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The map label for this gene is dmlR [H]

Identifier: 154245537

GI number: 154245537

Start: 1802172

End: 1803113

Strand: Reverse

Name: dmlR [H]

Synonym: Xaut_1591

Alternate gene names: 154245537

Gene position: 1803113-1802172 (Counterclockwise)

Preceding gene: 154245539

Following gene: 154245533

Centisome position: 33.96

GC content: 72.51

Gene sequence:

>942_bases
GTGCTGGACGACCTGAATGAGCTGAAGACGTTCCGGGCGATCCTGGCCGAGGGTAGCCTGTCGGCCGCCGCGCGGCGGCT
CGGCATCACCCTCGCGGTGGTGAGCAAGCGGCTGGCGACGCTGGAGGATCGGGCCGGCGTACGCCTCATCCAGCGCACCA
CCCGCGCGCTCAGCCCCACCGAGGAAGGCGCGCGCCTGCTGATCGATGTGGAGCGTGCGCTGGACGCCATCGAGGCCGGC
GAGGAGCAGCTGGCGAGCGGGCGGGTCGAACCCTCGGGCACCCTGCGGGTCAGCGCGCCCATCGCCTTCGGCCGCCGTTG
CGTGGCGCCGGTTCTCGCCCAACTGGCCGCGCGCCATGCCCGACTCGGGGTCGCGCTGGAACTGGACGACCGGGTGGTGG
ACCTGGTGGGAGAGGGGTTCGACGTGGCCATCCGCATCGGCGCGCCCGCCGACAGTTCGGCCATGATGCGCAAGCTCGCC
GACAACCGGCGCATCCTGGTCGCCGCTCCGGCCTATCTCGACCGGGCGGGCCGGCCGGTGACACCGGAGGAGGCCGCCGG
CCATGCGTTCCTGCGCTACGGCCGCGGCAGCGACCCGTGGCGGCTGCGGGGACCGGACGGAGCAACGGCCAGCCTTGGCG
CGGCGGCGCGGTTGCGTGTCGACGATGGCGACGTGGTGCACGACTGGGCGCTTGCCGGCCTCGGCATCATGCTCAAGTCC
GAGGTGGATGTGGCGCAGGACCTTGCCACCGGTCGGCTCACCCGCGTGCTGCCCGGTTGGGATGGCGGAGATGCGCCCAT
TCTCGCGCTTTATCCCAGTGCCCGGCATGTGCCGCTGAAGACGCGGGTGCTGCTCGACGCGCTCGCCGCTCACCTCGCCA
CCCTCATGACCGGCGAAAGCTGCCGGCCCGCGGCACCCCTGCCGGCGCTTGACGTGCTTTAA

Upstream 100 bases:

>100_bases
GAAAGTCCAATTCGCCATGAGGGCAATGTGCGCTCTTGTTCTGAAGCTGATAAGCGAGCATGTATACGAGGCATTCTCGT
ATTCAAGTTGAGAATATGCG

Downstream 100 bases:

>100_bases
CTAGCCGTACCGCCGGCTCGCCTCTATGGCGAGGCCGGTGCCCACCGAGCCAAAGGTGTCGCCCTCGATCACCCGGGCCT
GGGGCACGCAGGTGCAGATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLDDLNELKTFRAILAEGSLSAAARRLGITLAVVSKRLATLEDRAGVRLIQRTTRALSPTEEGARLLIDVERALDAIEAG
EEQLASGRVEPSGTLRVSAPIAFGRRCVAPVLAQLAARHARLGVALELDDRVVDLVGEGFDVAIRIGAPADSSAMMRKLA
DNRRILVAAPAYLDRAGRPVTPEEAAGHAFLRYGRGSDPWRLRGPDGATASLGAAARLRVDDGDVVHDWALAGLGIMLKS
EVDVAQDLATGRLTRVLPGWDGGDAPILALYPSARHVPLKTRVLLDALAAHLATLMTGESCRPAAPLPALDVL

Sequences:

>Translated_313_residues
MLDDLNELKTFRAILAEGSLSAAARRLGITLAVVSKRLATLEDRAGVRLIQRTTRALSPTEEGARLLIDVERALDAIEAG
EEQLASGRVEPSGTLRVSAPIAFGRRCVAPVLAQLAARHARLGVALELDDRVVDLVGEGFDVAIRIGAPADSSAMMRKLA
DNRRILVAAPAYLDRAGRPVTPEEAAGHAFLRYGRGSDPWRLRGPDGATASLGAAARLRVDDGDVVHDWALAGLGIMLKS
EVDVAQDLATGRLTRVLPGWDGGDAPILALYPSARHVPLKTRVLLDALAAHLATLMTGESCRPAAPLPALDVL
>Mature_313_residues
MLDDLNELKTFRAILAEGSLSAAARRLGITLAVVSKRLATLEDRAGVRLIQRTTRALSPTEEGARLLIDVERALDAIEAG
EEQLASGRVEPSGTLRVSAPIAFGRRCVAPVLAQLAARHARLGVALELDDRVVDLVGEGFDVAIRIGAPADSSAMMRKLA
DNRRILVAAPAYLDRAGRPVTPEEAAGHAFLRYGRGSDPWRLRGPDGATASLGAAARLRVDDGDVVHDWALAGLGIMLKS
EVDVAQDLATGRLTRVLPGWDGGDAPILALYPSARHVPLKTRVLLDALAAHLATLMTGESCRPAAPLPALDVL

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=35.6164383561644, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=33.6769759450172, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.3515358361775, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=30.5084745762712, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787128, Length=303, Percent_Identity=27.7227722772277, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=26.6211604095563, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789173, Length=256, Percent_Identity=27.34375, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=257, Percent_Identity=24.124513618677, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=250, Percent_Identity=24, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33146; Mature: 33146

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDLNELKTFRAILAEGSLSAAARRLGITLAVVSKRLATLEDRAGVRLIQRTTRALSPT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEGARLLIDVERALDAIEAGEEQLASGRVEPSGTLRVSAPIAFGRRCVAPVLAQLAARHA
CCCCEEEEEHHHHHHHHHCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
RLGVALELDDRVVDLVGEGFDVAIRIGAPADSSAMMRKLADNRRILVAAPAYLDRAGRPV
HCCEEEEECHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHCCCCCC
TPEEAAGHAFLRYGRGSDPWRLRGPDGATASLGAAARLRVDDGDVVHDWALAGLGIMLKS
CHHHHCCCCEEEECCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHEEEC
EVDVAQDLATGRLTRVLPGWDGGDAPILALYPSARHVPLKTRVLLDALAAHLATLMTGES
HHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
CRPAAPLPALDVL
CCCCCCCCHHCCC
>Mature Secondary Structure
MLDDLNELKTFRAILAEGSLSAAARRLGITLAVVSKRLATLEDRAGVRLIQRTTRALSPT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEGARLLIDVERALDAIEAGEEQLASGRVEPSGTLRVSAPIAFGRRCVAPVLAQLAARHA
CCCCEEEEEHHHHHHHHHCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
RLGVALELDDRVVDLVGEGFDVAIRIGAPADSSAMMRKLADNRRILVAAPAYLDRAGRPV
HCCEEEEECHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHCCCCCC
TPEEAAGHAFLRYGRGSDPWRLRGPDGATASLGAAARLRVDDGDVVHDWALAGLGIMLKS
CHHHHCCCCEEEECCCCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHEEEC
EVDVAQDLATGRLTRVLPGWDGGDAPILALYPSARHVPLKTRVLLDALAAHLATLMTGES
HHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
CRPAAPLPALDVL
CCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]