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The map label for this gene is leuD

Identifier: 154245133

GI number: 154245133

Start: 1362504

End: 1363109

Strand: Reverse

Name: leuD

Synonym: Xaut_1185

Alternate gene names: 154245133

Gene position: 1363109-1362504 (Counterclockwise)

Preceding gene: 154245134

Following gene: 154245132

Centisome position: 25.68

GC content: 64.52

Gene sequence:

>606_bases
ATGGAGAAGTTCACCGTCCTCGAGGGCGTCGCCGCCCCGCTGAAGCTGGTCAATGTCGATACCGACATGATCATCCCCAA
GCAGTACCTCAAGACCATCAAGCGCACGGGCCTCGGCACCGGCCTGTTCTCGGAGATGCGGTACAAGGACGACGGCTCGG
ACAATCCCGACTTCGTGCTCAACCAGAGCGCCTACAAGAATGCCAAGATCCTGGTGGCCGGCGACAATTTCGGCTGCGGC
TCGTCGCGTGAGCACGCGCCCTGGGCGCTGCTCGACTTCGGCATCCGCTGCGTGATCTCCACCTCGTTCGCCGACATCTT
CTACAACAACTGCTTCAAGAACGGCATCCTGCCCGTGGTCGTCTCCAAGGAGGACCTGGACAAGCTGTTCGACGACGCCG
ATCGCGGCGCCAACGCCACCCTCACCATCGACCTGCCGGCGCAGGAGATCCGTGGGCCGGACGGCGGCGTGGTGAAGTTC
GAGATCGACCCGTTCCGCAAGCGCTGCCTCATCGAGGGGCTGGACGACATCGGCCTGACCCTGGAGAAGGCCCCCGCCAT
CGCCGCCTACGAGACCGCCAAGGGCGCCGAGCAGCCCTGGCTGTGA

Upstream 100 bases:

>100_bases
CCTTCGCGCGCCGGTCTTCTTGACTGGGCAAGGGCGGATGCGCCTGTTATGGAGGGGCCTCAGGCAGGAAGCCTAAAGAC
AAGCCGAGGCTCAAGGTCCC

Downstream 100 bases:

>100_bases
GAAGCTGCAGCCCCGCGCGGGGCTGATCGCGGGACGGACGGGACCAAAGCCTCTACGCCTCCGCGATTTACGGGGGCGCT
GGCGGGACCTGAGCCGCTGA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEKFTVLEGVAAPLKLVNVDTDMIIPKQYLKTIKRTGLGTGLFSEMRYKDDGSDNPDFVLNQSAYKNAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVVVSKEDLDKLFDDADRGANATLTIDLPAQEIRGPDGGVVKF
EIDPFRKRCLIEGLDDIGLTLEKAPAIAAYETAKGAEQPWL

Sequences:

>Translated_201_residues
MEKFTVLEGVAAPLKLVNVDTDMIIPKQYLKTIKRTGLGTGLFSEMRYKDDGSDNPDFVLNQSAYKNAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVVVSKEDLDKLFDDADRGANATLTIDLPAQEIRGPDGGVVKF
EIDPFRKRCLIEGLDDIGLTLEKAPAIAAYETAKGAEQPWL
>Mature_201_residues
MEKFTVLEGVAAPLKLVNVDTDMIIPKQYLKTIKRTGLGTGLFSEMRYKDDGSDNPDFVLNQSAYKNAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVVVSKEDLDKLFDDADRGANATLTIDLPAQEIRGPDGGVVKF
EIDPFRKRCLIEGLDDIGLTLEKAPAIAAYETAKGAEQPWL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=200, Percent_Identity=50.5, Blast_Score=197, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321429, Length=208, Percent_Identity=55.2884615384615, Blast_Score=218, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22052; Mature: 22052

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTVLEGVAAPLKLVNVDTDMIIPKQYLKTIKRTGLGTGLFSEMRYKDDGSDNPDFVL
CCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCEEE
NQSAYKNAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVV
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCEEEEEEHHHHHHHHHHHHHCCCEEEE
VSKEDLDKLFDDADRGANATLTIDLPAQEIRGPDGGVVKFEIDPFRKRCLIEGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCCCCEEEEEECHHHHHHHHCCCCHHCCE
LEKAPAIAAYETAKGAEQPWL
EECCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MEKFTVLEGVAAPLKLVNVDTDMIIPKQYLKTIKRTGLGTGLFSEMRYKDDGSDNPDFVL
CCCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCEEE
NQSAYKNAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPVV
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCEEEEEEHHHHHHHHHHHHHCCCEEEE
VSKEDLDKLFDDADRGANATLTIDLPAQEIRGPDGGVVKFEIDPFRKRCLIEGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCCCCEEEEEECHHHHHHHHCCCCHHCCE
LEKAPAIAAYETAKGAEQPWL
EECCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]