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The map label for this gene is mvaB [H]

Identifier: 154245798

GI number: 154245798

Start: 2086460

End: 2087365

Strand: Reverse

Name: mvaB [H]

Synonym: Xaut_1854

Alternate gene names: 154245798

Gene position: 2087365-2086460 (Counterclockwise)

Preceding gene: 154245799

Following gene: 154245797

Centisome position: 39.32

GC content: 70.75

Gene sequence:

>906_bases
ATGGCCCGCCCCCCGTCGGTGCGCATCGTCGAGGTCGGCCCGCGCGACGGGCTGCAGAACGAACCGGGCGTCGTCCCGCT
CGCCGCCAAGGTGGCGCTCATCAATGCGCTGGCCGAAGCCGGGCTGACGATGATCGAGAGCGGCTCCTTCGTCTCCCCCA
AATGGGTGCCGCAGATGGCGGACACCGCGCAGGTGCTGGCTGAGATCGACCGGCGCGACGGCGTGCGCTACCCGGTGCTC
ACTCCCAACCTGAAGGGGCTGGAACTGGCGCTGGCCTCCGGCGTGGAGGAGGTGGCGGTGTTCGCCGCTGCATCCGAGGC
CTTCAGCCAGAAGAACATCAATTGCACCATCGCCGAGAGCCTGGAGCGCTTCGCGCCCGTGCTCGACCTCGCGAAGGCGC
AGGGGGTGGCGGTGCGCGGCTATGTGAGCTGCGTGCTCGGCTGCCCCTATCAGGGCGAGGTGCCGGTGGCCAAGGTGGTG
GAGGTGTCCGAACGCCTCATCGCCATGGGCTGCTATGAGGTTTCGCTGGGCGACACCATCGGTGTCGGCACCCCGGACAA
GGCGAAGGCCATGGCGCAGGCGGTGGCTGGCGTGATCGGCGTGGAGCGCACCGCCCTGCATTTCCACGACACCTACGGGC
AGGCGCTGGCCAATGTGCTGGCCTGCCTGGAGCTGGGCATCGCCACGGTGGATGCGGCGGCGGCCGGCCTCGGCGGCTGC
CCCTATGCCAAGGGCGCCTCTGGGAATCTCGCCACCGAGGATCTCGTCTACATGCTGGACGGCCTCGGCGTGGACACCGG
CGTGGATCTCGACGCCCTCGCGCGCGCCGGCCGCGCCATCACCGCCGCGCTGAGCCGCACGCCGGCCTCCAAGGTCGCAC
AGGCGCTGGCCGCCAAGGCGGGGTAG

Upstream 100 bases:

>100_bases
CCGTCGCCGCGCCCCGCGACGGCAAGGTGAAGACGCTGAAATTCGCCGTGGGCGACCTCGTGGACGAGGGTGCCGAGCTT
CTGGTTCTGGAGGACGCGTG

Downstream 100 bases:

>100_bases
AGGGGGCGTCCGCTCCACCAGAACGCGGCAGTCACTTCAACGAGACGCGCCGCCAACCCGCGCGATCTCCTCCTCCCACG
TCCCTGCCCCCTCCAGAACC

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MARPPSVRIVEVGPRDGLQNEPGVVPLAAKVALINALAEAGLTMIESGSFVSPKWVPQMADTAQVLAEIDRRDGVRYPVL
TPNLKGLELALASGVEEVAVFAAASEAFSQKNINCTIAESLERFAPVLDLAKAQGVAVRGYVSCVLGCPYQGEVPVAKVV
EVSERLIAMGCYEVSLGDTIGVGTPDKAKAMAQAVAGVIGVERTALHFHDTYGQALANVLACLELGIATVDAAAAGLGGC
PYAKGASGNLATEDLVYMLDGLGVDTGVDLDALARAGRAITAALSRTPASKVAQALAAKAG

Sequences:

>Translated_301_residues
MARPPSVRIVEVGPRDGLQNEPGVVPLAAKVALINALAEAGLTMIESGSFVSPKWVPQMADTAQVLAEIDRRDGVRYPVL
TPNLKGLELALASGVEEVAVFAAASEAFSQKNINCTIAESLERFAPVLDLAKAQGVAVRGYVSCVLGCPYQGEVPVAKVV
EVSERLIAMGCYEVSLGDTIGVGTPDKAKAMAQAVAGVIGVERTALHFHDTYGQALANVLACLELGIATVDAAAAGLGGC
PYAKGASGNLATEDLVYMLDGLGVDTGVDLDALARAGRAITAALSRTPASKVAQALAAKAG
>Mature_300_residues
ARPPSVRIVEVGPRDGLQNEPGVVPLAAKVALINALAEAGLTMIESGSFVSPKWVPQMADTAQVLAEIDRRDGVRYPVLT
PNLKGLELALASGVEEVAVFAAASEAFSQKNINCTIAESLERFAPVLDLAKAQGVAVRGYVSCVLGCPYQGEVPVAKVVE
VSERLIAMGCYEVSLGDTIGVGTPDKAKAMAQAVAGVIGVERTALHFHDTYGQALANVLACLELGIATVDAAAAGLGGCP
YAKGASGNLATEDLVYMLDGLGVDTGVDLDALARAGRAITAALSRTPASKVAQALAAKAG

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=292, Percent_Identity=59.9315068493151, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI109150427, Length=292, Percent_Identity=58.5616438356164, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI109150422, Length=292, Percent_Identity=58.5616438356164, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI260654708, Length=292, Percent_Identity=42.1232876712329, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17510563, Length=293, Percent_Identity=53.5836177474403, Blast_Score=312, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24582381, Length=289, Percent_Identity=62.2837370242215, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 30782; Mature: 30651

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPPSVRIVEVGPRDGLQNEPGVVPLAAKVALINALAEAGLTMIESGSFVSPKWVPQMA
CCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
DTAQVLAEIDRRDGVRYPVLTPNLKGLELALASGVEEVAVFAAASEAFSQKNINCTIAES
HHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHH
LERFAPVLDLAKAQGVAVRGYVSCVLGCPYQGEVPVAKVVEVSERLIAMGCYEVSLGDTI
HHHHHHHHHHHHHCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCEE
GVGTPDKAKAMAQAVAGVIGVERTALHFHDTYGQALANVLACLELGIATVDAAAAGLGGC
CCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYAKGASGNLATEDLVYMLDGLGVDTGVDLDALARAGRAITAALSRTPASKVAQALAAKA
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCC
G
C
>Mature Secondary Structure 
ARPPSVRIVEVGPRDGLQNEPGVVPLAAKVALINALAEAGLTMIESGSFVSPKWVPQMA
CCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
DTAQVLAEIDRRDGVRYPVLTPNLKGLELALASGVEEVAVFAAASEAFSQKNINCTIAES
HHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHH
LERFAPVLDLAKAQGVAVRGYVSCVLGCPYQGEVPVAKVVEVSERLIAMGCYEVSLGDTI
HHHHHHHHHHHHHCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCEE
GVGTPDKAKAMAQAVAGVIGVERTALHFHDTYGQALANVLACLELGIATVDAAAAGLGGC
CCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYAKGASGNLATEDLVYMLDGLGVDTGVDLDALARAGRAITAALSRTPASKVAQALAAKA
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]