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The map label for this gene is nuoF2 [H]

Identifier: 154247408

GI number: 154247408

Start: 3875145

End: 3876707

Strand: Reverse

Name: nuoF2 [H]

Synonym: Xaut_3480

Alternate gene names: 154247408

Gene position: 3876707-3875145 (Counterclockwise)

Preceding gene: 154247409

Following gene: 154247407

Centisome position: 73.02

GC content: 68.07

Gene sequence:

>1563_bases
ATGAAATCCCCCCGCATCTTCGTCCCGAACGACGCCACTGCCGTCGCCTGCGGCGCCGACCGCGTCGCGCGCAAGCTGGA
AGCCGGGCTGCTGGCCCGCGGCATCACTGCCGACATCACCCGCAACGGCTCGCGCGGCCTGTTCTGGCTGGAACCCCTCA
TCGAGGTTGAGACCGAGCGCGGCCGCATCGGCTACGGCCCGGTGAAGCCCTCGGATATCGAGGACCTGCTTCACGCCGGC
ATCCTCCATGGCGCGGACCACCCCCTGTGCGTCGGCTTGGTGGACGAGATCCCGTACCTGAAGAAGCAGACGCGGCTCAC
CTTCGCCCGCATCGGCATCATCGATCCGCTGTCGCTGACCGATTACAAGGCCCACGGCGGCTATCGCGGGCTGGAGCGCG
CCATCACGCTCGGCCCGTCGGCCACGGTGGAAGAGGTGGTGGAAAGCGGCCTGCGCGGGCGCGGCGGCGCGGGCTTTCCC
ACCGGGATCAAGTGGCGCACGGTGGCCGCTGCCCCGGCCGACCAGCGCTACATCGTCTGCAACGCGGACGAGGGCGACTC
GGGCACCTTCGCCGACCGTCTGATCATGGAGGCGGACCCGTTCTGCCTCATCGAAGGCATGACCATCGCCGGTCTCGCGG
TCGGGGCGACCAAGGGCTACGTCTACTCGCGCTCGGAATATCCCCTCGCCTTCGTGGTGATGGAAAAGGCCATCGAGAAG
GCGCGCGAGGCGGGCCTTTTGGGCGACAACATCCTCGGCTCGGGCCTGTCGTTCGACATGGAAATGCGCATGGGCGCCGG
CGCCTATGTGTGCGGCGAGGAAACCGCCCTCTTGGAAAGCCTCGAGGGCAAGCGCGGCCTGGTGCGCGCGAAGCCGCCGC
TGCCGGCCCACAAGGGCCTGTTCCAGAAGCCCACCGTCATCAACAACCTGGTGTCGCTGGCCACCGTGCCGGTGATCCTG
GACAAGGGCGGCAAGTATTACCGCGACTTCGGCATGGGCCGCTCGCGCGGCACCATGCCGATCCAGATCGCCGGCAACAT
CAAGTTCGGCGGCCTGTTCGAGACCGCCTTCGGCATCCCGCTGGGAGAGCTGGTCTATGACATCGGCGGCGGCACGGCCT
CCGGCCGTCCGGTGAAGGCGGTGCAGGCCGGCGGGCCGCTGGGCGCCTACCTGCCCACCCACCAGTTCGACACCCCGTTC
GACTACGAGGCCTTCGCCGCCAAGGACGCGCTCATCGGCCACGGTGGCCTCGTGGTGTTCGACGACACGGCGGACCTCGC
CGACATGGCGAAGTTCGCCATGGAATTCTGCGCCCATGAGAGCTGCGGCAAGTGCACGCCCTGCCGCATCGGCTCCACCC
GGGGCGTCGAGACCATCGACAAGGTGATCGCCGGCGAGAAGCGCGAGCAGAATCTCGCCCTGCTCACGGACCTTTGCCAC
ACCATGAAGCTTGGTTCCCTGTGCGCGCTGGGCGGCTTCACGCCCTACCCGGTGCTGAGCGCGCTCAATCATTTCCCGGA
AGACTTCGGCGTCGCGCCGAAGCTGCCGGCCGCCGCAGAATAA

Upstream 100 bases:

>100_bases
CCGCTCTGGTGGACGGCCAGCTGGTGGGCCGCCTCGACCGCGATGCCATCGACGAGATCGCCGACTGCTTCGCCAACGGC
AAGAGCTTCGGAGACGCCTG

Downstream 100 bases:

>100_bases
TTGGAGGAACACCATGTCCCTCGTTCACGAAATCGACTACGGCACGCCGGCTTCCAAGTCCGAGAAGCTTGTTTCCCTCA
CCATCGACGGCATGAGCGTC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKSPRIFVPNDATAVACGADRVARKLEAGLLARGITADITRNGSRGLFWLEPLIEVETERGRIGYGPVKPSDIEDLLHAG
ILHGADHPLCVGLVDEIPYLKKQTRLTFARIGIIDPLSLTDYKAHGGYRGLERAITLGPSATVEEVVESGLRGRGGAGFP
TGIKWRTVAAAPADQRYIVCNADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGYVYSRSEYPLAFVVMEKAIEK
AREAGLLGDNILGSGLSFDMEMRMGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHKGLFQKPTVINNLVSLATVPVIL
DKGGKYYRDFGMGRSRGTMPIQIAGNIKFGGLFETAFGIPLGELVYDIGGGTASGRPVKAVQAGGPLGAYLPTHQFDTPF
DYEAFAAKDALIGHGGLVVFDDTADLADMAKFAMEFCAHESCGKCTPCRIGSTRGVETIDKVIAGEKREQNLALLTDLCH
TMKLGSLCALGGFTPYPVLSALNHFPEDFGVAPKLPAAAE

Sequences:

>Translated_520_residues
MKSPRIFVPNDATAVACGADRVARKLEAGLLARGITADITRNGSRGLFWLEPLIEVETERGRIGYGPVKPSDIEDLLHAG
ILHGADHPLCVGLVDEIPYLKKQTRLTFARIGIIDPLSLTDYKAHGGYRGLERAITLGPSATVEEVVESGLRGRGGAGFP
TGIKWRTVAAAPADQRYIVCNADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGYVYSRSEYPLAFVVMEKAIEK
AREAGLLGDNILGSGLSFDMEMRMGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHKGLFQKPTVINNLVSLATVPVIL
DKGGKYYRDFGMGRSRGTMPIQIAGNIKFGGLFETAFGIPLGELVYDIGGGTASGRPVKAVQAGGPLGAYLPTHQFDTPF
DYEAFAAKDALIGHGGLVVFDDTADLADMAKFAMEFCAHESCGKCTPCRIGSTRGVETIDKVIAGEKREQNLALLTDLCH
TMKLGSLCALGGFTPYPVLSALNHFPEDFGVAPKLPAAAE
>Mature_520_residues
MKSPRIFVPNDATAVACGADRVARKLEAGLLARGITADITRNGSRGLFWLEPLIEVETERGRIGYGPVKPSDIEDLLHAG
ILHGADHPLCVGLVDEIPYLKKQTRLTFARIGIIDPLSLTDYKAHGGYRGLERAITLGPSATVEEVVESGLRGRGGAGFP
TGIKWRTVAAAPADQRYIVCNADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGYVYSRSEYPLAFVVMEKAIEK
AREAGLLGDNILGSGLSFDMEMRMGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHKGLFQKPTVINNLVSLATVPVIL
DKGGKYYRDFGMGRSRGTMPIQIAGNIKFGGLFETAFGIPLGELVYDIGGGTASGRPVKAVQAGGPLGAYLPTHQFDTPF
DYEAFAAKDALIGHGGLVVFDDTADLADMAKFAMEFCAHESCGKCTPCRIGSTRGVETIDKVIAGEKREQNLALLTDLCH
TMKLGSLCALGGFTPYPVLSALNHFPEDFGVAPKLPAAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=393, Percent_Identity=38.9312977099237, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI260656005, Length=393, Percent_Identity=38.9312977099237, Blast_Score=263, Evalue=4e-70,
Organism=Escherichia coli, GI1788620, Length=408, Percent_Identity=39.2156862745098, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17531727, Length=421, Percent_Identity=37.0546318289786, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI19920794, Length=387, Percent_Identity=38.7596899224806, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI45550452, Length=384, Percent_Identity=35.9375, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24653819, Length=380, Percent_Identity=28.9473684210526, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24653817, Length=377, Percent_Identity=26.790450928382, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55266; Mature: 55266

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPRIFVPNDATAVACGADRVARKLEAGLLARGITADITRNGSRGLFWLEPLIEVETER
CCCCCEECCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECHHHHEECCC
GRIGYGPVKPSDIEDLLHAGILHGADHPLCVGLVDEIPYLKKQTRLTFARIGIIDPLSLT
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHEEEEEECCCCCCCCC
DYKAHGGYRGLERAITLGPSATVEEVVESGLRGRGGAGFPTGIKWRTVAAAPADQRYIVC
CCCCCCCCHHHHHHEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
NADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGYVYSRSEYPLAFVVMEKAIEK
ECCCCCCCCHHHHHHHCCCCEEEEECCEEEEEEEECCCCEEEECCCCCEEHHHHHHHHHH
AREAGLLGDNILGSGLSFDMEMRMGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHKGL
HHHCCCCCCHHHCCCCCEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCCHHCCC
FQKPTVINNLVSLATVPVILDKGGKYYRDFGMGRSRGTMPIQIAGNIKFGGLFETAFGIP
CCCCHHHHHHHHHHHHEEEECCCCCCHHHHCCCCCCCCEEEEEECCCEECCHHHHHHCCC
LGELVYDIGGGTASGRPVKAVQAGGPLGAYLPTHQFDTPFDYEAFAAKDALIGHGGLVVF
HHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCEEEE
DDTADLADMAKFAMEFCAHESCGKCTPCRIGSTRGVETIDKVIAGEKREQNLALLTDLCH
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
TMKLGSLCALGGFTPYPVLSALNHFPEDFGVAPKLPAAAE
HHHHCCCHHCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MKSPRIFVPNDATAVACGADRVARKLEAGLLARGITADITRNGSRGLFWLEPLIEVETER
CCCCCEECCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECHHHHEECCC
GRIGYGPVKPSDIEDLLHAGILHGADHPLCVGLVDEIPYLKKQTRLTFARIGIIDPLSLT
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHEEEEEECCCCCCCCC
DYKAHGGYRGLERAITLGPSATVEEVVESGLRGRGGAGFPTGIKWRTVAAAPADQRYIVC
CCCCCCCCHHHHHHEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
NADEGDSGTFADRLIMEADPFCLIEGMTIAGLAVGATKGYVYSRSEYPLAFVVMEKAIEK
ECCCCCCCCHHHHHHHCCCCEEEEECCEEEEEEEECCCCEEEECCCCCEEHHHHHHHHHH
AREAGLLGDNILGSGLSFDMEMRMGAGAYVCGEETALLESLEGKRGLVRAKPPLPAHKGL
HHHCCCCCCHHHCCCCCEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCCHHCCC
FQKPTVINNLVSLATVPVILDKGGKYYRDFGMGRSRGTMPIQIAGNIKFGGLFETAFGIP
CCCCHHHHHHHHHHHHEEEECCCCCCHHHHCCCCCCCCEEEEEECCCEECCHHHHHHCCC
LGELVYDIGGGTASGRPVKAVQAGGPLGAYLPTHQFDTPFDYEAFAAKDALIGHGGLVVF
HHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCEEEE
DDTADLADMAKFAMEFCAHESCGKCTPCRIGSTRGVETIDKVIAGEKREQNLALLTDLCH
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
TMKLGSLCALGGFTPYPVLSALNHFPEDFGVAPKLPAAAE
HHHHCCCHHCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]