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The map label for this gene is yogA [H]

Identifier: 154245143

GI number: 154245143

Start: 1374082

End: 1375365

Strand: Reverse

Name: yogA [H]

Synonym: Xaut_1195

Alternate gene names: 154245143

Gene position: 1375365-1374082 (Counterclockwise)

Preceding gene: 154245150

Following gene: 154245142

Centisome position: 25.91

GC content: 66.74

Gene sequence:

>1284_bases
ATGGCCCAGACGGCAGCCGCCAACGCGAACGAGGGACCGGTGAAGGACCTTTATGAGCTGGGCGAGGTTCCCCCCCTCGG
TCACGTCCCCGCCAAGATGTACGCCTGGGCCATCCGCCGCGAGCGCCATGGGCCGCCGGAAGAGTCGTTCCAGCTGGAAG
TGGTGCCCACCTGGGAGCTGGGCGAGAACGACGTGCTGGTCTACGTCATGGCCGCCGGCGTCAACTACAACGGCATCTGG
GCGGGCCTCGGCCAGCCGATCTCGCCGTTCGACGTGCACAAGGCGCCCTTCCACATCGCCGGCTCCGATGCCTCGGGTAT
CGTCTGGGCGGTGGGCTCCAAGGTGAAGCGCTGGAAGGTGGGCGACGAGGTGGTCGTGCACTGTAACCAGGACGACGGCG
ACGACGAGGAGTGCAACGGCGGCGACCCCATGTTCTCCCCGTCCCAGCGCATCTGGGGCTATGAGACGCCGGACGGCTCG
TTCGCCCAGTTCTGCCGGGTGCAGGCGCGCCAGCTGATGCCGCGCCCCAAGCACCTGACCTGGGAAGAGAGCGCCTGCTA
CACCCTCACCATGGCCACCGCCTACCGCATGCTGTTCGGCCATCCGCCGCACACGGTGAAGCCGGGCGACTACGTGCTGG
TGTGGGGCGCCTCGGGCGGCCTCGGCGTGTTCGGCGTGCAGCTCGCCGCCGCCTCCGGCGCCCATGTGATCGGCGTGATC
TCCGACGAGACCAAGCGCGACTATGTCCTCGGCCTCGGCGCCAAGGGCGTGATCAACCGCAAGGATTTCAAGTGCTGGGG
CCAGCTGCCCAAGGTCAACTCGCCGGAATACAATGAGTGGACCAAGGAAGCCCGCAAGTTCGGCAAGGCCATTTGGGACA
TCAGCGGCAAGCGCGACGTGGACATCGTGTTCGAGCATCCTGGCGAGCAGACCTTCCCGGTCTCGACCCTCGTGGGCAAG
CGCGGCGGCATGATCGTGTTCTGCGCCGGCACCACCGGCTTCAACATCACCTTCGACGCCCGCTACGTGTGGATGCGCCA
GAAGCGCATCCAGGGCTCCCACTTCGCTCACCTCAAGCAGGCCTCCGCCGCCAATCAGTTCATCATCGACCGGCGCGTGG
ACCCCTGCATGTCGGAAGTGTTTCCGTGGGACCGCATCCCCGAGGCGCACACCAAGATGTGGAAGAACCAGCACGCCCCT
GGCAACATGGCGGTGCTGGTCAACACCCCCCGCACCGGCCTGCGTACCCTCGAGGACGTGATCGAGGCCGGCGCGAAGAA
GTGA

Upstream 100 bases:

>100_bases
GGGTATCGCTTTGGAATTTTTCTCTGCTACGCTGCAACGCAAGAAGCCCCCAAAGACGCGACCGAACGGTCCGTAAAAAT
GCCAGGAGAGGACGAAAGCC

Downstream 100 bases:

>100_bases
GCCAGACTTCGTAAGCCAGGCTTCATCCAGGACCAGGCCGCGTCCAAGACCCGCGCCGTATTCGGCGCGGGTTACCTGTG
CTAGTGTGCCGTGCAACGTT

Product: crotonyl-CoA reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAQTAAANANEGPVKDLYELGEVPPLGHVPAKMYAWAIRRERHGPPEESFQLEVVPTWELGENDVLVYVMAAGVNYNGIW
AGLGQPISPFDVHKAPFHIAGSDASGIVWAVGSKVKRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGS
FAQFCRVQARQLMPRPKHLTWEESACYTLTMATAYRMLFGHPPHTVKPGDYVLVWGASGGLGVFGVQLAAASGAHVIGVI
SDETKRDYVLGLGAKGVINRKDFKCWGQLPKVNSPEYNEWTKEARKFGKAIWDISGKRDVDIVFEHPGEQTFPVSTLVGK
RGGMIVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASAANQFIIDRRVDPCMSEVFPWDRIPEAHTKMWKNQHAP
GNMAVLVNTPRTGLRTLEDVIEAGAKK

Sequences:

>Translated_427_residues
MAQTAAANANEGPVKDLYELGEVPPLGHVPAKMYAWAIRRERHGPPEESFQLEVVPTWELGENDVLVYVMAAGVNYNGIW
AGLGQPISPFDVHKAPFHIAGSDASGIVWAVGSKVKRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGS
FAQFCRVQARQLMPRPKHLTWEESACYTLTMATAYRMLFGHPPHTVKPGDYVLVWGASGGLGVFGVQLAAASGAHVIGVI
SDETKRDYVLGLGAKGVINRKDFKCWGQLPKVNSPEYNEWTKEARKFGKAIWDISGKRDVDIVFEHPGEQTFPVSTLVGK
RGGMIVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASAANQFIIDRRVDPCMSEVFPWDRIPEAHTKMWKNQHAP
GNMAVLVNTPRTGLRTLEDVIEAGAKK
>Mature_426_residues
AQTAAANANEGPVKDLYELGEVPPLGHVPAKMYAWAIRRERHGPPEESFQLEVVPTWELGENDVLVYVMAAGVNYNGIWA
GLGQPISPFDVHKAPFHIAGSDASGIVWAVGSKVKRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSF
AQFCRVQARQLMPRPKHLTWEESACYTLTMATAYRMLFGHPPHTVKPGDYVLVWGASGGLGVFGVQLAAASGAHVIGVIS
DETKRDYVLGLGAKGVINRKDFKCWGQLPKVNSPEYNEWTKEARKFGKAIWDISGKRDVDIVFEHPGEQTFPVSTLVGKR
GGMIVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQASAANQFIIDRRVDPCMSEVFPWDRIPEAHTKMWKNQHAPG
NMAVLVNTPRTGLRTLEDVIEAGAKK

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=356, Percent_Identity=23.5955056179775, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI13236495, Length=356, Percent_Identity=23.5955056179775, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI194239676, Length=217, Percent_Identity=27.1889400921659, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI47519420, Length=370, Percent_Identity=23.7837837837838, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71988145, Length=228, Percent_Identity=31.140350877193, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17507255, Length=388, Percent_Identity=24.2268041237113, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=282, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323729, Length=206, Percent_Identity=32.5242718446602, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319520, Length=296, Percent_Identity=26.6891891891892, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319621, Length=203, Percent_Identity=32.512315270936, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=361, Percent_Identity=24.6537396121884, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=338, Percent_Identity=24.8520710059172, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 47276; Mature: 47145

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTAAANANEGPVKDLYELGEVPPLGHVPAKMYAWAIRRERHGPPEESFQLEVVPTWEL
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEC
GENDVLVYVMAAGVNYNGIWAGLGQPISPFDVHKAPFHIAGSDASGIVWAVGSKVKRWKV
CCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCEEEEC
GDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFAQFCRVQARQLMPRPKHLT
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCC
WEESACYTLTMATAYRMLFGHPPHTVKPGDYVLVWGASGGLGVFGVQLAAASGAHVIGVI
CCCCCEEEEEHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEE
SDETKRDYVLGLGAKGVINRKDFKCWGQLPKVNSPEYNEWTKEARKFGKAIWDISGKRDV
CCCCCCCEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
DIVFEHPGEQTFPVSTLVGKRGGMIVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQ
EEEEECCCCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEEEEEEEHHHHCCCCHHHHHHH
ASAANQFIIDRRVDPCMSEVFPWDRIPEAHTKMWKNQHAPGNMAVLVNTPRTGLRTLEDV
HHCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHH
IEAGAKK
HHHCCCC
>Mature Secondary Structure 
AQTAAANANEGPVKDLYELGEVPPLGHVPAKMYAWAIRRERHGPPEESFQLEVVPTWEL
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEC
GENDVLVYVMAAGVNYNGIWAGLGQPISPFDVHKAPFHIAGSDASGIVWAVGSKVKRWKV
CCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCEEEEC
GDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFAQFCRVQARQLMPRPKHLT
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCC
WEESACYTLTMATAYRMLFGHPPHTVKPGDYVLVWGASGGLGVFGVQLAAASGAHVIGVI
CCCCCEEEEEHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEE
SDETKRDYVLGLGAKGVINRKDFKCWGQLPKVNSPEYNEWTKEARKFGKAIWDISGKRDV
CCCCCCCEEEECCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCE
DIVFEHPGEQTFPVSTLVGKRGGMIVFCAGTTGFNITFDARYVWMRQKRIQGSHFAHLKQ
EEEEECCCCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEEEEEEEHHHHCCCCHHHHHHH
ASAANQFIIDRRVDPCMSEVFPWDRIPEAHTKMWKNQHAPGNMAVLVNTPRTGLRTLEDV
HHCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHH
IEAGAKK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]