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The map label for this gene is menE [H]

Identifier: 154245234

GI number: 154245234

Start: 1478432

End: 1479955

Strand: Reverse

Name: menE [H]

Synonym: Xaut_1286

Alternate gene names: 154245234

Gene position: 1479955-1478432 (Counterclockwise)

Preceding gene: 154245235

Following gene: 154245233

Centisome position: 27.88

GC content: 73.23

Gene sequence:

>1524_bases
ATGCACCGCTTTTTTGAGGCGGTGGCGTGCCATGCGCGCCGCGAACCCGACCGCATCCTGTTCACCGACGCCGCCGGCAG
CCTGACCCGGGCCGGCCTGATGGCCGACGCGGCGCGCCTTGCCGCCCGGCTGCCGCCGCAGGCCCGCGTCATCGGCCTGC
TCTTGCCCAACGGGCGGGAATGGGCAGTGGCGCAGATGGCCTGCCTTGCCGCCGGCCGCATGGCGGTGCCGCTGCCTACC
TTCTTCAGCGCCCTGCAATTGAGCCATATCCTCATGGACGCGCAGGTGGATCTCGTCCTGGTCCCGCAGGCGAACCCGCC
CGCACTGCCTGCCGCCGTGCCCGTGCTGCCGGTGGCCGCCAGCGGCGAAAGCGGCGCCATGCCGGACTTTAGCGAGGGGT
TCGGCCAGATTATCTACACCTCCGGCAGCTCCGGCCAGCCGAAGGGCGTGCGCCACCAGAGCGGGCAGATCGCCTGGTCG
GCGTCGGCGCTGGCTGAGGCCATCGCGGCCGATGCGGAAGACACCTATCTGTCCGTCCTGCCCCTGTCGCTGCTGCTTGA
AAGCATCTGCGCCGTGTTCGTTCCCGCGCTGGTGGGCGGGCGCACCCACTTCGACACCCAGCTCTCGGAGGCCATCGGAC
GCGGCGCGGTGCGTGGCGTCGCCGCCGCGTTCGAGGCCCATCGGCCGAGCGTGGGCGTGGTGGTGCCCGAGCTGCTGCGG
GTGTGGGCCCACGACCTCCTCGCCGCGGGCCGCAGGGCGCCGGACAGCCTGCGCTTCGTCGCCGCCGGCGGCGCCCGGGT
GCCGGAACGCCTCGCCGAGACGGCCTGGCAGCTCGGCATTCCGGTGCATGAGGGCTATGGCCTCTCCGAATGCTGCTCCG
TGGTGGCGCTGAACCGGCCCGGTGCCCGCGTGGCGGGAACCGTGGGGGCGCCGCTGCCCGGCCTCCAGCTCACCATCCGC
GACGGCGAGATCCTGGTGGACGGCCCCGGCGTGATGGACGGCTATCTCGGCGGCCCGGAAGCCCCGCGCCCGTGGCCCAC
GGGCGATCTCGGCGCGCTGGATGGCCAGGGGCGCCTCACCGTGTTCGGCCGCAAGGACAACCTCATCGTCACCCCGCTGG
GGCGGAACATCAGTCCGGAATGGGTGGAGACCGCCCTTCTGGACGACCCCGGCATCGCCGCCTGTGCGGTGGCGCAGGTG
GATTTGGTGGAGACAGATGGGTCGGAGGCGGACGGCGCCCTCGCCGCCCTCGTCATCCCCACGTCGCAGGCACAGGGCTG
GTTCGACGATGCCGGCCCGCAGGGCATCCGCGCGCGCATCGCCGAACGCTGCGCCGCCCTGCCCGTCTATGCCCGGCCCA
CGCGCATCACGGTGCTGGGGCTTGCCGACGCCAGGGCGAGGGGGGTCCTCACCGCCAACGGCCGCATCCGCCGTCCCGCC
GCCCGCGCCGTGCTGGCGCAGAGGACCGGCGCCAACCCGTGCCCCGTTCCTCCCCTTCCCTCCCAGGAAAGGCCCCTGCC
ATGA

Upstream 100 bases:

>100_bases
GCCGCCTGGGGGCGCTATTACGAAACCGAGCCCAAGGTCTATGGCGTGTGCGGCGCACGGCTCACCGGCCGCAGCGCCCG
CGTGGTGGAGGCATCGCACC

Downstream 100 bases:

>100_bases
CGTTTTACGACCGCCTCATCCGGGAGACCGCGCGCGAGCGCGATGCCTTCCTCGCCATCCCCCTCGTGCGGCAGGCGCTC
GCCTCCGGCGCGCCGAAAAG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MHRFFEAVACHARREPDRILFTDAAGSLTRAGLMADAARLAARLPPQARVIGLLLPNGREWAVAQMACLAAGRMAVPLPT
FFSALQLSHILMDAQVDLVLVPQANPPALPAAVPVLPVAASGESGAMPDFSEGFGQIIYTSGSSGQPKGVRHQSGQIAWS
ASALAEAIAADAEDTYLSVLPLSLLLESICAVFVPALVGGRTHFDTQLSEAIGRGAVRGVAAAFEAHRPSVGVVVPELLR
VWAHDLLAAGRRAPDSLRFVAAGGARVPERLAETAWQLGIPVHEGYGLSECCSVVALNRPGARVAGTVGAPLPGLQLTIR
DGEILVDGPGVMDGYLGGPEAPRPWPTGDLGALDGQGRLTVFGRKDNLIVTPLGRNISPEWVETALLDDPGIAACAVAQV
DLVETDGSEADGALAALVIPTSQAQGWFDDAGPQGIRARIAERCAALPVYARPTRITVLGLADARARGVLTANGRIRRPA
ARAVLAQRTGANPCPVPPLPSQERPLP

Sequences:

>Translated_507_residues
MHRFFEAVACHARREPDRILFTDAAGSLTRAGLMADAARLAARLPPQARVIGLLLPNGREWAVAQMACLAAGRMAVPLPT
FFSALQLSHILMDAQVDLVLVPQANPPALPAAVPVLPVAASGESGAMPDFSEGFGQIIYTSGSSGQPKGVRHQSGQIAWS
ASALAEAIAADAEDTYLSVLPLSLLLESICAVFVPALVGGRTHFDTQLSEAIGRGAVRGVAAAFEAHRPSVGVVVPELLR
VWAHDLLAAGRRAPDSLRFVAAGGARVPERLAETAWQLGIPVHEGYGLSECCSVVALNRPGARVAGTVGAPLPGLQLTIR
DGEILVDGPGVMDGYLGGPEAPRPWPTGDLGALDGQGRLTVFGRKDNLIVTPLGRNISPEWVETALLDDPGIAACAVAQV
DLVETDGSEADGALAALVIPTSQAQGWFDDAGPQGIRARIAERCAALPVYARPTRITVLGLADARARGVLTANGRIRRPA
ARAVLAQRTGANPCPVPPLPSQERPLP
>Mature_507_residues
MHRFFEAVACHARREPDRILFTDAAGSLTRAGLMADAARLAARLPPQARVIGLLLPNGREWAVAQMACLAAGRMAVPLPT
FFSALQLSHILMDAQVDLVLVPQANPPALPAAVPVLPVAASGESGAMPDFSEGFGQIIYTSGSSGQPKGVRHQSGQIAWS
ASALAEAIAADAEDTYLSVLPLSLLLESICAVFVPALVGGRTHFDTQLSEAIGRGAVRGVAAAFEAHRPSVGVVVPELLR
VWAHDLLAAGRRAPDSLRFVAAGGARVPERLAETAWQLGIPVHEGYGLSECCSVVALNRPGARVAGTVGAPLPGLQLTIR
DGEILVDGPGVMDGYLGGPEAPRPWPTGDLGALDGQGRLTVFGRKDNLIVTPLGRNISPEWVETALLDDPGIAACAVAQV
DLVETDGSEADGALAALVIPTSQAQGWFDDAGPQGIRARIAERCAALPVYARPTRITVLGLADARARGVLTANGRIRRPA
ARAVLAQRTGANPCPVPPLPSQERPLP

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=463, Percent_Identity=27.2138228941685, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI187761343, Length=463, Percent_Identity=27.2138228941685, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI40807491, Length=500, Percent_Identity=23.6, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI57165412, Length=499, Percent_Identity=24.0480961923848, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI57165410, Length=499, Percent_Identity=23.8476953907816, Blast_Score=93, Evalue=7e-19,
Organism=Escherichia coli, GI145693145, Length=542, Percent_Identity=24.9077490774908, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789201, Length=326, Percent_Identity=27.9141104294479, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788107, Length=160, Percent_Identity=32.5, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788595, Length=263, Percent_Identity=28.8973384030418, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52802; Mature: 52802

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRFFEAVACHARREPDRILFTDAAGSLTRAGLMADAARLAARLPPQARVIGLLLPNGRE
CCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
WAVAQMACLAAGRMAVPLPTFFSALQLSHILMDAQVDLVLVPQANPPALPAAVPVLPVAA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEE
SGESGAMPDFSEGFGQIIYTSGSSGQPKGVRHQSGQIAWSASALAEAIAADAEDTYLSVL
CCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCCHHHHHH
PLSLLLESICAVFVPALVGGRTHFDTQLSEAIGRGAVRGVAAAFEAHRPSVGVVVPELLR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VWAHDLLAAGRRAPDSLRFVAAGGARVPERLAETAWQLGIPVHEGYGLSECCSVVALNRP
HHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHEEEECCC
GARVAGTVGAPLPGLQLTIRDGEILVDGPGVMDGYLGGPEAPRPWPTGDLGALDGQGRLT
CCCEEECCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
VFGRKDNLIVTPLGRNISPEWVETALLDDPGIAACAVAQVDLVETDGSEADGALAALVIP
EEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEEEE
TSQAQGWFDDAGPQGIRARIAERCAALPVYARPTRITVLGLADARARGVLTANGRIRRPA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCEEEEEEECCCCCCEEEECCCCEECHH
ARAVLAQRTGANPCPVPPLPSQERPLP
HHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHRFFEAVACHARREPDRILFTDAAGSLTRAGLMADAARLAARLPPQARVIGLLLPNGRE
CCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
WAVAQMACLAAGRMAVPLPTFFSALQLSHILMDAQVDLVLVPQANPPALPAAVPVLPVAA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEE
SGESGAMPDFSEGFGQIIYTSGSSGQPKGVRHQSGQIAWSASALAEAIAADAEDTYLSVL
CCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCCHHHHHH
PLSLLLESICAVFVPALVGGRTHFDTQLSEAIGRGAVRGVAAAFEAHRPSVGVVVPELLR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VWAHDLLAAGRRAPDSLRFVAAGGARVPERLAETAWQLGIPVHEGYGLSECCSVVALNRP
HHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHEEEECCC
GARVAGTVGAPLPGLQLTIRDGEILVDGPGVMDGYLGGPEAPRPWPTGDLGALDGQGRLT
CCCEEECCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
VFGRKDNLIVTPLGRNISPEWVETALLDDPGIAACAVAQVDLVETDGSEADGALAALVIP
EEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEEEE
TSQAQGWFDDAGPQGIRARIAERCAALPVYARPTRITVLGLADARARGVLTANGRIRRPA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCEEEEEEECCCCCCEEEECCCCEECHH
ARAVLAQRTGANPCPVPPLPSQERPLP
HHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377 [H]