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The map label for this gene is meaA [H]

Identifier: 154245145

GI number: 154245145

Start: 1376066

End: 1378039

Strand: Direct

Name: meaA [H]

Synonym: Xaut_1197

Alternate gene names: 154245145

Gene position: 1376066-1378039 (Clockwise)

Preceding gene: 154245144

Following gene: 154245146

Centisome position: 25.92

GC content: 67.33

Gene sequence:

>1974_bases
ATGGTGGCGCGCGACAAACCCTGGCTTTTCCGCACCTATGCGGGCCACTCCACCGCCACGGAGTCCAACAAGCTCTACCG
TTCCAACCTCGCCAAGGGCCAGACCGGCCTCTCGGTCGCCTTCGACCTGCCCACCCAGACCGGCTATGATTCGGACCACC
CGCTGGCGCGCGGCGAGGTGGGCAAGGTGGGCGTGCCGATCTCCCATCTCGGCGACATGCGCACCCTGTTCGACGGCATC
CCGCTCGCCGAGATGAACACGTCGATGACCATCAACGCCTGCGCGGCGTGGCTGCTCTCGCTCTATATCGCCACCGCCGA
CGAGCAGGGCGCCGACCGCACCAAGCTGCAGGGCACCACCCAGAACGACATCATCAAGGAATATCTGTCGCGCGGCACCT
ATGTGTTCCCGCCGGCGCCGTCCATGCGGCTGACCAAGGACACCATCATCTTCACCACTGAGTATCTGCCCCGGTGGAAC
CCGATGAACGTGTGCTCCTACCACCTGCAGGAGGCCGGGGCGACGCCGGTGCAGGAGCTGGCCTTCGCGCTCGCCAACGC
CATCGCCATCCTCGACACGGTGAAGAGGTCGGGCGAGGCGGAAGGCAAGGTGTTCGGCGAGGTGGTGGGGCGCATCTCCT
TCTTCGTGAACGCCGGCATGCGGTTCATCACCGAGATGTGCAAGATGCGCGCGTTCGTGGATCTGTGGGACGAGATCTGC
GTCAACCGCTATGGCATCACCGACGCCAAGCAGAAGCTGTTCCGCTACGGCGTGCAGGTGAATTCGCTGGGCCTCACTGA
GCAGCAGCCGGAGAACAACGTCTACCGCATCCTGCTGGAGATGCTGGCGGTCACCCTGTCGAAGGGCGCCCGCGCCCGCG
CCGTGCAGCTTCCCGCGTGGAACGAGGCGCTGGGCCTGCCCCGCTCGTTCGACCAGCAATGGTCGCTGCGCATGCAGCAG
GTGCTGGCCTATGAGACCGACCTGCTCGAATACGGCGACATCTTCGACGGCTCCATCGAGATCGCGAGGAAGGTGGAGCA
GCTGAAGGAAGAGGCCCGCGCAGAGCTGGCGCAGATCGACGCCATGGGCGGCGCGGTGGCGGCCATCGAGAATTCCTACA
TGAAGCAGCAGCTGGTGGAATCCAACACCCGCCGGCTGGAGGCCATCGAGCGCGGCGAGCAGGTAGTGGTGGGCGTCAAC
CGCTGGACCGAGACCGAGCCTTCGCCGCTCACCACCGGGGAAGGCGCCATCCTCACGGTGCCCGAGGGCGTGGAGGCTGA
GCAGATCGCCCGCCTTCAGGCCTGGCGCGAGGCCCGCGACAGTAAGGCGGTGGCGGCGGCCCTCGCAGCGCTGAAGAGCG
CGGCGCAGGAGGGGCGCAACATCATGGAGCCCTCCATCGTGTGCGCCAAGGCCGGCATCACCACCGGCGAATGGGGCACC
TGCCTGCGCGAGGTGTTCGGCGAATATCGCGCGCCCACCGGTGTCGGCCGTGCCGCCCGGGTGGACACGCAGGGGCTGGA
CGAGGTGCGCAAGGGCGTGGACGCGGTGTCCGAGAAGATCGGCCGGCGCATCAAGTTCCTGGTGGGTAAGCCGGGCCTCG
ACGGCCATTCCAACGGCGCCGAGCAGATCGCCGTGCGCGCCCGCGACTGCGGCATGGAAGTGGTCTACGAGGGCATCCGC
CTGACCCCCGCCGAGATCGTGAACGCCGCGCTGGAAGAAAGCGTGCACGTCATCGGCCTCTCCATCCTTTCCGGCTCCCA
CGTGCCGCTGGTGCGCGACGTGATGGACCGCCTGCGGGCCGAGGGGCTGTCCGACATCCCCGTGGTGGTGGGCGGCATCA
TCCCGCCGGAGGACGAGGTGCAGCTCAAGGGCTTCGGCGTGACCGCCGTCTACACCCCGAAGAACTTCGAGCTGAACCGC
ATCATGGCCGACATCGTCGCCATCGTGGACCGGCAGGCCCAGAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGAGCAGTGCCGTCGACCTCCACCAATTTCCCATCGACGTCCGGGCCATTGCGACGCACAATCGCGTCCAATCCAGAAC
GACCAGATGGGGGAACAGGA

Downstream 100 bases:

>100_bases
GCCGGGCGCGCCGGGGAAAGGTTTCGCCGATCAGCGTGCGATGTAGGCTGATCGGCGCCTTTGACAGGTCGGTGCTCGAT
TTGCCGGGAGAGGGCGCATG

Product: methylmalonyl-CoA mutase, large subunit

Products: succinyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MVARDKPWLFRTYAGHSTATESNKLYRSNLAKGQTGLSVAFDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFDGI
PLAEMNTSMTINACAAWLLSLYIATADEQGADRTKLQGTTQNDIIKEYLSRGTYVFPPAPSMRLTKDTIIFTTEYLPRWN
PMNVCSYHLQEAGATPVQELAFALANAIAILDTVKRSGEAEGKVFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEIC
VNRYGITDAKQKLFRYGVQVNSLGLTEQQPENNVYRILLEMLAVTLSKGARARAVQLPAWNEALGLPRSFDQQWSLRMQQ
VLAYETDLLEYGDIFDGSIEIARKVEQLKEEARAELAQIDAMGGAVAAIENSYMKQQLVESNTRRLEAIERGEQVVVGVN
RWTETEPSPLTTGEGAILTVPEGVEAEQIARLQAWREARDSKAVAAALAALKSAAQEGRNIMEPSIVCAKAGITTGEWGT
CLREVFGEYRAPTGVGRAARVDTQGLDEVRKGVDAVSEKIGRRIKFLVGKPGLDGHSNGAEQIAVRARDCGMEVVYEGIR
LTPAEIVNAALEESVHVIGLSILSGSHVPLVRDVMDRLRAEGLSDIPVVVGGIIPPEDEVQLKGFGVTAVYTPKNFELNR
IMADIVAIVDRQAQKAA

Sequences:

>Translated_657_residues
MVARDKPWLFRTYAGHSTATESNKLYRSNLAKGQTGLSVAFDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFDGI
PLAEMNTSMTINACAAWLLSLYIATADEQGADRTKLQGTTQNDIIKEYLSRGTYVFPPAPSMRLTKDTIIFTTEYLPRWN
PMNVCSYHLQEAGATPVQELAFALANAIAILDTVKRSGEAEGKVFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEIC
VNRYGITDAKQKLFRYGVQVNSLGLTEQQPENNVYRILLEMLAVTLSKGARARAVQLPAWNEALGLPRSFDQQWSLRMQQ
VLAYETDLLEYGDIFDGSIEIARKVEQLKEEARAELAQIDAMGGAVAAIENSYMKQQLVESNTRRLEAIERGEQVVVGVN
RWTETEPSPLTTGEGAILTVPEGVEAEQIARLQAWREARDSKAVAAALAALKSAAQEGRNIMEPSIVCAKAGITTGEWGT
CLREVFGEYRAPTGVGRAARVDTQGLDEVRKGVDAVSEKIGRRIKFLVGKPGLDGHSNGAEQIAVRARDCGMEVVYEGIR
LTPAEIVNAALEESVHVIGLSILSGSHVPLVRDVMDRLRAEGLSDIPVVVGGIIPPEDEVQLKGFGVTAVYTPKNFELNR
IMADIVAIVDRQAQKAA
>Mature_657_residues
MVARDKPWLFRTYAGHSTATESNKLYRSNLAKGQTGLSVAFDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFDGI
PLAEMNTSMTINACAAWLLSLYIATADEQGADRTKLQGTTQNDIIKEYLSRGTYVFPPAPSMRLTKDTIIFTTEYLPRWN
PMNVCSYHLQEAGATPVQELAFALANAIAILDTVKRSGEAEGKVFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEIC
VNRYGITDAKQKLFRYGVQVNSLGLTEQQPENNVYRILLEMLAVTLSKGARARAVQLPAWNEALGLPRSFDQQWSLRMQQ
VLAYETDLLEYGDIFDGSIEIARKVEQLKEEARAELAQIDAMGGAVAAIENSYMKQQLVESNTRRLEAIERGEQVVVGVN
RWTETEPSPLTTGEGAILTVPEGVEAEQIARLQAWREARDSKAVAAALAALKSAAQEGRNIMEPSIVCAKAGITTGEWGT
CLREVFGEYRAPTGVGRAARVDTQGLDEVRKGVDAVSEKIGRRIKFLVGKPGLDGHSNGAEQIAVRARDCGMEVVYEGIR
LTPAEIVNAALEESVHVIGLSILSGSHVPLVRDVMDRLRAEGLSDIPVVVGGIIPPEDEVQLKGFGVTAVYTPKNFELNR
IMADIVAIVDRQAQKAA

Specific function: Required for the assimilation of methanol and ethanol [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=634, Percent_Identity=35.8044164037855, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1789284, Length=656, Percent_Identity=38.719512195122, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25144500, Length=633, Percent_Identity=35.3870458135861, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: 5.4.99.2

Molecular weight: Translated: 71937; Mature: 71937

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVARDKPWLFRTYAGHSTATESNKLYRSNLAKGQTGLSVAFDLPTQTGYDSDHPLARGEV
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
GKVGVPISHLGDMRTLFDGIPLAEMNTSMTINACAAWLLSLYIATADEQGADRTKLQGTT
CCCCCCHHHHHHHHHHHCCCCHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHCCCCC
QNDIIKEYLSRGTYVFPPAPSMRLTKDTIIFTTEYLPRWNPMNVCSYHLQEAGATPVQEL
HHHHHHHHHHCCCEEECCCCCCEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHH
AFALANAIAILDTVKRSGEAEGKVFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEIC
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRYGITDAKQKLFRYGVQVNSLGLTEQQPENNVYRILLEMLAVTLSKGARARAVQLPAW
HCCCCCCHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
NEALGLPRSFDQQWSLRMQQVLAYETDLLEYGDIFDGSIEIARKVEQLKEEARAELAQID
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AMGGAVAAIENSYMKQQLVESNTRRLEAIERGEQVVVGVNRWTETEPSPLTTGEGAILTV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEC
PEGVEAEQIARLQAWREARDSKAVAAALAALKSAAQEGRNIMEPSIVCAKAGITTGEWGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHHH
CLREVFGEYRAPTGVGRAARVDTQGLDEVRKGVDAVSEKIGRRIKFLVGKPGLDGHSNGA
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCH
EQIAVRARDCGMEVVYEGIRLTPAEIVNAALEESVHVIGLSILSGSHVPLVRDVMDRLRA
HHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHHHH
EGLSDIPVVVGGIIPPEDEVQLKGFGVTAVYTPKNFELNRIMADIVAIVDRQAQKAA
CCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVARDKPWLFRTYAGHSTATESNKLYRSNLAKGQTGLSVAFDLPTQTGYDSDHPLARGEV
CCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
GKVGVPISHLGDMRTLFDGIPLAEMNTSMTINACAAWLLSLYIATADEQGADRTKLQGTT
CCCCCCHHHHHHHHHHHCCCCHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHCCCCC
QNDIIKEYLSRGTYVFPPAPSMRLTKDTIIFTTEYLPRWNPMNVCSYHLQEAGATPVQEL
HHHHHHHHHHCCCEEECCCCCCEECCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHH
AFALANAIAILDTVKRSGEAEGKVFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEIC
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRYGITDAKQKLFRYGVQVNSLGLTEQQPENNVYRILLEMLAVTLSKGARARAVQLPAW
HCCCCCCHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCC
NEALGLPRSFDQQWSLRMQQVLAYETDLLEYGDIFDGSIEIARKVEQLKEEARAELAQID
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AMGGAVAAIENSYMKQQLVESNTRRLEAIERGEQVVVGVNRWTETEPSPLTTGEGAILTV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEC
PEGVEAEQIARLQAWREARDSKAVAAALAALKSAAQEGRNIMEPSIVCAKAGITTGEWGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHHH
CLREVFGEYRAPTGVGRAARVDTQGLDEVRKGVDAVSEKIGRRIKFLVGKPGLDGHSNGA
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCH
EQIAVRARDCGMEVVYEGIRLTPAEIVNAALEESVHVIGLSILSGSHVPLVRDVMDRLRA
HHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHHHH
EGLSDIPVVVGGIIPPEDEVQLKGFGVTAVYTPKNFELNRIMADIVAIVDRQAQKAA
CCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-methylmalonyl-CoA

Specific reaction: (R)-methylmalonyl-CoA = succinyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]