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The map label for this gene is yafV [C]

Identifier: 154247017

GI number: 154247017

Start: 3429120

End: 3430052

Strand: Reverse

Name: yafV [C]

Synonym: Xaut_3088

Alternate gene names: 154247017

Gene position: 3430052-3429120 (Counterclockwise)

Preceding gene: 154247018

Following gene: 154247016

Centisome position: 64.61

GC content: 69.13

Gene sequence:

>933_bases
ATGACGGATCAGGGCGGGTTCAGCATCCAGGAAAGAGCGGGCCTCATGACCTCGGCCGACACATCCGACACCCTCCGCAT
CGCACTGGTGCAGTTGCGCAGCGGCCGGGATCCGGCAGCCAATCTCGACGCCACCGTAGCCCTGGTGCGCGAGGCCGCGC
GGGACGGGGCGCGCTATGTGCAGACGCCCGAGGTCACCAACCTCATGGAGCTGGACCGCGCCGTCCTGTTCGAGAAGCTG
CGGGAGGAGGCGGACGACGCCACCCTCAAAGCCCTGCGCCAGCTGGCGCAGGAGCTGGGCATCTTCCTCCACATCGGCTC
GCTGGCCCTGAAGGTGTCGGACAAGAAGGCGGTCAACCGCGCCTTCATGGTCGGACCCGACGGCGAGATCATCGCCCGCT
ACGACAAGATCCACATGTTCGACGTGGATCTGGGCAACGGCGAAAGCTATCGCGAATCCTCCGCCTACCGCCCCGGCGAG
CGGGCGATCCTGGCCAATGTGGGCGCGTTCCGCTTCGGCCTCACCATCTGCTACGACCTGCGCTTCCCCTCCCTCTACCG
GGCCCTGGCCGAGGGTGGAGCGCAAGTGCTCACCGTGCCATCCGCCTTCACCCGGCCCACCGGGGAGGCGCACTGGCACG
TGCTGCTGCGGGCGCGGGCCATCGAGAACGGCGCCTATGTGCTGGCGGCCGCCCAGGGCGGCAAGCATGAGAACGGCCGG
GAGACCTTCGGCCATTCGCTGGTGGTGGACCCCTGGGGCCGTGTGATCGCCGAAGGCGGCGCCGACCCCGGCGTGATCCT
CGCCGAGATCGACCTGAAGGAAGTCGCCGCCGCTCGCGCGCGCATCCCCTCCCTCTCCAACGGCCGCCGGTTCGAGATCG
CCGAGCCGGGCCAGACCATCGGCCACCTCAGCGTGGTGCGGGCTTCGTCATGA

Upstream 100 bases:

>100_bases
GCCTGAGCTTTTCTTTCCGTGATGCGCTGACCCGTGCAAGACTAGAAAAGGGCCCCATCTATCTGGGGAGACGCCAGCAT
CGGGCGCGCGCGGCGTGCGC

Downstream 100 bases:

>100_bases
TCCGCTACACCCTCAATTGCGCGTCGGGCCACCAGTTCGAGAGCTGGTTTCCCTCCTCGGCGAGCTTTGATGCCCAGCAG
GCGCGCGGGCTGGTCAGCTG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTDQGGFSIQERAGLMTSADTSDTLRIALVQLRSGRDPAANLDATVALVREAARDGARYVQTPEVTNLMELDRAVLFEKL
REEADDATLKALRQLAQELGIFLHIGSLALKVSDKKAVNRAFMVGPDGEIIARYDKIHMFDVDLGNGESYRESSAYRPGE
RAILANVGAFRFGLTICYDLRFPSLYRALAEGGAQVLTVPSAFTRPTGEAHWHVLLRARAIENGAYVLAAAQGGKHENGR
ETFGHSLVVDPWGRVIAEGGADPGVILAEIDLKEVAAARARIPSLSNGRRFEIAEPGQTIGHLSVVRASS

Sequences:

