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The map label for this gene is sdhA [C]

Identifier: 154246740

GI number: 154246740

Start: 3120200

End: 3121936

Strand: Direct

Name: sdhA [C]

Synonym: Xaut_2801

Alternate gene names: 154246740

Gene position: 3120200-3121936 (Clockwise)

Preceding gene: 154246739

Following gene: 154246741

Centisome position: 58.77

GC content: 64.48

Gene sequence:

>1737_bases
ATGAGCGAGATCAAGACCGAGATCGTCGAAACCGACATCCTCGTCATCGGCGGCGGCACCGGCGGGCCGATGGCCGCCAT
CAAGGCCAAGGAGAAGAATCCCGGCCTGAAGGTGGTGCTGATGGAGAAGGCCAATGTGAAGCGCTCCGGCGCGGTCTCCA
TGGGCATGGACGGGCTGAACAACGCCGTCATCCCTGGCTACGCCACGCCCGAGCAGTATGTGAAGGAGATTACCGTCGCC
AATGACGGCATCGTCCACCAGAAGGCGGTGATGGCCTACGCCACCGGCTCCTACCCGATGATCCAGTATCTGGATTCCAT
CGGCGTGAAGTTCGAGAAGGACGGCTCCGGCGAATACAATGTCCGCAAGGTCCATCACATGGGCACCTATGTGCTGCCCA
TGCCGGAGGGCCACCACGTCAAGAAGGCGCTCTATCGCCAGCTGCGCAAGCATCAGGTGCTGGTCACCAACCGCTACATG
GCCACCCGCCTGCTGAACGCCCCGGACGGCCGCATCGCCGGCGCCATGGGGGTGAACACCCGCACCGGCGAATTCCTGGT
GGTGCGGGCGAAGTCCGTCATCCTCGCCTGCGGCGCCGCCGGGCGTCTCGGCCTGCCGGCGTCGGGCTATCTGTTCGGCA
CCTATGAGAACCCGGCCAATTGCGGCGACGGCTACGCCATGGCCTATCATGCCGGCGCGGGCCTCGCGAACCTCGAATGC
TACCAGATCAACCCCTTGATCAAGGATTACAACGGCCCGGCCTGCGCCTATGTCACCGGCCCGTTCGGCGGATACACCGC
CAACAACAAGGGTGAGCGCTTCATCGAGTGCGATTACTGGTCCGGCCAGATGATGAAGGAGTTCTATTCAGAACTTCAGA
GCGGCAACGGCCCGGTCTTCCTCAACATGACCCATCTCGCCGAGGAGACCATCTCCGAGATCGAGACCATCCTGCACTCC
AACGAACGCCCCTCGCGCGGGCGCTTCCACGAGAATCGCGGCACCAACTACCGTGAGAAGATGGTGGAGATGCACATCTC
CGAGATCGGCTTCTGCTCCGGCCATTCGGCTTCGGGCGTGTGGGTGGACGAGAACGCCCGCACCACGGTCGCCGGCCTCT
ATGCCGCCGGCGACATGGCCAGCGTGCCGCACAATTACATGCTCGGCGCCTTCGTGAACGGCGGCATCGCCGGCCAGGAC
TCGGCCGATTACTGCGCCTCCACCGACCTGCCGGCCTATGATGAAGCCGATGTGGTCGCCGAGCAGGAGCGCGTGCTTGC
CCCCACCCGCCGCGACGATGGCCTGACCCCCCACGAGATGGAGTTCAAGACCCGCCGCCTGGTGAATGATTATCTCCAGC
CGCCGAAGGTCACCGCCAAGTATCGCATCGCCCAGGCGCGCTTCGCCGAGATCCGCGAGGACCTCAATGCCCTGTGCGTG
AAGGATCCCCATGAATTGATGCGGGCGCTGGAGCTGCAATCCATCCTCGATTGCGCCGACATGGCCGCTGCCGCCTCGCT
CTATCGCACCGAAAGCCGCTGGGGCCTCTACCACTGGCGGGTGGACCACCCGGCCACCGACAACGAGAACTGGTTCTGCC
ACACGCTGCTCTACAAGGACGAGTTCGGCCGCATGGCCCACAGGAAGCGCGCGGTGGACCCTTACGTGGTCGACATCCCC
GAGGAGGAGATGGGCGCCTACCACCAGCTGCGCACCCCGGCGCTCGCCGCCGAGTGA

Upstream 100 bases:

>100_bases
TGTCCTTCTCCGATGGCCATGCGCGCGGGGTCTACCCCTTCGCCTATCTCGCCGCCCTCGCGGCGGATGCGGCCGGCCCC
GACCTTCCAGAGGTGAAACC

Downstream 100 bases:

>100_bases
CCTCCCAGAATTCGAGACTCCGGAGCCTGCCCCATGCCGTTCGCCAACCATCCCACCTCCGTGCCGGTCACGGTCGACGA
CGAGAAGTGCATTGCCGAGA

Product: putative oxidoreductase

Products: FADH2; Fumarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSEIKTEIVETDILVIGGGTGGPMAAIKAKEKNPGLKVVLMEKANVKRSGAVSMGMDGLNNAVIPGYATPEQYVKEITVA
NDGIVHQKAVMAYATGSYPMIQYLDSIGVKFEKDGSGEYNVRKVHHMGTYVLPMPEGHHVKKALYRQLRKHQVLVTNRYM
ATRLLNAPDGRIAGAMGVNTRTGEFLVVRAKSVILACGAAGRLGLPASGYLFGTYENPANCGDGYAMAYHAGAGLANLEC
YQINPLIKDYNGPACAYVTGPFGGYTANNKGERFIECDYWSGQMMKEFYSELQSGNGPVFLNMTHLAEETISEIETILHS
NERPSRGRFHENRGTNYREKMVEMHISEIGFCSGHSASGVWVDENARTTVAGLYAAGDMASVPHNYMLGAFVNGGIAGQD
SADYCASTDLPAYDEADVVAEQERVLAPTRRDDGLTPHEMEFKTRRLVNDYLQPPKVTAKYRIAQARFAEIREDLNALCV
KDPHELMRALELQSILDCADMAAAASLYRTESRWGLYHWRVDHPATDNENWFCHTLLYKDEFGRMAHRKRAVDPYVVDIP
EEEMGAYHQLRTPALAAE

Sequences:

>Translated_578_residues
MSEIKTEIVETDILVIGGGTGGPMAAIKAKEKNPGLKVVLMEKANVKRSGAVSMGMDGLNNAVIPGYATPEQYVKEITVA
NDGIVHQKAVMAYATGSYPMIQYLDSIGVKFEKDGSGEYNVRKVHHMGTYVLPMPEGHHVKKALYRQLRKHQVLVTNRYM
ATRLLNAPDGRIAGAMGVNTRTGEFLVVRAKSVILACGAAGRLGLPASGYLFGTYENPANCGDGYAMAYHAGAGLANLEC
YQINPLIKDYNGPACAYVTGPFGGYTANNKGERFIECDYWSGQMMKEFYSELQSGNGPVFLNMTHLAEETISEIETILHS
NERPSRGRFHENRGTNYREKMVEMHISEIGFCSGHSASGVWVDENARTTVAGLYAAGDMASVPHNYMLGAFVNGGIAGQD
SADYCASTDLPAYDEADVVAEQERVLAPTRRDDGLTPHEMEFKTRRLVNDYLQPPKVTAKYRIAQARFAEIREDLNALCV
KDPHELMRALELQSILDCADMAAAASLYRTESRWGLYHWRVDHPATDNENWFCHTLLYKDEFGRMAHRKRAVDPYVVDIP
EEEMGAYHQLRTPALAAE
>Mature_577_residues
SEIKTEIVETDILVIGGGTGGPMAAIKAKEKNPGLKVVLMEKANVKRSGAVSMGMDGLNNAVIPGYATPEQYVKEITVAN
DGIVHQKAVMAYATGSYPMIQYLDSIGVKFEKDGSGEYNVRKVHHMGTYVLPMPEGHHVKKALYRQLRKHQVLVTNRYMA
TRLLNAPDGRIAGAMGVNTRTGEFLVVRAKSVILACGAAGRLGLPASGYLFGTYENPANCGDGYAMAYHAGAGLANLECY
QINPLIKDYNGPACAYVTGPFGGYTANNKGERFIECDYWSGQMMKEFYSELQSGNGPVFLNMTHLAEETISEIETILHSN
ERPSRGRFHENRGTNYREKMVEMHISEIGFCSGHSASGVWVDENARTTVAGLYAAGDMASVPHNYMLGAFVNGGIAGQDS
ADYCASTDLPAYDEADVVAEQERVLAPTRRDDGLTPHEMEFKTRRLVNDYLQPPKVTAKYRIAQARFAEIREDLNALCVK
DPHELMRALELQSILDCADMAAAASLYRTESRWGLYHWRVDHPATDNENWFCHTLLYKDEFGRMAHRKRAVDPYVVDIPE
EEMGAYHQLRTPALAAE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=453, Percent_Identity=26.7108167770419, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790597, Length=557, Percent_Identity=22.9802513464991, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 63837; Mature: 63706

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKTEIVETDILVIGGGTGGPMAAIKAKEKNPGLKVVLMEKANVKRSGAVSMGMDGLN
CCCHHHHHEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCEECCCCCC
NAVIPGYATPEQYVKEITVANDGIVHQKAVMAYATGSYPMIQYLDSIGVKFEKDGSGEYN
CCCCCCCCCHHHHHHHHHCCCCCCEEHHEEEEEECCCCHHHHHHHHCCCEEECCCCCCCE
VRKVHHMGTYVLPMPEGHHVKKALYRQLRKHQVLVTNRYMATRLLNAPDGRIAGAMGVNT
EEEEEECCEEEEECCCCCHHHHHHHHHHHHCEEEEECCHHHHHHCCCCCCCEEEEECCCC
RTGEFLVVRAKSVILACGAAGRLGLPASGYLFGTYENPANCGDGYAMAYHAGAGLANLEC
CCCCEEEEEECEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEE
YQINPLIKDYNGPACAYVTGPFGGYTANNKGERFIECDYWSGQMMKEFYSELQSGNGPVF
EEECCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEE
LNMTHLAEETISEIETILHSNERPSRGRFHENRGTNYREKMVEMHISEIGFCSGHSASGV
EEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCE
WVDENARTTVAGLYAAGDMASVPHNYMLGAFVNGGIAGQDSADYCASTDLPAYDEADVVA
EECCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHH
EQERVLAPTRRDDGLTPHEMEFKTRRLVNDYLQPPKVTAKYRIAQARFAEIREDLNALCV
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEC
KDPHELMRALELQSILDCADMAAAASLYRTESRWGLYHWRVDHPATDNENWFCHTLLYKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEEHH
EFGRMAHRKRAVDPYVVDIPEEEMGAYHQLRTPALAAE
HHHHHHHHHHCCCCEEEECCHHHHCHHHHHCCCCCCCC
>Mature Secondary Structure 
SEIKTEIVETDILVIGGGTGGPMAAIKAKEKNPGLKVVLMEKANVKRSGAVSMGMDGLN
CCHHHHHEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCEECCCCCC
NAVIPGYATPEQYVKEITVANDGIVHQKAVMAYATGSYPMIQYLDSIGVKFEKDGSGEYN
CCCCCCCCCHHHHHHHHHCCCCCCEEHHEEEEEECCCCHHHHHHHHCCCEEECCCCCCCE
VRKVHHMGTYVLPMPEGHHVKKALYRQLRKHQVLVTNRYMATRLLNAPDGRIAGAMGVNT
EEEEEECCEEEEECCCCCHHHHHHHHHHHHCEEEEECCHHHHHHCCCCCCCEEEEECCCC
RTGEFLVVRAKSVILACGAAGRLGLPASGYLFGTYENPANCGDGYAMAYHAGAGLANLEC
CCCCEEEEEECEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCEEE
YQINPLIKDYNGPACAYVTGPFGGYTANNKGERFIECDYWSGQMMKEFYSELQSGNGPVF
EEECCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEE
LNMTHLAEETISEIETILHSNERPSRGRFHENRGTNYREKMVEMHISEIGFCSGHSASGV
EEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCE
WVDENARTTVAGLYAAGDMASVPHNYMLGAFVNGGIAGQDSADYCASTDLPAYDEADVVA
EECCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHH
EQERVLAPTRRDDGLTPHEMEFKTRRLVNDYLQPPKVTAKYRIAQARFAEIREDLNALCV
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEC
KDPHELMRALELQSILDCADMAAAASLYRTESRWGLYHWRVDHPATDNENWFCHTLLYKD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEEHH
EFGRMAHRKRAVDPYVVDIPEEEMGAYHQLRTPALAAE
HHHHHHHHHHCCCCEEEECCHHHHCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]