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The map label for this gene is gsiD [H]

Identifier: 154247307

GI number: 154247307

Start: 3765897

End: 3766784

Strand: Reverse

Name: gsiD [H]

Synonym: Xaut_3379

Alternate gene names: 154247307

Gene position: 3766784-3765897 (Counterclockwise)

Preceding gene: 154247308

Following gene: 154247306

Centisome position: 70.95

GC content: 68.02

Gene sequence:

>888_bases
ATGAGCGCCACATCCGACGCCGCCCTCCAGGCCGCCCCGGTGCAGAAGGCCCGCGGCTATTGGGCCACCGTGGGCCGCCG
CTTTTCCCGCGACAAGGTGAGCATGGTGTGCGCGGCGGTGCTGCTCGCCATCGTGCTCGCCGCCATCTTCGCCCCCTGGC
TCGGCCTTGCCGATCCCTACCAGGGCTCCATGGTCCGCCGCCTCCGCCACATCGGCACGCCGGGCTATCTGCTCGGCACC
GACGAGCTGGGCCGCGACATGCTCTCCCGTCTCATCTATGGCGGGCGCCTCTCGCTGCTCATCGGCATCCTGCCGGTGGT
GCTCGCCTTCGGCATCGGCACCAGCCTCGGCCTGCTCGCCGGCTATCTGGGCGGGGCGGTGAACACCATCATCATGCGCA
CCATCGACGTGTTCTACGCCTTCCCCTCGGTGCTGCTGGCCATCGCCATCTCCGGGGTGCTGGGGGCGGGCATTGTCAAT
TCCATCGTCTCGCTCACCATCGTGTTCGTGCCGCAGATCACCCGCGTGGCGGAGAGCGTGACCACCTCGGTGCGCAACCT
CGACTTCGTGGATGCGGCGCGGGCCTCCGGGGCCGGGGCCATCACCATCATGCGGGTGCACATGCTGGGCAACGTGCTCG
GTCCGATCTTCGTCTATGCGACGGGGCTGATCTCGGTATCCATGATCCTGGCCTCGGGCCTGTCCTTCCTCGGCCTCGGC
ACCAAGCCGCCGGAGCCGGAATGGGGCCTGATGCTGAACACCCTGCGCACCGCCATCTACGTCAACCCGGGCGTGGCCGC
CCTGCCGGGGGTGATGATCTTCGCCGTCTCCATCTGCTTCAACATGCTCAGCGACGGGCTGCGCAGCGCCATGGACATCC
GCAATTGA

Upstream 100 bases:

>100_bases
GGCACCATCCTGGTGCTGGCGCTGTTCTTCGTCTTCCTCAACCTCGTGGTCGATATCGCCCAGGCGTCCATCGATCCGCG
CATCAAGCGGAGCTGAGGAC

Downstream 100 bases:

>100_bases
CCCTCATCGAGCCCCTCCCCGAGCCGATCCTGGAGCCCCTGGCGGACATCGGCGGGCCGGCCCAGCCGCTGCTCGACGTG
AAGAACCTCACCAAGCACTT

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSATSDAALQAAPVQKARGYWATVGRRFSRDKVSMVCAAVLLAIVLAAIFAPWLGLADPYQGSMVRRLRHIGTPGYLLGT
DELGRDMLSRLIYGGRLSLLIGILPVVLAFGIGTSLGLLAGYLGGAVNTIIMRTIDVFYAFPSVLLAIAISGVLGAGIVN
SIVSLTIVFVPQITRVAESVTTSVRNLDFVDAARASGAGAITIMRVHMLGNVLGPIFVYATGLISVSMILASGLSFLGLG
TKPPEPEWGLMLNTLRTAIYVNPGVAALPGVMIFAVSICFNMLSDGLRSAMDIRN

Sequences:

>Translated_295_residues
MSATSDAALQAAPVQKARGYWATVGRRFSRDKVSMVCAAVLLAIVLAAIFAPWLGLADPYQGSMVRRLRHIGTPGYLLGT
DELGRDMLSRLIYGGRLSLLIGILPVVLAFGIGTSLGLLAGYLGGAVNTIIMRTIDVFYAFPSVLLAIAISGVLGAGIVN
SIVSLTIVFVPQITRVAESVTTSVRNLDFVDAARASGAGAITIMRVHMLGNVLGPIFVYATGLISVSMILASGLSFLGLG
TKPPEPEWGLMLNTLRTAIYVNPGVAALPGVMIFAVSICFNMLSDGLRSAMDIRN
>Mature_294_residues
SATSDAALQAAPVQKARGYWATVGRRFSRDKVSMVCAAVLLAIVLAAIFAPWLGLADPYQGSMVRRLRHIGTPGYLLGTD
ELGRDMLSRLIYGGRLSLLIGILPVVLAFGIGTSLGLLAGYLGGAVNTIIMRTIDVFYAFPSVLLAIAISGVLGAGIVNS
IVSLTIVFVPQITRVAESVTTSVRNLDFVDAARASGAGAITIMRVHMLGNVLGPIFVYATGLISVSMILASGLSFLGLGT
KPPEPEWGLMLNTLRTAIYVNPGVAALPGVMIFAVSICFNMLSDGLRSAMDIRN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=290, Percent_Identity=39.6551724137931, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI1787760, Length=282, Percent_Identity=32.6241134751773, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1789964, Length=301, Percent_Identity=37.2093023255814, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789889, Length=217, Percent_Identity=37.3271889400922, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1787498, Length=282, Percent_Identity=32.6241134751773, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1787549, Length=284, Percent_Identity=30.6338028169014, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1788505, Length=220, Percent_Identity=29.0909090909091, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31000; Mature: 30869

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATSDAALQAAPVQKARGYWATVGRRFSRDKVSMVCAAVLLAIVLAAIFAPWLGLADPY
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QGSMVRRLRHIGTPGYLLGTDELGRDMLSRLIYGGRLSLLIGILPVVLAFGIGTSLGLLA
CHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
GYLGGAVNTIIMRTIDVFYAFPSVLLAIAISGVLGAGIVNSIVSLTIVFVPQITRVAESV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTSVRNLDFVDAARASGAGAITIMRVHMLGNVLGPIFVYATGLISVSMILASGLSFLGLG
HHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TKPPEPEWGLMLNTLRTAIYVNPGVAALPGVMIFAVSICFNMLSDGLRSAMDIRN
CCCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SATSDAALQAAPVQKARGYWATVGRRFSRDKVSMVCAAVLLAIVLAAIFAPWLGLADPY
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QGSMVRRLRHIGTPGYLLGTDELGRDMLSRLIYGGRLSLLIGILPVVLAFGIGTSLGLLA
CHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
GYLGGAVNTIIMRTIDVFYAFPSVLLAIAISGVLGAGIVNSIVSLTIVFVPQITRVAESV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTSVRNLDFVDAARASGAGAITIMRVHMLGNVLGPIFVYATGLISVSMILASGLSFLGLG
HHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
TKPPEPEWGLMLNTLRTAIYVNPGVAALPGVMIFAVSICFNMLSDGLRSAMDIRN
CCCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]