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The map label for this gene is ydgK [H]

Identifier: 154247154

GI number: 154247154

Start: 3574641

End: 3575927

Strand: Reverse

Name: ydgK [H]

Synonym: Xaut_3226

Alternate gene names: 154247154

Gene position: 3575927-3574641 (Counterclockwise)

Preceding gene: 154247156

Following gene: 154247153

Centisome position: 67.36

GC content: 67.99

Gene sequence:

>1287_bases
ATGCCAGAGCGTTCCATGTCCCATCCCGAGCGCGCCCCCGCGCCCCGCAACGCGTCCATCCCGGCGAAGGCGACGGCGCC
CACCCGGCCGCCGTTCCTCGAATTCGTCGCCCTCATCGCCCTGCTCATCGGCATGACGGCCTTCTCCATCGACAACCTCT
TGCCCGCCTTCGGCGCCATCCGGGAGGACTTCGCCCTCGCCAACGCCAATGACGTGCAGGTGATGGTGTATGCCTACCTG
CTAGGCATCGGCTCCGCGCAGTTCCTCTACGGCCCCATCTCCGACGTGGTGGGCCGCCGGCCGATCCTGTTCGCGGGCCT
CGCCATCTATGCGGTGGGCACGCTGATTGCCCTGTTCACACGGGATTTCAACGTGCTGATCGCCGCGCGCGTGATCCAGG
GCGTGGGCGCGGCCTCGGGCCGGGTGCTGGCCATCGCCATCGTGCGCGACCGGTTCGAGGGGCGCGAGATGGCGCGCATC
ATGTCACTGGCCATGATGGTGTTCCTCACCGTGCCCATCCTGGCGCCGGCGCTGGGCAGCCTGATCCTCGCCTTCGGCTC
CTGGCACCTCATCTTCATCGCCATGCTCACGCTGGCGGTGGCGCTGCTGGTCTGGTTCGGCCTGCGCATGCCGGAGACGC
TGCATCCAGAATATCGCATCCCCTTCTCGTTCCGGGCCATCGGCAAGGCCGTGGTGCTCACCCTCACCACCCGCCGCGCG
GCAGGCTATGCGGTGGCCATCGGCCTGATGATGGGATCGCTGATGGCCTATGTGGGCTCCGCCTCCCAGGTGTTCCAGAC
CGATGTCTACAAGCTGGGCAACCTGTTCCCGGTGGCGTTCGCCGCGGTGGCGGGCATCATGGCGGTGGCCTCCTTCACCA
ACGCCGCGCTGGTGCGGCGGATCGGCATGCGGCGGCTGTCCCATGGCGGCATCTGCGGCTTCATCACCGTGGGGGCCCTG
ATGGTGGCAGCCAGCCTCGCCTATGGCGGCAAGCCGCCGCTGATCCTGTTCTGCGGCCTCGTCGGGGCGGGGCAGTTCCT
GTTCGCGCTCACGGTGCCGAACTTCAATTCCATGGCCATGGAGCCGCTGGGCGCGGTGGCCGGAACCGCCTCCTCCTTCA
TCGGCGGCTTCACCACCCTGATGGCGTCCGTGCTCGGCTATTTCGTGGGCCGCGCCTTCGACGGCACGGTGCTGCCGCTG
AGCCTGGGCTACATGGTGCTCGGCGCCATCGCGCTGGTGTTCGTGCTGTGGGCCGAGAAGGGTCGGCTGTTCGGGCACAA
TCAATAG

Upstream 100 bases:

>100_bases
GGCGCCATCGCCTATGTGTCCTTGGCCCATCCGCCCCCTTGCCGGTCGCCCCTCCCCGCGCGCCGCGCCTCACAGCCGTG
TCTGTTGCGGGACACGTCCA

Downstream 100 bases:

>100_bases
GCGGCTAATCATAGCGCCGGAGCGGTTCCCCGCTCCGTGCCTGCTGCATGGGTGCCCCGCCCGCCGTGGCGGATTCTGTC
GGCGAACTGTCCGCAAGCCG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPERSMSHPERAPAPRNASIPAKATAPTRPPFLEFVALIALLIGMTAFSIDNLLPAFGAIREDFALANANDVQVMVYAYL
LGIGSAQFLYGPISDVVGRRPILFAGLAIYAVGTLIALFTRDFNVLIAARVIQGVGAASGRVLAIAIVRDRFEGREMARI
MSLAMMVFLTVPILAPALGSLILAFGSWHLIFIAMLTLAVALLVWFGLRMPETLHPEYRIPFSFRAIGKAVVLTLTTRRA
AGYAVAIGLMMGSLMAYVGSASQVFQTDVYKLGNLFPVAFAAVAGIMAVASFTNAALVRRIGMRRLSHGGICGFITVGAL
MVAASLAYGGKPPLILFCGLVGAGQFLFALTVPNFNSMAMEPLGAVAGTASSFIGGFTTLMASVLGYFVGRAFDGTVLPL
SLGYMVLGAIALVFVLWAEKGRLFGHNQ

Sequences:

>Translated_428_residues
MPERSMSHPERAPAPRNASIPAKATAPTRPPFLEFVALIALLIGMTAFSIDNLLPAFGAIREDFALANANDVQVMVYAYL
LGIGSAQFLYGPISDVVGRRPILFAGLAIYAVGTLIALFTRDFNVLIAARVIQGVGAASGRVLAIAIVRDRFEGREMARI
MSLAMMVFLTVPILAPALGSLILAFGSWHLIFIAMLTLAVALLVWFGLRMPETLHPEYRIPFSFRAIGKAVVLTLTTRRA
AGYAVAIGLMMGSLMAYVGSASQVFQTDVYKLGNLFPVAFAAVAGIMAVASFTNAALVRRIGMRRLSHGGICGFITVGAL
MVAASLAYGGKPPLILFCGLVGAGQFLFALTVPNFNSMAMEPLGAVAGTASSFIGGFTTLMASVLGYFVGRAFDGTVLPL
SLGYMVLGAIALVFVLWAEKGRLFGHNQ
>Mature_427_residues
PERSMSHPERAPAPRNASIPAKATAPTRPPFLEFVALIALLIGMTAFSIDNLLPAFGAIREDFALANANDVQVMVYAYLL
GIGSAQFLYGPISDVVGRRPILFAGLAIYAVGTLIALFTRDFNVLIAARVIQGVGAASGRVLAIAIVRDRFEGREMARIM
SLAMMVFLTVPILAPALGSLILAFGSWHLIFIAMLTLAVALLVWFGLRMPETLHPEYRIPFSFRAIGKAVVLTLTTRRAA
GYAVAIGLMMGSLMAYVGSASQVFQTDVYKLGNLFPVAFAAVAGIMAVASFTNAALVRRIGMRRLSHGGICGFITVGALM
VAASLAYGGKPPLILFCGLVGAGQFLFALTVPNFNSMAMEPLGAVAGTASSFIGGFTTLMASVLGYFVGRAFDGTVLPLS
LGYMVLGAIALVFVLWAEKGRLFGHNQ

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=390, Percent_Identity=26.4102564102564, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI48994889, Length=154, Percent_Identity=36.3636363636364, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082312, Length=177, Percent_Identity=35.0282485875706, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790146, Length=166, Percent_Identity=30.7228915662651, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787065, Length=260, Percent_Identity=28.8461538461538, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790794, Length=179, Percent_Identity=27.9329608938547, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45353; Mature: 45222

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERSMSHPERAPAPRNASIPAKATAPTRPPFLEFVALIALLIGMTAFSIDNLLPAFGAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REDFALANANDVQVMVYAYLLGIGSAQFLYGPISDVVGRRPILFAGLAIYAVGTLIALFT
HHHHHCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RDFNVLIAARVIQGVGAASGRVLAIAIVRDRFEGREMARIMSLAMMVFLTVPILAPALGS
CCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILAFGSWHLIFIAMLTLAVALLVWFGLRMPETLHPEYRIPFSFRAIGKAVVLTLTTRRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHCCCCEEEEECCHH
AGYAVAIGLMMGSLMAYVGSASQVFQTDVYKLGNLFPVAFAAVAGIMAVASFTNAALVRR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGMRRLSHGGICGFITVGALMVAASLAYGGKPPLILFCGLVGAGQFLFALTVPNFNSMAM
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECHHCCCCEEEEEECCCCCCHHH
EPLGAVAGTASSFIGGFTTLMASVLGYFVGRAFDGTVLPLSLGYMVLGAIALVFVLWAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GRLFGHNQ
CCCCCCCC
>Mature Secondary Structure 
PERSMSHPERAPAPRNASIPAKATAPTRPPFLEFVALIALLIGMTAFSIDNLLPAFGAI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REDFALANANDVQVMVYAYLLGIGSAQFLYGPISDVVGRRPILFAGLAIYAVGTLIALFT
HHHHHCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RDFNVLIAARVIQGVGAASGRVLAIAIVRDRFEGREMARIMSLAMMVFLTVPILAPALGS
CCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILAFGSWHLIFIAMLTLAVALLVWFGLRMPETLHPEYRIPFSFRAIGKAVVLTLTTRRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHCCCCEEEEECCHH
AGYAVAIGLMMGSLMAYVGSASQVFQTDVYKLGNLFPVAFAAVAGIMAVASFTNAALVRR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGMRRLSHGGICGFITVGALMVAASLAYGGKPPLILFCGLVGAGQFLFALTVPNFNSMAM
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECHHCCCCEEEEEECCCCCCHHH
EPLGAVAGTASSFIGGFTTLMASVLGYFVGRAFDGTVLPLSLGYMVLGAIALVFVLWAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GRLFGHNQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]