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The map label for this gene is gatA2 [H]

Identifier: 154245271

GI number: 154245271

Start: 1518073

End: 1519338

Strand: Reverse

Name: gatA2 [H]

Synonym: Xaut_1323

Alternate gene names: 154245271

Gene position: 1519338-1518073 (Counterclockwise)

Preceding gene: 154245272

Following gene: 154245270

Centisome position: 28.62

GC content: 74.09

Gene sequence:

>1266_bases
ATGACCCTCCTTTTCGCCCGCGACCTTGCCGCCGACCTCCACGCCGGGCGCACCACGCCCCGCGCCGTGCTGGAACAGGT
GGCGCGGGCCGTCGGCGCCCGCGAGAGCGAGGTGCAGGCCTTCGCCGCCCTCGACCTCGACCGGGCGCGCGCGGCGGCGG
ATCAGGCCGGGCTCACGGCGCGCGCCCTCGCCGGCCTGCCGGTGGGGGTGAAGGACATCATCGACACGCGGGACCTGCCC
ACCGCCTACGGCAGCGCGCTCTATGCCGGCCACCGGCCGCGCAACGACGCCCCGGTAGTGGCCATGACGCGCCGGGCCGG
CGGCCTCGTGGTCGGCAAGACCGTGACCACGGAATTTGCCTTCCTGAAGCCATCGCAGACCCGCAACCCGCGCCGGCTCA
CCCATTCGCCGGGCGGCTCCTCGGCGGGCTCGGCGGCGGCGGTGGCGGCGGGCATGCTGCCCCTGGCGATCGGCACCCAG
ACCGCCGGCTCGGTCATCCGCCCCGCCGCCTTCTGCGGGGTCACCGGCTACAAGCCCACCTTCCGCCTGTTGCCCACGCT
GGGGATGAAGACCTTCTCCTGGCACCTCGACACCATGGGCCTGTTCGCCGCCCGGGTGCGGGACGCCGCCTTTGCCGCCG
CCGCCATCACCGGCCGCGACCTCGACATCGGCGAGGAGACCGCAGAGCGACCGCGCATCGGCATCGTGCGCACCGCGCGC
GCCGGTAGTGCCTCCGCCGAGGCCCATGCCGCGCTGGACGCCGCCGCCCGCGCCGCCGAGGCCAGGGGCGCGCGCGTCTT
CGACCTCGACCTGCCCGAAGAGCTGGAGGCCGCCGACGCCGCCCAGCCCATTATCCAGGATTTCGAGGCCGCCCTCGCCT
TCGCCGACGAGCTGGCCTACGACCGCGCCGGCCTCTCGCCGCTGCTCGCCGCCCATCTCGATGCCGCCACCCAGATCGCG
CCCGACACCTACGATCAGGCGCGGCGCACCGCCAAGCGCGCACGGCAGAAGCTGGGCGATGTGTTCGGTGAGGTGGACGC
GCTCCTCACCTTCTCCGCCCCCGGGCCGGCACCGGAGGGCTTTGCCACCACCGGCTCGGCCATGTTCTGCCGGCTGTGGA
CGCTGCTGGGCTGTCCGGCCATCAACATTGCCGGCCTTGAGACGGCGGACGGACTGCCCGTGGGCGTGCAGCTGGTGGGC
CGGTTCGGCCGCGATCGGCAGGCATTGGCGGTGGCGAATCTGCTGGAAAGCGCCATCTCCCGCTGA

Upstream 100 bases:

>100_bases
AAGCGCATCCTGCCGGTGCAGTAGGGCCCCCGCCCCCATTGCGCGCACGCTGCCGCCTGCGCATGGTGGGCGCCCTTTGC
GCCATGCCTCGAGTAACCCC

Downstream 100 bases:

>100_bases
AATACAATATTTCACAAACTTTTCTCGACACCGCCGCTTGCGCCGCAGCGTCGAGAAAAAGGCAGAACCGGGGCACACTT
GGCTTCCGACAGTCATGCGT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTLLFARDLAADLHAGRTTPRAVLEQVARAVGARESEVQAFAALDLDRARAAADQAGLTARALAGLPVGVKDIIDTRDLP
TAYGSALYAGHRPRNDAPVVAMTRRAGGLVVGKTVTTEFAFLKPSQTRNPRRLTHSPGGSSAGSAAAVAAGMLPLAIGTQ
TAGSVIRPAAFCGVTGYKPTFRLLPTLGMKTFSWHLDTMGLFAARVRDAAFAAAAITGRDLDIGEETAERPRIGIVRTAR
AGSASAEAHAALDAAARAAEARGARVFDLDLPEELEAADAAQPIIQDFEAALAFADELAYDRAGLSPLLAAHLDAATQIA
PDTYDQARRTAKRARQKLGDVFGEVDALLTFSAPGPAPEGFATTGSAMFCRLWTLLGCPAINIAGLETADGLPVGVQLVG
RFGRDRQALAVANLLESAISR

Sequences:

>Translated_421_residues
MTLLFARDLAADLHAGRTTPRAVLEQVARAVGARESEVQAFAALDLDRARAAADQAGLTARALAGLPVGVKDIIDTRDLP
TAYGSALYAGHRPRNDAPVVAMTRRAGGLVVGKTVTTEFAFLKPSQTRNPRRLTHSPGGSSAGSAAAVAAGMLPLAIGTQ
TAGSVIRPAAFCGVTGYKPTFRLLPTLGMKTFSWHLDTMGLFAARVRDAAFAAAAITGRDLDIGEETAERPRIGIVRTAR
AGSASAEAHAALDAAARAAEARGARVFDLDLPEELEAADAAQPIIQDFEAALAFADELAYDRAGLSPLLAAHLDAATQIA
PDTYDQARRTAKRARQKLGDVFGEVDALLTFSAPGPAPEGFATTGSAMFCRLWTLLGCPAINIAGLETADGLPVGVQLVG
RFGRDRQALAVANLLESAISR
>Mature_420_residues
TLLFARDLAADLHAGRTTPRAVLEQVARAVGARESEVQAFAALDLDRARAAADQAGLTARALAGLPVGVKDIIDTRDLPT
AYGSALYAGHRPRNDAPVVAMTRRAGGLVVGKTVTTEFAFLKPSQTRNPRRLTHSPGGSSAGSAAAVAAGMLPLAIGTQT
AGSVIRPAAFCGVTGYKPTFRLLPTLGMKTFSWHLDTMGLFAARVRDAAFAAAAITGRDLDIGEETAERPRIGIVRTARA
GSASAEAHAALDAAARAAEARGARVFDLDLPEELEAADAAQPIIQDFEAALAFADELAYDRAGLSPLLAAHLDAATQIAP
DTYDQARRTAKRARQKLGDVFGEVDALLTFSAPGPAPEGFATTGSAMFCRLWTLLGCPAINIAGLETADGLPVGVQLVGR
FGRDRQALAVANLLESAISR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=493, Percent_Identity=23.9350912778905, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI195972892, Length=161, Percent_Identity=29.1925465838509, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=450, Percent_Identity=25.7777777777778, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=380, Percent_Identity=28.1578947368421, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=307, Percent_Identity=24.4299674267101, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550774, Length=398, Percent_Identity=24.6231155778894, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648435, Length=398, Percent_Identity=24.6231155778894, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648437, Length=398, Percent_Identity=24.6231155778894, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648441, Length=398, Percent_Identity=24.6231155778894, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648439, Length=398, Percent_Identity=24.6231155778894, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648113, Length=486, Percent_Identity=26.5432098765432, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 43842; Mature: 43711

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLFARDLAADLHAGRTTPRAVLEQVARAVGARESEVQAFAALDLDRARAAADQAGLTA
CEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
RALAGLPVGVKDIIDTRDLPTAYGSALYAGHRPRNDAPVVAMTRRAGGLVVGKTVTTEFA
HHHHCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEECEECCEEE
FLKPSQTRNPRRLTHSPGGSSAGSAAAVAAGMLPLAIGTQTAGSVIRPAAFCGVTGYKPT
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCH
FRLLPTLGMKTFSWHLDTMGLFAARVRDAAFAAAAITGRDLDIGEETAERPRIGIVRTAR
HHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEECC
AGSASAEAHAALDAAARAAEARGARVFDLDLPEELEAADAAQPIIQDFEAALAFADELAY
CCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRAGLSPLLAAHLDAATQIAPDTYDQARRTAKRARQKLGDVFGEVDALLTFSAPGPAPEG
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
FATTGSAMFCRLWTLLGCPAINIAGLETADGLPVGVQLVGRFGRDRQALAVANLLESAIS
CCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHC
R
C
>Mature Secondary Structure 
TLLFARDLAADLHAGRTTPRAVLEQVARAVGARESEVQAFAALDLDRARAAADQAGLTA
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
RALAGLPVGVKDIIDTRDLPTAYGSALYAGHRPRNDAPVVAMTRRAGGLVVGKTVTTEFA
HHHHCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEECEECCEEE
FLKPSQTRNPRRLTHSPGGSSAGSAAAVAAGMLPLAIGTQTAGSVIRPAAFCGVTGYKPT
EECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCH
FRLLPTLGMKTFSWHLDTMGLFAARVRDAAFAAAAITGRDLDIGEETAERPRIGIVRTAR
HHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEECC
AGSASAEAHAALDAAARAAEARGARVFDLDLPEELEAADAAQPIIQDFEAALAFADELAY
CCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRAGLSPLLAAHLDAATQIAPDTYDQARRTAKRARQKLGDVFGEVDALLTFSAPGPAPEG
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
FATTGSAMFCRLWTLLGCPAINIAGLETADGLPVGVQLVGRFGRDRQALAVANLLESAIS
CCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]