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The map label for this gene is bigR [H]

Identifier: 154245054

GI number: 154245054

Start: 1277594

End: 1277905

Strand: Direct

Name: bigR [H]

Synonym: Xaut_1106

Alternate gene names: 154245054

Gene position: 1277594-1277905 (Clockwise)

Preceding gene: 154245040

Following gene: 154245057

Centisome position: 24.06

GC content: 66.99

Gene sequence:

>312_bases
ATGAATGTTGTTGATATGATGCCCGCTTCGGAACGGGCGGCCGAGCTGATGCGCAGCCTCTCCCATCCCCAGCGCCTTCT
GGTGCTGTGCTCCCTGGGCAGCGGGGAAAAGTCGGTGGGCCAATTGCGGGACGAACTGGGCATCGAGCAGGTGCCCATGT
CGCAGCAGCTCATGCGCCTGCGGGCCGACGGGCTGGTGGAGGCGCGGCGCGAGGGCACGAGCGTGCACTACCGCATCGCC
CGCGAGGAGGTGCTCCATATCATCGAGGCGCTGCACAGGGCCTTCTGCAGCAAGCCGGAGGGGCCGTGCTGA

Upstream 100 bases:

>100_bases
GCGATCAACCGACATTATCTTGTTCCCTTGAAAACGCGCGAGCCCCGTGCCCGTGCGTGTTCGTTTGCACTTAACATTCA
AACATTATAAATTCAAGATC

Downstream 100 bases:

>100_bases
GCGGTGTGAGGCTTAACCCTCTCTGGGGCGCCAGACGCTGGTGCGCTTGATCTCCATGTCCTCCAGCTCCCGGGTCACCG
GCACGCTGTATTCGGCGAGA

Product: regulatory protein ArsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 103; Mature: 103

Protein sequence:

>103_residues
MNVVDMMPASERAAELMRSLSHPQRLLVLCSLGSGEKSVGQLRDELGIEQVPMSQQLMRLRADGLVEARREGTSVHYRIA
REEVLHIIEALHRAFCSKPEGPC

Sequences:

>Translated_103_residues
MNVVDMMPASERAAELMRSLSHPQRLLVLCSLGSGEKSVGQLRDELGIEQVPMSQQLMRLRADGLVEARREGTSVHYRIA
REEVLHIIEALHRAFCSKPEGPC
>Mature_103_residues
MNVVDMMPASERAAELMRSLSHPQRLLVLCSLGSGEKSVGQLRDELGIEQVPMSQQLMRLRADGLVEARREGTSVHYRIA
REEVLHIIEALHRAFCSKPEGPC

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=95, Percent_Identity=36.8421052631579, Blast_Score=69, Evalue=5e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 11524; Mature: 11524

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVDMMPASERAAELMRSLSHPQRLLVLCSLGSGEKSVGQLRDELGIEQVPMSQQLMRL
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
RADGLVEARREGTSVHYRIAREEVLHIIEALHRAFCSKPEGPC
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNVVDMMPASERAAELMRSLSHPQRLLVLCSLGSGEKSVGQLRDELGIEQVPMSQQLMRL
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
RADGLVEARREGTSVHYRIAREEVLHIIEALHRAFCSKPEGPC
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]