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The map label for this gene is paaJ [H]

Identifier: 154245070

GI number: 154245070

Start: 1291386

End: 1292591

Strand: Reverse

Name: paaJ [H]

Synonym: Xaut_1122

Alternate gene names: 154245070

Gene position: 1292591-1291386 (Counterclockwise)

Preceding gene: 154245073

Following gene: 154245069

Centisome position: 24.35

GC content: 72.31

Gene sequence:

>1206_bases
ATGAGCGACGCCTTCATCTGTGACGCGGTGCGCACGCCCATCGGGCGCTATGCCGGCGCGCTGGCCTCGGTGCGGCCGGA
CGACCTGGCGGCCCACGTGATCCGCGCCCTCGCCGCGCGCAGCCCCGGCCTCGATGCGGCTCAGGTGGAGGACGTGATCT
TCGGCTGCGCCAACCAGGCCGGCGAGGACAACCGCAACGTGGCCCGCATGGGCGCCCTGCTCTCCGGCCTGCCCATCGAG
GTGCCCGGCTCCACCATCAATCGGCTGTGCGGCTCGGGCATGGATGCGGTGGGCACTGCCGCCCGCGCCATCAAGGCCGG
CGAGACCGACCTCATGATCGCCGGCGGCGTCGAGAGCATGAGCCGCGCCCCCTTCGTCATGGGCAAGGCCGACAGCGCCT
TCTCCCGCGCCGCCAAGATCGAGGACACCACCATCGGCTGGCGCTTCGTGAACCCGGCCATGAAGGCCCTGCACGGGGTC
GATTCCATGCCCGAGACCGCCGAGAACGTGGCGGAGGATTTCCAGATCAGCCGCGCCCGCCAGGACGCCTTCGCGCTGGA
AAGCCAGCGCCGCGCCGCCGAGGCCTGGGCCGCCGGCCGCTTCGCCGACGAGATCGTGGCGGTGAGCATCCCCCAGCGCA
AGGGCGACCCCAAGCTGTTCGACCGCGACGAGCACATGCGCCCCGACACCAAGCTGGAAGACCTCGCCCGCCTCAAGGGC
GTGGTGAAGCCCGGCGGCACCGTGACCGCCGGCAACGCCTCCGGCGTCAATGACGGCGCGGCCGCCCTGCTGCTGGCCTC
CGCCGCCGGGGTGGAGAAGCACGGCCTCACCCCGCGCGGGCGCGTCATCGCCATGGCCACCGCCGGCGTGCCACCGCGCA
TCATGGGCATGGGGCCTGCCCCGGCCACCCGCAAGGTGCTGGAGAAGGCCGGCCTGACACTTGCCGACATGGACGTGATC
GAGCTGAACGAGGCGTTTGCCGCGCAGGCCTGCGCCGTGCTCGCCGACCTCGGCCTGCCGGATGACGCCGCCCATGTGAA
CCCTAACGGCGGCGCCATCGCCCTTGGCCATCCGCTCGGCATGTCGGGGGCGCGGCTCATCACCACCGCGCTCTACCAGC
TCCACCGCACCGGCGGGCGCTACGCCCTCGCCACCATGTGCATCGGCGTCGGCCAGGGCATCGCCACCATCATCGAGCGG
GTGTGA

Upstream 100 bases:

>100_bases
CGTCATCCGAACCTCACGCAAATTTCCCTTGACGCGCACCTGCGCGCGCCCCTATCAATTTTTCGAACTTAAGTTCGTAT
TACGAACAAATGAGGAAGCG

Downstream 100 bases:

>100_bases
CGCCCGCCCCTCCCACTTTCACAAAGAGCGCACCGCCATGCTGTTCCATGTCGAGATGACCGTGAAGATCCCGCACGATT
TTCCGGTCGAGAAGGCCGAC

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSDAFICDAVRTPIGRYAGALASVRPDDLAAHVIRALAARSPGLDAAQVEDVIFGCANQAGEDNRNVARMGALLSGLPIE
VPGSTINRLCGSGMDAVGTAARAIKAGETDLMIAGGVESMSRAPFVMGKADSAFSRAAKIEDTTIGWRFVNPAMKALHGV
DSMPETAENVAEDFQISRARQDAFALESQRRAAEAWAAGRFADEIVAVSIPQRKGDPKLFDRDEHMRPDTKLEDLARLKG
VVKPGGTVTAGNASGVNDGAAALLLASAAGVEKHGLTPRGRVIAMATAGVPPRIMGMGPAPATRKVLEKAGLTLADMDVI
ELNEAFAAQACAVLADLGLPDDAAHVNPNGGAIALGHPLGMSGARLITTALYQLHRTGGRYALATMCIGVGQGIATIIER
V

Sequences:

>Translated_401_residues
MSDAFICDAVRTPIGRYAGALASVRPDDLAAHVIRALAARSPGLDAAQVEDVIFGCANQAGEDNRNVARMGALLSGLPIE
VPGSTINRLCGSGMDAVGTAARAIKAGETDLMIAGGVESMSRAPFVMGKADSAFSRAAKIEDTTIGWRFVNPAMKALHGV
DSMPETAENVAEDFQISRARQDAFALESQRRAAEAWAAGRFADEIVAVSIPQRKGDPKLFDRDEHMRPDTKLEDLARLKG
VVKPGGTVTAGNASGVNDGAAALLLASAAGVEKHGLTPRGRVIAMATAGVPPRIMGMGPAPATRKVLEKAGLTLADMDVI
ELNEAFAAQACAVLADLGLPDDAAHVNPNGGAIALGHPLGMSGARLITTALYQLHRTGGRYALATMCIGVGQGIATIIER
V
>Mature_400_residues
SDAFICDAVRTPIGRYAGALASVRPDDLAAHVIRALAARSPGLDAAQVEDVIFGCANQAGEDNRNVARMGALLSGLPIEV
PGSTINRLCGSGMDAVGTAARAIKAGETDLMIAGGVESMSRAPFVMGKADSAFSRAAKIEDTTIGWRFVNPAMKALHGVD
SMPETAENVAEDFQISRARQDAFALESQRRAAEAWAAGRFADEIVAVSIPQRKGDPKLFDRDEHMRPDTKLEDLARLKGV
VKPGGTVTAGNASGVNDGAAALLLASAAGVEKHGLTPRGRVIAMATAGVPPRIMGMGPAPATRKVLEKAGLTLADMDVIE
LNEAFAAQACAVLADLGLPDDAAHVNPNGGAIALGHPLGMSGARLITTALYQLHRTGGRYALATMCIGVGQGIATIIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=42.8211586901763, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI148539872, Length=399, Percent_Identity=40.8521303258145, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI4501853, Length=404, Percent_Identity=42.5742574257426, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=35.9801488833747, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=32.4009324009324, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI194353979, Length=255, Percent_Identity=32.156862745098, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=69.0773067331671, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=45.4320987654321, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=46.8982630272953, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=44.25, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788683, Length=414, Percent_Identity=32.6086956521739, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=397, Percent_Identity=42.8211586901763, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=34, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=405, Percent_Identity=33.8271604938272, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=32.9466357308585, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=414, Percent_Identity=30.1932367149758, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322031, Length=412, Percent_Identity=38.1067961165049, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6325229, Length=404, Percent_Identity=34.6534653465347, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=45.6359102244389, Blast_Score=327, Evalue=9e-90,
Organism=Drosophila melanogaster, GI17648125, Length=400, Percent_Identity=39, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=35.7142857142857, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=32.1596244131455, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41518; Mature: 41387

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAFICDAVRTPIGRYAGALASVRPDDLAAHVIRALAARSPGLDAAQVEDVIFGCANQA
CCCCEEHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVARMGALLSGLPIEVPGSTINRLCGSGMDAVGTAARAIKAGETDLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPFVMGKADSAFSRAAKIEDTTIGWRFVNPAMKALHGVDSMPETAENVAEDFQISRAR
CCCCEEEECCCHHHHHHHCCCCCCCCEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QDAFALESQRRAAEAWAAGRFADEIVAVSIPQRKGDPKLFDRDEHMRPDTKLEDLARLKG
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VVKPGGTVTAGNASGVNDGAAALLLASAAGVEKHGLTPRGRVIAMATAGVPPRIMGMGPA
CCCCCCEEECCCCCCCCCHHHHHHHHHHCCCHHCCCCCCCCEEEEEECCCCCCEEECCCC
PATRKVLEKAGLTLADMDVIELNEAFAAQACAVLADLGLPDDAAHVNPNGGAIALGHPLG
HHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCC
MSGARLITTALYQLHRTGGRYALATMCIGVGQGIATIIERV
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
SDAFICDAVRTPIGRYAGALASVRPDDLAAHVIRALAARSPGLDAAQVEDVIFGCANQA
CCCEEHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVARMGALLSGLPIEVPGSTINRLCGSGMDAVGTAARAIKAGETDLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPFVMGKADSAFSRAAKIEDTTIGWRFVNPAMKALHGVDSMPETAENVAEDFQISRAR
CCCCEEEECCCHHHHHHHCCCCCCCCEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QDAFALESQRRAAEAWAAGRFADEIVAVSIPQRKGDPKLFDRDEHMRPDTKLEDLARLKG
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VVKPGGTVTAGNASGVNDGAAALLLASAAGVEKHGLTPRGRVIAMATAGVPPRIMGMGPA
CCCCCCEEECCCCCCCCCHHHHHHHHHHCCCHHCCCCCCCCEEEEEECCCCCCEEECCCC
PATRKVLEKAGLTLADMDVIELNEAFAAQACAVLADLGLPDDAAHVNPNGGAIALGHPLG
HHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCC
MSGARLITTALYQLHRTGGRYALATMCIGVGQGIATIIERV
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]