Click here to switch to the map view.

The map label for this gene is hprA [H]

Identifier: 154245481

GI number: 154245481

Start: 1742036

End: 1743025

Strand: Reverse

Name: hprA [H]

Synonym: Xaut_1534

Alternate gene names: 154245481

Gene position: 1743025-1742036 (Counterclockwise)

Preceding gene: 154245482

Following gene: 154245480

Centisome position: 32.83

GC content: 71.92

Gene sequence:

>990_bases
GTGAACGCGCCCTTCCCGCCCCTGGGCCGCGTCCCGCGCATCGTCTTCCTCGATCGCGAGACGCTGGCGCCGGCCGTGAC
GCTCGCGCGCCCGGCCTTCGCCCACGACTGGGTGGAATTCGACCGCACCGCGCCGGCCGAACTGATGGAGCGGGCCCGCG
ATGCGGACATCCTCATCCTCAACAAGGTGCGGCTCGACGCCGAAGCCATCGCCGGGCTGCCGCGCCTCGCTTTGATCGCG
GTGGCGGCCACCGGCACCGACTGCGTGGACAAGGCCGCCTGCGCTGCAGCCGGCATCGCGGTCAGCAACGTGCGCGGCTA
TGCCAAGGCAAGCGTGCCGGAGCACACCTTCGCCCTCATCCTCGCCCTGGCGCGGGCCATCGGCCCCTATGCGGCGGACG
TGAAGGCCGGTGCATGGCAGGCGGCGGGGCAATTCTGCTTCCACACCCATCCCATCATCGATTTGCACGGCCGGCGCCTC
GGCATCTTCGGCTCGGGGGATCTCGGCCAGCAGGTGGCGGCGCTTGGCCGCGCGTTCGGCATGGAGCCGGTGTTCGCCGG
CCGCAAGGGCGCGGGCGAGGCCCGCCCCGGCTACATGCCGTTCACCGAGGTGATCGAGACCGCGGACGTGCTCACCCTCC
ACTGCCCGCTGACGCTCGAGACGCGGGGATTGATCGGCGCTGAGGAGTTCGCCCGCATGGGCCGGCGGCCCCTCCTCGTG
AACACCGCCCGTGGTGGCCTTATTGACGAGGAGGCGCTGATCGCGGCGCTCGACGCCGGCCAGGTGGCGGGCGCTGGCAT
CGACGTGGCCATGCCCGAGCCGCCCCCCGCCGACAGCGCGGTGATGCGCCTCGCCCACCATCCGAAAGCCATCGTGACGC
CCCATGTGGCCTGGGCCTCCATGGAAGCGCAGCAGGCGCTGGCGGACCAGCTCATCGCCAATATCGAGGCCTTCGCCGCC
GGCCGCCCGGTGAACGTGGCAACGCCGTAG

Upstream 100 bases:

>100_bases
TGGGGATGAACAGCTTCGGTGCCTCGGGACCGGCCGACGAGTTGTTCCGCCATTTCGGCATCACCCCCGCCGCCGTGGGG
GATGCGGCGCGGGCGCGGCT

Downstream 100 bases:

>100_bases
ATCGGAGGAAACGCCCTTCCCGGCGGTCGGGCGCTGCATTTTCCCAATGGACCATGAGCTTTTCCCATGGTCCATTGGTC
AAGCCCGCGCGGCGCCTGAG

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNAPFPPLGRVPRIVFLDRETLAPAVTLARPAFAHDWVEFDRTAPAELMERARDADILILNKVRLDAEAIAGLPRLALIA
VAATGTDCVDKAACAAAGIAVSNVRGYAKASVPEHTFALILALARAIGPYAADVKAGAWQAAGQFCFHTHPIIDLHGRRL
GIFGSGDLGQQVAALGRAFGMEPVFAGRKGAGEARPGYMPFTEVIETADVLTLHCPLTLETRGLIGAEEFARMGRRPLLV
NTARGGLIDEEALIAALDAGQVAGAGIDVAMPEPPPADSAVMRLAHHPKAIVTPHVAWASMEAQQALADQLIANIEAFAA
GRPVNVATP

Sequences:

>Translated_329_residues
MNAPFPPLGRVPRIVFLDRETLAPAVTLARPAFAHDWVEFDRTAPAELMERARDADILILNKVRLDAEAIAGLPRLALIA
VAATGTDCVDKAACAAAGIAVSNVRGYAKASVPEHTFALILALARAIGPYAADVKAGAWQAAGQFCFHTHPIIDLHGRRL
GIFGSGDLGQQVAALGRAFGMEPVFAGRKGAGEARPGYMPFTEVIETADVLTLHCPLTLETRGLIGAEEFARMGRRPLLV
NTARGGLIDEEALIAALDAGQVAGAGIDVAMPEPPPADSAVMRLAHHPKAIVTPHVAWASMEAQQALADQLIANIEAFAA
GRPVNVATP
>Mature_329_residues
MNAPFPPLGRVPRIVFLDRETLAPAVTLARPAFAHDWVEFDRTAPAELMERARDADILILNKVRLDAEAIAGLPRLALIA
VAATGTDCVDKAACAAAGIAVSNVRGYAKASVPEHTFALILALARAIGPYAADVKAGAWQAAGQFCFHTHPIIDLHGRRL
GIFGSGDLGQQVAALGRAFGMEPVFAGRKGAGEARPGYMPFTEVIETADVLTLHCPLTLETRGLIGAEEFARMGRRPLLV
NTARGGLIDEEALIAALDAGQVAGAGIDVAMPEPPPADSAVMRLAHHPKAIVTPHVAWASMEAQQALADQLIANIEAFAA
GRPVNVATP

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=293, Percent_Identity=31.3993174061433, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI61743967, Length=292, Percent_Identity=29.1095890410959, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4557497, Length=292, Percent_Identity=29.1095890410959, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI145580578, Length=336, Percent_Identity=28.2738095238095, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI4557499, Length=336, Percent_Identity=28.2738095238095, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI145580575, Length=292, Percent_Identity=29.1095890410959, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI6912396, Length=321, Percent_Identity=25.8566978193146, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87082289, Length=282, Percent_Identity=30.4964539007092, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789279, Length=304, Percent_Identity=29.9342105263158, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787645, Length=257, Percent_Identity=26.4591439688716, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081824, Length=192, Percent_Identity=29.6875, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788660, Length=271, Percent_Identity=26.5682656826568, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=270, Percent_Identity=31.4814814814815, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=293, Percent_Identity=29.0102389078498, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=285, Percent_Identity=30.5263157894737, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=285, Percent_Identity=30.5263157894737, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324055, Length=264, Percent_Identity=30.3030303030303, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324964, Length=299, Percent_Identity=28.4280936454849, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324980, Length=144, Percent_Identity=31.25, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921140, Length=280, Percent_Identity=34.2857142857143, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28571528, Length=302, Percent_Identity=29.1390728476821, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24646446, Length=272, Percent_Identity=31.9852941176471, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24646448, Length=272, Percent_Identity=31.9852941176471, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24646452, Length=272, Percent_Identity=31.9852941176471, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24646450, Length=272, Percent_Identity=31.9852941176471, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI62472511, Length=272, Percent_Identity=31.9852941176471, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585516, Length=254, Percent_Identity=25.1968503937008, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28574286, Length=292, Percent_Identity=26.7123287671233, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585514, Length=289, Percent_Identity=24.9134948096886, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI28574282, Length=289, Percent_Identity=24.9134948096886, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI28574284, Length=289, Percent_Identity=24.9134948096886, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45552429, Length=289, Percent_Identity=24.9134948096886, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45551003, Length=289, Percent_Identity=24.9134948096886, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34537; Mature: 34537

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPFPPLGRVPRIVFLDRETLAPAVTLARPAFAHDWVEFDRTAPAELMERARDADILIL
CCCCCCCCCCCCEEEEECCHHHCCHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEE
NKVRLDAEAIAGLPRLALIAVAATGTDCVDKAACAAAGIAVSNVRGYAKASVPEHTFALI
EEEEECHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
LALARAIGPYAADVKAGAWQAAGQFCFHTHPIIDLHGRRLGIFGSGDLGQQVAALGRAFG
HHHHHHHCCCHHCCCCCCHHHHCCCEEECCCEEEECCCEEEEEECCCHHHHHHHHHHHHC
MEPVFAGRKGAGEARPGYMPFTEVIETADVLTLHCPLTLETRGLIGAEEFARMGRRPLLV
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCEEEECCCCCCHHHHHHCCCCCEEE
NTARGGLIDEEALIAALDAGQVAGAGIDVAMPEPPPADSAVMRLAHHPKAIVTPHVAWAS
ECCCCCCCCHHHHHEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEEECCCHHHHH
MEAQQALADQLIANIEAFAAGRPVNVATP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNAPFPPLGRVPRIVFLDRETLAPAVTLARPAFAHDWVEFDRTAPAELMERARDADILIL
CCCCCCCCCCCCEEEEECCHHHCCHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEE
NKVRLDAEAIAGLPRLALIAVAATGTDCVDKAACAAAGIAVSNVRGYAKASVPEHTFALI
EEEEECHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
LALARAIGPYAADVKAGAWQAAGQFCFHTHPIIDLHGRRLGIFGSGDLGQQVAALGRAFG
HHHHHHHCCCHHCCCCCCHHHHCCCEEECCCEEEECCCEEEEEECCCHHHHHHHHHHHHC
MEPVFAGRKGAGEARPGYMPFTEVIETADVLTLHCPLTLETRGLIGAEEFARMGRRPLLV
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCEEEECCCCCCHHHHHHCCCCCEEE
NTARGGLIDEEALIAALDAGQVAGAGIDVAMPEPPPADSAVMRLAHHPKAIVTPHVAWAS
ECCCCCCCCHHHHHEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEEECCCHHHHH
MEAQQALADQLIANIEAFAAGRPVNVATP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]