>Translated_310_residues
MTDQGGFSIQERAGLMTSADTSDTLRIALVQLRSGRDPAANLDATVALVREAARDGARYVQTPEVTNLMELDRAVLFEKL
REEADDATLKALRQLAQELGIFLHIGSLALKVSDKKAVNRAFMVGPDGEIIARYDKIHMFDVDLGNGESYRESSAYRPGE
RAILANVGAFRFGLTICYDLRFPSLYRALAEGGAQVLTVPSAFTRPTGEAHWHVLLRARAIENGAYVLAAAQGGKHENGR
ETFGHSLVVDPWGRVIAEGGADPGVILAEIDLKEVAAARARIPSLSNGRRFEIAEPGQTIGHLSVVRASS
>Mature_309_residues
TDQGGFSIQERAGLMTSADTSDTLRIALVQLRSGRDPAANLDATVALVREAARDGARYVQTPEVTNLMELDRAVLFEKLR
EEADDATLKALRQLAQELGIFLHIGSLALKVSDKKAVNRAFMVGPDGEIIARYDKIHMFDVDLGNGESYRESSAYRPGER
AILANVGAFRFGLTICYDLRFPSLYRALAEGGAQVLTVPSAFTRPTGEAHWHVLLRARAIENGAYVLAAAQGGKHENGRE
TFGHSLVVDPWGRVIAEGGADPGVILAEIDLKEVAAARARIPSLSNGRRFEIAEPGQTIGHLSVVRASS

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=296, Percent_Identity=38.8513513513513, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI297632350, Length=282, Percent_Identity=38.6524822695035, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI5031947, Length=282, Percent_Identity=38.6524822695035, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI9910460, Length=266, Percent_Identity=35.3383458646617, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI297632346, Length=202, Percent_Identity=34.6534653465347, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786412, Length=211, Percent_Identity=29.8578199052133, Blast_Score=81, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556280, Length=288, Percent_Identity=36.4583333333333, Blast_Score=168, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6323383, Length=278, Percent_Identity=33.8129496402878, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322335, Length=304, Percent_Identity=32.2368421052632, Blast_Score=155, Evalue=7e-39,
Organism=Drosophila melanogaster, GI17933642, Length=284, Percent_Identity=38.7323943661972, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21355835, Length=273, Percent_Identity=28.5714285714286, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 33535; Mature: 33404

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQGGFSIQERAGLMTSADTSDTLRIALVQLRSGRDPAANLDATVALVREAARDGARYV
CCCCCCCCHHHHCCCEECCCCCCHHEEEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCEE
QTPEVTNLMELDRAVLFEKLREEADDATLKALRQLAQELGIFLHIGSLALKVSDKKAVNR
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECEEEEEECCHHHHCE
AFMVGPDGEIIARYDKIHMFDVDLGNGESYRESSAYRPGERAILANVGAFRFGLTICYDL
EEEECCCCCEEEEEEEEEEEEEECCCCCCHHHCCCCCCCCCEEEECCCHHHEEEEEEEEC
RFPSLYRALAEGGAQVLTVPSAFTRPTGEAHWHVLLRARAIENGAYVLAAAQGGKHENGR
CCHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCH
ETFGHSLVVDPWGRVIAEGGADPGVILAEIDLKEVAAARARIPSLSNGRRFEIAEPGQTI
HHHCCCEEECCHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEECCCCCCC
GHLSVVRASS
CEEEEEEECC
>Mature Secondary Structure 
TDQGGFSIQERAGLMTSADTSDTLRIALVQLRSGRDPAANLDATVALVREAARDGARYV
CCCCCCCHHHHCCCEECCCCCCHHEEEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCEE
QTPEVTNLMELDRAVLFEKLREEADDATLKALRQLAQELGIFLHIGSLALKVSDKKAVNR
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECEEEEEECCHHHHCE
AFMVGPDGEIIARYDKIHMFDVDLGNGESYRESSAYRPGERAILANVGAFRFGLTICYDL
EEEECCCCCEEEEEEEEEEEEEECCCCCCHHHCCCCCCCCCEEEECCCHHHEEEEEEEEC
RFPSLYRALAEGGAQVLTVPSAFTRPTGEAHWHVLLRARAIENGAYVLAAAQGGKHENGR
CCHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEEEEEEEECCCCEEEEEECCCCCCCCH
ETFGHSLVVDPWGRVIAEGGADPGVILAEIDLKEVAAARARIPSLSNGRRFEIAEPGQTI
HHHCCCEEECCHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEECCCCCCC
GHLSVVRASS
CEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]