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The map label for this gene is mptN [H]

Identifier: 154245733

GI number: 154245733

Start: 2018265

End: 2019161

Strand: Direct

Name: mptN [H]

Synonym: Xaut_1789

Alternate gene names: 154245733

Gene position: 2018265-2019161 (Clockwise)

Preceding gene: 154245732

Following gene: 154245734

Centisome position: 38.02

GC content: 72.35

Gene sequence:

>897_bases
GTGGCGGGCCTCGTCCTCTTCGCCGAGCGGGTGGACTGGCACAGCCGCTCGCTCCTCGCCGCCATCAAGGCCCGCGGGCA
CACCGCGCGTGTCATCTCGCTGAAAGCCTGCGGCTTCGCCGTGGGGGAGACCGCCCACGGCCTGCTGCTGCCGGGCTTTG
CAGACGACCTGCCGGACGCCTGCTTCGTGCGCTCGGTGCCCGCCGGTACGCTGGAGCAGGTCACCGCCCGGCTTGGCGTG
CTCCATGCCCTGCGCGAGATGGGGGTGCGGGTGATGAACGACGCCCGCGCCATCGAGCGCTGCGTGGACAAGAGCGCCAC
CACCTTCCTTTTGGCCCGCGCCGGCCTCCCCACTCCCCGCACTTTGGTGCTGGAAGACCGCGCGGCGGCGCAGATGATCA
TCGACGCCGCGCCCGGCGACAGCGTGCTCAAGCCCATGTTCGGCGCCCAGGGCCGCGGCCTCATGCGCCTTGCACCCAAG
GCCCGCCTGCCGGAGGCCGAGGAGGTGGGCGGGGTCTATTACCTGCAGGATTTCGTTTCCGCGCCATCCGGCACCGGACC
CTCCGGGACCCATGAGGATTTCCGCGTGTTCGTGGTCGGCGGACAGGCCGAGGCCGCCATGGCGCGGCGGGGCACCTCCT
GGATCACCAACATCCACCAGGGCGCCACCGGCGTGGAAGTCGCCGCCGAGGGCCAGCTCGCGGACCTTGCCGTGCGCGCG
GCGGCGGCGGTGGGGGCGTCCTATGCGGGGGTGGACCTCATCGCCGATTCGGAAGGGCGCCTCACGGTGCTGGAGGTGAA
TTCCATGCCGGCCTGGCGCGGCCTTCATGACGCCACCGGCCGCAACATGGCCGAGCCGCTGGCCGCCCACCTCGTCGCCG
AGCCCGTGGCGGCGTAG

Upstream 100 bases:

>100_bases
TCCCCGGCGCGGGTGACGGTGACCGCCATCGACCATGGCGAGAGCTTCACCGCCGGCGGCTTCGATCCCATCCTCATCGC
CCGGTCGTTCGGGGGCTGAG

Downstream 100 bases:

>100_bases
GCTCCGGGCGGACTTTCGGCATTCAAGGAAAACCTGCGCATGCGCGTCTTCGTCTGCGAGTTCGTCACCGGCGGCGGCCT
GCGCGACGCGGCACTGCCGG

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAGLVLFAERVDWHSRSLLAAIKARGHTARVISLKACGFAVGETAHGLLLPGFADDLPDACFVRSVPAGTLEQVTARLGV
LHALREMGVRVMNDARAIERCVDKSATTFLLARAGLPTPRTLVLEDRAAAQMIIDAAPGDSVLKPMFGAQGRGLMRLAPK
ARLPEAEEVGGVYYLQDFVSAPSGTGPSGTHEDFRVFVVGGQAEAAMARRGTSWITNIHQGATGVEVAAEGQLADLAVRA
AAAVGASYAGVDLIADSEGRLTVLEVNSMPAWRGLHDATGRNMAEPLAAHLVAEPVAA

Sequences:

>Translated_298_residues
MAGLVLFAERVDWHSRSLLAAIKARGHTARVISLKACGFAVGETAHGLLLPGFADDLPDACFVRSVPAGTLEQVTARLGV
LHALREMGVRVMNDARAIERCVDKSATTFLLARAGLPTPRTLVLEDRAAAQMIIDAAPGDSVLKPMFGAQGRGLMRLAPK
ARLPEAEEVGGVYYLQDFVSAPSGTGPSGTHEDFRVFVVGGQAEAAMARRGTSWITNIHQGATGVEVAAEGQLADLAVRA
AAAVGASYAGVDLIADSEGRLTVLEVNSMPAWRGLHDATGRNMAEPLAAHLVAEPVAA
>Mature_297_residues
AGLVLFAERVDWHSRSLLAAIKARGHTARVISLKACGFAVGETAHGLLLPGFADDLPDACFVRSVPAGTLEQVTARLGVL
HALREMGVRVMNDARAIERCVDKSATTFLLARAGLPTPRTLVLEDRAAAQMIIDAAPGDSVLKPMFGAQGRGLMRLAPKA
RLPEAEEVGGVYYLQDFVSAPSGTGPSGTHEDFRVFVVGGQAEAAMARRGTSWITNIHQGATGVEVAAEGQLADLAVRAA
AAVGASYAGVDLIADSEGRLTVLEVNSMPAWRGLHDATGRNMAEPLAAHLVAEPVAA

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=279, Percent_Identity=26.1648745519713, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31162; Mature: 31031

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLVLFAERVDWHSRSLLAAIKARGHTARVISLKACGFAVGETAHGLLLPGFADDLPDA
CCCCEEEEHHHCHHHHHHHHHHHHCCCEEEEEEEEECCEECCCCCCCEECCCCHHCCCCH
CFVRSVPAGTLEQVTARLGVLHALREMGVRVMNDARAIERCVDKSATTFLLARAGLPTPR
HHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCC
TLVLEDRAAAQMIIDAAPGDSVLKPMFGAQGRGLMRLAPKARLPEAEEVGGVYYLQDFVS
EEEEECCCCEEEEEECCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCHHHHHHHC
APSGTGPSGTHEDFRVFVVGGQAEAAMARRGTSWITNIHQGATGVEVAAEGQLADLAVRA
CCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHCCCCCCEEEECCCHHHHHHHH
AAAVGASYAGVDLIADSEGRLTVLEVNSMPAWRGLHDATGRNMAEPLAAHLVAEPVAA
HHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGLVLFAERVDWHSRSLLAAIKARGHTARVISLKACGFAVGETAHGLLLPGFADDLPDA
CCCEEEEHHHCHHHHHHHHHHHHCCCEEEEEEEEECCEECCCCCCCEECCCCHHCCCCH
CFVRSVPAGTLEQVTARLGVLHALREMGVRVMNDARAIERCVDKSATTFLLARAGLPTPR
HHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCC
TLVLEDRAAAQMIIDAAPGDSVLKPMFGAQGRGLMRLAPKARLPEAEEVGGVYYLQDFVS
EEEEECCCCEEEEEECCCCCHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCHHHHHHHC
APSGTGPSGTHEDFRVFVVGGQAEAAMARRGTSWITNIHQGATGVEVAAEGQLADLAVRA
CCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHCCCCCCEEEECCCHHHHHHHH
AAAVGASYAGVDLIADSEGRLTVLEVNSMPAWRGLHDATGRNMAEPLAAHLVAEPVAA
HHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA