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The map label for this gene is miaA

Identifier: 154245603

GI number: 154245603

Start: 1878404

End: 1879354

Strand: Reverse

Name: miaA

Synonym: Xaut_1659

Alternate gene names: 154245603

Gene position: 1879354-1878404 (Counterclockwise)

Preceding gene: 154245605

Following gene: 154245598

Centisome position: 35.4

GC content: 70.77

Gene sequence:

>951_bases
ATGGCAGTATCGGTACTCGAACAGGGGAATGGGCCGCAGGCGGTGCTCATCGCAGGTCCCACCGCCAGCGGCAAGTCGGC
CCTCGCGATGGCGCTCGCCGAGCGACTCACGGGCACCGTGCTCAATGCAGATTCCATGCAAGTCTATGGCGATCTTCGGG
TCCTCACGGCGCGGCCGACCGTCGCGGAGGCGGCGCGGGTGCCCCACGGCCTCTATGGCCACGTGGATGCGGACACCGAC
TATTCCGTGGGCCGCTGGCTCGCGGATGCCACGGCCGCCCTCGCTGCCGCGCGCGAACAGGGGCTTTTGCCCATCTTCGT
CGGCGGCACCGGCCTCTATTTCCGCGCGCTGACGCAGGGCCTCGCCGAGATCCCGCCGGTGCCGGAGACAGTGCGGCGCG
AGGTTCGCGGCACGGCGGAAGGCCTCGACAGCCTTGCCCTGCATGCCCGCCTTGCAGACATGGACCCGCAGAGCGCCGCC
CGGCTCCAGCCCAACGACCGTCAGCGGGTGTTGCGGGCACTGGAGGTAATCACCGCCACCGGCCGCTCGCTTTCAGACTG
GCAGAAGGATGCGCCGCGCCCGGTGCTGGATGCCTCCCGCTGCGTGCGTCTCGTGCTGGAGGTGGACCGCGCGGTGCTGC
GACAGCGCATCGATGCGCGCTTCGAGGCCATGATGGCTGAGGGGGCGCTGGAGGAGGTTCGCGCCCTGGCCGCGCGCCGG
CTCGATCCCTCGCGCACGGTGCTGAAGGCCCATGGCGCCCCGGCCCTCACCCGCCATCTCGCCGGCGAGATGACGCTGGA
CGACGCCATCGCCGAAGGCCAGAGCGACACCCGCCGTTACGCCAAGCGGCAGGAGACCTTCTTCCGCCACCAGATGGCGG
ACTGGCCCCGTGCCACACCGGAGGCGGCCTTGGATGCACTGCTCGGCATGATCAGGCCCCACGCGCGCTGA

Upstream 100 bases:

>100_bases
GGCCTCCTGCGCTTCGGGCAGCACCGCGCGCGCATCGTGGATTGCCTCCCGGGTCAAAGCCGGGGCAGATGGGTTTGAGA
TCAGCGTCGCGACATAGGCC

Downstream 100 bases:

>100_bases
GGCGGCGAAGGGCTCAGCGTCCTTCGGGGAGCGTCCCCTCCCCTTCCGCCAGAAGCTTTTTGAAGCGGGCTTCCTCGTCG
GCGGAGAGGCTCTGGGGGGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAVSVLEQGNGPQAVLIAGPTASGKSALAMALAERLTGTVLNADSMQVYGDLRVLTARPTVAEAARVPHGLYGHVDADTD
YSVGRWLADATAALAAAREQGLLPIFVGGTGLYFRALTQGLAEIPPVPETVRREVRGTAEGLDSLALHARLADMDPQSAA
RLQPNDRQRVLRALEVITATGRSLSDWQKDAPRPVLDASRCVRLVLEVDRAVLRQRIDARFEAMMAEGALEEVRALAARR
LDPSRTVLKAHGAPALTRHLAGEMTLDDAIAEGQSDTRRYAKRQETFFRHQMADWPRATPEAALDALLGMIRPHAR

Sequences:

>Translated_316_residues
MAVSVLEQGNGPQAVLIAGPTASGKSALAMALAERLTGTVLNADSMQVYGDLRVLTARPTVAEAARVPHGLYGHVDADTD
YSVGRWLADATAALAAAREQGLLPIFVGGTGLYFRALTQGLAEIPPVPETVRREVRGTAEGLDSLALHARLADMDPQSAA
RLQPNDRQRVLRALEVITATGRSLSDWQKDAPRPVLDASRCVRLVLEVDRAVLRQRIDARFEAMMAEGALEEVRALAARR
LDPSRTVLKAHGAPALTRHLAGEMTLDDAIAEGQSDTRRYAKRQETFFRHQMADWPRATPEAALDALLGMIRPHAR
>Mature_315_residues
AVSVLEQGNGPQAVLIAGPTASGKSALAMALAERLTGTVLNADSMQVYGDLRVLTARPTVAEAARVPHGLYGHVDADTDY
SVGRWLADATAALAAAREQGLLPIFVGGTGLYFRALTQGLAEIPPVPETVRREVRGTAEGLDSLALHARLADMDPQSAAR
LQPNDRQRVLRALEVITATGRSLSDWQKDAPRPVLDASRCVRLVLEVDRAVLRQRIDARFEAMMAEGALEEVRALAARRL
DPSRTVLKAHGAPALTRHLAGEMTLDDAIAEGQSDTRRYAKRQETFFRHQMADWPRATPEAALDALLGMIRPHAR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=352, Percent_Identity=29.2613636363636, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1790613, Length=316, Percent_Identity=34.4936708860759, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25144712, Length=240, Percent_Identity=27.5, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=298, Percent_Identity=28.5234899328859, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24649830, Length=235, Percent_Identity=32.7659574468085, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_XANP2 (A7IFW1)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416561.1
- ProteinModelPortal:   A7IFW1
- SMR:   A7IFW1
- STRING:   A7IFW1
- GeneID:   5424649
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_1659
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   XAUT78245:XAUT_1659-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34183; Mature: 34052

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSVLEQGNGPQAVLIAGPTASGKSALAMALAERLTGTVLNADSMQVYGDLRVLTARPT
CCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECEEEEECCCC
VAEAARVPHGLYGHVDADTDYSVGRWLADATAALAAAREQGLLPIFVGGTGLYFRALTQG
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
LAEIPPVPETVRREVRGTAEGLDSLALHARLADMDPQSAARLQPNDRQRVLRALEVITAT
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHC
GRSLSDWQKDAPRPVLDASRCVRLVLEVDRAVLRQRIDARFEAMMAEGALEEVRALAARR
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDPSRTVLKAHGAPALTRHLAGEMTLDDAIAEGQSDTRRYAKRQETFFRHQMADWPRATP
CCCCHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCH
EAALDALLGMIRPHAR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AVSVLEQGNGPQAVLIAGPTASGKSALAMALAERLTGTVLNADSMQVYGDLRVLTARPT
CEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECEEEEECCCC
VAEAARVPHGLYGHVDADTDYSVGRWLADATAALAAAREQGLLPIFVGGTGLYFRALTQG
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
LAEIPPVPETVRREVRGTAEGLDSLALHARLADMDPQSAARLQPNDRQRVLRALEVITAT
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHC
GRSLSDWQKDAPRPVLDASRCVRLVLEVDRAVLRQRIDARFEAMMAEGALEEVRALAARR
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDPSRTVLKAHGAPALTRHLAGEMTLDDAIAEGQSDTRRYAKRQETFFRHQMADWPRATP
CCCCHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCH
EAALDALLGMIRPHAR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA