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The map label for this gene is mcl2 [H]

Identifier: 154245917

GI number: 154245917

Start: 2230130

End: 2231032

Strand: Reverse

Name: mcl2 [H]

Synonym: Xaut_1974

Alternate gene names: 154245917

Gene position: 2231032-2230130 (Counterclockwise)

Preceding gene: 154245918

Following gene: 154245916

Centisome position: 42.02

GC content: 71.43

Gene sequence:

>903_bases
ATGGGTGCCGAAGCTGGAGCCGCCATTGCCATCCGCCCGCGCCGCAGCGTGCTCTACATGCCGGGCTCCAATGCCCGCGC
GCTGGAAAAGGCCCGCACCCTGCCCGCCGACGCGGTGATCCTGGACCTTGAGGACGCGGTGGCCCCCGATGCCAAGGCGG
AAGCCCGCGCCCGCATCGTGGATACGGTGAAGGCCGGCGGCTTCGGCCCCCGCGAGGTGGTCATCCGCATCAACGGCCTC
GACACGCCGTGGGGCTCTGACGACCTGGAGGCCGCCGCCGGCGCTGCGCCCGACGCCATCCTGGTGCCCAAGGTGCAGAC
CGTGGAGCAGCTGGTCACGGTGGGCCGCCGGCTGGAGGCGCTGAAGGTGCCGCAGACCACGCGGGTCTGGGCCATGATCG
AGACCCCGCTCGCCATCCTCGACATCAAGGCCATCGCCCAGGCGGCGCAGGACCCGCTGACCCGGCTCACGGTGTTCGTG
CTCGGCACCAACGACCTCGCCAAGGAGACGCGGGCGGCGCTGGTGCCCGGCCGCGCGCCCATGGTGGGCTGGCTGAGCCT
GGTGGTGGCCGCCGCGCGGGTCTATGGCGTGGACGTGCTCGACGGGGTGTGGAACCAGTTCCAGGACCTCGACGGCTTCA
AGGCCGAATCCGCGCAGGCGGTGGAATTCGGCATGGACGGCAAGACCCTGATCCATCCGAGCCAGATCGAGCCCTGCAAC
ATCGCCTTTTCGCCGCCCGAGGCGGAAGTGGCCCGCGCCCGCGCCATCATCGCCGCCTTCGCGCTGCCGGAGAATGCGGG
CAAGGGCGTCATCACCCTTGACGGGCGCATGGTGGAACTGCTCCACGCCGAGATGGCCAAGCGCACGGTGGCCCTCGCCG
ACGCCATCGCCGCCCGCGCCTGA

Upstream 100 bases:

>100_bases
GTCGCATGCCGCAGTGCAGCGGCACCCCTTTTCAAAGCACGGGCGTCGCTTTACCGATTCGATGCGATAAGATCCGCATC
GACGTGCGAAGGGAGTCCCC

Downstream 100 bases:

>100_bases
GGTTCTAGCCCGCATGTCCCAGCCCGCCGAGGCCCCGTTCCGCATTGTCATGCTCGCCTACCCGCGCATGACCCAGCTCG
ATCTCACCGGCCCGTTCGAG

Product: Citryl-CoA lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MGAEAGAAIAIRPRRSVLYMPGSNARALEKARTLPADAVILDLEDAVAPDAKAEARARIVDTVKAGGFGPREVVIRINGL
DTPWGSDDLEAAAGAAPDAILVPKVQTVEQLVTVGRRLEALKVPQTTRVWAMIETPLAILDIKAIAQAAQDPLTRLTVFV
LGTNDLAKETRAALVPGRAPMVGWLSLVVAAARVYGVDVLDGVWNQFQDLDGFKAESAQAVEFGMDGKTLIHPSQIEPCN
IAFSPPEAEVARARAIIAAFALPENAGKGVITLDGRMVELLHAEMAKRTVALADAIAARA

Sequences:

>Translated_300_residues
MGAEAGAAIAIRPRRSVLYMPGSNARALEKARTLPADAVILDLEDAVAPDAKAEARARIVDTVKAGGFGPREVVIRINGL
DTPWGSDDLEAAAGAAPDAILVPKVQTVEQLVTVGRRLEALKVPQTTRVWAMIETPLAILDIKAIAQAAQDPLTRLTVFV
LGTNDLAKETRAALVPGRAPMVGWLSLVVAAARVYGVDVLDGVWNQFQDLDGFKAESAQAVEFGMDGKTLIHPSQIEPCN
IAFSPPEAEVARARAIIAAFALPENAGKGVITLDGRMVELLHAEMAKRTVALADAIAARA
>Mature_299_residues
GAEAGAAIAIRPRRSVLYMPGSNARALEKARTLPADAVILDLEDAVAPDAKAEARARIVDTVKAGGFGPREVVIRINGLD
TPWGSDDLEAAAGAAPDAILVPKVQTVEQLVTVGRRLEALKVPQTTRVWAMIETPLAILDIKAIAQAAQDPLTRLTVFVL
GTNDLAKETRAALVPGRAPMVGWLSLVVAAARVYGVDVLDGVWNQFQDLDGFKAESAQAVEFGMDGKTLIHPSQIEPCNI
AFSPPEAEVARARAIIAAFALPENAGKGVITLDGRMVELLHAEMAKRTVALADAIAARA

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=309, Percent_Identity=28.8025889967638, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI87081764, Length=275, Percent_Identity=32.3636363636364, Blast_Score=143, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17557458, Length=282, Percent_Identity=28.3687943262411, Blast_Score=120, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 31658; Mature: 31527

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAEAGAAIAIRPRRSVLYMPGSNARALEKARTLPADAVILDLEDAVAPDAKAEARARIV
CCCCCCCEEEEECCCEEEEECCCCHHHHHHHHCCCCCEEEEECHHHCCCCCHHHHHHHHH
DTVKAGGFGPREVVIRINGLDTPWGSDDLEAAAGAAPDAILVPKVQTVEQLVTVGRRLEA
HHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHH
LKVPQTTRVWAMIETPLAILDIKAIAQAAQDPLTRLTVFVLGTNDLAKETRAALVPGRAP
HCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCCCH
MVGWLSLVVAAARVYGVDVLDGVWNQFQDLDGFKAESAQAVEFGMDGKTLIHPSQIEPCN
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCCCCCCCE
IAFSPPEAEVARARAIIAAFALPENAGKGVITLDGRMVELLHAEMAKRTVALADAIAARA
EEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GAEAGAAIAIRPRRSVLYMPGSNARALEKARTLPADAVILDLEDAVAPDAKAEARARIV
CCCCCCEEEEECCCEEEEECCCCHHHHHHHHCCCCCEEEEECHHHCCCCCHHHHHHHHH
DTVKAGGFGPREVVIRINGLDTPWGSDDLEAAAGAAPDAILVPKVQTVEQLVTVGRRLEA
HHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHH
LKVPQTTRVWAMIETPLAILDIKAIAQAAQDPLTRLTVFVLGTNDLAKETRAALVPGRAP
HCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCCCH
MVGWLSLVVAAARVYGVDVLDGVWNQFQDLDGFKAESAQAVEFGMDGKTLIHPSQIEPCN
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEECCCCCCCCE
IAFSPPEAEVARARAIIAAFALPENAGKGVITLDGRMVELLHAEMAKRTVALADAIAARA
EEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA