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The map label for this gene is nudF [H]

Identifier: 154246578

GI number: 154246578

Start: 2928116

End: 2928769

Strand: Direct

Name: nudF [H]

Synonym: Xaut_2637

Alternate gene names: 154246578

Gene position: 2928116-2928769 (Clockwise)

Preceding gene: 154246577

Following gene: 154246580

Centisome position: 55.15

GC content: 71.41

Gene sequence:

>654_bases
ATGACGGGGCAAGAGCGGACCCTCATGGAGGACCGCCCCGCGCGGGTGGACGTGGAGGGGCCGGAGCTGCTGGCCAAGGG
CTTCCGGCCCTATGAGCGCTACCGCCTGGTGCTGCACCATGACGACGGCACCTCCGACGCCCAGGTGCGCGACATCGTGC
GCGGCGGCCGCGTGGTGGGGGTGCTCGGCTATGATCCCGGGCGCGACATGGTGGTCCTGATCCGCCAGTTCCGCCTCACC
GCCCATCTCCTCCCCCAGGGGCGGGGGGAGATGGTGGAGATCGTGGCCGGGCTGGTGGAAGCGGGCGAGGACCCGGAAGC
CGCAGCCGTGCGCGAATGCATCGAAGAGACGGGCGTTGAGCCGCGCCTCGTCCTGCCCATGCTCACGTTCACGCCGACGC
CGGGCGTCACCGACGAATTCGCCCTGATGTACCTCGGCATCCTCGATGCCGGGGCGCTCCCCGCGCGGGCCGGGGCGCGG
GACGAGACGGAGATGACGCAGCCGTTCGCGGTGCCGGTGGACGAGGCGCTCGCTGCCATCGCGCAGGGGCGCTGCATCAA
TGGCTTCCTCATCCTGGCCCTGCAATGGCTGGCGCTCAACCGCGCCGGCCTGCCGGACCTGATCGCGGCGGCGGAGCACG
AGGAGCAATCTTGA

Upstream 100 bases:

>100_bases
AGGCGCGCGCCGTGTTCGAGCAGCCGAAGGAGGCCTATACGCGGGCCCTGTTCGCCGCCGCCTTCAATCTGGAGATCGCC
GGCCAGGGCGTGGTCCGCCA

Downstream 100 bases:

>100_bases
GCCCACGCGCCGCCTGAGCGAAACCCTTCCGGTATCACACGTGGTGGTCGAAGCGCTCCAGGTCGAAGTTCGCCACGTCC
CTTAGCAGTTCGATGAAGGC

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTGQERTLMEDRPARVDVEGPELLAKGFRPYERYRLVLHHDDGTSDAQVRDIVRGGRVVGVLGYDPGRDMVVLIRQFRLT
AHLLPQGRGEMVEIVAGLVEAGEDPEAAAVRECIEETGVEPRLVLPMLTFTPTPGVTDEFALMYLGILDAGALPARAGAR
DETEMTQPFAVPVDEALAAIAQGRCINGFLILALQWLALNRAGLPDLIAAAEHEEQS

Sequences:

>Translated_217_residues
MTGQERTLMEDRPARVDVEGPELLAKGFRPYERYRLVLHHDDGTSDAQVRDIVRGGRVVGVLGYDPGRDMVVLIRQFRLT
AHLLPQGRGEMVEIVAGLVEAGEDPEAAAVRECIEETGVEPRLVLPMLTFTPTPGVTDEFALMYLGILDAGALPARAGAR
DETEMTQPFAVPVDEALAAIAQGRCINGFLILALQWLALNRAGLPDLIAAAEHEEQS
>Mature_216_residues
TGQERTLMEDRPARVDVEGPELLAKGFRPYERYRLVLHHDDGTSDAQVRDIVRGGRVVGVLGYDPGRDMVVLIRQFRLTA
HLLPQGRGEMVEIVAGLVEAGEDPEAAAVRECIEETGVEPRLVLPMLTFTPTPGVTDEFALMYLGILDAGALPARAGARD
ETEMTQPFAVPVDEALAAIAQGRCINGFLILALQWLALNRAGLPDLIAAAEHEEQS

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=192, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1788810, Length=194, Percent_Identity=27.319587628866, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23621; Mature: 23490

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQERTLMEDRPARVDVEGPELLAKGFRPYERYRLVLHHDDGTSDAQVRDIVRGGRVVG
CCCCCCCHHCCCCCEECCCCHHHHHHCCCCHHHEEEEEEECCCCCHHHHHHHHHCCCEEE
VLGYDPGRDMVVLIRQFRLTAHLLPQGRGEMVEIVAGLVEAGEDPEAAAVRECIEETGVE
EECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
PRLVLPMLTFTPTPGVTDEFALMYLGILDAGALPARAGARDETEMTQPFAVPVDEALAAI
CEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AQGRCINGFLILALQWLALNRAGLPDLIAAAEHEEQS
HCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TGQERTLMEDRPARVDVEGPELLAKGFRPYERYRLVLHHDDGTSDAQVRDIVRGGRVVG
CCCCCCHHCCCCCEECCCCHHHHHHCCCCHHHEEEEEEECCCCCHHHHHHHHHCCCEEE
VLGYDPGRDMVVLIRQFRLTAHLLPQGRGEMVEIVAGLVEAGEDPEAAAVRECIEETGVE
EECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
PRLVLPMLTFTPTPGVTDEFALMYLGILDAGALPARAGARDETEMTQPFAVPVDEALAAI
CEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AQGRCINGFLILALQWLALNRAGLPDLIAAAEHEEQS
HCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]