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The map label for this gene is tktB [C]

Identifier: 154245487

GI number: 154245487

Start: 1750311

End: 1751159

Strand: Reverse

Name: tktB [C]

Synonym: Xaut_1540

Alternate gene names: 154245487

Gene position: 1751159-1750311 (Counterclockwise)

Preceding gene: 154245488

Following gene: 154245486

Centisome position: 32.99

GC content: 66.55

Gene sequence:

>849_bases
ATGACCATCGCAACTTCCGGCCACAACCGGCAGCTTGCCGAACGCGCGCACGCCATCCGGCGCCATGCGCTGCGCATGGG
CGAGGTGCAGGGCCAGGGCTACATCGCGCAGGCGCTCGGCATCTCCGACGTTCTGGCGGCAGCCTATTTCCATGCCATGA
CCTATCGGGCCGAAGATCCGGACTGGGAAGGACGCGACCGCTTCCTGCTGTCCAACGGGCACTATGCCATCGCGCTCTAT
GCGGCGCTGATCGAGGCCGGCATCGTCCCTGAGGACGAGCTTGAGACCTACGGCTTCGACGACAGCCGGCTGCCCATGTC
GGGCATGGCCAGCTACACGCCGGGCATGGAGATGTCGGGCGGCTCGCTCGGCCTTGGCCTCGCCATCGCTGTGGGCCGCT
GCCTCGGCCTCAAGCGCAAAGGCTCCGACCGCCACGTCTACACCCTGTTCTCCGACGGTGAGTTGGACGAGGGCTCGGTG
TGGGAGGCCATCATGTCGGCCGCCCACTTCCAGCTCGACAACCTCATCGCCGTGGTGGACGTCAATAACCAGCAGGCCGA
CGGGCCGTCCACCTCGGTGCTCGGCTTCGAGCCGCTGAAGGAGAAGCTGGAAGCCTTCGGCTGGTTCGTCTGCCGCGTGG
ACGGCAACGACCTCGAAGCGGTGCGCAAGGCCTTCGATGCGTGCAAGGCGCAATCGGGCGGCGGCAAGCCCAGCATGATC
ATCGCCGACACGCTGATGGGCAAGGGCGTGCCGTTCCTTGAGGCGCGCGAGAAGAACCACTTCATCCGCGTCGACGCCCA
CGAGTGGCAACTGGCGCTGGCCGCACTCGACGAGGGGAAGCCGTCATGA

Upstream 100 bases:

>100_bases
TGTCCGCCTACGTCACCGGCGCCGTGATCGACGTCAACGGCGGCATGCTCATCCACTGAGCGCTCCGGCGCTGCGGCGCC
CAGCCTTCGAGGAAAACCAG

Downstream 100 bases:

>100_bases
ACCACGTAACCCGCAAGGCCGCCCCTGCCGCCCCCCCTGCCGCCGCTGCTGCCGGCAAGCCCCGGCTGACCACCTCCGCG
ATGATCGCCTCAATCGCCGC

Product: transketolase domain-containing protein

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTIATSGHNRQLAERAHAIRRHALRMGEVQGQGYIAQALGISDVLAAAYFHAMTYRAEDPDWEGRDRFLLSNGHYAIALY
AALIEAGIVPEDELETYGFDDSRLPMSGMASYTPGMEMSGGSLGLGLAIAVGRCLGLKRKGSDRHVYTLFSDGELDEGSV
WEAIMSAAHFQLDNLIAVVDVNNQQADGPSTSVLGFEPLKEKLEAFGWFVCRVDGNDLEAVRKAFDACKAQSGGGKPSMI
IADTLMGKGVPFLEAREKNHFIRVDAHEWQLALAALDEGKPS

Sequences:

>Translated_282_residues
MTIATSGHNRQLAERAHAIRRHALRMGEVQGQGYIAQALGISDVLAAAYFHAMTYRAEDPDWEGRDRFLLSNGHYAIALY
AALIEAGIVPEDELETYGFDDSRLPMSGMASYTPGMEMSGGSLGLGLAIAVGRCLGLKRKGSDRHVYTLFSDGELDEGSV
WEAIMSAAHFQLDNLIAVVDVNNQQADGPSTSVLGFEPLKEKLEAFGWFVCRVDGNDLEAVRKAFDACKAQSGGGKPSMI
IADTLMGKGVPFLEAREKNHFIRVDAHEWQLALAALDEGKPS
>Mature_281_residues
TIATSGHNRQLAERAHAIRRHALRMGEVQGQGYIAQALGISDVLAAAYFHAMTYRAEDPDWEGRDRFLLSNGHYAIALYA
ALIEAGIVPEDELETYGFDDSRLPMSGMASYTPGMEMSGGSLGLGLAIAVGRCLGLKRKGSDRHVYTLFSDGELDEGSVW
EAIMSAAHFQLDNLIAVVDVNNQQADGPSTSVLGFEPLKEKLEAFGWFVCRVDGNDLEAVRKAFDACKAQSGGGKPSMII
ADTLMGKGVPFLEAREKNHFIRVDAHEWQLALAALDEGKPS

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=254, Percent_Identity=38.1889763779528, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI205277463, Length=254, Percent_Identity=36.6141732283465, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI4507521, Length=254, Percent_Identity=36.6141732283465, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI225637459, Length=257, Percent_Identity=29.5719844357977, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI225637461, Length=221, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI225637463, Length=219, Percent_Identity=31.0502283105023, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788808, Length=259, Percent_Identity=34.7490347490347, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994911, Length=254, Percent_Identity=32.6771653543307, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17539652, Length=264, Percent_Identity=31.8181818181818, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325331, Length=246, Percent_Identity=33.739837398374, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319593, Length=231, Percent_Identity=31.6017316017316, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI45551847, Length=255, Percent_Identity=32.156862745098, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45550715, Length=255, Percent_Identity=32.156862745098, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24666278, Length=255, Percent_Identity=30.9803921568627, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24645119, Length=220, Percent_Identity=34.5454545454545, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 30605; Mature: 30474

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIATSGHNRQLAERAHAIRRHALRMGEVQGQGYIAQALGISDVLAAAYFHAMTYRAEDP
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCHHHHHHHHHHHHHHCCCCCC
DWEGRDRFLLSNGHYAIALYAALIEAGIVPEDELETYGFDDSRLPMSGMASYTPGMEMSG
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHCCCCCCCCC
GSLGLGLAIAVGRCLGLKRKGSDRHVYTLFSDGELDEGSVWEAIMSAAHFQLDNLIAVVD
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEE
VNNQQADGPSTSVLGFEPLKEKLEAFGWFVCRVDGNDLEAVRKAFDACKAQSGGGKPSMI
CCCCCCCCCCCEEECCHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEE
IADTLMGKGVPFLEAREKNHFIRVDAHEWQLALAALDEGKPS
EEEHHHCCCCCCEEECCCCCEEEEECHHHEEEEEECCCCCCC
>Mature Secondary Structure 
TIATSGHNRQLAERAHAIRRHALRMGEVQGQGYIAQALGISDVLAAAYFHAMTYRAEDP
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCHHHHHHHHHHHHHHCCCCCC
DWEGRDRFLLSNGHYAIALYAALIEAGIVPEDELETYGFDDSRLPMSGMASYTPGMEMSG
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHCCCCCCCCC
GSLGLGLAIAVGRCLGLKRKGSDRHVYTLFSDGELDEGSVWEAIMSAAHFQLDNLIAVVD
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEE
VNNQQADGPSTSVLGFEPLKEKLEAFGWFVCRVDGNDLEAVRKAFDACKAQSGGGKPSMI
CCCCCCCCCCCEEECCHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEE
IADTLMGKGVPFLEAREKNHFIRVDAHEWQLALAALDEGKPS
EEEHHHCCCCCCEEECCCCCEEEEECHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]