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The map label for this gene is maiA [H]

Identifier: 154247259

GI number: 154247259

Start: 3705497

End: 3706135

Strand: Reverse

Name: maiA [H]

Synonym: Xaut_3331

Alternate gene names: 154247259

Gene position: 3706135-3705497 (Counterclockwise)

Preceding gene: 154247260

Following gene: 154247258

Centisome position: 69.81

GC content: 69.33

Gene sequence:

>639_bases
GTGCGGCTCTATACCTATTGGCGCTCCACCTCGGCCTACCGGGTGCGCATCGCGCTCGCCCTGAAGGGCATCGCGGTGGA
ACAGGTGCCGGTGCATCTGGTGCGCAATGGTGGGGAGCAGCATGCCCCGGCCTATGCCGCCCTCAACCCGCAGCAGCGCC
TGCCGACCCTGGTGCTGGACGACGGCACCGTGCTCACCCAATCCCCGGCCATCCTCGAATATCTGGAGGAGGCCTATCCG
GCCCCGCCCCTGCTGCCGGCCTCGGCAGTGGCGCGGGCGAAGGTGCGCGCGGTGGCGGCGCTCATCGGTTGCGACATCCA
CCCCCTCAACAATGTGTCGCCCCTCACCATGCTGCGCCGCGAGCTGAAGCAGGACGAGGCCGCCGTCTCCGCCTGGATCG
CCCGCTGGATCACGGAGGGCTTTGCCGCCGTGGAACAGATGATCGGCGAGGAGGGCTATTGCTTCGGCCACGCGCCCGGC
CTTGCCGACCTCTACCTGATCCCTCAGCTCTATTCCGCCCGCCGCTTCAACGTCCCGCTGGAGGCTTACCCCCGCATCTT
GCGGGTGGAGGCGCTGGCGGCGACCCATCCTGCCTTCATGGCCGCCCATCCCGCCCGGCAGGCGGATGCGGAAGGGTAG

Upstream 100 bases:

>100_bases
ACACGGTGATCATCACCGGCCGCGCCGCGCGCGAGGGCTTCGTCTCCATCGGCTTCGGCGCCTGCAGTGGCACCATCCTG
CCGGCGCTCGGGTGAGCGCC

Downstream 100 bases:

>100_bases
GTGCCCGGCTTATCCCCGCCGGCGAGTTGCCATCGCGGCAACGCCTCTCTAGTGTCCCGCGCGCCTGCGGCGCTGCCGCC
CGGCCGGCCCACGAACGGGA

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRLYTYWRSTSAYRVRIALALKGIAVEQVPVHLVRNGGEQHAPAYAALNPQQRLPTLVLDDGTVLTQSPAILEYLEEAYP
APPLLPASAVARAKVRAVAALIGCDIHPLNNVSPLTMLRRELKQDEAAVSAWIARWITEGFAAVEQMIGEEGYCFGHAPG
LADLYLIPQLYSARRFNVPLEAYPRILRVEALAATHPAFMAAHPARQADAEG

Sequences:

>Translated_212_residues
MRLYTYWRSTSAYRVRIALALKGIAVEQVPVHLVRNGGEQHAPAYAALNPQQRLPTLVLDDGTVLTQSPAILEYLEEAYP
APPLLPASAVARAKVRAVAALIGCDIHPLNNVSPLTMLRRELKQDEAAVSAWIARWITEGFAAVEQMIGEEGYCFGHAPG
LADLYLIPQLYSARRFNVPLEAYPRILRVEALAATHPAFMAAHPARQADAEG
>Mature_212_residues
MRLYTYWRSTSAYRVRIALALKGIAVEQVPVHLVRNGGEQHAPAYAALNPQQRLPTLVLDDGTVLTQSPAILEYLEEAYP
APPLLPASAVARAKVRAVAALIGCDIHPLNNVSPLTMLRRELKQDEAAVSAWIARWITEGFAAVEQMIGEEGYCFGHAPG
LADLYLIPQLYSARRFNVPLEAYPRILRVEALAATHPAFMAAHPARQADAEG

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=210, Percent_Identity=43.3333333333333, Blast_Score=169, Evalue=1e-42,
Organism=Homo sapiens, GI194440732, Length=210, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI22202622, Length=161, Percent_Identity=39.7515527950311, Blast_Score=108, Evalue=3e-24,
Organism=Escherichia coli, GI1789624, Length=171, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=40.2843601895735, Blast_Score=150, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17510461, Length=210, Percent_Identity=38.0952380952381, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17556142, Length=211, Percent_Identity=36.9668246445498, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355859, Length=210, Percent_Identity=43.3333333333333, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24645375, Length=210, Percent_Identity=43.3333333333333, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI45553325, Length=210, Percent_Identity=43.3333333333333, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI21355857, Length=211, Percent_Identity=37.4407582938389, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23231; Mature: 23231

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYTYWRSTSAYRVRIALALKGIAVEQVPVHLVRNGGEQHAPAYAALNPQQRLPTLVLD
CEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHCCEEEEC
DGTVLTQSPAILEYLEEAYPAPPLLPASAVARAKVRAVAALIGCDIHPLNNVSPLTMLRR
CCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ELKQDEAAVSAWIARWITEGFAAVEQMIGEEGYCFGHAPGLADLYLIPQLYSARRFNVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCH
EAYPRILRVEALAATHPAFMAAHPARQADAEG
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRLYTYWRSTSAYRVRIALALKGIAVEQVPVHLVRNGGEQHAPAYAALNPQQRLPTLVLD
CEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHCCEEEEC
DGTVLTQSPAILEYLEEAYPAPPLLPASAVARAKVRAVAALIGCDIHPLNNVSPLTMLRR
CCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ELKQDEAAVSAWIARWITEGFAAVEQMIGEEGYCFGHAPGLADLYLIPQLYSARRFNVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCH
EAYPRILRVEALAATHPAFMAAHPARQADAEG
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]