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The map label for this gene is rph

Identifier: 154246476

GI number: 154246476

Start: 2832104

End: 2832817

Strand: Direct

Name: rph

Synonym: Xaut_2535

Alternate gene names: 154246476

Gene position: 2832104-2832817 (Clockwise)

Preceding gene: 154246473

Following gene: 154246477

Centisome position: 53.35

GC content: 67.65

Gene sequence:

>714_bases
ATGCGCCCCAGCAAGCGCGCCGCCGACGAGATGCGCGCGGTCTCCTTTGAACGGGGCGTGATGCGCCACGCGGAAGGCTC
CTGCCTCGTGAAGTTCGGCGACACCCATGTGCTCGTCTCCGCCACGCTGGAAGAGCGCCTGCCGCCGTGGCTGAAGGGCC
AGGGCCGGGGCTGGGTGACGGCGGAATATTCCATGCTGCCCCGCGCCACCCACGATCGCACGCGGCGTGAATCCACCACC
GGCAAGCAGTCCGGCCGCACCCAGGAAATCCAGCGCCTGATCGGCCGGTCGCTGCGCTCGGTCACCGATCTCGTGGCCCT
GGGCGAGAAGCAGATAACCCTCGACTGCGACGTGCTGCAGGCCGATGGCGGCACCCGCACCGCCGCCATCACCGGCGCCT
GGATCGCGCTTTACGACTGCCTGAGCTGGATGCGTCAGCGCTCCATGGTCAAGGAGATCCCGCTCAAGGACCACGTGGCG
GCGGTGTCCTGCGGCATCTACAACGGCACGCCGGTGCTCGACCTTGACTATGCCGAGGATTCGGTGGCCGAGACTGACGC
CAATTTCGTCATGACCGGCACCGGCGGCATCGTCGAGATCCAGGGCACGGCGGAAAAGACCCCGTTCTCGCAGGACGAAC
TGCTGGGCCTTTTGTCCCTCGCCCGTTCCGGCGTGGAGAAGCTGGTGGGGCTGCAGAAGCTGGCGGTGGGCTGA

Upstream 100 bases:

>100_bases
TCTTCGGCGTCTGCCGCATTTCCTTCACGCGAACCGGTCTCCACTTCGCTCGAAAATGCTCTAAAAGGGGCGCCCGATGA
CCAGACAAGAGGTAAGTCCC

Downstream 100 bases:

>100_bases
TGGACCATCGCATCCTCAAGGGCCGGCTCGTGGTGGCCACCCACAATCCCGGCAAGCTCATCGAGATGCGCATGCTGCTC
GCCCCCCACGGGGTGGAGGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSKRAADEMRAVSFERGVMRHAEGSCLVKFGDTHVLVSATLEERLPPWLKGQGRGWVTAEYSMLPRATHDRTRRESTT
GKQSGRTQEIQRLIGRSLRSVTDLVALGEKQITLDCDVLQADGGTRTAAITGAWIALYDCLSWMRQRSMVKEIPLKDHVA
AVSCGIYNGTPVLDLDYAEDSVAETDANFVMTGTGGIVEIQGTAEKTPFSQDELLGLLSLARSGVEKLVGLQKLAVG

Sequences:

>Translated_237_residues
MRPSKRAADEMRAVSFERGVMRHAEGSCLVKFGDTHVLVSATLEERLPPWLKGQGRGWVTAEYSMLPRATHDRTRRESTT
GKQSGRTQEIQRLIGRSLRSVTDLVALGEKQITLDCDVLQADGGTRTAAITGAWIALYDCLSWMRQRSMVKEIPLKDHVA
AVSCGIYNGTPVLDLDYAEDSVAETDANFVMTGTGGIVEIQGTAEKTPFSQDELLGLLSLARSGVEKLVGLQKLAVG
>Mature_237_residues
MRPSKRAADEMRAVSFERGVMRHAEGSCLVKFGDTHVLVSATLEERLPPWLKGQGRGWVTAEYSMLPRATHDRTRRESTT
GKQSGRTQEIQRLIGRSLRSVTDLVALGEKQITLDCDVLQADGGTRTAAITGAWIALYDCLSWMRQRSMVKEIPLKDHVA
AVSCGIYNGTPVLDLDYAEDSVAETDANFVMTGTGGIVEIQGTAEKTPFSQDELLGLLSLARSGVEKLVGLQKLAVG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=60.1895734597156, Blast_Score=261, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI71981632, Length=181, Percent_Identity=26.5193370165746, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_XANP2 (A7IID4)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001417434.1
- ProteinModelPortal:   A7IID4
- SMR:   A7IID4
- STRING:   A7IID4
- GeneID:   5424519
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_2535
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   XAUT78245:XAUT_2535-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25850; Mature: 25850

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSKRAADEMRAVSFERGVMRHAEGSCLVKFGDTHVLVSATLEERLPPWLKGQGRGWVT
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEHHHHCCCCCCCCCCEEEE
AEYSMLPRATHDRTRRESTTGKQSGRTQEIQRLIGRSLRSVTDLVALGEKQITLDCDVLQ
EEHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTAAITGAWIALYDCLSWMRQRSMVKEIPLKDHVAAVSCGIYNGTPVLDLDYAED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEECCCCC
SVAETDANFVMTGTGGIVEIQGTAEKTPFSQDELLGLLSLARSGVEKLVGLQKLAVG
HHHHCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSKRAADEMRAVSFERGVMRHAEGSCLVKFGDTHVLVSATLEERLPPWLKGQGRGWVT
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEHHHHCCCCCCCCCCEEEE
AEYSMLPRATHDRTRRESTTGKQSGRTQEIQRLIGRSLRSVTDLVALGEKQITLDCDVLQ
EEHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTAAITGAWIALYDCLSWMRQRSMVKEIPLKDHVAAVSCGIYNGTPVLDLDYAED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEECCCCC
SVAETDANFVMTGTGGIVEIQGTAEKTPFSQDELLGLLSLARSGVEKLVGLQKLAVG
HHHHCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA