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The map label for this gene is thrS

Identifier: 154245583

GI number: 154245583

Start: 1856211

End: 1858193

Strand: Reverse

Name: thrS

Synonym: Xaut_1638

Alternate gene names: 154245583

Gene position: 1858193-1856211 (Counterclockwise)

Preceding gene: 154245590

Following gene: 154245580

Centisome position: 35.0

GC content: 65.81

Gene sequence:

>1983_bases
ATGATTTCCCTGACCTTCCCCGACGGCGCCAAGCGCGACTATGCCGAGGGGACGTCCGGCGCGGACATCGCCGCCGCCAT
TTCCAAGTCACTCGCCAAGAAGGCCGTCGCCGTCACCCTCGACGGTGCGCTCACCGACCTGTCCGAGCCCATCACCCGCG
ACGCCAAGCTGGAGATCGTCACCCGCGACGACCCGCGCGCGCTGGAGCTGATCCGCCATGACTGCTCGCACGTCATGGCG
GAGGCGGTGCAGGAGCTGTGGCCGGGCACGCAGGTGACCATCGGCCCGGTGATCGAGAACGGCTTTTATTACGACTTCGC
CCGCAACGCCCCCTTCACCACCGAAGACCTGCCGGTCATCGAGAACAAGATGCGCGAGATCATCGCCCGCGACCGCCCCT
TCACCAAGGAGGTGTGGACCCGCGACGAAGCCAAGCGCGTGTTCGCCGCCAAGGGCGAGGGCTACAAGGTCGAGCTGGTG
GATGCCATCCCGTCCGACCAGGACGTGAAGATCTACAAGCAGGGCGAGTGGTTCGACCTCTGCCGCGGCCCGCACATGCC
CTCCACGGGCAAGATCGGCACCGCCTTCAAGCTGATGAAGGTGGCGGGCGCCTATTGGCGCGGCGATTCGAACAACCCCA
TGCTCACCCGCATCTACGGCACAGCCTGGCAGGACGAGAAGGAGCTCAACGCCTACATCCATCGACTGGAGGAGGCGGAG
AAGCGCGACCATCGCCGCCTCGGCCGCGAGATGGACCTGTTCCACTTCCAGGAGGAAGGCCCCGGTACGGTATTTTGGCA
CCCCAAGGGCTGGACCCTGTTCCAGGAACTGGTGGCCTACATGCGCCGGCGCCTGAAGGCCGACTACAACGAGGTCAACG
CCCCGCAGGTACTCGACGCCGGCCTGTGGGAGACCTCCGGCCACTGGGGCTGGTACAAGGAAAACATGTTCAAGGTGCAG
TCCGCGGGCGACGAGACCAAGGACGAGCGCGTGTTCGCCTTGAAGCCCATGAACTGCCCCGGCCATGTGCAGATCTTCAA
GCACGGTCTTAAGAGTTACCGCGATCTACCTCTGCGCCTTGCGGAATTCGGCGCGGTGCATCGCTACGAACCGTCCGGTG
CGCTGCATGGCCTCATGCGCGTGCGCGGCTTCACCCAGGACGACGCCCACGTCTTCTGCACCGAGGAGCAGATGGCGGCC
GAGTGCCTGAAGATCAACGACCTGATCCTCTCCACCTATGCCGATTTCGGCTTCGACGAGATCGTCGTGAAGCTATCCAC
GCGGCCGGAGAAGCGCGTGGGCTCGGATGCGGTGTGGGATCACGCCGAGGAGGTGATGACCCGCGTGCTCGCCGAGATCG
AGGCGGCCTCGGGCGGGCGCATCAAGACCGGCATCCTGCCCGGCGAGGGCGCCTTCTACGGCCCCAAGTTCGAATACACC
CTGAAGGACGCCATCGGCCGTGAATGGCAGTGCGGTACCACCCAGGTAGACTTCAACCTGCCCGAGCGGTTCGGCGCCTT
CTACGTGGACGCGGACGGCCAGAAGAAGACTCCGGTGATGATCCACCGCGCCATCTGCGGCTCCATGGAGCGCTTCACCG
GCATCCTCATCGAGCATTTCGCCGGCCACTTCCCGCTCTGGCTCGCCCCCACGCAGGTGGTGGTGGCCACCATCACCTCG
GATGCGGACGACTATGCCGCCGAGGTGGCGGAGCTGCTGAAGGCGGAAGGCCTGCGCGTGGAACTCGACGTGCGCAACGA
GAAGATCAACCTGAAGGTGCGCGAGCACTCCCTCGCCAAGGTTCCGGTGCTGCTGGTGGCGGGCAAGAAGGAGGCGGCCG
AGCGCACCCTCTCTATCCGCCGCCTCGGCTCCAATGCCCAGACCGGCGGAACGCTGGACGAGGTGGTGGCGGCGCTGGTG
GATGAGGCCGTGCCGCCGGACCTCAAGCGCAAGGCTCTGGCCAAGGCCGAACGGGCGGCCTGA

Upstream 100 bases:

>100_bases
TTCTGGACGCGGGCGCCCGCTCTCGCTTAAAAGACCCGCGTGACCATGAAAAGACGCCTACCTGCGTGGTCCGCAGGAGT
GGGCGGCAAGGAGTTGCCTC

Downstream 100 bases:

>100_bases
GCACGCTGCACGCGAACGAAAAAGGCGGGAGGCCCAGGCCTCCCGCCTTTTTTCTTGGACGCTATGGGGCCGCGCGGAGG
CCTATTTCCTGAGGTTGGCG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MISLTFPDGAKRDYAEGTSGADIAAAISKSLAKKAVAVTLDGALTDLSEPITRDAKLEIVTRDDPRALELIRHDCSHVMA
EAVQELWPGTQVTIGPVIENGFYYDFARNAPFTTEDLPVIENKMREIIARDRPFTKEVWTRDEAKRVFAAKGEGYKVELV
DAIPSDQDVKIYKQGEWFDLCRGPHMPSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWQDEKELNAYIHRLEEAE
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQELVAYMRRRLKADYNEVNAPQVLDAGLWETSGHWGWYKENMFKVQ
SAGDETKDERVFALKPMNCPGHVQIFKHGLKSYRDLPLRLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHVFCTEEQMAA
ECLKINDLILSTYADFGFDEIVVKLSTRPEKRVGSDAVWDHAEEVMTRVLAEIEAASGGRIKTGILPGEGAFYGPKFEYT
LKDAIGREWQCGTTQVDFNLPERFGAFYVDADGQKKTPVMIHRAICGSMERFTGILIEHFAGHFPLWLAPTQVVVATITS
DADDYAAEVAELLKAEGLRVELDVRNEKINLKVREHSLAKVPVLLVAGKKEAAERTLSIRRLGSNAQTGGTLDEVVAALV
DEAVPPDLKRKALAKAERAA

Sequences:

>Translated_660_residues
MISLTFPDGAKRDYAEGTSGADIAAAISKSLAKKAVAVTLDGALTDLSEPITRDAKLEIVTRDDPRALELIRHDCSHVMA
EAVQELWPGTQVTIGPVIENGFYYDFARNAPFTTEDLPVIENKMREIIARDRPFTKEVWTRDEAKRVFAAKGEGYKVELV
DAIPSDQDVKIYKQGEWFDLCRGPHMPSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWQDEKELNAYIHRLEEAE
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQELVAYMRRRLKADYNEVNAPQVLDAGLWETSGHWGWYKENMFKVQ
SAGDETKDERVFALKPMNCPGHVQIFKHGLKSYRDLPLRLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHVFCTEEQMAA
ECLKINDLILSTYADFGFDEIVVKLSTRPEKRVGSDAVWDHAEEVMTRVLAEIEAASGGRIKTGILPGEGAFYGPKFEYT
LKDAIGREWQCGTTQVDFNLPERFGAFYVDADGQKKTPVMIHRAICGSMERFTGILIEHFAGHFPLWLAPTQVVVATITS
DADDYAAEVAELLKAEGLRVELDVRNEKINLKVREHSLAKVPVLLVAGKKEAAERTLSIRRLGSNAQTGGTLDEVVAALV
DEAVPPDLKRKALAKAERAA
>Mature_660_residues
MISLTFPDGAKRDYAEGTSGADIAAAISKSLAKKAVAVTLDGALTDLSEPITRDAKLEIVTRDDPRALELIRHDCSHVMA
EAVQELWPGTQVTIGPVIENGFYYDFARNAPFTTEDLPVIENKMREIIARDRPFTKEVWTRDEAKRVFAAKGEGYKVELV
DAIPSDQDVKIYKQGEWFDLCRGPHMPSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWQDEKELNAYIHRLEEAE
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQELVAYMRRRLKADYNEVNAPQVLDAGLWETSGHWGWYKENMFKVQ
SAGDETKDERVFALKPMNCPGHVQIFKHGLKSYRDLPLRLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHVFCTEEQMAA
ECLKINDLILSTYADFGFDEIVVKLSTRPEKRVGSDAVWDHAEEVMTRVLAEIEAASGGRIKTGILPGEGAFYGPKFEYT
LKDAIGREWQCGTTQVDFNLPERFGAFYVDADGQKKTPVMIHRAICGSMERFTGILIEHFAGHFPLWLAPTQVVVATITS
DADDYAAEVAELLKAEGLRVELDVRNEKINLKVREHSLAKVPVLLVAGKKEAAERTLSIRRLGSNAQTGGTLDEVVAALV
DEAVPPDLKRKALAKAERAA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=607, Percent_Identity=39.5387149917628, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI38202255, Length=644, Percent_Identity=38.8198757763975, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI20070344, Length=656, Percent_Identity=38.109756097561, Blast_Score=427, Evalue=1e-119,
Organism=Escherichia coli, GI1788013, Length=652, Percent_Identity=50.920245398773, Blast_Score=684, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=646, Percent_Identity=38.6996904024768, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984184, Length=646, Percent_Identity=38.6996904024768, Blast_Score=456, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322112, Length=663, Percent_Identity=38.0090497737557, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322654, Length=402, Percent_Identity=37.0646766169154, Blast_Score=274, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24583842, Length=642, Percent_Identity=38.4735202492212, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI28574141, Length=642, Percent_Identity=38.4735202492212, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24583839, Length=642, Percent_Identity=38.4735202492212, Blast_Score=452, Evalue=1e-127,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73988; Mature: 73988

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLTFPDGAKRDYAEGTSGADIAAAISKSLAKKAVAVTLDGALTDLSEPITRDAKLEIV
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCEEEEE
TRDDPRALELIRHDCSHVMAEAVQELWPGTQVTIGPVIENGFYYDFARNAPFTTEDLPVI
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCCCCHHH
ENKMREIIARDRPFTKEVWTRDEAKRVFAAKGEGYKVELVDAIPSDQDVKIYKQGEWFDL
HHHHHHHHHCCCCCHHHHHCCHHHHHHEEECCCCEEEEEEECCCCCCCEEEEECCCCHHH
CRGPHMPSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWQDEKELNAYIHRLEEAE
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQELVAYMRRRLKADYNEVNAPQVLDA
HHHHHHHCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHC
GLWETSGHWGWYKENMFKVQSAGDETKDERVFALKPMNCPGHVQIFKHGLKSYRDLPLRL
CCCCCCCCCCCHHCCEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHH
AEFGAVHRYEPSGALHGLMRVRGFTQDDAHVFCTEEQMAAECLKINDLILSTYADFGFDE
HHHCCCEEECCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
IVVKLSTRPEKRVGSDAVWDHAEEVMTRVLAEIEAASGGRIKTGILPGEGAFYGPKFEYT
EEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEE
LKDAIGREWQCGTTQVDFNLPERFGAFYVDADGQKKTPVMIHRAICGSMERFTGILIEHF
HHHHCCCCCCCCCEEEECCCHHHHCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
AGHFPLWLAPTQVVVATITSDADDYAAEVAELLKAEGLRVELDVRNEKINLKVREHSLAK
CCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCC
VPVLLVAGKKEAAERTLSIRRLGSNAQTGGTLDEVVAALVDEAVPPDLKRKALAKAERAA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MISLTFPDGAKRDYAEGTSGADIAAAISKSLAKKAVAVTLDGALTDLSEPITRDAKLEIV
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCEEEEE
TRDDPRALELIRHDCSHVMAEAVQELWPGTQVTIGPVIENGFYYDFARNAPFTTEDLPVI
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCCCCCCCCHHH
ENKMREIIARDRPFTKEVWTRDEAKRVFAAKGEGYKVELVDAIPSDQDVKIYKQGEWFDL
HHHHHHHHHCCCCCHHHHHCCHHHHHHEEECCCCEEEEEEECCCCCCCEEEEECCCCHHH
CRGPHMPSTGKIGTAFKLMKVAGAYWRGDSNNPMLTRIYGTAWQDEKELNAYIHRLEEAE
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQELVAYMRRRLKADYNEVNAPQVLDA
HHHHHHHCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHC
GLWETSGHWGWYKENMFKVQSAGDETKDERVFALKPMNCPGHVQIFKHGLKSYRDLPLRL
CCCCCCCCCCCHHCCEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHH
AEFGAVHRYEPSGALHGLMRVRGFTQDDAHVFCTEEQMAAECLKINDLILSTYADFGFDE
HHHCCCEEECCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
IVVKLSTRPEKRVGSDAVWDHAEEVMTRVLAEIEAASGGRIKTGILPGEGAFYGPKFEYT
EEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEE
LKDAIGREWQCGTTQVDFNLPERFGAFYVDADGQKKTPVMIHRAICGSMERFTGILIEHF
HHHHCCCCCCCCCEEEECCCHHHHCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
AGHFPLWLAPTQVVVATITSDADDYAAEVAELLKAEGLRVELDVRNEKINLKVREHSLAK
CCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCC
VPVLLVAGKKEAAERTLSIRRLGSNAQTGGTLDEVVAALVDEAVPPDLKRKALAKAERAA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]