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The map label for this gene is greA

Identifier: 154246690

GI number: 154246690

Start: 3047774

End: 3048280

Strand: Direct

Name: greA

Synonym: Xaut_2751

Alternate gene names: 154246690

Gene position: 3047774-3048280 (Clockwise)

Preceding gene: 154246689

Following gene: 154246692

Centisome position: 57.41

GC content: 66.07

Gene sequence:

>507_bases
GTGACCTGTTTCAGGAGGTCTGAAGAAGGACCCATGGAGAAGATTCCCATGACGGCGGCGGGCTACGCCGCCCTCGCGCA
AGAGTTGAAGCAGCGCCAGCAGGTGGAGCGTCCGCGCATCATCAGCGCCATCTCCGAGGCCCGCGCCCATGGCGACCTCT
CCGAGAATGCGGAATATCACGCTGCCAAGGAGCAGCAGTCGCTGAATGAGGGGCGGGTCGCCGATCTCGAGGACAAGCTG
TCCCGGGCCGAGGTGATCGACGTGTCCAAGCTGTCGGGCGACATCGTGAAGTTCGGCGCCACCGTGACCCTCGTGGACGA
GGACACGGAGGAGGAGAAGGTCTGGCAGATCGTCGGCGACAGCGAAGCCGACGCCAAGGCGGGTCGCATCTCCATCTCCT
CGCCCGTGGCTCGCGCGCTCATCGGCAAGAAGAAGGGCGCCTCGGTGGAGGTGGTCACGCCCAAGGGCGCGCGCTCCTTC
GAGATCGTGGCGGTGCGCTGGGCCTGA

Upstream 100 bases:

>100_bases
CCGCCGGGCGGGGGCAGCCGCCGCAACCGTGGGCCAACGCCGCACGGTGCGGCTTTCTTTTCTAGCGCCGGAGGCGCCCC
CTCCAGGGGTGTCCGGCGCA

Downstream 100 bases:

>100_bases
GCCGTATTCTCCTCACACCCTCTCCCGCACGCGGGGGAGGGGCTGCGGGCGGCTTCAGGACACGTCGTCTCACGCCCCCC
GGATCTCGCCCCCCTGGATC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTCFRRSEEGPMEKIPMTAAGYAALAQELKQRQQVERPRIISAISEARAHGDLSENAEYHAAKEQQSLNEGRVADLEDKL
SRAEVIDVSKLSGDIVKFGATVTLVDEDTEEEKVWQIVGDSEADAKAGRISISSPVARALIGKKKGASVEVVTPKGARSF
EIVAVRWA

Sequences:

>Translated_168_residues
MTCFRRSEEGPMEKIPMTAAGYAALAQELKQRQQVERPRIISAISEARAHGDLSENAEYHAAKEQQSLNEGRVADLEDKL
SRAEVIDVSKLSGDIVKFGATVTLVDEDTEEEKVWQIVGDSEADAKAGRISISSPVARALIGKKKGASVEVVTPKGARSF
EIVAVRWA
>Mature_167_residues
TCFRRSEEGPMEKIPMTAAGYAALAQELKQRQQVERPRIISAISEARAHGDLSENAEYHAAKEQQSLNEGRVADLEDKLS
RAEVIDVSKLSGDIVKFGATVTLVDEDTEEEKVWQIVGDSEADAKAGRISISSPVARALIGKKKGASVEVVTPKGARSFE
IVAVRWA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=52.8662420382166, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18280; Mature: 18149

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCFRRSEEGPMEKIPMTAAGYAALAQELKQRQQVERPRIISAISEARAHGDLSENAEYH
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHH
AAKEQQSLNEGRVADLEDKLSRAEVIDVSKLSGDIVKFGATVTLVDEDTEEEKVWQIVGD
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCHHHHHHEECC
SEADAKAGRISISSPVARALIGKKKGASVEVVTPKGARSFEIVAVRWA
CCCCCCCCEEECCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure 
TCFRRSEEGPMEKIPMTAAGYAALAQELKQRQQVERPRIISAISEARAHGDLSENAEYH
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHH
AAKEQQSLNEGRVADLEDKLSRAEVIDVSKLSGDIVKFGATVTLVDEDTEEEKVWQIVGD
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCHHHHHHEECC
SEADAKAGRISISSPVARALIGKKKGASVEVVTPKGARSFEIVAVRWA
CCCCCCCCEEECCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA