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The map label for this gene is menB [C]

Identifier: 154245800

GI number: 154245800

Start: 2089362

End: 2090153

Strand: Reverse

Name: menB [C]

Synonym: Xaut_1856

Alternate gene names: 154245800

Gene position: 2090153-2089362 (Counterclockwise)

Preceding gene: 154245801

Following gene: 154245799

Centisome position: 39.37

GC content: 69.07

Gene sequence:

>792_bases
ATGAGCCTCAACACCACCGGCCTCATCACCACCGTCGAGGATGGCGTCGCCGTCATCACCCTCGACCGTCCGGAGGTGCG
CAACGCCTTCGACGATGCGCTCATCGCCGCGCTGACCCGCGCCTATGATGCCGCCGCCGTCGATCCACAGGTCTACGCCG
TCCTGCTGAAGGCCAACGGCCCCACCTTTTGTGCCGGGGCGGACCTCAACTGGATGCGTCGCATGGCGACCTATAGCCGG
CAGGAGAACCTCGCCGACGCCATGGCGCTGGCGACGCTCATGCGCACCATCGACCTGTGCCCCAAGCCCACCCTGGTGCG
AGTGCACGGCTCGGCCTTTGCCGGCGCCACCGGGCTGATCGCGGCCAGCGACATCGTCGTCGCGGTGCCGGAGGCGGAAT
TCGCGATCACCGAGGTGCGCATCGGCCTCATCCCCTCGGTCATCAGCCCCTATCTGGTGCGGGCCATGGGGGCGCGGCAG
GCGCGGCGCTATTTCCTCACCGCCGAGCGCTTTTCCGCCGAAACGGCGCTCGCCCTCGGCCTCGTGCATGAGGTGGTGAG
CGCAGAGGAACTGGACGCCGCCATCGTCCGGCACCTCAAGGCGCTGAAGGCCGCGAGCCCCGGCGCCATGGCCGCCACAA
AGGCTCTGATCGCCGCCGTGGACCGGCCCTTCGACCAGACCGTGCTGCAGGACACCGCCCGCCGCATCGCCGACCAGCGC
GTGAGCGCCGACGGACGCGAGGGCGTTTCCGCCTTCCTCGAAAAAAGAAAACCCACTTGGGGGGCCGCATGA

Upstream 100 bases:

>100_bases
GCCCTCTCCGCCGCGCTCAACGCCCCCGAGCAGGAGACGCGCTTCGGCGTGTTCCGCATGTGATGGCGCGCGTTGACGGC
CAAGTCCGGAGCACCATCCC

Downstream 100 bases:

>100_bases
CCAAAGCGATCCGCACCCTGCTCGTCGCCAATCGCGGCGAGATCGCCGTGCGCGTGATGCGCACCGCCAAGGCCATGGGC
ATCCGCACCGTCGCCGTCTA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSLNTTGLITTVEDGVAVITLDRPEVRNAFDDALIAALTRAYDAAAVDPQVYAVLLKANGPTFCAGADLNWMRRMATYSR
QENLADAMALATLMRTIDLCPKPTLVRVHGSAFAGATGLIAASDIVVAVPEAEFAITEVRIGLIPSVISPYLVRAMGARQ
ARRYFLTAERFSAETALALGLVHEVVSAEELDAAIVRHLKALKAASPGAMAATKALIAAVDRPFDQTVLQDTARRIADQR
VSADGREGVSAFLEKRKPTWGAA

Sequences:

>Translated_263_residues
MSLNTTGLITTVEDGVAVITLDRPEVRNAFDDALIAALTRAYDAAAVDPQVYAVLLKANGPTFCAGADLNWMRRMATYSR
QENLADAMALATLMRTIDLCPKPTLVRVHGSAFAGATGLIAASDIVVAVPEAEFAITEVRIGLIPSVISPYLVRAMGARQ
ARRYFLTAERFSAETALALGLVHEVVSAEELDAAIVRHLKALKAASPGAMAATKALIAAVDRPFDQTVLQDTARRIADQR
VSADGREGVSAFLEKRKPTWGAA
>Mature_262_residues
SLNTTGLITTVEDGVAVITLDRPEVRNAFDDALIAALTRAYDAAAVDPQVYAVLLKANGPTFCAGADLNWMRRMATYSRQ
ENLADAMALATLMRTIDLCPKPTLVRVHGSAFAGATGLIAASDIVVAVPEAEFAITEVRIGLIPSVISPYLVRAMGARQA
RRYFLTAERFSAETALALGLVHEVVSAEELDAAIVRHLKALKAASPGAMAATKALIAAVDRPFDQTVLQDTARRIADQRV
SADGREGVSAFLEKRKPTWGAA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=30.46875, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=30.6772908366534, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI45643119, Length=254, Percent_Identity=26.3779527559055, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260275230, Length=254, Percent_Identity=26.3779527559055, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=254, Percent_Identity=26.3779527559055, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI70995211, Length=221, Percent_Identity=28.5067873303167, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594471, Length=205, Percent_Identity=27.3170731707317, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI37594469, Length=205, Percent_Identity=27.3170731707317, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI157694516, Length=210, Percent_Identity=25.2380952380952, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI213417737, Length=210, Percent_Identity=25.2380952380952, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221136756, Length=213, Percent_Identity=25.3521126760563, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136753, Length=213, Percent_Identity=25.3521126760563, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221307494, Length=213, Percent_Identity=25.3521126760563, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=29.718875502008, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=33.969465648855, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=29.5019157088123, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=28.0898876404494, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=29.4573643410853, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=28.3464566929134, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=258, Percent_Identity=25.968992248062, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=29.3172690763052, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=189, Percent_Identity=27.5132275132275, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=234, Percent_Identity=27.3504273504274, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=27.3148148148148, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=183, Percent_Identity=26.2295081967213, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=263, Percent_Identity=25.4752851711027, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=235, Percent_Identity=26.8085106382979, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=198, Percent_Identity=28.7878787878788, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=31.4285714285714, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=31.4285714285714, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922422, Length=248, Percent_Identity=24.5967741935484, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19920382, Length=175, Percent_Identity=29.7142857142857, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28021; Mature: 27889

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNTTGLITTVEDGVAVITLDRPEVRNAFDDALIAALTRAYDAAAVDPQVYAVLLKANG
CCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCC
PTFCAGADLNWMRRMATYSRQENLADAMALATLMRTIDLCPKPTLVRVHGSAFAGATGLI
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHE
AASDIVVAVPEAEFAITEVRIGLIPSVISPYLVRAMGARQARRYFLTAERFSAETALALG
EECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVHEVVSAEELDAAIVRHLKALKAASPGAMAATKALIAAVDRPFDQTVLQDTARRIADQR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VSADGREGVSAFLEKRKPTWGAA
CCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SLNTTGLITTVEDGVAVITLDRPEVRNAFDDALIAALTRAYDAAAVDPQVYAVLLKANG
CCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCC
PTFCAGADLNWMRRMATYSRQENLADAMALATLMRTIDLCPKPTLVRVHGSAFAGATGLI
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHE
AASDIVVAVPEAEFAITEVRIGLIPSVISPYLVRAMGARQARRYFLTAERFSAETALALG
EECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVHEVVSAEELDAAIVRHLKALKAASPGAMAATKALIAAVDRPFDQTVLQDTARRIADQR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VSADGREGVSAFLEKRKPTWGAA
CCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA