACCGCCTGGGCGCGGCGCTATGGTGTGAACGACATCGT
GTTCACCAGCTCCATCTCGGTCTACGGCCCGTCGGAAGAGACCAAGTCGGAAGTCTCCACACCGGCGCCGCAGTCTGCCT
ACGGCTATTCCAAGCTGATGGCCGAGCGCATTCACCGCATCTGGTTCGACGAGGCGCCGGGCCGGCGGCTGACCGTGGTG
CGTCCGGCGGTGGTGTTCGGCCGCGGCGAGAGGGGCAACTTTACCCGCCTCGCGCGGCTGCTCCAGAAGGGCTTCTTTGT
CTATCCGGGGCGCCGCGACACCATCAAGGCCTGCGTCTATGTGGAAGACCTGCTGGATTCCATCGCCTTCGCCAAAAACA
GCCCCGAACCGTTCAAGTTCTACAACGCGGCCTATCCGGAGCGTTACACGCTGGAGCAGGTAGTCTCGCTCTTCATCGCG
GACCACTTTCCCAATGGGAAGACCTACACAATTCCCCGCGGCGTCATGATGACGGGCGCCTCGATCCTTGAGAAAACCAG
CTTTCTCAAGATCGGCATTCACCCCGAACGGGTGATGAAGCTGGTGCGCTCAACCGATATTTTCCCGGGGTGGCTCACAT
CGAAAGGATACGTGTTTCCTTACAATCTCAAGGCGGGCCTCCAGCGCTGGTCCGACCAGGGCAACAAGGCCTTCGACTGA

Upstream 100 bases:

>100_bases
GTGACATGGCTGCCTGAGGCAGAGTTAGGCGCGGTTCGATGCGGCGGCCGGCACGTTGGGGTCGCAAAACCACCGGGTTC
CGGGGGTGCAGGAGATTGAG

Downstream 100 bases:

>100_bases
GCGGGGGGGGATCCGGCCGGAGCGGAGAGGTCCCGAACGAGCCTCTAAATTTGTTTGGTGGGGATCGCCGGCTGGATTCG
GGCTTTGCATTCAAAGTTGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: UDP-GlcNAc 4-epimerase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSGKIIVFGGSGFVGTHLIRSLVAQGEQIVSVDIRPQRETLPQVDYRIGDVRDLSAFEVDGPVATIYNFAAVHTTPGHPF
WEYYDTNINGAIEITAWARRYGVNDIVFTSSISVYGPSEETKSEVSTPAPQSAYGYSKLMAERIHRIWFDEAPGRRLTVV
RPAVVFGRGERGNFTRLARLLQKGFFVYPGRRDTIKACVYVEDLLDSIAFAKNSPEPFKFYNAAYPERYTLEQVVSLFIA
DHFPNGKTYTIPRGVMMTGASILEKTSFLKIGIHPERVMKLVRSTDIFPGWLTSKGYVFPYNLKAGLQRWSDQGNKAFD

Sequences:

>Translated_319_residues
MSGKIIVFGGSGFVGTHLIRSLVAQGEQIVSVDIRPQRETLPQVDYRIGDVRDLSAFEVDGPVATIYNFAAVHTTPGHPF
WEYYDTNINGAIEITAWARRYGVNDIVFTSSISVYGPSEETKSEVSTPAPQSAYGYSKLMAERIHRIWFDEAPGRRLTVV
RPAVVFGRGERGNFTRLARLLQKGFFVYPGRRDTIKACVYVEDLLDSIAFAKNSPEPFKFYNAAYPERYTLEQVVSLFIA
DHFPNGKTYTIPRGVMMTGASILEKTSFLKIGIHPERVMKLVRSTDIFPGWLTSKGYVFPYNLKAGLQRWSDQGNKAFD
>Mature_318_residues
SGKIIVFGGSGFVGTHLIRSLVAQGEQIVSVDIRPQRETLPQVDYRIGDVRDLSAFEVDGPVATIYNFAAVHTTPGHPFW
EYYDTNINGAIEITAWARRYGVNDIVFTSSISVYGPSEETKSEVSTPAPQSAYGYSKLMAERIHRIWFDEAPGRRLTVVR
PAVVFGRGERGNFTRLARLLQKGFFVYPGRRDTIKACVYVEDLLDSIAFAKNSPEPFKFYNAAYPERYTLEQVVSLFIAD
HFPNGKTYTIPRGVMMTGASILEKTSFLKIGIHPERVMKLVRSTDIFPGWLTSKGYVFPYNLKAGLQRWSDQGNKAFD

Specific function: Converts UDP-GlcNAc to UDP-GalNAc. Essential for O antigen synthesis [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Escherichia coli, GI1786974, Length=218, Percent_Identity=28.4403669724771, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.7 [H]

Molecular weight: Translated: 35875; Mature: 35744

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKIIVFGGSGFVGTHLIRSLVAQGEQIVSVDIRPQRETLPQVDYRIGDVRDLSAFEVD
CCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHCCCCHHCCCCCCCCCCEEECC
GPVATIYNFAAVHTTPGHPFWEYYDTNINGAIEITAWARRYGVNDIVFTSSISVYGPSEE
CCHHHHEEEEEEECCCCCCCCEEEECCCCCEEEEEEHHHHCCCCEEEEEEEEEEECCCHH
TKSEVSTPAPQSAYGYSKLMAERIHRIWFDEAPGRRLTVVRPAVVFGRGERGNFTRLARL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEECCCCCCHHHHHHH
LQKGFFVYPGRRDTIKACVYVEDLLDSIAFAKNSPEPFKFYNAAYPERYTLEQVVSLFIA
HHCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
DHFPNGKTYTIPRGVMMTGASILEKTSFLKIGIHPERVMKLVRSTDIFPGWLTSKGYVFP
HCCCCCCEEEECCCHHHHCHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEE
YNLKAGLQRWSDQGNKAFD
ECHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGKIIVFGGSGFVGTHLIRSLVAQGEQIVSVDIRPQRETLPQVDYRIGDVRDLSAFEVD
CCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHCCCCHHCCCCCCCCCCEEECC
GPVATIYNFAAVHTTPGHPFWEYYDTNINGAIEITAWARRYGVNDIVFTSSISVYGPSEE
CCHHHHEEEEEEECCCCCCCCEEEECCCCCEEEEEEHHHHCCCCEEEEEEEEEEECCCHH
TKSEVSTPAPQSAYGYSKLMAERIHRIWFDEAPGRRLTVVRPAVVFGRGERGNFTRLARL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEECCCCCCHHHHHHH
LQKGFFVYPGRRDTIKACVYVEDLLDSIAFAKNSPEPFKFYNAAYPERYTLEQVVSLFIA
HHCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
DHFPNGKTYTIPRGVMMTGASILEKTSFLKIGIHPERVMKLVRSTDIFPGWLTSKGYVFP
HCCCCCCEEEECCCHHHHCHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEE
YNLKAGLQRWSDQGNKAFD
ECHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976290; 11206551; 11258796 [H]