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The map label for this gene is metF [H]

Identifier: 154246770

GI number: 154246770

Start: 3154999

End: 3155952

Strand: Direct

Name: metF [H]

Synonym: Xaut_2831

Alternate gene names: 154246770

Gene position: 3154999-3155952 (Clockwise)

Preceding gene: 154246769

Following gene: 154246771

Centisome position: 59.43

GC content: 67.71

Gene sequence:

>954_bases
ATGACCACGCCCGCCCTTGCTCCTGCTCCCGCCCCGGTCCGGGCGAGCCGGGAGATGGACCGGCCGCCGGTGAAGGTCTC
CTTCGAGTTCTTCCCGCCGAAGTCGCCAGAGATGGAAAAGACCCTGTGGTCGTCCATCGCCCGCCTCGCGCCGCTGTCGC
CTGATTTCGTGTCGGTCACCTACGGCGCCGGCGGCTCCACCCGCGAGCGCACCCACGCCACCGTGGAGCGCATCGCCAAG
GAGACCGACCTTGCCCCCGCCGCCCACCTCACCTGCGTGGCCGCGACCCGGGACGAGATCGACGCCGTGGTGCGCGCCTA
TGGCGAGGCGGGCGTGAAGCATATCGTGGCCCTGCGCGGCGATCCGGTGACCGGGGTGGGCACGCGCTATGAGCCCCATC
CCGGCGGCTATGCCTATGCCAATGACCTCGTGGCCGGCATCAAGCGCATCGGCGATTTCGAGGTGTCGGTCTCCGCCTAT
CCGGAAAAGCACCCGGAGAGTGCGAGCCTTGATGCCGACATTGATGTCCTGAAGGCCAAGGTGGATGCGGGCGCCACCCG
CGCCATCACCCAGTTCTTCTTCGAGAACGACGTGTATTTCCGCTATCTGGACAAGGTGCGGGCGCGGGGCATCGACATTC
CCATCGTGCCGGGCATCCTGCCGGTGACCAACTTCAAGCAGGCCTCCAGCTTTGCTACCCGCACCGGCGCCAGCGTGCCG
GCCTGGCTGCTGCGGCGGTTCGAGGGGCTCGACGACGACGTGGAGACTCGCAAGCTCATCGCCGCCGCCGTGGCCGCCGA
GCAGGTGACGGACCTCGTTGATCGCGGTGTCACCGATATCCATTTCTATACGCTGAACCGCGCCGACCTCGTCTACGCCA
TCTGCCACCTGCTGGGGTTGCGGCCCAAGGTGGCGGGCGCGCTGGCGGATGCGCCAAAGGCTGTCGCCATCTGA

Upstream 100 bases:

>100_bases
ACGGCAAGGCGGGGGACGGGCTCACCGTTTCGCTCTGGCTGGCGCGCGACCCACGCGTGCTCATGGCCGAGGCCGACCTT
GCCGACAGGGAGATTGCCTG

Downstream 100 bases:

>100_bases
ACGCCGGAAGCCCTCTCCCGCTCGCGGGGGAGGGTTGGTAGGGGGCCACAGTCCCATCGGCGCCGGAAAAATTGGCGACG
ATGTCGTGTTTTCGTCCGGC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTTPALAPAPAPVRASREMDRPPVKVSFEFFPPKSPEMEKTLWSSIARLAPLSPDFVSVTYGAGGSTRERTHATVERIAK
ETDLAPAAHLTCVAATRDEIDAVVRAYGEAGVKHIVALRGDPVTGVGTRYEPHPGGYAYANDLVAGIKRIGDFEVSVSAY
PEKHPESASLDADIDVLKAKVDAGATRAITQFFFENDVYFRYLDKVRARGIDIPIVPGILPVTNFKQASSFATRTGASVP
AWLLRRFEGLDDDVETRKLIAAAVAAEQVTDLVDRGVTDIHFYTLNRADLVYAICHLLGLRPKVAGALADAPKAVAI

Sequences:

>Translated_317_residues
MTTPALAPAPAPVRASREMDRPPVKVSFEFFPPKSPEMEKTLWSSIARLAPLSPDFVSVTYGAGGSTRERTHATVERIAK
ETDLAPAAHLTCVAATRDEIDAVVRAYGEAGVKHIVALRGDPVTGVGTRYEPHPGGYAYANDLVAGIKRIGDFEVSVSAY
PEKHPESASLDADIDVLKAKVDAGATRAITQFFFENDVYFRYLDKVRARGIDIPIVPGILPVTNFKQASSFATRTGASVP
AWLLRRFEGLDDDVETRKLIAAAVAAEQVTDLVDRGVTDIHFYTLNRADLVYAICHLLGLRPKVAGALADAPKAVAI
>Mature_316_residues
TTPALAPAPAPVRASREMDRPPVKVSFEFFPPKSPEMEKTLWSSIARLAPLSPDFVSVTYGAGGSTRERTHATVERIAKE
TDLAPAAHLTCVAATRDEIDAVVRAYGEAGVKHIVALRGDPVTGVGTRYEPHPGGYAYANDLVAGIKRIGDFEVSVSAYP
EKHPESASLDADIDVLKAKVDAGATRAITQFFFENDVYFRYLDKVRARGIDIPIVPGILPVTNFKQASSFATRTGASVPA
WLLRRFEGLDDDVETRKLIAAAVAAEQVTDLVDRGVTDIHFYTLNRADLVYAICHLLGLRPKVAGALADAPKAVAI

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=327, Percent_Identity=33.0275229357798, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1790377, Length=279, Percent_Identity=54.8387096774194, Blast_Score=317, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI25147436, Length=300, Percent_Identity=28.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25147441, Length=300, Percent_Identity=28.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321313, Length=284, Percent_Identity=31.6901408450704, Blast_Score=142, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6325234, Length=295, Percent_Identity=35.2542372881356, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24662658, Length=275, Percent_Identity=27.6363636363636, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34196; Mature: 34065

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPALAPAPAPVRASREMDRPPVKVSFEFFPPKSPEMEKTLWSSIARLAPLSPDFVSVT
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
YGAGGSTRERTHATVERIAKETDLAPAAHLTCVAATRDEIDAVVRAYGEAGVKHIVALRG
ECCCCCCHHHHHHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHEEEEECC
DPVTGVGTRYEPHPGGYAYANDLVAGIKRIGDFEVSVSAYPEKHPESASLDADIDVLKAK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHH
VDAGATRAITQFFFENDVYFRYLDKVRARGIDIPIVPGILPVTNFKQASSFATRTGASVP
HCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCH
AWLLRRFEGLDDDVETRKLIAAAVAAEQVTDLVDRGVTDIHFYTLNRADLVYAICHLLGL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHCC
RPKVAGALADAPKAVAI
CHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTPALAPAPAPVRASREMDRPPVKVSFEFFPPKSPEMEKTLWSSIARLAPLSPDFVSVT
CCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
YGAGGSTRERTHATVERIAKETDLAPAAHLTCVAATRDEIDAVVRAYGEAGVKHIVALRG
ECCCCCCHHHHHHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHEEEEECC
DPVTGVGTRYEPHPGGYAYANDLVAGIKRIGDFEVSVSAYPEKHPESASLDADIDVLKAK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHH
VDAGATRAITQFFFENDVYFRYLDKVRARGIDIPIVPGILPVTNFKQASSFATRTGASVP
HCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCH
AWLLRRFEGLDDDVETRKLIAAAVAAEQVTDLVDRGVTDIHFYTLNRADLVYAICHLLGL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHCC
RPKVAGALADAPKAVAI
CHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA