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The map label for this gene is pgk [H]

Identifier: 154247005

GI number: 154247005

Start: 3415163

End: 3416353

Strand: Direct

Name: pgk [H]

Synonym: Xaut_3076

Alternate gene names: 154247005

Gene position: 3415163-3416353 (Clockwise)

Preceding gene: 154247004

Following gene: 154247006

Centisome position: 64.33

GC content: 69.19

Gene sequence:

>1191_bases
ATGACCGCCTTCCGAACCCTCGACGACGCGGACCTTGCCGACAAGCGGGTCCTCGTGCGCGTTGACCTCAACGTGCCCAT
GGAAAGCGGGCGGGTCACCGACGAGACCCGCCTGAAAGCCATCCTGCCCACCATCCGCACCATCACGGACAAGGGCGGCA
AGGCGGTGCTACTGGCGCATTTCGGGCGGCCCAAGGGCCGCGACGAGAGCCAGTCCCTCGCCCCGGTGGCGAAGGCGCTG
GAAGGCCAGCTCGGCCGCAAGGTGGCGTTCGCCTCCGATTGCGTGGGCGAGGAAGCCTCCAGCGCCATCTCCCGGCTCGC
CGCCGGCGAGGTGATCGTGCTGGAAAACACCCGCTTCCACGCCGGTGAGGAGAAGAACGCGCCTGATTTCGTGGAGGCCC
TCGCCAGCCTCGGCGACATCTATGTCAACGACGCCTTCTCGGCCGCCCATCGCGCCCATGCCTCCACCGAGGGGCTGGCC
CGCAAGCTGCCGGCCTATGCCGGCCGCTCCATGGAAAGCGAGCTCGCCGCGCTCACCAAGGCCCTTGAGGCCCCGGTGCG
TCCGGTGCTGGCCGTGGTCGGTGGCTCCAAGGTGTCCTCCAAGCTCGAGCTTCTCGGGAACCTCGTGAAGAAGGTGGACA
TCCTGGTGATCGCCGGCGGCATGGCCAACACCTTCCTTGCCGCCCTCGGCAAGAAGGTGGGCAAGTCGCTGTGCGAGCAC
GACCTGGCCGACACCGCCCGCGAGATTCTGGAGAAGGCCAAGGCGGCCGGCTGCGAGATCGTGCTGCCGGTGGACGCGGT
GGTGGCTAAGGAGTTCAAGGCCAATGCCGCGAACCGCGTCGTCAGCGTGGACGAGGTGGGCGACGACGAGATGATCCTCG
ACGCCGGTCCCGAAACCGTGGCGGTGGTGGCGCAGAAGCTCGACGGCGCCAAGACCGTGGTCTGGAACGGCCCGTTCGGC
GCCTTCGAGATGACGCCCTTCGACGCCGCGACGGTGGCGGTTGCCCGCGACGTGGGCAAGCGCACCCGCGCCGGCACGCT
GCTGTCGGTAGCCGGCGGCGGCGACACGGTGGCCGCGCTCAACCATGCGGGGGTCGCGGGAGACTTCTCCTACGTCTCCA
CCGCCGGCGGCGCCTTCCTGGAATGGTTGGAAGGCAAGGCCCTGCCGGGTGTCGAGGCCCTTCGGCGCTGA

Upstream 100 bases:

>100_bases
TGCTGTCCTGGTATGACAACGAGTGGGGCTTCTCCAACCGCATGGCCGACACTGCAGTCGCCATGGGCAAGCTCGGTTGA
GCGGGGAAGGGGGCGCCAAG

Downstream 100 bases:

>100_bases
AAAAAGACCGCCCGGATGACAAAATGTCGTCCGGGCGTTTGTTTGCTGCATCGCAGCGGGTTCATTTGCTGCGCTGCAAT
GGTATGATGTCTCGCTGGCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTAFRTLDDADLADKRVLVRVDLNVPMESGRVTDETRLKAILPTIRTITDKGGKAVLLAHFGRPKGRDESQSLAPVAKAL
EGQLGRKVAFASDCVGEEASSAISRLAAGEVIVLENTRFHAGEEKNAPDFVEALASLGDIYVNDAFSAAHRAHASTEGLA
RKLPAYAGRSMESELAALTKALEAPVRPVLAVVGGSKVSSKLELLGNLVKKVDILVIAGGMANTFLAALGKKVGKSLCEH
DLADTAREILEKAKAAGCEIVLPVDAVVAKEFKANAANRVVSVDEVGDDEMILDAGPETVAVVAQKLDGAKTVVWNGPFG
AFEMTPFDAATVAVARDVGKRTRAGTLLSVAGGGDTVAALNHAGVAGDFSYVSTAGGAFLEWLEGKALPGVEALRR

Sequences:

>Translated_396_residues
MTAFRTLDDADLADKRVLVRVDLNVPMESGRVTDETRLKAILPTIRTITDKGGKAVLLAHFGRPKGRDESQSLAPVAKAL
EGQLGRKVAFASDCVGEEASSAISRLAAGEVIVLENTRFHAGEEKNAPDFVEALASLGDIYVNDAFSAAHRAHASTEGLA
RKLPAYAGRSMESELAALTKALEAPVRPVLAVVGGSKVSSKLELLGNLVKKVDILVIAGGMANTFLAALGKKVGKSLCEH
DLADTAREILEKAKAAGCEIVLPVDAVVAKEFKANAANRVVSVDEVGDDEMILDAGPETVAVVAQKLDGAKTVVWNGPFG
AFEMTPFDAATVAVARDVGKRTRAGTLLSVAGGGDTVAALNHAGVAGDFSYVSTAGGAFLEWLEGKALPGVEALRR
>Mature_395_residues
TAFRTLDDADLADKRVLVRVDLNVPMESGRVTDETRLKAILPTIRTITDKGGKAVLLAHFGRPKGRDESQSLAPVAKALE
GQLGRKVAFASDCVGEEASSAISRLAAGEVIVLENTRFHAGEEKNAPDFVEALASLGDIYVNDAFSAAHRAHASTEGLAR
KLPAYAGRSMESELAALTKALEAPVRPVLAVVGGSKVSSKLELLGNLVKKVDILVIAGGMANTFLAALGKKVGKSLCEHD
LADTAREILEKAKAAGCEIVLPVDAVVAKEFKANAANRVVSVDEVGDDEMILDAGPETVAVVAQKLDGAKTVVWNGPFGA
FEMTPFDAATVAVARDVGKRTRAGTLLSVAGGGDTVAALNHAGVAGDFSYVSTAGGAFLEWLEGKALPGVEALRR

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=44.390243902439, Blast_Score=304, Evalue=9e-83,
Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=43.4146341463415, Blast_Score=301, Evalue=9e-82,
Organism=Escherichia coli, GI1789294, Length=393, Percent_Identity=44.2748091603053, Blast_Score=298, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=44.963144963145, Blast_Score=321, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=44.3099273607748, Blast_Score=308, Evalue=8e-85,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=42.4019607843137, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=40.7317073170732, Blast_Score=273, Evalue=1e-73,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41276; Mature: 41144

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFRTLDDADLADKRVLVRVDLNVPMESGRVTDETRLKAILPTIRTITDKGGKAVLLAH
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
FGRPKGRDESQSLAPVAKALEGQLGRKVAFASDCVGEEASSAISRLAAGEVIVLENTRFH
CCCCCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHCCHHHHHHHHHHHCCCEEEEECCEEC
AGEEKNAPDFVEALASLGDIYVNDAFSAAHRAHASTEGLARKLPAYAGRSMESELAALTK
CCCCCCCCHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALEAPVRPVLAVVGGSKVSSKLELLGNLVKKVDILVIAGGMANTFLAALGKKVGKSLCEH
HHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHH
DLADTAREILEKAKAAGCEIVLPVDAVVAKEFKANAANRVVSVDEVGDDEMILDAGPETV
HHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCHHHCEEECCCCCCCCEEEECCCHHH
AVVAQKLDGAKTVVWNGPFGAFEMTPFDAATVAVARDVGKRTRAGTLLSVAGGGDTVAAL
HHHHHHCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEHH
NHAGVAGDFSYVSTAGGAFLEWLEGKALPGVEALRR
HCCCCCCCCHHHHHCCHHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
TAFRTLDDADLADKRVLVRVDLNVPMESGRVTDETRLKAILPTIRTITDKGGKAVLLAH
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
FGRPKGRDESQSLAPVAKALEGQLGRKVAFASDCVGEEASSAISRLAAGEVIVLENTRFH
CCCCCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHCCHHHHHHHHHHHCCCEEEEECCEEC
AGEEKNAPDFVEALASLGDIYVNDAFSAAHRAHASTEGLARKLPAYAGRSMESELAALTK
CCCCCCCCHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALEAPVRPVLAVVGGSKVSSKLELLGNLVKKVDILVIAGGMANTFLAALGKKVGKSLCEH
HHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHH
DLADTAREILEKAKAAGCEIVLPVDAVVAKEFKANAANRVVSVDEVGDDEMILDAGPETV
HHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCHHHCEEECCCCCCCCEEEECCCHHH
AVVAQKLDGAKTVVWNGPFGAFEMTPFDAATVAVARDVGKRTRAGTLLSVAGGGDTVAAL
HHHHHHCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEHH
NHAGVAGDFSYVSTAGGAFLEWLEGKALPGVEALRR
HCCCCCCCCHHHHHCCHHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7928974 [H]