Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 154245777

GI number: 154245777

Start: 2060406

End: 2061701

Strand: Direct

Name: trmE

Synonym: Xaut_1833

Alternate gene names: 154245777

Gene position: 2060406-2061701 (Clockwise)

Preceding gene: 154245775

Following gene: 154245778

Centisome position: 38.81

GC content: 71.76

Gene sequence:

>1296_bases
ATGACCGACACCCTCTTCGCCCTCTCGTCGGGCCGCCTGCCGGCCGGCGTGGCGGTGCTGCGCATCAGCGGTCCCGAGGC
GGCGGCGGCCGTATTGGCCCTGTGTGGCGTGCTGCCCCCGCCCCGCGTCGCCCGCTACGGCGCGCTCAGCGATCCACGGA
CCGGAGAGACGCTGGACCGCGGCCTCATCCTGTTCTTCCCCGGCCCCGCCAGCGCCACGGGGGAGGATGTGGCCGAGCTT
CATCTCCATGGCGGGCGCGCCGTGGTGGCGGCGGTGCTGCGGGCCTTGTCCGCCCTGCCCGGCCTGCGCCCGGCCGAGGC
CGGCGAATTCACCCGTAGAGCCCATGCCAACGGCAAGCTGGACCTGGCGGAGGTGGAGGGCCTGGCCGATCTCGTCGCTG
CGGAGAGCGAGGCGCAGCGCAAGCAGGCCTTGGCGCTGGCCTCCGGCGCCCTCTCCCGTCGGGTGGAGGAGTGGCGCGTG
GGGCTGGTCTCTGCCCTTGCCTTGATCGAGGCCGGCATCGATTTCTCGGACGAGAGCGACGTGTCGGGAGAGGTGACGAT
GGATGCCGTCGCCCGCCTCGCCAGGCTGAAGGAGGAATTGCTTGCCGCTCTCACCGACGCGGCGCGGGGCGAGCGGGTGC
GGGACGGTCTGGTGGTGGCCATTTCCGGCCCGCCCAATGCCGGAAAATCCACCCTCTTGAACCGCCTTGCCGGGCGCGAG
GCGGCCATCGTCTCCGCCCTTCCCGGCACCACCCGCGACGTGCTGGAGGTGCATCTGGAACTGGCCGGGCAGGCCGTGAC
CCTGCTGGACACCGCCGGCCTGCGTGAGACCTCCGACCTTGTGGAAGCCGAAGGCGTGCGTCGCGCCCTGGCGCGGGCGG
AAGGTGCGGACGTGGTGCTCTGGCTCTGCGATACCGGCTCGCAGCCGCCCCCTGCCCTCGCCCACGCCGTGAAGGTGCGC
ACCAAGATCGACCGGGATGCCGCCATTCCCTTAGGCTGGATCGGCATTTCCGCTGCCTCTGGAGCGGGCGTGGACACCCT
CGTCAGCCGGCTGGAACAGGAGGCGGACCGCCTCGGGGGGCGCGAGCCGGCGCTCATTACCCGCGAACGGCAGCGGCGGG
CGCTGGAGGTGGCCACGCTGCATCTGGAGCGGGCGCTGGGCGACTTCGGCGGCCATGAGGAATTGCGGGCCGAGGACGTG
CGCCTCGCGGTGCGGGCTCTGGACCAGCTCATCGGGCGGGTGGATGTGGAGGATGTGCTGGACGCCCTGTTCAGTACCTT
CTGCATCGGCAAATAG

Upstream 100 bases:

>100_bases
GGCAATGGAGATGGCGACGAAAAATTCAGGCAATGAAGGACGCATGGTCCGCCATCTGCCGATGCCGTGCAAATCCGTCG
CCAGTTTGGGAAACGACGCC

Downstream 100 bases:

>100_bases
GCGCCACCCGCGCCCGCAGGATGTTTCACGTGAAACATCGCCGGCTGCGGCCTTGACGGCGCCGGAAAGAGGGCGTTTCC
AGAAGGGGCGGCACGGACCG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTDTLFALSSGRLPAGVAVLRISGPEAAAAVLALCGVLPPPRVARYGALSDPRTGETLDRGLILFFPGPASATGEDVAEL
HLHGGRAVVAAVLRALSALPGLRPAEAGEFTRRAHANGKLDLAEVEGLADLVAAESEAQRKQALALASGALSRRVEEWRV
GLVSALALIEAGIDFSDESDVSGEVTMDAVARLARLKEELLAALTDAARGERVRDGLVVAISGPPNAGKSTLLNRLAGRE
AAIVSALPGTTRDVLEVHLELAGQAVTLLDTAGLRETSDLVEAEGVRRALARAEGADVVLWLCDTGSQPPPALAHAVKVR
TKIDRDAAIPLGWIGISAASGAGVDTLVSRLEQEADRLGGREPALITRERQRRALEVATLHLERALGDFGGHEELRAEDV
RLAVRALDQLIGRVDVEDVLDALFSTFCIGK

Sequences:

>Translated_431_residues
MTDTLFALSSGRLPAGVAVLRISGPEAAAAVLALCGVLPPPRVARYGALSDPRTGETLDRGLILFFPGPASATGEDVAEL
HLHGGRAVVAAVLRALSALPGLRPAEAGEFTRRAHANGKLDLAEVEGLADLVAAESEAQRKQALALASGALSRRVEEWRV
GLVSALALIEAGIDFSDESDVSGEVTMDAVARLARLKEELLAALTDAARGERVRDGLVVAISGPPNAGKSTLLNRLAGRE
AAIVSALPGTTRDVLEVHLELAGQAVTLLDTAGLRETSDLVEAEGVRRALARAEGADVVLWLCDTGSQPPPALAHAVKVR
TKIDRDAAIPLGWIGISAASGAGVDTLVSRLEQEADRLGGREPALITRERQRRALEVATLHLERALGDFGGHEELRAEDV
RLAVRALDQLIGRVDVEDVLDALFSTFCIGK
>Mature_430_residues
TDTLFALSSGRLPAGVAVLRISGPEAAAAVLALCGVLPPPRVARYGALSDPRTGETLDRGLILFFPGPASATGEDVAELH
LHGGRAVVAAVLRALSALPGLRPAEAGEFTRRAHANGKLDLAEVEGLADLVAAESEAQRKQALALASGALSRRVEEWRVG
LVSALALIEAGIDFSDESDVSGEVTMDAVARLARLKEELLAALTDAARGERVRDGLVVAISGPPNAGKSTLLNRLAGREA
AIVSALPGTTRDVLEVHLELAGQAVTLLDTAGLRETSDLVEAEGVRRALARAEGADVVLWLCDTGSQPPPALAHAVKVRT
KIDRDAAIPLGWIGISAASGAGVDTLVSRLEQEADRLGGREPALITRERQRRALEVATLHLERALGDFGGHEELRAEDVR
LAVRALDQLIGRVDVEDVLDALFSTFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=43.6559139784946, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI193082993, Length=446, Percent_Identity=44.8430493273543, Blast_Score=302, Evalue=5e-82,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=41.0821643286573, Blast_Score=292, Evalue=3e-79,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=40.9190371991247, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082120, Length=89, Percent_Identity=43.8202247191011, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=449, Percent_Identity=31.8485523385301, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=29.7959183673469, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=37.9237288135593, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 45126; Mature: 44995

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTLFALSSGRLPAGVAVLRISGPEAAAAVLALCGVLPPPRVARYGALSDPRTGETLDR
CCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHC
GLILFFPGPASATGEDVAELHLHGGRAVVAAVLRALSALPGLRPAEAGEFTRRAHANGKL
CEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCE
DLAEVEGLADLVAAESEAQRKQALALASGALSRRVEEWRVGLVSALALIEAGIDFSDESD
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VSGEVTMDAVARLARLKEELLAALTDAARGERVRDGLVVAISGPPNAGKSTLLNRLAGRE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCH
AAIVSALPGTTRDVLEVHLELAGQAVTLLDTAGLRETSDLVEAEGVRRALARAEGADVVL
HHHHCCCCCCHHHHHHHHHHHCCCEEEEHECCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
WLCDTGSQPPPALAHAVKVRTKIDRDAAIPLGWIGISAASGAGVDTLVSRLEQEADRLGG
EEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
REPALITRERQRRALEVATLHLERALGDFGGHEELRAEDVRLAVRALDQLIGRVDVEDVL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DALFSTFCIGK
HHHHHHHHCCC
>Mature Secondary Structure 
TDTLFALSSGRLPAGVAVLRISGPEAAAAVLALCGVLPPPRVARYGALSDPRTGETLDR
CCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHC
GLILFFPGPASATGEDVAELHLHGGRAVVAAVLRALSALPGLRPAEAGEFTRRAHANGKL
CEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCE
DLAEVEGLADLVAAESEAQRKQALALASGALSRRVEEWRVGLVSALALIEAGIDFSDESD
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VSGEVTMDAVARLARLKEELLAALTDAARGERVRDGLVVAISGPPNAGKSTLLNRLAGRE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCH
AAIVSALPGTTRDVLEVHLELAGQAVTLLDTAGLRETSDLVEAEGVRRALARAEGADVVL
HHHHCCCCCCHHHHHHHHHHHCCCEEEEHECCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
WLCDTGSQPPPALAHAVKVRTKIDRDAAIPLGWIGISAASGAGVDTLVSRLEQEADRLGG
EEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
REPALITRERQRRALEVATLHLERALGDFGGHEELRAEDVRLAVRALDQLIGRVDVEDVL
CCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DALFSTFCIGK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA