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The map label for this gene is phaZ1 [H]

Identifier: 154247272

GI number: 154247272

Start: 3722462

End: 3723751

Strand: Reverse

Name: phaZ1 [H]

Synonym: Xaut_3344

Alternate gene names: 154247272

Gene position: 3723751-3722462 (Counterclockwise)

Preceding gene: 154247273

Following gene: 154247270

Centisome position: 70.14

GC content: 69.22

Gene sequence:

>1290_bases
ATGGCCAGACGATCCAAGATCGACGTTTTGGAAGCCACCCGCCTCACCCGCCAGGGCCGGCTTGAAGAAGCTATGGCCGT
GCTGCGCGGGGACGTCGATACGCCCCACGACGCAGACACGCCGCCTGAAGCGGATGGCAAGGCATTCGTCGACCTGGTGC
CCCCCTCGGCGGCAACCGGAAGCGCCTGGACGACACCGTCCGCCGCCGCGCCGGCGGCCACGGCGCATCGCAGCGAGACC
GGCTCGACTGCCGTCCCGTGGCTGGACGGGCTGCTCGACATGGTCCTGAACCGAACCGCGACGAGCGGGCATGAAGCCCC
GACCCACACCCCGGCCCCAACCGCGCGCGGTGGCGTGGCGGAACCCGCGCCCCAGAAAGCGCGTCTCCATATGCCGTCCG
GCAAAAGCTTCGAGGAGCGCACCTTTGCCAACGAGGCCGGGCGCCGAACCTACAAGCTTTATGTGCCCGGCCGCTTCGAC
GGCCAGCCGCTGCCGCTCCTGGTGATGCTGCACGGCTGCACCCAGTCACCCGATGATTTCGCCACCGGCACCCGGATGAA
CGAGCTCGCGGAAGAGCACGGCTTTTTCGTCGCCTACCCGGCCCAGAGCCACGACGCCAATGCCTCCGGATGCTGGAACT
GGTTCAATGCGGGCGATCAGCGGCGCGACCGGGGCGAACCGTCCCTGATCGCCGGCATCACCCGCCAGATCATGGACGAT
TTCCCGGTCGATCCCGATCGGGTGTTCGCCGCCGGCCTGTCCGCCGGAGGCGCCACGGCGGCGATCATGGGGGCAACCTA
TCCCGATCTCTATCGCGCCATCGGCGTGCATTCCGGACTGGCCTGCGGCGCCGCCCGCGACATGTCCTCCGCCTTTTCAG
CCATGCGCCAGGGCGGCCATCCAAGCGTCGGGACGGTGGTGCATCGCACCGACGGAAAGATCGTGCCCACCATCGTGTTC
CATGGCGACGGCGACAGAACGGTCCATCCCCTGAACGGCGCGCAGGTGGTGGCGCAGGTGGCCGCGCAACTGGCGGCACA
GGCGAAGGCCGAGGAGAAACTGGCGGTGACCATCACCTCCGGCCAGACGCCGGAAGGCATCGGCTACACGCGGGAGGTGC
AGAGCGACGAAACCGGGCGCAGCGTGCTGGAGCACTGGGTGCTCCACGGCGCCGGCCATGCCTGGTCCGGCGGCAGCCCG
TCGGGATCCTACACCGAGCCGCGGGGACCGGACGCCAGCCGCGAAATGGTGCGCTTCTTCCTGCAGACCACATCCGTCCC
GCGCGGCTGA

Upstream 100 bases:

>100_bases
CGTATCCTCTGACTTTTGAAACCACCCGTGCTGGACGCCACCGGCCTCCCCCCGCGCGTCCCCGCGCTTCGCCGAATTGA
ACCGACAGGAGTCTCGCCAC

Downstream 100 bases:

>100_bases
GCGGCCAGCGGGGCCGCTCGAAAACGCCTCAGCCGCCGATGTCGGGCAGCCAGCTTATGGACATGGCGAACGACTGCGCC
GCGCCCGGCGCAATGTGCGC

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MARRSKIDVLEATRLTRQGRLEEAMAVLRGDVDTPHDADTPPEADGKAFVDLVPPSAATGSAWTTPSAAAPAATAHRSET
GSTAVPWLDGLLDMVLNRTATSGHEAPTHTPAPTARGGVAEPAPQKARLHMPSGKSFEERTFANEAGRRTYKLYVPGRFD
GQPLPLLVMLHGCTQSPDDFATGTRMNELAEEHGFFVAYPAQSHDANASGCWNWFNAGDQRRDRGEPSLIAGITRQIMDD
FPVDPDRVFAAGLSAGGATAAIMGATYPDLYRAIGVHSGLACGAARDMSSAFSAMRQGGHPSVGTVVHRTDGKIVPTIVF
HGDGDRTVHPLNGAQVVAQVAAQLAAQAKAEEKLAVTITSGQTPEGIGYTREVQSDETGRSVLEHWVLHGAGHAWSGGSP
SGSYTEPRGPDASREMVRFFLQTTSVPRG

Sequences:

>Translated_429_residues
MARRSKIDVLEATRLTRQGRLEEAMAVLRGDVDTPHDADTPPEADGKAFVDLVPPSAATGSAWTTPSAAAPAATAHRSET
GSTAVPWLDGLLDMVLNRTATSGHEAPTHTPAPTARGGVAEPAPQKARLHMPSGKSFEERTFANEAGRRTYKLYVPGRFD
GQPLPLLVMLHGCTQSPDDFATGTRMNELAEEHGFFVAYPAQSHDANASGCWNWFNAGDQRRDRGEPSLIAGITRQIMDD
FPVDPDRVFAAGLSAGGATAAIMGATYPDLYRAIGVHSGLACGAARDMSSAFSAMRQGGHPSVGTVVHRTDGKIVPTIVF
HGDGDRTVHPLNGAQVVAQVAAQLAAQAKAEEKLAVTITSGQTPEGIGYTREVQSDETGRSVLEHWVLHGAGHAWSGGSP
SGSYTEPRGPDASREMVRFFLQTTSVPRG
>Mature_428_residues
ARRSKIDVLEATRLTRQGRLEEAMAVLRGDVDTPHDADTPPEADGKAFVDLVPPSAATGSAWTTPSAAAPAATAHRSETG
STAVPWLDGLLDMVLNRTATSGHEAPTHTPAPTARGGVAEPAPQKARLHMPSGKSFEERTFANEAGRRTYKLYVPGRFDG
QPLPLLVMLHGCTQSPDDFATGTRMNELAEEHGFFVAYPAQSHDANASGCWNWFNAGDQRRDRGEPSLIAGITRQIMDDF
PVDPDRVFAAGLSAGGATAAIMGATYPDLYRAIGVHSGLACGAARDMSSAFSAMRQGGHPSVGTVVHRTDGKIVPTIVFH
GDGDRTVHPLNGAQVVAQVAAQLAAQAKAEEKLAVTITSGQTPEGIGYTREVQSDETGRSVLEHWVLHGAGHAWSGGSPS
GSYTEPRGPDASREMVRFFLQTTSVPRG

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45403; Mature: 45272

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRSKIDVLEATRLTRQGRLEEAMAVLRGDVDTPHDADTPPEADGKAFVDLVPPSAATG
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
SAWTTPSAAAPAATAHRSETGSTAVPWLDGLLDMVLNRTATSGHEAPTHTPAPTARGGVA
CCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
EPAPQKARLHMPSGKSFEERTFANEAGRRTYKLYVPGRFDGQPLPLLVMLHGCTQSPDDF
CCCCCCCEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHH
ATGTRMNELAEEHGFFVAYPAQSHDANASGCWNWFNAGDQRRDRGEPSLIAGITRQIMDD
HCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHCCCCCHHCCCCCHHHHHHHHHHHHH
FPVDPDRVFAAGLSAGGATAAIMGATYPDLYRAIGVHSGLACGAARDMSSAFSAMRQGGH
CCCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
PSVGTVVHRTDGKIVPTIVFHGDGDRTVHPLNGAQVVAQVAAQLAAQAKAEEKLAVTITS
CCCCEEEECCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
GQTPEGIGYTREVQSDETGRSVLEHWVLHGAGHAWSGGSPSGSYTEPRGPDASREMVRFF
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LQTTSVPRG
HHHCCCCCC
>Mature Secondary Structure 
ARRSKIDVLEATRLTRQGRLEEAMAVLRGDVDTPHDADTPPEADGKAFVDLVPPSAATG
CCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
SAWTTPSAAAPAATAHRSETGSTAVPWLDGLLDMVLNRTATSGHEAPTHTPAPTARGGVA
CCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
EPAPQKARLHMPSGKSFEERTFANEAGRRTYKLYVPGRFDGQPLPLLVMLHGCTQSPDDF
CCCCCCCEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHH
ATGTRMNELAEEHGFFVAYPAQSHDANASGCWNWFNAGDQRRDRGEPSLIAGITRQIMDD
HCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHCCCCCHHCCCCCHHHHHHHHHHHHH
FPVDPDRVFAAGLSAGGATAAIMGATYPDLYRAIGVHSGLACGAARDMSSAFSAMRQGGH
CCCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
PSVGTVVHRTDGKIVPTIVFHGDGDRTVHPLNGAQVVAQVAAQLAAQAKAEEKLAVTITS
CCCCEEEECCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
GQTPEGIGYTREVQSDETGRSVLEHWVLHGAGHAWSGGSPSGSYTEPRGPDASREMVRFF
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LQTTSVPRG
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]