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The map label for this gene is cca [H]

Identifier: 154246887

GI number: 154246887

Start: 3278446

End: 3279666

Strand: Direct

Name: cca [H]

Synonym: Xaut_2952

Alternate gene names: 154246887

Gene position: 3278446-3279666 (Clockwise)

Preceding gene: 154246886

Following gene: 154246892

Centisome position: 61.75

GC content: 73.22

Gene sequence:

>1221_bases
ATGAGCCTGTCCATCGTCGGCGCGCCGTTCTGGCGCACGCCGGGCCTCATGGCCGTGCTGGCCGCGCTCAATGCGGACGG
GGAGGAGGCGCGCGTGGTGGGCGGCGCGGTGCGCAACACCCTCCTCGGCCTTCCGGTCTCGGACGTGGACATCGCCACCA
CCGCCGTTCCCGAGGTGGTGGCCGAGCGGGCGCGCAAGGCCGGCCTGAAGCCGGTGCCCACCGGCGTCGAGCATGGCACC
GTGACCGTGGTGGCGGACCACCACGCCTTTGAGGTCACCACCCTGCGCGAGGACATGGAAACCGATGGCCGCCGCGCGGT
GGTGCGCTTCGGCCGCAGCTGGCTGCATGATGCCGAGCGACGGGACTTCACCCTCAACGCCCTCTATGCGACGGCTGACG
GGGCGGTGGTGGACCTGGTGGGCGGCCTGTCGGATCTCACCGCGCGGCGCATCCGCTTCATCGGCGACCCGCAGGCGCGC
ATCCGCGAGGATTACCTGCGCATCCTGCGCCTGTTCCGCTTCCATGCGGCCTATGGCGCGGGGCCGGTAGACAAGGACGC
CTTCATCGCCAGCGTGCGCCGGCGGGCCGGCCTTCTCACCCTGTCGCGGGAGCGGGTGCGGACCGAGATGCTGAAGCTGC
TGGTGGCGCCGGGCGCTGCGGCGACCCTCGCCGTCATGAGCGATGCCGGCCTCGTCCAGCCGCTGATCGCCGGCATCGCC
GACGTGGGGGCGTTCACGCGGCTCGCGGCGCTGGAGCGGGACTGGCAGGCGAAGCCCGACGCCATCCGTCGGCTCGCGGC
CCTCGCCGTGCGCGTTAGTGATGACGCGGCGCGCCTGCGCGACCGGTTGCGGCTGTCCAATGCGGAGGCCCGCCGGCTCG
CCGCCCTGTCGGCGACCGTCTCGGACCTGCCCGATGCCCAGGCCGTGCGGGCATTCCTTTACCGGAACGGCGTGGATGCG
GCGCTGGATCTGGCCTTGCTCGCCGCCGCCCATGGCCGGGACGGTATGGAGCGAGTGGCGGCTATGGCGGCCTCCTGGAC
TACACCGCGCCTGCCATTCGCCGCGGCCGACCTGATGGCGTTGGGGCTCATCCCCGGCCGCGCGCTCGGCGCGGCCCTGG
CCCGGGCCGAGGCACGATGGATCGCCGCCGACTTTCCGACGGATCCGGACGTGGTCGCCGGCCTCCTCGCGGAGGCGGTC
AAGGCCTCGTCTGCCGCCTGA

Upstream 100 bases:

>100_bases
CCGGCCTGTTCCTGTTCGAGCACGGCAATGTGGCCCCGCCCGGCAGCCGCTACGTGCCCGCCCAGGTGAAGGACGGCGTG
TTCGTGCCGGGGCGTTTCGA

Downstream 100 bases:

>100_bases
GGCGTCAGTCCTCGTCTTCTTCCTCTTCGTCTTCTTCCTCTTCTTCATCCTCAAAATCGGCGACTTCGAGCTTCTCGAGG
GCGATGACCGGAAGGGTGTG

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSLSIVGAPFWRTPGLMAVLAALNADGEEARVVGGAVRNTLLGLPVSDVDIATTAVPEVVAERARKAGLKPVPTGVEHGT
VTVVADHHAFEVTTLREDMETDGRRAVVRFGRSWLHDAERRDFTLNALYATADGAVVDLVGGLSDLTARRIRFIGDPQAR
IREDYLRILRLFRFHAAYGAGPVDKDAFIASVRRRAGLLTLSRERVRTEMLKLLVAPGAAATLAVMSDAGLVQPLIAGIA
DVGAFTRLAALERDWQAKPDAIRRLAALAVRVSDDAARLRDRLRLSNAEARRLAALSATVSDLPDAQAVRAFLYRNGVDA
ALDLALLAAAHGRDGMERVAAMAASWTTPRLPFAAADLMALGLIPGRALGAALARAEARWIAADFPTDPDVVAGLLAEAV
KASSAA

Sequences:

>Translated_406_residues
MSLSIVGAPFWRTPGLMAVLAALNADGEEARVVGGAVRNTLLGLPVSDVDIATTAVPEVVAERARKAGLKPVPTGVEHGT
VTVVADHHAFEVTTLREDMETDGRRAVVRFGRSWLHDAERRDFTLNALYATADGAVVDLVGGLSDLTARRIRFIGDPQAR
IREDYLRILRLFRFHAAYGAGPVDKDAFIASVRRRAGLLTLSRERVRTEMLKLLVAPGAAATLAVMSDAGLVQPLIAGIA
DVGAFTRLAALERDWQAKPDAIRRLAALAVRVSDDAARLRDRLRLSNAEARRLAALSATVSDLPDAQAVRAFLYRNGVDA
ALDLALLAAAHGRDGMERVAAMAASWTTPRLPFAAADLMALGLIPGRALGAALARAEARWIAADFPTDPDVVAGLLAEAV
KASSAA
>Mature_405_residues
SLSIVGAPFWRTPGLMAVLAALNADGEEARVVGGAVRNTLLGLPVSDVDIATTAVPEVVAERARKAGLKPVPTGVEHGTV
TVVADHHAFEVTTLREDMETDGRRAVVRFGRSWLHDAERRDFTLNALYATADGAVVDLVGGLSDLTARRIRFIGDPQARI
REDYLRILRLFRFHAAYGAGPVDKDAFIASVRRRAGLLTLSRERVRTEMLKLLVAPGAAATLAVMSDAGLVQPLIAGIAD
VGAFTRLAALERDWQAKPDAIRRLAALAVRVSDDAARLRDRLRLSNAEARRLAALSATVSDLPDAQAVRAFLYRNGVDAA
LDLALLAAAHGRDGMERVAAMAASWTTPRLPFAAADLMALGLIPGRALGAALARAEARWIAADFPTDPDVVAGLLAEAVK
ASSAA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=400, Percent_Identity=30.75, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI87081691, Length=236, Percent_Identity=37.2881355932203, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789436, Length=213, Percent_Identity=30.0469483568075, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71995920, Length=389, Percent_Identity=31.1053984575836, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI281360112, Length=408, Percent_Identity=30.6372549019608, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI21357337, Length=408, Percent_Identity=30.6372549019608, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 43131; Mature: 43000

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSIVGAPFWRTPGLMAVLAALNADGEEARVVGGAVRNTLLGLPVSDVDIATTAVPEVV
CCCEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AERARKAGLKPVPTGVEHGTVTVVADHHAFEVTTLREDMETDGRRAVVRFGRSWLHDAER
HHHHHHCCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
RDFTLNALYATADGAVVDLVGGLSDLTARRIRFIGDPQARIREDYLRILRLFRFHAAYGA
CCEEEEEEEEECCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
GPVDKDAFIASVRRRAGLLTLSRERVRTEMLKLLVAPGAAATLAVMSDAGLVQPLIAGIA
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
DVGAFTRLAALERDWQAKPDAIRRLAALAVRVSDDAARLRDRLRLSNAEARRLAALSATV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
SDLPDAQAVRAFLYRNGVDAALDLALLAAAHGRDGMERVAAMAASWTTPRLPFAAADLMA
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
LGLIPGRALGAALARAEARWIAADFPTDPDVVAGLLAEAVKASSAA
HHCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLSIVGAPFWRTPGLMAVLAALNADGEEARVVGGAVRNTLLGLPVSDVDIATTAVPEVV
CCEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AERARKAGLKPVPTGVEHGTVTVVADHHAFEVTTLREDMETDGRRAVVRFGRSWLHDAER
HHHHHHCCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
RDFTLNALYATADGAVVDLVGGLSDLTARRIRFIGDPQARIREDYLRILRLFRFHAAYGA
CCEEEEEEEEECCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
GPVDKDAFIASVRRRAGLLTLSRERVRTEMLKLLVAPGAAATLAVMSDAGLVQPLIAGIA
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
DVGAFTRLAALERDWQAKPDAIRRLAALAVRVSDDAARLRDRLRLSNAEARRLAALSATV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
SDLPDAQAVRAFLYRNGVDAALDLALLAAAHGRDGMERVAAMAASWTTPRLPFAAADLMA
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
LGLIPGRALGAALARAEARWIAADFPTDPDVVAGLLAEAVKASSAA
HHCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA