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The map label for this gene is potH [H]

Identifier: 154246877

GI number: 154246877

Start: 3267424

End: 3268341

Strand: Direct

Name: potH [H]

Synonym: Xaut_2942

Alternate gene names: 154246877

Gene position: 3267424-3268341 (Clockwise)

Preceding gene: 154246876

Following gene: 154246878

Centisome position: 61.55

GC content: 64.38

Gene sequence:

>918_bases
ATGGCGATGGCCGGGGAGAAGGGACGGGGCCGCTGGTTCGTGATCGCGGTGCCCTATCTGTGGCTCCTCGCGCTGTTCCT
TGCGCCCTTCCTCATCGTATTCAAGATCTCGCTGTCGCAGGACGTGGTGGCCCAGCCGCCCTACGCGCCGACCCTCGACC
TGGCGCAGGGCTGGGCGGGCTTCACCGAATTCCTGTCGGGCCTGTCCTTCGCGAACTATGCCTATGTGCTGGATTTTTCG
AATGAGTTCCTGGAGGCTTACGGCTCCAGCCTGATCATCGCCTCGATCTCCACCGTGCTTCTGCTGCTGGTGGGGTACCC
AGTCGCCTACGCCATGGCGCGGGCGCCGCGCTCCATCCAGCCGACGCTGCTGATGCTGGTGATCCTGCCGTTCTGGACCT
CCTTCCTCATCCGCGTCTATGCGTGGATCGGCATCCTGTCGCAGGAAGGGCTGCTCAATCAGGCCCTGATGGCGCTGGGG
GTGATCCACACGCCGCTGGAGATCCTGAACACCAACCTCGCCGTCTATATCGGCATCGTCTATTCCTACCTGCCTTTCAT
GGTGCTGCCGCTTTACGCGGCACTGGAAAAGCTCGACCTCACGCTGCTGGAGGCCGCCGCCGATCTCGGCTGCCCGCCGG
CCAAGGCGTTCTGGACCATCACCTTTCCGCTCTCCCTGCCGGGCGTGGCGGCCGGCGCCCTGCTGTGCTTCATCCCGGCG
GTGGGAGAGTTCGTCATTCCCGACCTCCTGGGCGGCTCGGACACGCTGATGATCGGCAAGTCGCTATGGACCGAGTTCAC
CGTGAACCGGTCCTGGACGGTCTCGTCCGCGGTGGCGGTGCTGCTGCTGCTCGTGCTGGTGATCCCCATCGTGATCTACC
AGAACATCCAGCAGCGCGAGCTGGAGGCGGGCAAATGA

Upstream 100 bases:

>100_bases
TTGAGCGTGACCCGCCTGGCATCGGTGGCAGGCCGGGGCCGATGGTATGAGTTGAGGGTTCTGACGCGTTTTCATCGGGC
GGAAGCGCCGGGAGGCAACC

Downstream 100 bases:

>100_bases
GAAAAGGCCTTACCTGGTTCAACATCACGTCCATCGTGCTGGGCTTCGCCTTCCTCTACATCCCCATCCTGTTGCTGGTG
ATCTATTCCTTCAACGCCTC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAMAGEKGRGRWFVIAVPYLWLLALFLAPFLIVFKISLSQDVVAQPPYAPTLDLAQGWAGFTEFLSGLSFANYAYVLDFS
NEFLEAYGSSLIIASISTVLLLLVGYPVAYAMARAPRSIQPTLLMLVILPFWTSFLIRVYAWIGILSQEGLLNQALMALG
VIHTPLEILNTNLAVYIGIVYSYLPFMVLPLYAALEKLDLTLLEAAADLGCPPAKAFWTITFPLSLPGVAAGALLCFIPA
VGEFVIPDLLGGSDTLMIGKSLWTEFTVNRSWTVSSAVAVLLLLVLVIPIVIYQNIQQRELEAGK

Sequences:

>Translated_305_residues
MAMAGEKGRGRWFVIAVPYLWLLALFLAPFLIVFKISLSQDVVAQPPYAPTLDLAQGWAGFTEFLSGLSFANYAYVLDFS
NEFLEAYGSSLIIASISTVLLLLVGYPVAYAMARAPRSIQPTLLMLVILPFWTSFLIRVYAWIGILSQEGLLNQALMALG
VIHTPLEILNTNLAVYIGIVYSYLPFMVLPLYAALEKLDLTLLEAAADLGCPPAKAFWTITFPLSLPGVAAGALLCFIPA
VGEFVIPDLLGGSDTLMIGKSLWTEFTVNRSWTVSSAVAVLLLLVLVIPIVIYQNIQQRELEAGK
>Mature_304_residues
AMAGEKGRGRWFVIAVPYLWLLALFLAPFLIVFKISLSQDVVAQPPYAPTLDLAQGWAGFTEFLSGLSFANYAYVLDFSN
EFLEAYGSSLIIASISTVLLLLVGYPVAYAMARAPRSIQPTLLMLVILPFWTSFLIRVYAWIGILSQEGLLNQALMALGV
IHTPLEILNTNLAVYIGIVYSYLPFMVLPLYAALEKLDLTLLEAAADLGCPPAKAFWTITFPLSLPGVAAGALLCFIPAV
GEFVIPDLLGGSDTLMIGKSLWTEFTVNRSWTVSSAVAVLLLLVLVIPIVIYQNIQQRELEAGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=296, Percent_Identity=53.7162162162162, Blast_Score=274, Evalue=6e-75,
Organism=Escherichia coli, GI1787369, Length=240, Percent_Identity=42.5, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1787713, Length=214, Percent_Identity=30.8411214953271, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33298; Mature: 33167

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMAGEKGRGRWFVIAVPYLWLLALFLAPFLIVFKISLSQDVVAQPPYAPTLDLAQGWAG
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
FTEFLSGLSFANYAYVLDFSNEFLEAYGSSLIIASISTVLLLLVGYPVAYAMARAPRSIQ
HHHHHHCCCCCCEEEEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTLLMLVILPFWTSFLIRVYAWIGILSQEGLLNQALMALGVIHTPLEILNTNLAVYIGIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
YSYLPFMVLPLYAALEKLDLTLLEAAADLGCPPAKAFWTITFPLSLPGVAAGALLCFIPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
VGEFVIPDLLGGSDTLMIGKSLWTEFTVNRSWTVSSAVAVLLLLVLVIPIVIYQNIQQRE
HHHHHHHHHCCCCCCEEEHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAGK
HCCCC
>Mature Secondary Structure 
AMAGEKGRGRWFVIAVPYLWLLALFLAPFLIVFKISLSQDVVAQPPYAPTLDLAQGWAG
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
FTEFLSGLSFANYAYVLDFSNEFLEAYGSSLIIASISTVLLLLVGYPVAYAMARAPRSIQ
HHHHHHCCCCCCEEEEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTLLMLVILPFWTSFLIRVYAWIGILSQEGLLNQALMALGVIHTPLEILNTNLAVYIGIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
YSYLPFMVLPLYAALEKLDLTLLEAAADLGCPPAKAFWTITFPLSLPGVAAGALLCFIPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
VGEFVIPDLLGGSDTLMIGKSLWTEFTVNRSWTVSSAVAVLLLLVLVIPIVIYQNIQQRE
HHHHHHHHHCCCCCCEEEHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAGK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]