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The map label for this gene is bepF [H]

Identifier: 154246765

GI number: 154246765

Start: 3146829

End: 3148019

Strand: Direct

Name: bepF [H]

Synonym: Xaut_2826

Alternate gene names: 154246765

Gene position: 3146829-3148019 (Clockwise)

Preceding gene: 154246764

Following gene: 154246766

Centisome position: 59.27

GC content: 72.38

Gene sequence:

>1191_bases
ATGCCCGTCGCTGCCCCCGACTCCCGTCTGCCTTCCGGTTCGCCGCGCCTTTTGCGCGCAGGCCTTCTCGCCTCTTCCCT
CGCCGCCGTCTCCGTCCTTCTGGCTGCCTGCGGAGAGTCCGCCCCGGCCCAGACCGAGGCGGATGCGCCTCAGGTGGTGG
CCCGGCCGGTGCAGGTTACCACCGTGGCCTTCAAGCCGCGCGAGACCGAGAAGACCTTCGTCGGCGTGGTCCGGGCGCGG
CGGGAGATCGACCTCGCCTTCCGCGTGGGCGGCAAGATGGTGCAGCGGAAGGTGGAGGTGGGCGACCTCGTGGCGCCGGG
CGACGTGGTGGCCCGCATGGATCCCGAAGACCTGCGGCTCGAACTGGAAAGCGCGCGCGCCGAGCTTGCCGCTGCCACCG
CCAACCTCAACCAGACCTCCGCCGAGGACGGCCGCACCCGCTCGCTCACGGCAAAGGGCTATTCCTCGGCCGCCGACCTC
GACCGCAAGTCCCTCGCCAAGGAAGAGGCAGTGGGGCGCATAGAGCGGGCCAAGCGATCGCTGGAGCTGGCCGAGAACCG
GCTCTCCTACGCCGAACTGGTAGCCGATGCCGCCGGCATGGTGGTGGCCACCGCCGCCGAGCCGGGACAGGTGGTGTCCT
CCGGCCAGACCATCGTCCGCGTGGCGCGGCTCGATGAGAAGGAGGCCGTGGTCTCCCTGCCCGAGACGGCGCTGGCCGAT
GCCCGCGCTGCCGACGCCTTCGTGACCCTGTGGGCGGAGCCCGGCCGGCGCATTCCCGCCCACCTGCGCGAGCTTTCGCC
CCAGGCCGATGCCACCTCGCGCACCTATCCCGCCCGCTTCACCCTCGAAGGGGCAGACGCCAGCGTCGCCCTTGGCATGA
CCGCCACCGTCACCCTGCGCCCCAAGGACCAGCAGGCGGTGGCGCGGCTGCCGCTCTCCGCCGTCATCGATCGGGGCCAT
GGCCCGCAGGTGTTCGTGGTGGACGGGGCGAGCCACACCCTGGCCGCACGGCCGGTCACCATCGCCGCCTATACGGAGGA
CCAGGTGCTGGTGGCCGCCGGCGTCGCGCCCGGAGATGAGGTGGTGACGCTGGGCGTGCAGACGCTGCTGCCGGGCCAGA
AGGTGCGCACCGCCAAGCTCCCCGGCTCCACCGACATGGCCGCCGCCGGCCTCAAGGAGATCCGGCCGTGA

Upstream 100 bases:

>100_bases
TGAATGGGGGTGATCCGCGCGCCTTCGTGCGCCTTAAAAGTGATGAAAGATGAATTTCGTCAGCGTTGAGCTGTTACTCG
ACATTTGACAGGACCGTGCC

Downstream 100 bases:

>100_bases
GCGCTGCTGACAAGCCCCCCGCCACTCAGCCTTCCGCCCACCAGCCTCCCGGGAGCGCGCCGGATGACGCCGGCTCCGGC
TTCAACCTGTCGCGCTGGGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MPVAAPDSRLPSGSPRLLRAGLLASSLAAVSVLLAACGESAPAQTEADAPQVVARPVQVTTVAFKPRETEKTFVGVVRAR
REIDLAFRVGGKMVQRKVEVGDLVAPGDVVARMDPEDLRLELESARAELAAATANLNQTSAEDGRTRSLTAKGYSSAADL
DRKSLAKEEAVGRIERAKRSLELAENRLSYAELVADAAGMVVATAAEPGQVVSSGQTIVRVARLDEKEAVVSLPETALAD
ARAADAFVTLWAEPGRRIPAHLRELSPQADATSRTYPARFTLEGADASVALGMTATVTLRPKDQQAVARLPLSAVIDRGH
GPQVFVVDGASHTLAARPVTIAAYTEDQVLVAAGVAPGDEVVTLGVQTLLPGQKVRTAKLPGSTDMAAAGLKEIRP

Sequences:

>Translated_396_residues
MPVAAPDSRLPSGSPRLLRAGLLASSLAAVSVLLAACGESAPAQTEADAPQVVARPVQVTTVAFKPRETEKTFVGVVRAR
REIDLAFRVGGKMVQRKVEVGDLVAPGDVVARMDPEDLRLELESARAELAAATANLNQTSAEDGRTRSLTAKGYSSAADL
DRKSLAKEEAVGRIERAKRSLELAENRLSYAELVADAAGMVVATAAEPGQVVSSGQTIVRVARLDEKEAVVSLPETALAD
ARAADAFVTLWAEPGRRIPAHLRELSPQADATSRTYPARFTLEGADASVALGMTATVTLRPKDQQAVARLPLSAVIDRGH
GPQVFVVDGASHTLAARPVTIAAYTEDQVLVAAGVAPGDEVVTLGVQTLLPGQKVRTAKLPGSTDMAAAGLKEIRP
>Mature_395_residues
PVAAPDSRLPSGSPRLLRAGLLASSLAAVSVLLAACGESAPAQTEADAPQVVARPVQVTTVAFKPRETEKTFVGVVRARR
EIDLAFRVGGKMVQRKVEVGDLVAPGDVVARMDPEDLRLELESARAELAAATANLNQTSAEDGRTRSLTAKGYSSAADLD
RKSLAKEEAVGRIERAKRSLELAENRLSYAELVADAAGMVVATAAEPGQVVSSGQTIVRVARLDEKEAVVSLPETALADA
RAADAFVTLWAEPGRRIPAHLRELSPQADATSRTYPARFTLEGADASVALGMTATVTLRPKDQQAVARLPLSAVIDRGHG
PQVFVVDGASHTLAARPVTIAAYTEDQVLVAAGVAPGDEVVTLGVQTLLPGQKVRTAKLPGSTDMAAAGLKEIRP

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=25, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082036, Length=329, Percent_Identity=24.6200607902736, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081796, Length=324, Percent_Identity=25.6172839506173, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789665, Length=397, Percent_Identity=25.1889168765743, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41433; Mature: 41301

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAAPDSRLPSGSPRLLRAGLLASSLAAVSVLLAACGESAPAQTEADAPQVVARPVQVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEE
TVAFKPRETEKTFVGVVRARREIDLAFRVGGKMVQRKVEVGDLVAPGDVVARMDPEDLRL
EEEECCCCCHHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHCCEECCCCEEEECCHHHHHE
ELESARAELAAATANLNQTSAEDGRTRSLTAKGYSSAADLDRKSLAKEEAVGRIERAKRS
EHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHH
LELAENRLSYAELVADAAGMVVATAAEPGQVVSSGQTIVRVARLDEKEAVVSLPETALAD
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCEEEEECCCCCHHHEECCHHHHHH
ARAADAFVTLWAEPGRRIPAHLRELSPQADATSRTYPARFTLEGADASVALGMTATVTLR
HHHCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEECEEEEEEEC
PKDQQAVARLPLSAVIDRGHGPQVFVVDGASHTLAARPVTIAAYTEDQVLVAAGVAPGDE
CCCHHHHHHCCHHHHHCCCCCCEEEEECCCCCEEEECCEEEEEECCCCEEEEECCCCCCC
VVTLGVQTLLPGQKVRTAKLPGSTDMAAAGLKEIRP
EEEEEHHHHCCCCCEEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
PVAAPDSRLPSGSPRLLRAGLLASSLAAVSVLLAACGESAPAQTEADAPQVVARPVQVT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEE
TVAFKPRETEKTFVGVVRARREIDLAFRVGGKMVQRKVEVGDLVAPGDVVARMDPEDLRL
EEEECCCCCHHHHHHHHHHHHHHEEHHHHCCHHHHHHHHHCCEECCCCEEEECCHHHHHE
ELESARAELAAATANLNQTSAEDGRTRSLTAKGYSSAADLDRKSLAKEEAVGRIERAKRS
EHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHH
LELAENRLSYAELVADAAGMVVATAAEPGQVVSSGQTIVRVARLDEKEAVVSLPETALAD
HHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCEEEEECCCCCHHHEECCHHHHHH
ARAADAFVTLWAEPGRRIPAHLRELSPQADATSRTYPARFTLEGADASVALGMTATVTLR
HHHCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEECEEEEEEEC
PKDQQAVARLPLSAVIDRGHGPQVFVVDGASHTLAARPVTIAAYTEDQVLVAAGVAPGDE
CCCHHHHHHCCHHHHHCCCCCCEEEEECCCCCEEEECCEEEEEECCCCEEEEECCCCCCC
VVTLGVQTLLPGQKVRTAKLPGSTDMAAAGLKEIRP
EEEEEHHHHCCCCCEEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]