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The map label for this gene is pks2 [H]

Identifier: 154245566

GI number: 154245566

Start: 1835780

End: 1836790

Strand: Direct

Name: pks2 [H]

Synonym: Xaut_1621

Alternate gene names: 154245566

Gene position: 1835780-1836790 (Clockwise)

Preceding gene: 154245549

Following gene: 154245567

Centisome position: 34.58

GC content: 68.64

Gene sequence:

>1011_bases
ATGCCGGTGGTTGAAATTCCCAGTTCCATGCGCCAGGCGCGGTTCACCGGTGCCGGTGGACCGGAGGTCATTGCCATCGA
GACCGCGCCGGTGCCGCGTCCCGGGCCGGGGCAAGTGCTGGTGGAGGTGGTGGCCGCGGGCGTCAATCGCCCCGACTGCC
TCCAGCGCGCGGGCAATTATCCCCCGCCCCCCGGCGCCACGGAGATCCCGGGCCTCGAAATCGCGGGCCGTGTCGTCGCG
CTCGGCGAGGGCGTTGATTCCCTCAAGATCGGCGACGAAGTCTGCGCCCTGGTGATCTCGGGCGGCTACGCCGAGTATTG
CGTGGCGGAAGCCCCGCTCTGCCTGCCCATTCCCAAGCCGCTTTCGCTGCTTGAAGCCGCCGGGCTGCCGGAGAACTATT
ACACCGTCTACGACAACGTCTTCACCCGTGGCCGCCTGAAGGCGGGCGAGACCATCCTCATCCATGGCGGGTCGAGCGGC
ATCGGCTCTACCGCCATCCAGCTGGCGCACCATGCCGGCGCGCGCGTCATCGCCACCGCCGGCTCGCCGGAGAAGTGCGA
CTTCTGCCTGAAGCTCGGCGCGGACGCCGCCATCGACTATCGCGCCAAGGACTTCGTCGCCGAGGTGAAGGCGCTGACCG
ACGGGCGCGGCGTGGACGAGATCCTGGACATGGTGGGCGGGCCCTACGTGGCGCGGAACCTCCAGGCGCTGGCCGTGGAA
GGGCGGCTGGTACAGATCGCCTTCCTCCAGCCGAGCGAGGTCACCATCGACCTCATGCCGGTGATGCTGAAGCGCCTGAC
CTTTACCGGCTCGACCCTCCGCGCCCGCTCCGTGGCGCTGAAATCCGCCATCGCCGAAGCGCTGAAGCGGGACGTGTGGC
CGCTGCTGGAGGCGGGTAAGATCAAGCCCTTCGTCCACGCCACCTTCCCGCTGGAACAGGCGCGGGAGGCCCATGCGCTC
ATGGAATCGAGCGCCCACCTCGGCAAGATCATGCTGGAGACCGGACGGTGA

Upstream 100 bases:

>100_bases
CGGCCAAGTGCCGCGCGGCAGGTTCTGAACGCCACAAAAGACAGCGCACCCCCCGGATCTGCCAGCGATGCAGGACGATG
GGCGCGTGACCTAGGGAGGA

Downstream 100 bases:

>100_bases
GCCTGTCGTCTTCCCCCGTCGTTTTCGTCACCGGAGCAGGGCGCGGCATCGGCCGCGCCATGGCGGATGCCCATGCGGAT
GCCGGGGCCGCCGTCGCCTA

Product: quinone oxidoreductase putative PIG3

Products: NADP(+); Semiquinone. [C]

Alternate protein names: Polyketide synthase pks2 [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPVVEIPSSMRQARFTGAGGPEVIAIETAPVPRPGPGQVLVEVVAAGVNRPDCLQRAGNYPPPPGATEIPGLEIAGRVVA
LGEGVDSLKIGDEVCALVISGGYAEYCVAEAPLCLPIPKPLSLLEAAGLPENYYTVYDNVFTRGRLKAGETILIHGGSSG
IGSTAIQLAHHAGARVIATAGSPEKCDFCLKLGADAAIDYRAKDFVAEVKALTDGRGVDEILDMVGGPYVARNLQALAVE
GRLVQIAFLQPSEVTIDLMPVMLKRLTFTGSTLRARSVALKSAIAEALKRDVWPLLEAGKIKPFVHATFPLEQAREAHAL
MESSAHLGKIMLETGR

Sequences:

>Translated_336_residues
MPVVEIPSSMRQARFTGAGGPEVIAIETAPVPRPGPGQVLVEVVAAGVNRPDCLQRAGNYPPPPGATEIPGLEIAGRVVA
LGEGVDSLKIGDEVCALVISGGYAEYCVAEAPLCLPIPKPLSLLEAAGLPENYYTVYDNVFTRGRLKAGETILIHGGSSG
IGSTAIQLAHHAGARVIATAGSPEKCDFCLKLGADAAIDYRAKDFVAEVKALTDGRGVDEILDMVGGPYVARNLQALAVE
GRLVQIAFLQPSEVTIDLMPVMLKRLTFTGSTLRARSVALKSAIAEALKRDVWPLLEAGKIKPFVHATFPLEQAREAHAL
MESSAHLGKIMLETGR
>Mature_335_residues
PVVEIPSSMRQARFTGAGGPEVIAIETAPVPRPGPGQVLVEVVAAGVNRPDCLQRAGNYPPPPGATEIPGLEIAGRVVAL
GEGVDSLKIGDEVCALVISGGYAEYCVAEAPLCLPIPKPLSLLEAAGLPENYYTVYDNVFTRGRLKAGETILIHGGSSGI
GSTAIQLAHHAGARVIATAGSPEKCDFCLKLGADAAIDYRAKDFVAEVKALTDGRGVDEILDMVGGPYVARNLQALAVEG
RLVQIAFLQPSEVTIDLMPVMLKRLTFTGSTLRARSVALKSAIAEALKRDVWPLLEAGKIKPFVHATFPLEQAREAHALM
ESSAHLGKIMLETGR

Specific function: Catalyzes the synthesis of methyl phthioceranic acids and/or hydroxyphthioceranic acids that are the major acyl constituents of cell wall lipids [H]

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=329, Percent_Identity=37.6899696048632, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI22538444, Length=329, Percent_Identity=37.6899696048632, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI194239674, Length=328, Percent_Identity=32.0121951219512, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI13236495, Length=328, Percent_Identity=32.0121951219512, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI194239676, Length=326, Percent_Identity=30.0613496932515, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI18379349, Length=342, Percent_Identity=28.6549707602339, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI24308257, Length=346, Percent_Identity=26.5895953757225, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI41872631, Length=312, Percent_Identity=29.8076923076923, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI28557745, Length=321, Percent_Identity=30.5295950155763, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI47519420, Length=331, Percent_Identity=29.3051359516616, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI197927207, Length=190, Percent_Identity=30, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1790485, Length=323, Percent_Identity=31.8885448916409, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI226510941, Length=334, Percent_Identity=24.8502994011976, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507255, Length=329, Percent_Identity=31.9148936170213, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI212642053, Length=305, Percent_Identity=29.8360655737705, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988145, Length=301, Percent_Identity=23.5880398671096, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71987554, Length=366, Percent_Identity=27.3224043715847, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17536829, Length=198, Percent_Identity=30.8080808080808, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17562582, Length=308, Percent_Identity=27.9220779220779, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=336, Percent_Identity=29.1666666666667, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323961, Length=304, Percent_Identity=27.3026315789474, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6319621, Length=365, Percent_Identity=26.027397260274, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324486, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=360, Percent_Identity=24.4444444444444, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24581345, Length=319, Percent_Identity=29.7805642633229, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI221330659, Length=273, Percent_Identity=31.5018315018315, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920632, Length=275, Percent_Identity=31.2727272727273, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI45550423, Length=312, Percent_Identity=24.0384615384615, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001227
- InterPro:   IPR009081
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR000794
- InterPro:   IPR002198
- InterPro:   IPR011032
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016036
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020801
- InterPro:   IPR020807
- InterPro:   IPR020843
- InterPro:   IPR006162
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF08240 ADH_N; PF00106 adh_short; PF00107 ADH_zinc_N; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35387; Mature: 35256

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVEIPSSMRQARFTGAGGPEVIAIETAPVPRPGPGQVLVEVVAAGVNRPDCLQRAGNY
CCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCC
PPPPGATEIPGLEIAGRVVALGEGVDSLKIGDEVCALVISGGYAEYCVAEAPLCLPIPKP
CCCCCCCCCCCCHHCCEEEEECCCCCCHHHHHHHEEEEECCCCHHHHHHCCCEEECCCCC
LSLLEAAGLPENYYTVYDNVFTRGRLKAGETILIHGGSSGIGSTAIQLAHHAGARVIATA
HHHHHHCCCCCCCEEHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEC
GSPEKCDFCLKLGADAAIDYRAKDFVAEVKALTDGRGVDEILDMVGGPYVARNLQALAVE
CCCHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHCCEEEEEC
GRLVQIAFLQPSEVTIDLMPVMLKRLTFTGSTLRARSVALKSAIAEALKRDVWPLLEAGK
CEEEEEEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IKPFVHATFPLEQAREAHALMESSAHLGKIMLETGR
CCCEEEEECCHHHHHHHHHHHHCCHHHCEEEEECCC
>Mature Secondary Structure 
PVVEIPSSMRQARFTGAGGPEVIAIETAPVPRPGPGQVLVEVVAAGVNRPDCLQRAGNY
CCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCC
PPPPGATEIPGLEIAGRVVALGEGVDSLKIGDEVCALVISGGYAEYCVAEAPLCLPIPKP
CCCCCCCCCCCCHHCCEEEEECCCCCCHHHHHHHEEEEECCCCHHHHHHCCCEEECCCCC
LSLLEAAGLPENYYTVYDNVFTRGRLKAGETILIHGGSSGIGSTAIQLAHHAGARVIATA
HHHHHHCCCCCCCEEHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEC
GSPEKCDFCLKLGADAAIDYRAKDFVAEVKALTDGRGVDEILDMVGGPYVARNLQALAVE
CCCHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHCCEEEEEC
GRLVQIAFLQPSEVTIDLMPVMLKRLTFTGSTLRARSVALKSAIAEALKRDVWPLLEAGK
CEEEEEEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IKPFVHATFPLEQAREAHALMESSAHLGKIMLETGR
CCCEEEEECCHHHHHHHHHHHHCCHHHCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]