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The map label for this gene is dhfrIII [H]

Identifier: 154245367

GI number: 154245367

Start: 1623989

End: 1624525

Strand: Direct

Name: dhfrIII [H]

Synonym: Xaut_1420

Alternate gene names: 154245367

Gene position: 1623989-1624525 (Clockwise)

Preceding gene: 154245366

Following gene: 154245370

Centisome position: 30.59

GC content: 70.2

Gene sequence:

>537_bases
ATGACCCCCGGCCAGCCGCTCCGCCATCCTCTGGCCCTCGTGGTCGCGGTGTCGCAGAACGGGGTGATCGGCACCGACGG
CGGTCTGCCGTGGCGCCTGCCCTCGGACCTCAAGCGATTTCGCGCGCTCACCTGGGGCAAGCCGCTCCTCATGGGCCGGC
GCACCTACGAGTCCATCGGCCGTCCGCTGCCGGGGCGCACGTCCGTGGTGGTCAGCACCGATCCCGCCTTCACGGTGCCG
GAAGGAGTGCTGAAGGGGGCAAGCCTGGAGGACGGACTGCGCCTCGCCGATGAGGCGGCGGACGCCATGGGCGCGGATGA
GATCATGGTCATCGGCGGCGCCCGCCTGTTCCATGACACGCTGCCACTCGCCCGCACGCTGCATCTCACGCAGGTCCATG
CGGCACCTGATGGCGATGTCTTCTTCCCTGCGTTCGATGAGGCCCAATGGCGCGAGATCGCGCGGGACGGCCCGATCCAG
GGCGAGAAGGACGAAGCCGCCTTCACCGCTCTGACGCTGGTGCGCGCCGGCGGCTGA

Upstream 100 bases:

>100_bases
GAGGCTTTTTCCACCTGCCACGGCGCCCACCTCAGCGGCCTTGCCGCCGCCGATGCGGCCCTCGCCGCCCTGAACAGCGA
CCGATGATGGAGATCGCCTC

Downstream 100 bases:

>100_bases
AGCCGGCCTTCGTTTTCGACCGACGCTGAAGGAATACGCGAGGCTCGGCTGGTGGCGTCCGGACGCGTCCGGCCGAGCCT
CGCGATTTTCGGTGCTTCTA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MTPGQPLRHPLALVVAVSQNGVIGTDGGLPWRLPSDLKRFRALTWGKPLLMGRRTYESIGRPLPGRTSVVVSTDPAFTVP
EGVLKGASLEDGLRLADEAADAMGADEIMVIGGARLFHDTLPLARTLHLTQVHAAPDGDVFFPAFDEAQWREIARDGPIQ
GEKDEAAFTALTLVRAGG

Sequences:

>Translated_178_residues
MTPGQPLRHPLALVVAVSQNGVIGTDGGLPWRLPSDLKRFRALTWGKPLLMGRRTYESIGRPLPGRTSVVVSTDPAFTVP
EGVLKGASLEDGLRLADEAADAMGADEIMVIGGARLFHDTLPLARTLHLTQVHAAPDGDVFFPAFDEAQWREIARDGPIQ
GEKDEAAFTALTLVRAGG
>Mature_177_residues
TPGQPLRHPLALVVAVSQNGVIGTDGGLPWRLPSDLKRFRALTWGKPLLMGRRTYESIGRPLPGRTSVVVSTDPAFTVPE
GVLKGASLEDGLRLADEAADAMGADEIMVIGGARLFHDTLPLARTLHLTQVHAAPDGDVFFPAFDEAQWREIARDGPIQG
EKDEAAFTALTLVRAGG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=155, Percent_Identity=31.6129032258064, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1786233, Length=144, Percent_Identity=39.5833333333333, Blast_Score=110, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17505899, Length=168, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116008026, Length=160, Percent_Identity=33.125, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647458, Length=160, Percent_Identity=33.125, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19029; Mature: 18898

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGQPLRHPLALVVAVSQNGVIGTDGGLPWRLPSDLKRFRALTWGKPLLMGRRTYESIG
CCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHHHCCHHHHHHC
RPLPGRTSVVVSTDPAFTVPEGVLKGASLEDGLRLADEAADAMGADEIMVIGGARLFHDT
CCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHH
LPLARTLHLTQVHAAPDGDVFFPAFDEAQWREIARDGPIQGEKDEAAFTALTLVRAGG
HHHHHHEEEEEEECCCCCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHEEEECCC
>Mature Secondary Structure 
TPGQPLRHPLALVVAVSQNGVIGTDGGLPWRLPSDLKRFRALTWGKPLLMGRRTYESIG
CCCCCCCCCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHHHCCHHHHHHC
RPLPGRTSVVVSTDPAFTVPEGVLKGASLEDGLRLADEAADAMGADEIMVIGGARLFHDT
CCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHH
LPLARTLHLTQVHAAPDGDVFFPAFDEAQWREIARDGPIQGEKDEAAFTALTLVRAGG
HHHHHHEEEEEEECCCCCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]