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The map label for this gene is garD [H]

Identifier: 154245548

GI number: 154245548

Start: 1813536

End: 1815077

Strand: Direct

Name: garD [H]

Synonym: Xaut_1602

Alternate gene names: 154245548

Gene position: 1813536-1815077 (Clockwise)

Preceding gene: 154245543

Following gene: 154245549

Centisome position: 34.16

GC content: 67.83

Gene sequence:

>1542_bases
ATGCTGACGACGTCCGCCGCATCACCCCTCATCCACCTGCATGCGTCGGACAATGTCGGGGTGGCGCGCGCGCACATTCC
GGCGGGAGCCGCCGTCATCCTCGATGGAATGGGCGTGACCGCGCTGGCCGAGATCCCGAGCGGCCACAAGATGGCCGTCG
CCCCCATCCCCGCGGGCTCGCGTGTGCTGAAGTACGGCCAGACCATCGGCGTCGCCACCGCCGACATCGCGCCCGGCGCC
CATGTCCACCTGCACAACCTCGCCATGCAGGAGAGCGATGTCGCTCACCACTTCGCCGAGGACGGCCGCGCCACCGAGCT
TTTGCCGCAGGCCGCGCGGCGCACCTTCGAGGGCTATGTGCGGCCCGACGGCCAGGTGGGCACGCGCAACTACATCGGCA
TCGTCACCTCGGTGAACTGCTCGGCCACGGTGGCTCGCGCCGTCGCCGATCATTTCAACCGCGGCGGGCTGGAGGGCTTT
GGTAATGTGGACGGCGTGGTCGCCCTCACCCACGGCACCGGCTGCGCCATCAACACCAAGACCGAGGGCTACGCCTATCT
CACCCGCGTGCTCGGCGGCTATGCCAAGAACCCGAATTTCGCCGGCATCCTGATGATCGGCCTCGGCTGCGAGACCAACC
AGATCGAGCCCATCGTTTCCCGCGAGGGGCTGGTGGAGGGCCGGCTGCTGCGCACCATGACCATCCAGGGTCTCGGCGGC
ACCCGCAAGACCATCGAGACCGCCAGCGCCATCATCCGCGAGATGCTGCCCATCGCGAATGCCGCCCAGCGCACCACGGT
GCCCCTCACCGGCCTGAAGGTGGCGCTGCAATGCGGCGGCTCGGACGGCTATTCGGGCATCTCGGCGAACCCGGCACTGG
GCTATGCCGCCGACCTGGTGGTGAAGCACGGCGGCACGGCGGTGCTCAGCGAAACGCCGGAAATATACGGCGCCGAGCAC
CTGCTCACCCGCCGCGCGGTGAGCCCCGAGGTGGCGCAGAAGCTGATGGACCGCATCGACTGGTGGCGCACCTACACCGA
GCGCAACGGCGCGGAGCTGAACAACAATCCCTCCCACGGCAACAAGGCCGGCGGGCTCACCACCATCCTGGAGAAGTCGC
TGGGCGCGGTGGCCAAGGGCGGCTCCATGCCGCTCGAGGCGGTCTATGAATATGCCGAGATCATCGATCGCACGGGCTTT
GTGTTCATGGACACGCCCGGCTACGATCCGGTGGCGGTGACCGGCCAGGTGGCGGGGGGCTGCAACGTCGTCGTCTTCAC
CACCGGCCGTGGCTCGGTCTCCGGCTTCAAGCCGGCGCCTTGCATCAAGGTCGCCACCAATACCCAGATGTACGAGCACA
TGCACGAGGACATGGACATCAACTGCGGCGGCATCGTCACCGGCGAGGACACCATCGCCGCCGCGGGCGAGCGCATCTTC
GAGATGATTCTTGCCGTGGCCTCGGGCGCGCATACGGCGAGCGAGGATTTCGGCTTCGGCGACAGCGAGTTCGTGCCATG
GCAGATCGGCGCCGTCACCTGA

Upstream 100 bases:

>100_bases
GCGTCCCCATAACACTGATATTTGTTATATTTTTTGTTTGACTAAACCATGTCTAACATGATTGATTGATGCACGCAATG
ATCATTTTTAGGGAAGCGCC

Downstream 100 bases:

>100_bases
GCGCGGCGCGGACCGCCGCGCCCGTTGTGGATCGCACGCCGGCTTATGCGGTCATCGCCGGCCGGCTGCGCCGCTCCATC
GCGGCGGGCGTGCTGCCGGA

Product: galactarate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MLTTSAASPLIHLHASDNVGVARAHIPAGAAVILDGMGVTALAEIPSGHKMAVAPIPAGSRVLKYGQTIGVATADIAPGA
HVHLHNLAMQESDVAHHFAEDGRATELLPQAARRTFEGYVRPDGQVGTRNYIGIVTSVNCSATVARAVADHFNRGGLEGF
GNVDGVVALTHGTGCAINTKTEGYAYLTRVLGGYAKNPNFAGILMIGLGCETNQIEPIVSREGLVEGRLLRTMTIQGLGG
TRKTIETASAIIREMLPIANAAQRTTVPLTGLKVALQCGGSDGYSGISANPALGYAADLVVKHGGTAVLSETPEIYGAEH
LLTRRAVSPEVAQKLMDRIDWWRTYTERNGAELNNNPSHGNKAGGLTTILEKSLGAVAKGGSMPLEAVYEYAEIIDRTGF
VFMDTPGYDPVAVTGQVAGGCNVVVFTTGRGSVSGFKPAPCIKVATNTQMYEHMHEDMDINCGGIVTGEDTIAAAGERIF
EMILAVASGAHTASEDFGFGDSEFVPWQIGAVT

Sequences:

>Translated_513_residues
MLTTSAASPLIHLHASDNVGVARAHIPAGAAVILDGMGVTALAEIPSGHKMAVAPIPAGSRVLKYGQTIGVATADIAPGA
HVHLHNLAMQESDVAHHFAEDGRATELLPQAARRTFEGYVRPDGQVGTRNYIGIVTSVNCSATVARAVADHFNRGGLEGF
GNVDGVVALTHGTGCAINTKTEGYAYLTRVLGGYAKNPNFAGILMIGLGCETNQIEPIVSREGLVEGRLLRTMTIQGLGG
TRKTIETASAIIREMLPIANAAQRTTVPLTGLKVALQCGGSDGYSGISANPALGYAADLVVKHGGTAVLSETPEIYGAEH
LLTRRAVSPEVAQKLMDRIDWWRTYTERNGAELNNNPSHGNKAGGLTTILEKSLGAVAKGGSMPLEAVYEYAEIIDRTGF
VFMDTPGYDPVAVTGQVAGGCNVVVFTTGRGSVSGFKPAPCIKVATNTQMYEHMHEDMDINCGGIVTGEDTIAAAGERIF
EMILAVASGAHTASEDFGFGDSEFVPWQIGAVT
>Mature_513_residues
MLTTSAASPLIHLHASDNVGVARAHIPAGAAVILDGMGVTALAEIPSGHKMAVAPIPAGSRVLKYGQTIGVATADIAPGA
HVHLHNLAMQESDVAHHFAEDGRATELLPQAARRTFEGYVRPDGQVGTRNYIGIVTSVNCSATVARAVADHFNRGGLEGF
GNVDGVVALTHGTGCAINTKTEGYAYLTRVLGGYAKNPNFAGILMIGLGCETNQIEPIVSREGLVEGRLLRTMTIQGLGG
TRKTIETASAIIREMLPIANAAQRTTVPLTGLKVALQCGGSDGYSGISANPALGYAADLVVKHGGTAVLSETPEIYGAEH
LLTRRAVSPEVAQKLMDRIDWWRTYTERNGAELNNNPSHGNKAGGLTTILEKSLGAVAKGGSMPLEAVYEYAEIIDRTGF
VFMDTPGYDPVAVTGQVAGGCNVVVFTTGRGSVSGFKPAPCIKVATNTQMYEHMHEDMDINCGGIVTGEDTIAAAGERIF
EMILAVASGAHTASEDFGFGDSEFVPWQIGAVT

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=498, Percent_Identity=38.1526104417671, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1789475, Length=497, Percent_Identity=35.2112676056338, Blast_Score=278, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 53618; Mature: 53618

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTSAASPLIHLHASDNVGVARAHIPAGAAVILDGMGVTALAEIPSGHKMAVAPIPAGS
CCCCCCCCCEEEEECCCCCCEEEEECCCCCEEEEECCCCEEEEECCCCCEEEEEECCCCH
RVLKYGQTIGVATADIAPGAHVHLHNLAMQESDVAHHFAEDGRATELLPQAARRTFEGYV
HHHHCCCEECEEEECCCCCCEEEEEHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
RPDGQVGTRNYIGIVTSVNCSATVARAVADHFNRGGLEGFGNVDGVVALTHGTGCAINTK
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECC
TEGYAYLTRVLGGYAKNPNFAGILMIGLGCETNQIEPIVSREGLVEGRLLRTMTIQGLGG
CCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCCCCEEEEEEEEECCCC
TRKTIETASAIIREMLPIANAAQRTTVPLTGLKVALQCGGSDGYSGISANPALGYAADLV
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHEEEE
VKHGGTAVLSETPEIYGAEHLLTRRAVSPEVAQKLMDRIDWWRTYTERNGAELNNNPSHG
EECCCEEEECCCCCHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NKAGGLTTILEKSLGAVAKGGSMPLEAVYEYAEIIDRTGFVFMDTPGYDPVAVTGQVAGG
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCC
CNVVVFTTGRGSVSGFKPAPCIKVATNTQMYEHMHEDMDINCGGIVTGEDTIAAAGERIF
CEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHH
EMILAVASGAHTASEDFGFGDSEFVPWQIGAVT
HHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCC
>Mature Secondary Structure
MLTTSAASPLIHLHASDNVGVARAHIPAGAAVILDGMGVTALAEIPSGHKMAVAPIPAGS
CCCCCCCCCEEEEECCCCCCEEEEECCCCCEEEEECCCCEEEEECCCCCEEEEEECCCCH
RVLKYGQTIGVATADIAPGAHVHLHNLAMQESDVAHHFAEDGRATELLPQAARRTFEGYV
HHHHCCCEECEEEECCCCCCEEEEEHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
RPDGQVGTRNYIGIVTSVNCSATVARAVADHFNRGGLEGFGNVDGVVALTHGTGCAINTK
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECC
TEGYAYLTRVLGGYAKNPNFAGILMIGLGCETNQIEPIVSREGLVEGRLLRTMTIQGLGG
CCCHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCHHHCCCCCCCEEEEEEEEECCCC
TRKTIETASAIIREMLPIANAAQRTTVPLTGLKVALQCGGSDGYSGISANPALGYAADLV
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHEEEE
VKHGGTAVLSETPEIYGAEHLLTRRAVSPEVAQKLMDRIDWWRTYTERNGAELNNNPSHG
EECCCEEEECCCCCHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
NKAGGLTTILEKSLGAVAKGGSMPLEAVYEYAEIIDRTGFVFMDTPGYDPVAVTGQVAGG
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCC
CNVVVFTTGRGSVSGFKPAPCIKVATNTQMYEHMHEDMDINCGGIVTGEDTIAAAGERIF
CEEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHH
EMILAVASGAHTASEDFGFGDSEFVPWQIGAVT
HHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]