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The map label for this gene is irlS [H]

Identifier: 154247446

GI number: 154247446

Start: 3919603

End: 3920991

Strand: Direct

Name: irlS [H]

Synonym: Xaut_3518

Alternate gene names: 154247446

Gene position: 3919603-3920991 (Clockwise)

Preceding gene: 154247445

Following gene: 154247447

Centisome position: 73.83

GC content: 68.32

Gene sequence:

>1389_bases
ATGCGCTTTTTCGCCCACCTCGCGCGGCTGGTGCGCACCACGGCGTTCAAGCTGCTGGCGGCCTATCTGCTGGTGTTCGC
CGTGTTCGCGGTGTCGGTCATCGTCTACACGGCGTGGCACACCCGCGAGCTGATCCAGGGACAGGTGGCGGACGATCTCG
ACCGCGAGGTGCGCTTCCTCGCCGAGCAATACCGTCTCGGCGGCATCCAGCGCCTGGTCTATGTCATCGACCGGCGGACG
CGCCGGCCGGGCTCGTCCATCTACATGATCACCAATTTCCAGGGCGAGGTGCTGGCCACCAACGTTTCGGACCTGCCCAT
CGGCCTCCTCGACAAGGACGGCACCCGCTTCACCACCTACAATCGTCCCGACGATGCCGGTCTGAAGAGCCATGTGGCCT
TCGTGCAGGCCATGATCCTGCCCGGCGGCTACCGCCTGCTGGTTGGCCGCGACATCGAGGAGCGCGACACCCTGCGCGAC
CTCGTGGCGCGACCGGCGCAATGGGCGGTGATGCTGATCGTGGTGCTGGGGCTTGCCGGCGGCGTTTTCGTGACGCGGCG
GGTGTTGAAACGCATCGATTCCATGACGGCCACCGCCGAGACCATCATGGCCGGCAACCTCTCCGGCCGCCTCGCCATGA
CCGGCACCCAGGACGAGTTTGACCGCCTAGCCCATTCGCTGAACGCCATGCTGGACCGCATCGAGGGCCTGATGCAGGGC
CTGAAAGAGGTCTCGGACAATATCGCCCACGACCTGAAGACCCCGCTCACCCGCCTGCGCAACCGCTCGGAGGAGGCGCT
GCGCACCGCCCATACGGAGGACGAGTGGCGCGCGGCGCTGGAAGACACCATCGAGGAGAGCGATGGCCTCATCCGCACCT
TCGACGCGCTGCTCATGATCGCCCGCGCCGAGGCCGGGCAGGCCCGCGCCATCATGAGCGATCTCGACCTCGCAGAGATC
GCCGAGAACGTCTCCGAGCTCTATGAACCGCTGGCCGACGAGCAGGGCCTCGACCTTGTGGTCACGGTGGCGCCGGTGAG
CATTCACGGCGTGAAGGAACTGCTGGCGCAGGCGGTCTCGAACCTCATCGACAATGCCATCAAGTATGGCCGCCCGGTGG
ACGGGAGCAGGGGGCGCATCGCCGTGACCCTGGCGCGGCAGGGCGGGGAAGCGGTGCTCGCGGTGGCGGACACCGGCCCC
GGCATCCCGGAGGCGGATCGCGAGCGGGTGGTGGAGCGCTTCGTGCGGCTGGAGGCGAGCCGCACGCGGCCGGGTTCCGG
GCTGGGCCTGGCGCTGGTGGGCGCGGTGGCGCGCCTGCATGGCGGCAGCCTGAGCTTCACCGATGGAGCGCCGGGGCTGG
TCGCGACGCTGCGTTTACCGGCGAAATAA

Upstream 100 bases:

>100_bases
CACACCGTGCGCGGCGCCGGCTACATGGTGCGGGCGGGTGCCGCTTGAGTCCGGCGGGCGCGGGCCGCTCGCCGTCGGCG
GCGTGATCTGGCGGGTTCTC

Downstream 100 bases:

>100_bases
TTGGGCGCCGGGACCGGCTGTCCCACGCTGGGCATTAAGGCCTTCGCAGGTGCGATAATTTGCTGTTTATCATGGAGCTT
TCGACCGCACATCAGCGTTG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRFFAHLARLVRTTAFKLLAAYLLVFAVFAVSVIVYTAWHTRELIQGQVADDLDREVRFLAEQYRLGGIQRLVYVIDRRT
RRPGSSIYMITNFQGEVLATNVSDLPIGLLDKDGTRFTTYNRPDDAGLKSHVAFVQAMILPGGYRLLVGRDIEERDTLRD
LVARPAQWAVMLIVVLGLAGGVFVTRRVLKRIDSMTATAETIMAGNLSGRLAMTGTQDEFDRLAHSLNAMLDRIEGLMQG
LKEVSDNIAHDLKTPLTRLRNRSEEALRTAHTEDEWRAALEDTIEESDGLIRTFDALLMIARAEAGQARAIMSDLDLAEI
AENVSELYEPLADEQGLDLVVTVAPVSIHGVKELLAQAVSNLIDNAIKYGRPVDGSRGRIAVTLARQGGEAVLAVADTGP
GIPEADRERVVERFVRLEASRTRPGSGLGLALVGAVARLHGGSLSFTDGAPGLVATLRLPAK

Sequences:

>Translated_462_residues
MRFFAHLARLVRTTAFKLLAAYLLVFAVFAVSVIVYTAWHTRELIQGQVADDLDREVRFLAEQYRLGGIQRLVYVIDRRT
RRPGSSIYMITNFQGEVLATNVSDLPIGLLDKDGTRFTTYNRPDDAGLKSHVAFVQAMILPGGYRLLVGRDIEERDTLRD
LVARPAQWAVMLIVVLGLAGGVFVTRRVLKRIDSMTATAETIMAGNLSGRLAMTGTQDEFDRLAHSLNAMLDRIEGLMQG
LKEVSDNIAHDLKTPLTRLRNRSEEALRTAHTEDEWRAALEDTIEESDGLIRTFDALLMIARAEAGQARAIMSDLDLAEI
AENVSELYEPLADEQGLDLVVTVAPVSIHGVKELLAQAVSNLIDNAIKYGRPVDGSRGRIAVTLARQGGEAVLAVADTGP
GIPEADRERVVERFVRLEASRTRPGSGLGLALVGAVARLHGGSLSFTDGAPGLVATLRLPAK
>Mature_462_residues
MRFFAHLARLVRTTAFKLLAAYLLVFAVFAVSVIVYTAWHTRELIQGQVADDLDREVRFLAEQYRLGGIQRLVYVIDRRT
RRPGSSIYMITNFQGEVLATNVSDLPIGLLDKDGTRFTTYNRPDDAGLKSHVAFVQAMILPGGYRLLVGRDIEERDTLRD
LVARPAQWAVMLIVVLGLAGGVFVTRRVLKRIDSMTATAETIMAGNLSGRLAMTGTQDEFDRLAHSLNAMLDRIEGLMQG
LKEVSDNIAHDLKTPLTRLRNRSEEALRTAHTEDEWRAALEDTIEESDGLIRTFDALLMIARAEAGQARAIMSDLDLAEI
AENVSELYEPLADEQGLDLVVTVAPVSIHGVKELLAQAVSNLIDNAIKYGRPVDGSRGRIAVTLARQGGEAVLAVADTGP
GIPEADRERVVERFVRLEASRTRPGSGLGLALVGAVARLHGGSLSFTDGAPGLVATLRLPAK

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=28.7162162162162, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788279, Length=277, Percent_Identity=27.4368231046931, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787894, Length=292, Percent_Identity=30.4794520547945, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790861, Length=431, Percent_Identity=27.8422273781903, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=27.4725274725275, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=25.4416961130742, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789403, Length=329, Percent_Identity=27.9635258358663, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789808, Length=220, Percent_Identity=31.3636363636364, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=225, Percent_Identity=28, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790551, Length=277, Percent_Identity=27.4368231046931, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786600, Length=205, Percent_Identity=28.780487804878, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789149, Length=330, Percent_Identity=25.1515151515152, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=25.7028112449799, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50529; Mature: 50529

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFAHLARLVRTTAFKLLAAYLLVFAVFAVSVIVYTAWHTRELIQGQVADDLDREVRFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AEQYRLGGIQRLVYVIDRRTRRPGSSIYMITNFQGEVLATNVSDLPIGLLDKDGTRFTTY
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCEEECC
NRPDDAGLKSHVAFVQAMILPGGYRLLVGRDIEERDTLRDLVARPAQWAVMLIVVLGLAG
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GVFVTRRVLKRIDSMTATAETIMAGNLSGRLAMTGTQDEFDRLAHSLNAMLDRIEGLMQG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LKEVSDNIAHDLKTPLTRLRNRSEEALRTAHTEDEWRAALEDTIEESDGLIRTFDALLMI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARAEAGQARAIMSDLDLAEIAENVSELYEPLADEQGLDLVVTVAPVSIHGVKELLAQAVS
HHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
NLIDNAIKYGRPVDGSRGRIAVTLARQGGEAVLAVADTGPGIPEADRERVVERFVRLEAS
HHHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHC
RTRPGSGLGLALVGAVARLHGGSLSFTDGAPGLVATLRLPAK
CCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCC
>Mature Secondary Structure
MRFFAHLARLVRTTAFKLLAAYLLVFAVFAVSVIVYTAWHTRELIQGQVADDLDREVRFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AEQYRLGGIQRLVYVIDRRTRRPGSSIYMITNFQGEVLATNVSDLPIGLLDKDGTRFTTY
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCEEECC
NRPDDAGLKSHVAFVQAMILPGGYRLLVGRDIEERDTLRDLVARPAQWAVMLIVVLGLAG
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GVFVTRRVLKRIDSMTATAETIMAGNLSGRLAMTGTQDEFDRLAHSLNAMLDRIEGLMQG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LKEVSDNIAHDLKTPLTRLRNRSEEALRTAHTEDEWRAALEDTIEESDGLIRTFDALLMI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ARAEAGQARAIMSDLDLAEIAENVSELYEPLADEQGLDLVVTVAPVSIHGVKELLAQAVS
HHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
NLIDNAIKYGRPVDGSRGRIAVTLARQGGEAVLAVADTGPGIPEADRERVVERFVRLEAS
HHHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHC
RTRPGSGLGLALVGAVARLHGGSLSFTDGAPGLVATLRLPAK
CCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]