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The map label for this gene is dctA [H]

Identifier: 154245718

GI number: 154245718

Start: 1999138

End: 2000484

Strand: Reverse

Name: dctA [H]

Synonym: Xaut_1774

Alternate gene names: 154245718

Gene position: 2000484-1999138 (Counterclockwise)

Preceding gene: 154245721

Following gene: 154245717

Centisome position: 37.68

GC content: 65.4

Gene sequence:

>1347_bases
ATGGCCCATGCAATTCCCGCCGCGGCGGTGCCGGCGCATCCTCGCAAATTCTACCACCACCTGTATTTCCAGGTCATCAT
CGCCATTATCGCGGGCATCCTGCTCGGCCATTTCTACCCGGATGTGGGCGCCAGCCTGAAGCCGCTCGGCGACGCCTTCA
TCAAGCTCGTCAAGATGGTGATCGCCCCGGTCATCTTCCTCACCGTGGTCACCGGCATTGCCGGCATGCGGGACCTCGCC
AAGGTCGGGCGCGTGGCCGGCAAGGCCATGATCTACTTCATCACCTTCTCCACCCTGGCGCTGCTGATCGGCCTCGTGGT
GGCGAACGTGGTGCAGCCCGGCTCGGGCCTCAACATCGATCCCAAGTCGCTGGATCCCAAGGCGGTGGCCGGCTATGCGG
CGCAGGCCCATGATTCCACCATCATCGGCTTCCTGATGAACATCATCCCGACGACGCCCATCTCGGCGCTCGCCTCGGGC
GACATCCTGCAGGTGCTGTTCTTCGCCGTGCTGTTCGGCATCGCCCTGGGCGGCGTCGGCGAGGCCGGCGAGCCGGTGAT
GAAGATCCTGAACTCGGCCTCGGCGGCCATGTTCCGGCTGGTGGCGATCCTGATGAAGGCGGCCCCCCTCGGCGCGTTCG
GCGCCATGGCCTTCACCATCGGCAAATACGGCATCGGCTCGGTGGCGAACCTCGCCATGCTGATCGTCACCTTCTACATC
ACCGCGGCGCTGTTCATCTTCGTGGTGCTGGGCCTGGTGGCGCGCTACAACGGCTTCTCCCTGTGGAAGCTGTTGAAGTA
TATCCGCGAGGAACTGCTCCTCGTGCTCGGCACCTCCTCCTCTGAAGCCGCCCTGCCGGGCCTGATGCTGAAGATGGAAC
GCGCCGGCTGTGCCAAGTCGGTGGTGGGCCTCGTGATCCCCACGGGCTACTCGTTCAACCTCGATGGCACCAACATCTAC
ATGACGCTGGCGGCCCTGTTCATCGCGCAGGCCACCGGCATACACCTGTCGTTCGAGGAACAGATCCTGCTGCTTCTGGT
GGCCATGCTCTCGTCCAAGGGCGCGGCGGGCATCACCGGCGCCGGCTTCATCACCCTCGCCGCCACCCTCTCGGTGGTGC
CGAGCGTGCCGGTGGCGGGCCTCGCCCTCATCCTCGGCATCGACCGCTTCATGTCCGAGTGCCGGGCCATCACCAACTTC
ATCGGCAATGCGGTGGCCACCATCGTCGTCGCCCGCTGGGAAGGCGAGCTGAACAAGGACCAGCTCAGCCGTGCCCTCAA
CGGCGAGCACTTCTCCCTCGAGGACGAGCTGGAGACCACCGGCACCCCGGCCATCGCGGCCGAGTGA

Upstream 100 bases:

>100_bases
GGGGACGTACCCGGCGTCCTGCGCCATCCTGTCAACGAAGCATCCGGCGTCCGCAGTGCCCATCGCAAGAGGCACCCGGC
CCCGGCGGAGGAACTCTCCA

Downstream 100 bases:

>100_bases
GCAGGCCCTGATGACATCTGGCGGGCTCCGCCTCTCCGCGGAGAGGCGGGCGGGGGCCCGCCGGATCAGGACCGCAAGGT
CCGTCACCGCCTCCCCTCAG

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAHAIPAAAVPAHPRKFYHHLYFQVIIAIIAGILLGHFYPDVGASLKPLGDAFIKLVKMVIAPVIFLTVVTGIAGMRDLA
KVGRVAGKAMIYFITFSTLALLIGLVVANVVQPGSGLNIDPKSLDPKAVAGYAAQAHDSTIIGFLMNIIPTTPISALASG
DILQVLFFAVLFGIALGGVGEAGEPVMKILNSASAAMFRLVAILMKAAPLGAFGAMAFTIGKYGIGSVANLAMLIVTFYI
TAALFIFVVLGLVARYNGFSLWKLLKYIREELLLVLGTSSSEAALPGLMLKMERAGCAKSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATGIHLSFEEQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGLALILGIDRFMSECRAITNF
IGNAVATIVVARWEGELNKDQLSRALNGEHFSLEDELETTGTPAIAAE

Sequences:

>Translated_448_residues
MAHAIPAAAVPAHPRKFYHHLYFQVIIAIIAGILLGHFYPDVGASLKPLGDAFIKLVKMVIAPVIFLTVVTGIAGMRDLA
KVGRVAGKAMIYFITFSTLALLIGLVVANVVQPGSGLNIDPKSLDPKAVAGYAAQAHDSTIIGFLMNIIPTTPISALASG
DILQVLFFAVLFGIALGGVGEAGEPVMKILNSASAAMFRLVAILMKAAPLGAFGAMAFTIGKYGIGSVANLAMLIVTFYI
TAALFIFVVLGLVARYNGFSLWKLLKYIREELLLVLGTSSSEAALPGLMLKMERAGCAKSVVGLVIPTGYSFNLDGTNIY
MTLAALFIAQATGIHLSFEEQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGLALILGIDRFMSECRAITNF
IGNAVATIVVARWEGELNKDQLSRALNGEHFSLEDELETTGTPAIAAE
>Mature_447_residues
AHAIPAAAVPAHPRKFYHHLYFQVIIAIIAGILLGHFYPDVGASLKPLGDAFIKLVKMVIAPVIFLTVVTGIAGMRDLAK
VGRVAGKAMIYFITFSTLALLIGLVVANVVQPGSGLNIDPKSLDPKAVAGYAAQAHDSTIIGFLMNIIPTTPISALASGD
ILQVLFFAVLFGIALGGVGEAGEPVMKILNSASAAMFRLVAILMKAAPLGAFGAMAFTIGKYGIGSVANLAMLIVTFYIT
AALFIFVVLGLVARYNGFSLWKLLKYIREELLLVLGTSSSEAALPGLMLKMERAGCAKSVVGLVIPTGYSFNLDGTNIYM
TLAALFIAQATGIHLSFEEQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGLALILGIDRFMSECRAITNFI
GNAVATIVVARWEGELNKDQLSRALNGEHFSLEDELETTGTPAIAAE

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=467, Percent_Identity=27.1948608137045, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI40254478, Length=482, Percent_Identity=26.5560165975104, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI194239697, Length=462, Percent_Identity=23.1601731601732, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI5032093, Length=346, Percent_Identity=30.3468208092486, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI4827012, Length=288, Percent_Identity=29.5138888888889, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI223468566, Length=327, Percent_Identity=30.5810397553517, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI223468564, Length=296, Percent_Identity=32.0945945945946, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI21314632, Length=401, Percent_Identity=26.4339152119701, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI66773030, Length=274, Percent_Identity=29.9270072992701, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI262359914, Length=410, Percent_Identity=25.3658536585366, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI301601644, Length=149, Percent_Identity=29.5302013422819, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789947, Length=412, Percent_Identity=61.4077669902913, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1790514, Length=415, Percent_Identity=39.2771084337349, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1788024, Length=407, Percent_Identity=22.1130221130221, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789473, Length=226, Percent_Identity=28.3185840707965, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71996953, Length=447, Percent_Identity=27.5167785234899, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17537407, Length=448, Percent_Identity=25.4464285714286, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983099, Length=443, Percent_Identity=25.5079006772009, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71983106, Length=443, Percent_Identity=25.5079006772009, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=452, Percent_Identity=24.3362831858407, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=400, Percent_Identity=24.25, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=432, Percent_Identity=22.2222222222222, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583025, Length=427, Percent_Identity=25.7611241217799, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI17137668, Length=427, Percent_Identity=25.7611241217799, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24583023, Length=427, Percent_Identity=25.7611241217799, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI281360481, Length=489, Percent_Identity=24.9488752556237, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281360483, Length=489, Percent_Identity=24.9488752556237, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137666, Length=489, Percent_Identity=24.9488752556237, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46966; Mature: 46834

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AHAIPAAAVPAHPRKFYHHLYFQVIIAIIAGILLGHFYPDVGASLKPLGDAFIKLVKMV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IAPVIFLTVVTGIAGMRDLAKVGRVAGKAMIYFITFSTLALLIGLVVANVVQPGSGLNID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PKSLDPKAVAGYAAQAHDSTIIGFLMNIIPTTPISALASGDILQVLFFAVLFGIALGGVG
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCC
EAGEPVMKILNSASAAMFRLVAILMKAAPLGAFGAMAFTIGKYGIGSVANLAMLIVTFYI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TAALFIFVVLGLVARYNGFSLWKLLKYIREELLLVLGTSSSEAALPGLMLKMERAGCAKS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHH
VVGLVIPTGYSFNLDGTNIYMTLAALFIAQATGIHLSFEEQILLLLVAMLSSKGAAGITG
HHHHEECCCEEECCCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCCCCC
AGFITLAATLSVVPSVPVAGLALILGIDRFMSECRAITNFIGNAVATIVVARWEGELNKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QLSRALNGEHFSLEDELETTGTPAIAAE
HHHHHHCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA