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The map label for this gene is folC [H]

Identifier: 154245641

GI number: 154245641

Start: 1909226

End: 1910569

Strand: Direct

Name: folC [H]

Synonym: Xaut_1697

Alternate gene names: 154245641

Gene position: 1909226-1910569 (Clockwise)

Preceding gene: 154245640

Following gene: 154245643

Centisome position: 35.96

GC content: 72.32

Gene sequence:

>1344_bases
ATGAACATGGCCCTGCCCCGCCCCTTCCCCCTGCCCGCCCGCGCCACCGACGAGATCCTCACTCGCCTCGCCGCGCTTCA
CCCCAAGAAGATCGATCTGTCCCTGGCGCGGATGGGACGGCTGCTGGACGCCCTCGGCAATCCGGAGCGGCGGCTGCCGC
CGACGATCCATGTGGCCGGTACCAACGGCAAGGGCTCCACCATCGCCTTCATGCGCGCCATGCTGGAGGCGGCAGGCCAG
CGGGTGCACGTCTATACCTCGCCCCATCTGGTGCGCTTCAACGAACGCATCCGCCTCGGCGCGCCGGGGGGCGGCGTGCT
GGTATCGGACGCGGCCCTCGCCGAGGCCCTCGACCGGACCGAGACCGCCAATGCCGGCCAGCCCATCACCCTGTTCGAGA
TCACCACGGCGGCGGCCTTCCTGCTGTTCGCCGAGCATCCGGCCGACGTGCTGCTGCTGGAGGTGGGCCTCGGCGGCCGG
CTCGACGCCACCAATCTGGTGGCCGAGCCGCTGGCGAGCGTGATCACCCCCGTCTCCATCGACCATGTGGACTTCCTTGG
CACCTCGGTGGAGGCCATCGCCAGCGAGAAGGCGGGCATCATCAAGCCGCGCCGGCCGGTGGTGGTCGGCTCGCAGGTGC
CGGAGGCGCTCGCCGTCATCGAACGGGCGGCGGCGAAGAAGCTTGCGCCTTTGTTCGTGCGCGGCCAGCAGTGGAACGTC
AGCGAGGAAGCCGGCCGCCTCGTCTATGCCGACGAGGACGGCTTGCTGGACCTGCCGCGCCCGCGCCTCGTGGGACCGCA
CCAGATCGACAATGCCGGCCTTGCGGTGGCGACGCTGCGCGCCGTGAAGCTGCCGGCGGCCCATGAGGCCGGCATCCTCC
AGGCCGACTGGCCGGCGCGGCTCCAGCGCCTGCCCTCGGGGCCGCTGGTGGCGCGGGCGCCGGCCGGCGTGGAGGTGTGG
CTTGACGGCGGGCACAATGCCGCCGGCGGTGCCGCCCTCGCCCATGCTTTGTGCGAGCTTGAGGAGCGCTATTCGCGCCC
CCTCGTGCTCATCCTCGGCATGCTGGGCACCAAGGATGTCACCGGCTTCCTCACGCCCTTCGCCGGGCTGGTGAGGGAGG
CCATCGCCGTGCCGGTGCCCGGCGAGCATGCCGGCCTCAAGCCCGAGGACGTGGCCGCCGCCGCCATGGGCCTCGGCATG
TCGGCGCGGGTCGCCCGCGACGTGGGCAGCGCCCTCGACAGCATCACCCGCACCTCGCAGGTGCCGCCGCCGCGAGTGCT
GATCTCCGGCTCGCTCTATCTTGCCGGAGCGGTGCTCGCCGCCAACGGCGCCGAGCCGGCCTGA

Upstream 100 bases:

>100_bases
CGCCGGCCCTGCCACCCAAGGGCCGCCTGCCCCGCCCCGAAGCGGCCTGACAAGGACGCGCCAGCGGGCGCCGGCACTTC
GCCGCTGCGCCCGCGCGAGA

Downstream 100 bases:

>100_bases
CCACATCGCCTCACGCGAAGGCGAAAATGCTTGCGCCCTTTTCGGCCTGGCCGACGGCGTTTCGGAAGGGGGCGAAGAGC
TCGCGGCCGACGCCGGAGTG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNMALPRPFPLPARATDEILTRLAALHPKKIDLSLARMGRLLDALGNPERRLPPTIHVAGTNGKGSTIAFMRAMLEAAGQ
RVHVYTSPHLVRFNERIRLGAPGGGVLVSDAALAEALDRTETANAGQPITLFEITTAAAFLLFAEHPADVLLLEVGLGGR
LDATNLVAEPLASVITPVSIDHVDFLGTSVEAIASEKAGIIKPRRPVVVGSQVPEALAVIERAAAKKLAPLFVRGQQWNV
SEEAGRLVYADEDGLLDLPRPRLVGPHQIDNAGLAVATLRAVKLPAAHEAGILQADWPARLQRLPSGPLVARAPAGVEVW
LDGGHNAAGGAALAHALCELEERYSRPLVLILGMLGTKDVTGFLTPFAGLVREAIAVPVPGEHAGLKPEDVAAAAMGLGM
SARVARDVGSALDSITRTSQVPPPRVLISGSLYLAGAVLAANGAEPA

Sequences:

>Translated_447_residues
MNMALPRPFPLPARATDEILTRLAALHPKKIDLSLARMGRLLDALGNPERRLPPTIHVAGTNGKGSTIAFMRAMLEAAGQ
RVHVYTSPHLVRFNERIRLGAPGGGVLVSDAALAEALDRTETANAGQPITLFEITTAAAFLLFAEHPADVLLLEVGLGGR
LDATNLVAEPLASVITPVSIDHVDFLGTSVEAIASEKAGIIKPRRPVVVGSQVPEALAVIERAAAKKLAPLFVRGQQWNV
SEEAGRLVYADEDGLLDLPRPRLVGPHQIDNAGLAVATLRAVKLPAAHEAGILQADWPARLQRLPSGPLVARAPAGVEVW
LDGGHNAAGGAALAHALCELEERYSRPLVLILGMLGTKDVTGFLTPFAGLVREAIAVPVPGEHAGLKPEDVAAAAMGLGM
SARVARDVGSALDSITRTSQVPPPRVLISGSLYLAGAVLAANGAEPA
>Mature_447_residues
MNMALPRPFPLPARATDEILTRLAALHPKKIDLSLARMGRLLDALGNPERRLPPTIHVAGTNGKGSTIAFMRAMLEAAGQ
RVHVYTSPHLVRFNERIRLGAPGGGVLVSDAALAEALDRTETANAGQPITLFEITTAAAFLLFAEHPADVLLLEVGLGGR
LDATNLVAEPLASVITPVSIDHVDFLGTSVEAIASEKAGIIKPRRPVVVGSQVPEALAVIERAAAKKLAPLFVRGQQWNV
SEEAGRLVYADEDGLLDLPRPRLVGPHQIDNAGLAVATLRAVKLPAAHEAGILQADWPARLQRLPSGPLVARAPAGVEVW
LDGGHNAAGGAALAHALCELEERYSRPLVLILGMLGTKDVTGFLTPFAGLVREAIAVPVPGEHAGLKPEDVAAAAMGLGM
SARVARDVGSALDSITRTSQVPPPRVLISGSLYLAGAVLAANGAEPA

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=368, Percent_Identity=29.8913043478261, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI66932990, Length=368, Percent_Identity=29.8913043478261, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1788654, Length=401, Percent_Identity=35.1620947630923, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17553148, Length=450, Percent_Identity=26.4444444444444, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553150, Length=450, Percent_Identity=26.4444444444444, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=448, Percent_Identity=26.5625, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=435, Percent_Identity=28.5057471264368, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6324815, Length=340, Percent_Identity=27.0588235294118, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24641571, Length=435, Percent_Identity=25.5172413793103, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24581568, Length=175, Percent_Identity=33.1428571428571, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46704; Mature: 46704

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMALPRPFPLPARATDEILTRLAALHPKKIDLSLARMGRLLDALGNPERRLPPTIHVAG
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TNGKGSTIAFMRAMLEAAGQRVHVYTSPHLVRFNERIRLGAPGGGVLVSDAALAEALDRT
CCCCCHHHHHHHHHHHHCCCEEEEEECCEEEEECCEEEEECCCCCEEEEHHHHHHHHHHH
ETANAGQPITLFEITTAAAFLLFAEHPADVLLLEVGLGGRLDATNLVAEPLASVITPVSI
CCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
DHVDFLGTSVEAIASEKAGIIKPRRPVVVGSQVPEALAVIERAAAKKLAPLFVRGQQWNV
CHHHHHCCHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCCC
SEEAGRLVYADEDGLLDLPRPRLVGPHQIDNAGLAVATLRAVKLPAAHEAGILQADWPAR
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCHHH
LQRLPSGPLVARAPAGVEVWLDGGHNAAGGAALAHALCELEERYSRPLVLILGMLGTKDV
HHHCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCH
TGFLTPFAGLVREAIAVPVPGEHAGLKPEDVAAAAMGLGMSARVARDVGSALDSITRTSQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
VPPPRVLISGSLYLAGAVLAANGAEPA
CCCCEEEEECCHHHHEEEEECCCCCCC
>Mature Secondary Structure
MNMALPRPFPLPARATDEILTRLAALHPKKIDLSLARMGRLLDALGNPERRLPPTIHVAG
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEE
TNGKGSTIAFMRAMLEAAGQRVHVYTSPHLVRFNERIRLGAPGGGVLVSDAALAEALDRT
CCCCCHHHHHHHHHHHHCCCEEEEEECCEEEEECCEEEEECCCCCEEEEHHHHHHHHHHH
ETANAGQPITLFEITTAAAFLLFAEHPADVLLLEVGLGGRLDATNLVAEPLASVITPVSI
CCCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
DHVDFLGTSVEAIASEKAGIIKPRRPVVVGSQVPEALAVIERAAAKKLAPLFVRGQQWNV
CHHHHHCCHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCCC
SEEAGRLVYADEDGLLDLPRPRLVGPHQIDNAGLAVATLRAVKLPAAHEAGILQADWPAR
HHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCHHH
LQRLPSGPLVARAPAGVEVWLDGGHNAAGGAALAHALCELEERYSRPLVLILGMLGTKDV
HHHCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCH
TGFLTPFAGLVREAIAVPVPGEHAGLKPEDVAAAAMGLGMSARVARDVGSALDSITRTSQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
VPPPRVLISGSLYLAGAVLAANGAEPA
CCCCEEEEECCHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]