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The map label for this gene is cueO [H]

Identifier: 154247125

GI number: 154247125

Start: 3543518

End: 3544990

Strand: Reverse

Name: cueO [H]

Synonym: Xaut_3196

Alternate gene names: 154247125

Gene position: 3544990-3543518 (Counterclockwise)

Preceding gene: 154247126

Following gene: 154247124

Centisome position: 66.77

GC content: 68.02

Gene sequence:

>1473_bases
GTGAAAATCTCGCGACGGGCGGTCCTGCTCGGTGCCACGGCGGCGGGGGCGGCAAGCTTTGCCCCCTCCGCCCTCGGGCG
CATGCCCCTCTCGCGCGACACGCTCCCCAAGAGCGCGCCGGTGCGGCGCCTCACCCTCTCCGCAGCGGAGATGGAGGTGC
CGCTCCTCGGCCCCGACAAGCCGAAGACGTCAGGCTTCTGCGCCTACAACGGCACACCCTTTCTGACCTGGCGCGTGCCG
GTGGGCACAAGGCTGCTGGTGGATTTCGAGAACCGCCTGCCAAACCCCACCACGGTGCACTGGCACGGCATCCGTGTGCC
CTATCTCTCCGACGGCGTCGCGCCCCTCACCCAGCAGCCGATCCCGGCCGGCGGGCGCTTCAGCTACGACGTGCCCCTGC
CCGATCCGGGCTTCTACTTCTTCCATCCCCATTGCGACGAAACCGGCCAGGTGGGACGTGGCCTCTTCGCCCTGCTGCTG
GTGGACGATCCGGCCGAGGCCAGGCTCGGCTTCGACCTTGAGCATATCATCGCGGTCAAGGACTGGCGGCTGGATGCGGA
CGGCCGCTGGCTGCCCATCTTCACCGACGAAGGCGCCTCCACGGCCGGTACCTTCGGCACGGTGCGCGCCACCAACGGGT
CGGTGATCCCGCCGCGGGTCGAGGCCCCGGCCTATGGCGACGTGCGGCTGCGGGTGATCGTCGCCGATTCCACCCGCACC
ATTGAATTCGGCTGCGAGACCGGCGACGCCGCCATCATTGCCGTGGACGGGCAGGCTCTCACCCCGCTCCCCCTGTCCAA
GCTGCCGGATGAGGTCTGGCGCATGGGCCCGGCCATGCGGCTCGACGTGCATGTGCGCATGCCCGGCCCCGGCGAGGAGG
TGAAGATCTTCGACTATCGCACCGCCGAGCCCTATCTGCTCACCACCCTGGTGGCGGTGGACAAGGGAAAGAAGCAGACG
CCCTTCAAGCCCATCGCGCTGCCCGCCGCGAAGGCGCCGCTGCCCGACGTTCGCCGCGCGAAGAAACTCGATTTCATCGT
GCAGACCTCCGCCGGCGCCACCGCCATCGCGCCGCCCCTGCCCCCGGAGGATCCACTGGCGAAGGCGCTGATCGATTCCT
TCTGCGTGGGCGACAAGACCTTCTGGGCCATCAACCGCACGTCGTGGGGCGCGGGCGCCGACCTGCGTCTGCCGCCGCCG
CTCGATATCTTGAAGGATGGCGACAGCTACCGGCTCACCATCACCAACGTCACCAAGCATGTGCATCCCATGCACCTGCA
CGGCCATGTCTTCCGCGTGCTGTCCTCCACCCGCAAGACCAGCATCCCGCCCTATCTCGCCGACACCGTGATGACCGAGC
CCAACGAGACGGTGGAGATCGCCTTCAAGGCCAACAGCGGCGACTGGGTGTTCCATTGCCACATTCTGGAGCACATGGAT
GCCGGACTGATGGGCTGGTTCCGGGTGGCATGA

Upstream 100 bases:

>100_bases
GCCATCGAGCGCGGGGTCTCGGCCCTCAAGCTGCAGATCAAGGTGGAAGGCTCGGCCAGTCTCGACAACCCGAAGGCCGT
GAAGAAGAAATGAGGCTGAG

Downstream 100 bases:

>100_bases
TGGGACGGATGGGACTTCGTGCGGCCCTCGCGCTTGCCCTGCTGGCGACACCAGTCGCGGCGCAGAACCCGCCGGCGCAG
CCGCTGGACAAGCTCGACAT

Product: multicopper oxidase type 2

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MKISRRAVLLGATAAGAASFAPSALGRMPLSRDTLPKSAPVRRLTLSAAEMEVPLLGPDKPKTSGFCAYNGTPFLTWRVP
VGTRLLVDFENRLPNPTTVHWHGIRVPYLSDGVAPLTQQPIPAGGRFSYDVPLPDPGFYFFHPHCDETGQVGRGLFALLL
VDDPAEARLGFDLEHIIAVKDWRLDADGRWLPIFTDEGASTAGTFGTVRATNGSVIPPRVEAPAYGDVRLRVIVADSTRT
IEFGCETGDAAIIAVDGQALTPLPLSKLPDEVWRMGPAMRLDVHVRMPGPGEEVKIFDYRTAEPYLLTTLVAVDKGKKQT
PFKPIALPAAKAPLPDVRRAKKLDFIVQTSAGATAIAPPLPPEDPLAKALIDSFCVGDKTFWAINRTSWGAGADLRLPPP
LDILKDGDSYRLTITNVTKHVHPMHLHGHVFRVLSSTRKTSIPPYLADTVMTEPNETVEIAFKANSGDWVFHCHILEHMD
AGLMGWFRVA

Sequences:

>Translated_490_residues
MKISRRAVLLGATAAGAASFAPSALGRMPLSRDTLPKSAPVRRLTLSAAEMEVPLLGPDKPKTSGFCAYNGTPFLTWRVP
VGTRLLVDFENRLPNPTTVHWHGIRVPYLSDGVAPLTQQPIPAGGRFSYDVPLPDPGFYFFHPHCDETGQVGRGLFALLL
VDDPAEARLGFDLEHIIAVKDWRLDADGRWLPIFTDEGASTAGTFGTVRATNGSVIPPRVEAPAYGDVRLRVIVADSTRT
IEFGCETGDAAIIAVDGQALTPLPLSKLPDEVWRMGPAMRLDVHVRMPGPGEEVKIFDYRTAEPYLLTTLVAVDKGKKQT
PFKPIALPAAKAPLPDVRRAKKLDFIVQTSAGATAIAPPLPPEDPLAKALIDSFCVGDKTFWAINRTSWGAGADLRLPPP
LDILKDGDSYRLTITNVTKHVHPMHLHGHVFRVLSSTRKTSIPPYLADTVMTEPNETVEIAFKANSGDWVFHCHILEHMD
AGLMGWFRVA
>Mature_490_residues
MKISRRAVLLGATAAGAASFAPSALGRMPLSRDTLPKSAPVRRLTLSAAEMEVPLLGPDKPKTSGFCAYNGTPFLTWRVP
VGTRLLVDFENRLPNPTTVHWHGIRVPYLSDGVAPLTQQPIPAGGRFSYDVPLPDPGFYFFHPHCDETGQVGRGLFALLL
VDDPAEARLGFDLEHIIAVKDWRLDADGRWLPIFTDEGASTAGTFGTVRATNGSVIPPRVEAPAYGDVRLRVIVADSTRT
IEFGCETGDAAIIAVDGQALTPLPLSKLPDEVWRMGPAMRLDVHVRMPGPGEEVKIFDYRTAEPYLLTTLVAVDKGKKQT
PFKPIALPAAKAPLPDVRRAKKLDFIVQTSAGATAIAPPLPPEDPLAKALIDSFCVGDKTFWAINRTSWGAGADLRLPPP
LDILKDGDSYRLTITNVTKHVHPMHLHGHVFRVLSSTRKTSIPPYLADTVMTEPNETVEIAFKANSGDWVFHCHILEHMD
AGLMGWFRVA

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=550, Percent_Identity=27.8181818181818, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574104, Length=119, Percent_Identity=37.8151260504202, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 53383; Mature: 53383

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00080 MULTICOPPER_OXIDASE2 ; PS00498 TYROSINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISRRAVLLGATAAGAASFAPSALGRMPLSRDTLPKSAPVRRLTLSAAEMEVPLLGPDK
CCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEHHHEEEEECCCCC
PKTSGFCAYNGTPFLTWRVPVGTRLLVDFENRLPNPTTVHWHGIRVPYLSDGVAPLTQQP
CCCCEEEEECCCEEEEEECCCCCEEEEEHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCC
IPAGGRFSYDVPLPDPGFYFFHPHCDETGQVGRGLFALLLVDDPAEARLGFDLEHIIAVK
CCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCHHHCCCCHHHEEEEE
DWRLDADGRWLPIFTDEGASTAGTFGTVRATNGSVIPPRVEAPAYGDVRLRVIVADSTRT
EEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCEE
IEFGCETGDAAIIAVDGQALTPLPLSKLPDEVWRMGPAMRLDVHVRMPGPGEEVKIFDYR
EEEECCCCCEEEEEECCCCCCCCCHHHCCHHHHHCCCCEEEEEEEECCCCCCEEEEEEEC
TAEPYLLTTLVAVDKGKKQTPFKPIALPAAKAPLPDVRRAKKLDFIVQTSAGATAIAPPL
CCCCEEEEEEEEECCCCCCCCCCCEECCCCCCCCCHHHHHHHEEEEEEECCCCEEECCCC
PPEDPLAKALIDSFCVGDKTFWAINRTSWGAGADLRLPPPLDILKDGDSYRLTITNVTKH
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCC
VHPMHLHGHVFRVLSSTRKTSIPPYLADTVMTEPNETVEIAFKANSGDWVFHCHILEHMD
CCEEEHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCEEEEEEEECCCCEEEEEEEHHHCC
AGLMGWFRVA
CCCCEEEEEC
>Mature Secondary Structure
MKISRRAVLLGATAAGAASFAPSALGRMPLSRDTLPKSAPVRRLTLSAAEMEVPLLGPDK
CCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEHHHEEEEECCCCC
PKTSGFCAYNGTPFLTWRVPVGTRLLVDFENRLPNPTTVHWHGIRVPYLSDGVAPLTQQP
CCCCEEEEECCCEEEEEECCCCCEEEEEHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCC
IPAGGRFSYDVPLPDPGFYFFHPHCDETGQVGRGLFALLLVDDPAEARLGFDLEHIIAVK
CCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCHHHCCCCHHHEEEEE
DWRLDADGRWLPIFTDEGASTAGTFGTVRATNGSVIPPRVEAPAYGDVRLRVIVADSTRT
EEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCEE
IEFGCETGDAAIIAVDGQALTPLPLSKLPDEVWRMGPAMRLDVHVRMPGPGEEVKIFDYR
EEEECCCCCEEEEEECCCCCCCCCHHHCCHHHHHCCCCEEEEEEEECCCCCCEEEEEEEC
TAEPYLLTTLVAVDKGKKQTPFKPIALPAAKAPLPDVRRAKKLDFIVQTSAGATAIAPPL
CCCCEEEEEEEEECCCCCCCCCCCEECCCCCCCCCHHHHHHHEEEEEEECCCCEEECCCC
PPEDPLAKALIDSFCVGDKTFWAINRTSWGAGADLRLPPPLDILKDGDSYRLTITNVTKH
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCC
VHPMHLHGHVFRVLSSTRKTSIPPYLADTVMTEPNETVEIAFKANSGDWVFHCHILEHMD
CCEEEHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCEEEEEEEECCCCEEEEEEEHHHCC
AGLMGWFRVA
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]