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The map label for this gene is rutF2 [H]

Identifier: 154245160

GI number: 154245160

Start: 1394225

End: 1394782

Strand: Reverse

Name: rutF2 [H]

Synonym: Xaut_1212

Alternate gene names: 154245160

Gene position: 1394782-1394225 (Counterclockwise)

Preceding gene: 154245162

Following gene: 154245159

Centisome position: 26.27

GC content: 72.76

Gene sequence:

>558_bases
ATGAACGCGCATACGCCCGTCGCCGCCCCCACCCCCTCGCCCACCCAATCGCCGAGCCTTGCCCCCGAGCTGCCGGACGC
CTCGCTCGCCTTCCGCGCCGCCATGCGCAAGCTGGCCGGGGCCGTGAGCGTGCTCACCGTCGGCACCGGGGACGAGCGCA
CCGGGCTCACCGCCACCTCGGTCACCTCGCTTTCGGTGGAGCCGCCGACCCTGCTCATTGCCGTCAACCGCGGCTCCTCG
TCCTTCGCCCCGCTGCTGAAGGAGCGCAGCTTCGCCATCAACGTGCTGCGCCCCCACCACCAGCCCATCGCCGACCAGTT
CGCCGGCAAGGGCGGCCTGAAGGGACCCGCGCGCTACCAGGGCGCCGACTGGACCAAGCTGGAAACCGGCGCGCCGGTGC
TGGCGGACGCGCTGGCCGCCTTCGACTGCGAGCTGGAGGACGCCATCGAGCGGCACTCCCACGTCATCGTCATCGGTCGC
GTGGTGGCCTCGCGCATCACCGAGAGCGCGCAGCCGCTGCTCTACTGGGCCGGCGGCTATCGCGGCCTCGCCGACTGA

Upstream 100 bases:

>100_bases
TGGCCGCTGTCTATCCTTTTTTCCACCAGACGCCCTCCGGATTGATCCGGAGGCTCAAGAACGCCCCCGGCGCCGCCGCG
CCGACCAGAAGGCCCTTTCC

Downstream 100 bases:

>100_bases
CGCCGGCCCGCCGGCCGCCCCCGCATTTCTCCCGAAGGATCACCTCATGACCCACCGCGTCACCCGCCGCACCTCGCTCC
TCCTCGCAGCCGGCCTTGCG

Product: flavin reductase domain-containing protein

Products: NA

Alternate protein names: FMN reductase 2; NAD(P)H:flavin oxidoreductase 2 [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MNAHTPVAAPTPSPTQSPSLAPELPDASLAFRAAMRKLAGAVSVLTVGTGDERTGLTATSVTSLSVEPPTLLIAVNRGSS
SFAPLLKERSFAINVLRPHHQPIADQFAGKGGLKGPARYQGADWTKLETGAPVLADALAAFDCELEDAIERHSHVIVIGR
VVASRITESAQPLLYWAGGYRGLAD

Sequences:

>Translated_185_residues
MNAHTPVAAPTPSPTQSPSLAPELPDASLAFRAAMRKLAGAVSVLTVGTGDERTGLTATSVTSLSVEPPTLLIAVNRGSS
SFAPLLKERSFAINVLRPHHQPIADQFAGKGGLKGPARYQGADWTKLETGAPVLADALAAFDCELEDAIERHSHVIVIGR
VVASRITESAQPLLYWAGGYRGLAD
>Mature_185_residues
MNAHTPVAAPTPSPTQSPSLAPELPDASLAFRAAMRKLAGAVSVLTVGTGDERTGLTATSVTSLSVEPPTLLIAVNRGSS
SFAPLLKERSFAINVLRPHHQPIADQFAGKGGLKGPARYQGADWTKLETGAPVLADALAAFDCELEDAIERHSHVIVIGR
VVASRITESAQPLLYWAGGYRGLAD

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=153, Percent_Identity=30.718954248366, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 19359; Mature: 19359

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHTPVAAPTPSPTQSPSLAPELPDASLAFRAAMRKLAGAVSVLTVGTGDERTGLTATS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEE
VTSLSVEPPTLLIAVNRGSSSFAPLLKERSFAINVLRPHHQPIADQFAGKGGLKGPARYQ
EEEEECCCCEEEEEEECCCCHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCC
GADWTKLETGAPVLADALAAFDCELEDAIERHSHVIVIGRVVASRITESAQPLLYWAGGY
CCCCCEECCCCCHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHHHHHCCCCEEEECCCC
RGLAD
CCCCC
>Mature Secondary Structure
MNAHTPVAAPTPSPTQSPSLAPELPDASLAFRAAMRKLAGAVSVLTVGTGDERTGLTATS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEE
VTSLSVEPPTLLIAVNRGSSSFAPLLKERSFAINVLRPHHQPIADQFAGKGGLKGPARYQ
EEEEECCCCEEEEEEECCCCHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCC
GADWTKLETGAPVLADALAAFDCELEDAIERHSHVIVIGRVVASRITESAQPLLYWAGGY
CCCCCEECCCCCHHHHHHHHHCCCHHHHHHHCCCEEEEHHHHHHHHHHCCCCEEEECCCC
RGLAD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA