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The map label for this gene is livM [H]

Identifier: 154245301

GI number: 154245301

Start: 1556007

End: 1556981

Strand: Direct

Name: livM [H]

Synonym: Xaut_1354

Alternate gene names: 154245301

Gene position: 1556007-1556981 (Clockwise)

Preceding gene: 154245300

Following gene: 154245302

Centisome position: 29.31

GC content: 67.59

Gene sequence:

>975_bases
ATGGCCGTGCGCTGGATCCTCCTCGCTCTCCTTCTCGCGGTGCTGGCGGCCGTGCCGGCCTTTGCCGCCTCGTCCCAGAC
CTTCCTCGCCATCGTGTTCGCGAAAAGCCTCGCGGTGCTCGGCCTCCTGCTGCTGCTTCAGGCGGGGCAGGTGTCGTTCG
GCCACGGCATGTTCTTCGCGACCGGCGCCTACACCGTAGCCTTCCTCGGCCGCAGCGTGGGCGGCGGCGACATAGCCCTG
CTGCTCGCCGCCTCGGCGGTCTCCAGCGTGCTCCTCGGCCTGCTGGTGGGGCTGTTCGTGGTGCGCTACCGCTACATCTT
CTTCGGCATGCTGAATTTGGCCTTCTCCATGGTGCTCTATTCCATCCTGGAGAAGTTCTTCCACCTCACCGGCGGCTCCG
ATGGCCTGCGCATCCGCCGGCCCTCGGCCTTCGGCTTCGCCTTCGATCGCGCGCAGTTCGACACGCTGATCTATTACCTC
ACGCTGGTGCTCGCCTTCGGCGCGGCCTACCTCGTGTGGCGCTATCTGCGCAGCCCGCTCGGACAGGCGCTCAAAGCCAT
CAAGAGCAACGAGACGCGGCTCGAATACATCGGCCTGTCCGCCCGCTTCGTCATGCTGGTGGGCTATGTGATCTCCGCGC
TCCTGTGCGGCATCGGCGGCGCCTGCCTCGGCATCATCCAGGGCATCGCGACGCCCGAGTACAGCTATTGGACCCGCTCC
GCCGAATTCGTCTTCATCGCCATCCTCGGCGGGGTGGGCCACGTGGCCGGCGCGCTCACCGGCACCTTCGTCTACGAGGC
GGTGCGCACCTATGCGGCGGCCTTCGTGGCTGACAGCTGGCAGATGATCCTCGGCTTCGTGCTGCTGGCCATCATCCTGA
AGGCGCCCACCGGCATCGTCGGGCTGGTGCAGGCCATCGCCGGCCGCCGCAAGGCGCAGGCCACGGCCGCCCTCACCGTG
GAGACCGCGCGATGA

Upstream 100 bases:

>100_bases
CCGAATTCGAGGTGCTCGTGCCCTACATCCTCATGGTCGCGGTGCTCATCTTCCGTCCGAGCGGCCTGTTCGGCGCGCCC
CAGCTCAGGAAGATCTGAAA

Downstream 100 bases:

>100_bases
GCGAGCATCCCATCCTGCTTCAGGCGCGCGGTCTCGAGATCCGCTTCGGCGGCGTGGTCGCGGCCAACGGCATCGATCTC
GACGTGCGCGGCGGCGAGAA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAVRWILLALLLAVLAAVPAFAASSQTFLAIVFAKSLAVLGLLLLLQAGQVSFGHGMFFATGAYTVAFLGRSVGGGDIAL
LLAASAVSSVLLGLLVGLFVVRYRYIFFGMLNLAFSMVLYSILEKFFHLTGGSDGLRIRRPSAFGFAFDRAQFDTLIYYL
TLVLAFGAAYLVWRYLRSPLGQALKAIKSNETRLEYIGLSARFVMLVGYVISALLCGIGGACLGIIQGIATPEYSYWTRS
AEFVFIAILGGVGHVAGALTGTFVYEAVRTYAAAFVADSWQMILGFVLLAIILKAPTGIVGLVQAIAGRRKAQATAALTV
ETAR

Sequences:

>Translated_324_residues
MAVRWILLALLLAVLAAVPAFAASSQTFLAIVFAKSLAVLGLLLLLQAGQVSFGHGMFFATGAYTVAFLGRSVGGGDIAL
LLAASAVSSVLLGLLVGLFVVRYRYIFFGMLNLAFSMVLYSILEKFFHLTGGSDGLRIRRPSAFGFAFDRAQFDTLIYYL
TLVLAFGAAYLVWRYLRSPLGQALKAIKSNETRLEYIGLSARFVMLVGYVISALLCGIGGACLGIIQGIATPEYSYWTRS
AEFVFIAILGGVGHVAGALTGTFVYEAVRTYAAAFVADSWQMILGFVLLAIILKAPTGIVGLVQAIAGRRKAQATAALTV
ETAR
>Mature_323_residues
AVRWILLALLLAVLAAVPAFAASSQTFLAIVFAKSLAVLGLLLLLQAGQVSFGHGMFFATGAYTVAFLGRSVGGGDIALL
LAASAVSSVLLGLLVGLFVVRYRYIFFGMLNLAFSMVLYSILEKFFHLTGGSDGLRIRRPSAFGFAFDRAQFDTLIYYLT
LVLAFGAAYLVWRYLRSPLGQALKAIKSNETRLEYIGLSARFVMLVGYVISALLCGIGGACLGIIQGIATPEYSYWTRSA
EFVFIAILGGVGHVAGALTGTFVYEAVRTYAAAFVADSWQMILGFVLLAIILKAPTGIVGLVQAIAGRRKAQATAALTVE
TAR

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=284, Percent_Identity=28.5211267605634, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34544; Mature: 34412

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRWILLALLLAVLAAVPAFAASSQTFLAIVFAKSLAVLGLLLLLQAGQVSFGHGMFFA
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
TGAYTVAFLGRSVGGGDIALLLAASAVSSVLLGLLVGLFVVRYRYIFFGMLNLAFSMVLY
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILEKFFHLTGGSDGLRIRRPSAFGFAFDRAQFDTLIYYLTLVLAFGAAYLVWRYLRSPL
HHHHHHHHHCCCCCCCEEECCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQALKAIKSNETRLEYIGLSARFVMLVGYVISALLCGIGGACLGIIQGIATPEYSYWTRS
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCC
AEFVFIAILGGVGHVAGALTGTFVYEAVRTYAAAFVADSWQMILGFVLLAIILKAPTGIV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
GLVQAIAGRRKAQATAALTVETAR
HHHHHHHCCHHHHHHHEEEEECCC
>Mature Secondary Structure 
AVRWILLALLLAVLAAVPAFAASSQTFLAIVFAKSLAVLGLLLLLQAGQVSFGHGMFFA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH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HHHHHHHCCHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]