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The map label for this gene is bigR [H]

Identifier: 154245474

GI number: 154245474

Start: 1734971

End: 1735345

Strand: Direct

Name: bigR [H]

Synonym: Xaut_1527

Alternate gene names: 154245474

Gene position: 1734971-1735345 (Clockwise)

Preceding gene: 154245473

Following gene: 154245475

Centisome position: 32.68

GC content: 62.93

Gene sequence:

>375_bases
ATGCCACCCATCGTGTCCACGCGGATCGAGGACGAACTGCGCGAAGGCCCGGAGATTTCGCCCGAACTCGACCAGCTTAT
GCGCAATGCGCGCAAGGCGAGCGATTTCCTGAAAGCGCTCTCCCACGAGAACCGGCTCTTGCTGCTGTGCCTGCTGGCCG
AGCGCGAGCGGTCGGTGACCGAGCTGGAGAATATCCTGTCCCTGCGCCAGCCCACGGTGTCCCAGCAACTGGCACGGCTG
CGGCTGGACGATCTCGTCACCACGCGCCGCGACGGCAAGACCATCTATTACAGCCTCGCCAACGACGACGTGCGTCGGGT
CATCTCGGTCATCTACGACATGTATTGCGGGTCGGAACGCGCCGACAAGAGTTAA

Upstream 100 bases:

>100_bases
TGAAGCCTTCTGGGGTCTGCTGGGCGCGCTGACGCAACAGGGCGCGCCTAATCGGCCGTGAGGCCGGAGCGCATCGTCGA
TTTGTGAGAGGTTCGAGAGC

Downstream 100 bases:

>100_bases
GACAAAACAGCGCCGGGCCGGCGACGGGGCGACACAAGTCCCGCGCCTGCCGTCTACGGGAGGAGGCGGCGCATGGATAT
TTCAAGCCCCTGGTGGACCG

Product: regulatory protein ArsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 123

Protein sequence:

>124_residues
MPPIVSTRIEDELREGPEISPELDQLMRNARKASDFLKALSHENRLLLLCLLAERERSVTELENILSLRQPTVSQQLARL
RLDDLVTTRRDGKTIYYSLANDDVRRVISVIYDMYCGSERADKS

Sequences:

>Translated_124_residues
MPPIVSTRIEDELREGPEISPELDQLMRNARKASDFLKALSHENRLLLLCLLAERERSVTELENILSLRQPTVSQQLARL
RLDDLVTTRRDGKTIYYSLANDDVRRVISVIYDMYCGSERADKS
>Mature_123_residues
PPIVSTRIEDELREGPEISPELDQLMRNARKASDFLKALSHENRLLLLCLLAERERSVTELENILSLRQPTVSQQLARLR
LDDLVTTRRDGKTIYYSLANDDVRRVISVIYDMYCGSERADKS

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=96, Percent_Identity=40.625, Blast_Score=76, Evalue=7e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 14306; Mature: 14174

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPIVSTRIEDELREGPEISPELDQLMRNARKASDFLKALSHENRLLLLCLLAERERSVT
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHH
ELENILSLRQPTVSQQLARLRLDDLVTTRRDGKTIYYSLANDDVRRVISVIYDMYCGSER
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCC
ADKS
CCCC
>Mature Secondary Structure 
PPIVSTRIEDELREGPEISPELDQLMRNARKASDFLKALSHENRLLLLCLLAERERSVT
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHH
ELENILSLRQPTVSQQLARLRLDDLVTTRRDGKTIYYSLANDDVRRVISVIYDMYCGSER
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCC
ADKS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]