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The map label for this gene is ynfL [C]

Identifier: 154245079

GI number: 154245079

Start: 1301845

End: 1302777

Strand: Reverse

Name: ynfL [C]

Synonym: Xaut_1131

Alternate gene names: 154245079

Gene position: 1302777-1301845 (Counterclockwise)

Preceding gene: 154245087

Following gene: 154245073

Centisome position: 24.54

GC content: 69.35

Gene sequence:

>933_bases
ATGGATGTCCGCCAGCTCCGCTATTTCGTCGCCGTGGCGCAGGAGCGCAACTTCACCCGCGCGGCGGAGCGGCTCCACAT
CGCCCAGCCGCCCCTGAGCCGGCAGATCCAGGGGCTGGAGGACGAGCTGGGGGTGATGCTGATCGACCGCTCGGAGCGGC
CGCTGAAGCTCACCGAGGCCGGGCGCTTCTTCTACGAGCAGGCGCTGCAGATGATCGGCCGCATGGACCAGATCCGCAAC
ACCACGCGCCAGCTCGGCCGCTCCGGGCGCAGCCTGTTCGTGGTGGGGTGCGTGGCCTCCACCCTCTATGGCGGCATGCC
GGACCTCGCCCGGCGCATGCGGCGGCTATGGCCGGACATGGAGATCGAGCTGAAGGAAATGAGCTCCACCCAGCAGGTGG
CCGCCCTGAAGGAGGGGCGCATCGACCTCGGCTTCGGCCGCGTGCGCGTCACCGACCCGGCCATCGAGCGCATCACTTTG
CGTGAGGAACGCCTCGTGCTGGCGCTGCCCCTCGACCATCCCAAGCTGGCGCACAAGGGCCCGCTGCCGCTCGCGACGCT
GGAAGACGAGATGCTGGTGCTCTACCCCTCCGAGCCGCGCCCGAGCTTCGCCGACGAGGTGCTGGTGCTGCTCGGCGACC
GCAACGTGCGCCCCCGCGCCATCCGCGAGGTGCGCGAGCTGCAGATGGCGCTGGGGCTGGTGGCGGCGGACGACGGTGTC
ACCATCGTGCCCGCCTCCGCCCAAAGGCTGCGCAGCTACGACGTGGCCTATCGCCATTTCGACGACCCGAAGCTGGTCTC
GCCCATCATCATGAGCTACCGCGCCAATGACGCGTCAGGGCGCATCGAGGCCATCAAGCAGCTGATCCGCGAGATGTACG
CCGACCAGCCCGACTGGCTGAAGCTGTCGGAGAACCGGCTCCTGCCGCCGTGA

Upstream 100 bases:

>100_bases
GTCTGGAGAGGGGGGCATGGATCGTCATGCCCATTTTCTACGGCGCGCCGCGGCGCGGATCCAAAACCGAGTTGGTCAAC
TATTATACTTCGAGGGTATC

Downstream 100 bases:

>100_bases
CCGCGGCGCCGGTCGCTCCTCAACGCAGGCCGAAGCCGAGCGCCTTCAGCACTTCCGGGATGCGGTCGCGCCCGCTTAAG
GACTCCGCCCCGCGCACGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORFR1 [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDVRQLRYFVAVAQERNFTRAAERLHIAQPPLSRQIQGLEDELGVMLIDRSERPLKLTEAGRFFYEQALQMIGRMDQIRN
TTRQLGRSGRSLFVVGCVASTLYGGMPDLARRMRRLWPDMEIELKEMSSTQQVAALKEGRIDLGFGRVRVTDPAIERITL
REERLVLALPLDHPKLAHKGPLPLATLEDEMLVLYPSEPRPSFADEVLVLLGDRNVRPRAIREVRELQMALGLVAADDGV
TIVPASAQRLRSYDVAYRHFDDPKLVSPIIMSYRANDASGRIEAIKQLIREMYADQPDWLKLSENRLLPP

Sequences:

>Translated_310_residues
MDVRQLRYFVAVAQERNFTRAAERLHIAQPPLSRQIQGLEDELGVMLIDRSERPLKLTEAGRFFYEQALQMIGRMDQIRN
TTRQLGRSGRSLFVVGCVASTLYGGMPDLARRMRRLWPDMEIELKEMSSTQQVAALKEGRIDLGFGRVRVTDPAIERITL
REERLVLALPLDHPKLAHKGPLPLATLEDEMLVLYPSEPRPSFADEVLVLLGDRNVRPRAIREVRELQMALGLVAADDGV
TIVPASAQRLRSYDVAYRHFDDPKLVSPIIMSYRANDASGRIEAIKQLIREMYADQPDWLKLSENRLLPP
>Mature_310_residues
MDVRQLRYFVAVAQERNFTRAAERLHIAQPPLSRQIQGLEDELGVMLIDRSERPLKLTEAGRFFYEQALQMIGRMDQIRN
TTRQLGRSGRSLFVVGCVASTLYGGMPDLARRMRRLWPDMEIELKEMSSTQQVAALKEGRIDLGFGRVRVTDPAIERITL
REERLVLALPLDHPKLAHKGPLPLATLEDEMLVLYPSEPRPSFADEVLVLLGDRNVRPRAIREVRELQMALGLVAADDGV
TIVPASAQRLRSYDVAYRHFDDPKLVSPIIMSYRANDASGRIEAIKQLIREMYADQPDWLKLSENRLLPP

Specific function: Probable positive regulator of the cat1 operon which encode enzymes responsible for the degradation of catechol to acetyl-CoA via the beta-ketoadipate pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=34.9206349206349, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1788887, Length=258, Percent_Identity=35.2713178294574, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI2367136, Length=260, Percent_Identity=31.1538461538462, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=29.5546558704453, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI157672245, Length=252, Percent_Identity=32.9365079365079, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=28.1124497991968, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788748, Length=255, Percent_Identity=29.8039215686275, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=146, Percent_Identity=33.5616438356164, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788297, Length=255, Percent_Identity=28.6274509803922, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081904, Length=252, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787806, Length=246, Percent_Identity=24.390243902439, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788296, Length=198, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=207, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=247, Percent_Identity=24.2914979757085, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=26.2295081967213, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788481, Length=191, Percent_Identity=30.3664921465969, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082184, Length=100, Percent_Identity=31, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35349; Mature: 35349

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRQLRYFVAVAQERNFTRAAERLHIAQPPLSRQIQGLEDELGVMLIDRSERPLKLTEA
CCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCHHCCEEEECCCCCCEEHHHH
GRFFYEQALQMIGRMDQIRNTTRQLGRSGRSLFVVGCVASTLYGGMPDLARRMRRLWPDM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHCCCC
EIELKEMSSTQQVAALKEGRIDLGFGRVRVTDPAIERITLREERLVLALPLDHPKLAHKG
EEEHHHHCCHHHHHHHHCCCEEECCCEEEECCHHHHHHEECCCEEEEEECCCCCCCCCCC
PLPLATLEDEMLVLYPSEPRPSFADEVLVLLGDRNVRPRAIREVRELQMALGLVAADDGV
CCCEEECCCCEEEEECCCCCCCHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCC
TIVPASAQRLRSYDVAYRHFDDPKLVSPIIMSYRANDASGRIEAIKQLIREMYADQPDWL
EEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCE
KLSENRLLPP
EECCCCCCCC
>Mature Secondary Structure
MDVRQLRYFVAVAQERNFTRAAERLHIAQPPLSRQIQGLEDELGVMLIDRSERPLKLTEA
CCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCHHCCEEEECCCCCCEEHHHH
GRFFYEQALQMIGRMDQIRNTTRQLGRSGRSLFVVGCVASTLYGGMPDLARRMRRLWPDM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHCCCC
EIELKEMSSTQQVAALKEGRIDLGFGRVRVTDPAIERITLREERLVLALPLDHPKLAHKG
EEEHHHHCCHHHHHHHHCCCEEECCCEEEECCHHHHHHEECCCEEEEEECCCCCCCCCCC
PLPLATLEDEMLVLYPSEPRPSFADEVLVLLGDRNVRPRAIREVRELQMALGLVAADDGV
CCCEEECCCCEEEEECCCCCCCHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCC
TIVPASAQRLRSYDVAYRHFDDPKLVSPIIMSYRANDASGRIEAIKQLIREMYADQPDWL
EEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCE
KLSENRLLPP
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473520 [H]